An additional case of a new strain of mpox has been detected in Britain, bringing the total number of infections in the country to four.

Neuronal cytotoxic edema is implicated in neuronal injury and death, yet mitigating brain edema with osmotic and surgical interventions yields poor clinical outcomes. Importantly, neuronal swelling and its downstream consequences during early brain development remain poorly investigated, and new treatment approaches are needed. We explored Ca²⁺-dependent downstream effects after neuronal cytotoxic edema caused by diverse injuries in mice of both sexes using multiphoton Ca²⁺ imaging in vivo [Postnatal Day (P)12–17] and in acute brain slices (P8–12). After different excitotoxic insults, cytosolic GCaMP6s translocated into the nucleus after a few minutes in a subpopulation of neurons, persisting for hours. We used an automated morphology-detection algorithm to detect neuronal soma and quantified the nuclear translocation of GCaMP6s as the nuclear to cytosolic intensity (N/C ratio). Elevated neuronal N/C ratios occurred concurrently with persistent elevation in Ca²⁺ loads and could also occur independently from neuronal swelling. Electron microscopy revealed that the nuclear translocation was associated with the increased nuclear pore size. The nuclear accumulation of GCaMP6s in neurons led to neocortical circuit dysfunction, mitochondrial pathology, and increased cell death. Inhibiting calpains, a family of Ca²⁺-activated proteases, prevented elevated N/C ratios and neuronal swelling. In summary, in the developing brain, we identified a calpain-dependent alteration of nuclear transport in a subpopulation of neurons after disease-relevant insults leading to long-term circuit dysfunction and cell death. The nuclear translocation of GCaMP6 and other cytosolic proteins after acute excitotoxicity can be an early biomarker of brain injury in the developing brain.

Injuries to the central nervous system (CNS) can cause severe neurological deficits. Axonal regrowth is a fundamental process for the reconstruction of compensatory neuronal networks after injury; however, it is extremely limited in the adult mammalian CNS. In this study, we conducted a loss-of-function genetic screen in cortical neurons, combined with a Web resource-based phenotypic screen, and identified synaptotagmin 4 (Syt4) as a novel regulator of axon elongation. Silencing Syt4 in primary cultured cortical neurons inhibits neurite elongation, with changes in gene expression involved in signaling pathways related to neuronal development. In a spinal cord injury model, inhibition of Syt4 expression in cortical neurons prevented axonal sprouting of the corticospinal tract, as well as neurological recovery after injury. These results provide a novel therapeutic approach to CNS injury by modulating Syt4 function.

Megan Leftwich, an engineering professor at George Washington University, is building a robotic flipper based on her observations of sea lions

In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

Integrated Tools Make Mechatronics-Oriented Design and Seamless Deployment to Hardware Easy

Customers highlight ability to see flow of gasses, liquids, and heat helps designers anticipate and correct flaws early in the design process

‘To design, I use my favorite tool, SOLIDWORKS’

A newly-discovered iPhone 14 Pro prototype features one of the earliest known incarnations of Apple's scrapped haptic button project.

On an earlier occasion, Shaan had taken a dig at new-age singers highlighting how they have shifted to "minimalistic" songs

NEW CASTLE, DE (March 28, 2022) – The Council on Development Finance (CDF) on Monday approved eight Site Readiness Fund project applications totaling $6.2 million. The projects are located throughout the state with three each in New Castle County and Kent County and two in Sussex County. Established through Senate Bill 127, the Site Readiness […]

Wilmington, Del. (June 6, 2023) – Five organizations in the State of Delaware will receive a total of $1,030,000 in federal grants from the National Endowment for the Arts, as part of the National Endowment for the Arts’ second round of funding, announced today. “The National Endowment for the Arts is pleased to support a […]

Potential victims encouraged to contact (302) 577-5293 or ReportAbuse@delaware.gov   Tyrone Clark, 64, has been sentenced to a total of 125 years for the rape and abuse of a 12-year-old child in his care.  “No crime is more offensive, more heinous, and more dangerous than the sexual abuse of a child,” said Attorney General Jennings. “I’m grateful to […]

Delawareans struggling with substance use disorder can call DHSS’ Division of Substance Abuse and Mental Health’s 24/7 Delaware Hope Line – a single point of contact where callers can connect to a variety of resources and information, including support from clinicians and peer specialists plus crisis assistance.

Health Officials Fear Opioid Epidemic Has Not Plateaued   NEW CASTLE (Dec. 8, 2022) – In a heartbreaking sign that the opioid epidemic has not plateaued in Delaware, deaths from suspected overdoses in November set a new monthly high total, according to death investigation reports from the Division of Forensic Science. In November 2022, 43 […]

As the Thanksgiving holiday approaches, Delaware now has 139 traffic fatalities, reaching last year’s total of 139, the highest number since 2006. “Thousands of Delawareans will take to the roads in the coming days and weeks to visit friends and family near and far and no matter your destination, we remind drivers to put their […]

Attorney General Kathy Jennings today joined a coalition of 50 states and territories announcing two significant cooperation agreements and settlements with Heritage Pharmaceuticals and Apotex totaling $49.1 million to resolve allegations that both companies engaged in widespread, long-running conspiracies to artificially inflate and manipulate prices, reduce competition, and unreasonably restrain trade with regard to numerous generic prescription […]

Check here each week to keep up with the latest from John MacArthur's pulpit at Grace Community Church.

Vice President Kashim Shettima has stated that trade facilitation programmes implemented by the current administration increased the country’s trade balance to N6.5tn in the second quarter of 2024. He said efforts to maximise economic opportunities ensured that exports to other countries accounted for 60.89 per cent, or equivalent to N19.42tn of total trade, representing a

Title: Picture of Phototoxic Dermatitides
Category: Images
Created: 2/23/2010 2:33:00 PM
Last Editorial Review: 6/28/2022 12:00:00 AM

In Canada and across the globe, access to PSMA PET/CT is limited and expensive. For patients with biochemical recurrence (BCR) after treatment for prostate cancer, novel strategies are needed to better stratify patients who may or may not benefit from a PSMA PET scan. The role of the free-to-total prostate-specific antigen (PSA) ratio (FPSAR) in posttreatment prostate cancer, specifically in the PSMA PET/CT era, remains unknown. Our aim in this study was to determine the association of FPSAR in patients referred for ¹⁸F-DCFPyL PSMA PET/CT in the BCR setting and assess the correlation between FPSAR and ¹⁸F-DCFPyL PSMA PET/CT positivity (local recurrence or distant metastases). Methods: This prospective study included 137 patients who were referred for ¹⁸F-DCFPyL PSMA PET/CT and had BCR with a total PSA of less than 1 ng/mL after radical prostatectomy (RP) (including adjuvant or salvage radiotherapy). Blood samples were collected on the day of ¹⁸F-DCFPyL PSMA PET/CT. FPSAR was categorized as less than 0.10 or as 0.10 or more. A positive ¹⁸F-DCFPyL PSMA PET/CT scan was defined by a PROMISE classification lesion score of 2 or 3, irrespective of the site of increased tracer uptake (e.g., prostate, pelvic nodes, bone, or viscera). Results: Overall, 137 blood samples of patients with BCR after RP were analyzed to calculate FPSAR. The median age at ¹⁸F-DCFPyL PSMA PET/CT was 68.6 y (interquartile range, 63.0–72.4 y), and the median PSA at ¹⁸F-DCFPyL PSMA PET/CT was 0.3 ng/mL (interquartile range, 0.3–0.6 ng/mL). Eighty-six patients (62.8%) had an FPSAR of less than 0.10, whereas 51 patients (37.2%) had an FPSAR of 0.10 or more. An FPSAR of 0.10 or more was identified as an independent predictor of a positive ¹⁸F-DCFPyL PSMA PET/CT scan, with an odds ratio of 6.99 (95% CI, 2.96–16.51; P < 0.001). Conclusion: An FPSAR of 0.10 or more after RP independently correlated with increased odds of a positive ¹⁸F-DCFPyL PSMA PET/CT scan among BCR post-RP patients. These findings may offer an inexpensive method by which to triage access to ¹⁸F-DCFPyL PSMA PET/CT in jurisdictions where availability is not replete.

Ogres, Orcs, and Khorne are all on the way in the upcoming expansion for strategy game Total War: Warhammer 3, and Creative Assembly have just released their latest dev vlog with a few more details on what to expect. There’s still no word on the exact title, although given the established naming convention (Shadows Of Change, Thrones Of Decay), I’m tentatively calling it “Sniffers Of Glue” in honour of the No Think, Only Krump faction selection.

You’ll find the vlog in its full glory below. What’s interesting about this one is that vlog mainstay director Rich Alridge has brought along some new faces: battle designer Josh King and audio director Chris Goldsmith. And, yes, so no-one can accuse me of burying the lede: that audio design involved the enthusiastic, deeply disgusting slurping of porridge and yoghurt, and the jangling of real bones. The source of the bones is not revealed.

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EA FC 25 players have a whole new Team of the Week to grind for, and there are some fantastic cards among them, including Sporting CP's hotshot striker Viktor Gyokeres.

The California-based Middlebury Institute of International Studies published research this week that showed China has constructed a prototype nuclear reactor that could fit aboard a large surface vessel - a sign that China is making progress toward building a nuclear-powered aircraft carrier.

The post Report: China Builds ‘Prototype’ Nuclear Reactor for Aircraft Carrier appeared first on Breitbart.

Summer holidays might be over, but fans of Gunship Battle: Total Warfare are still in for a treat as Joycity …

Continue reading "‘Gunship Battle: Total Warfare’ Launches Massive Sky Ace Update"

Feral Interactive’s tease seemingly was about Total War: Empire from Creative Assembly and SEGA because the developer just announced that …

Continue reading "‘Total War: Empire’ Is Coming to iOS and Android This Fall From Feral Interactive"

Robert Musselle, customer engineering manager EMEA at Protolabs shares what you need to consider with 3D printing and plastics prototyping.

A grand prize of $10 million is up for grabs at the upcoming Toto draw on Thursday (Nov 14) at 9.30pm. The Group 1 prize money has snowballed over the past three draws after no Group 1 winners were picked. According to Singapore Pools' website, the prize started at $1.2 million on Nov 4 before snowballing to $2.9 million on Nov 7 and $6 million on Nov 11. The last draw on Monday yielded three Group 2 winners who won $215,010 each. Tomorrow's draw will be a cascade draw, meaning that the prize money will be split between the Group 2 winners in the event that no Group 1 winner is chosen. If there are no Group 2 winners, the prize will be split among the Group 3 winners, and so on. The last time a Group 1 prize snowballed above $10 million was during the October 21 draw where two lucky winners bagged over $6.6 million each.

Brad Hiranga, General Mills Business Unit Director, Pizza and Tacos Business Unit discusses the Bucking Couch and Bucking Couch Bowl.

Autotrader just announced the list of 2016 Must Test Drive vehicles. NASCAR driver Brad Keselowski�s #2 racecar is not on the list�and he thinks it should be. Find out why.

An Epic Summer Adventure Awaits

Sleep Inn�s new prototype design unveiled at the Choice Hotels 62nd Annual Convention in Las Vegas

El avance de Habla Men

Joey Fatone: IN SINK

Comet 12P/Pons-Brooks will make its closest approach to the sun this April—right after North America is treated to a total solar eclipse

Once upon a time a man named Barry Goldwater appeared on the political scene. And the radical cried, “Danger! A fascist!” And the people came running, but they saw that, actually, he was just a right-wing authoritarian, and he was making no effort to build a mass movement based on violence and terror in order … Continue reading A Totally Original Parable Not Derived From Anything Else Really

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Foxconn, the Taiwanese company known for assembling Apple products, has unveiled three electric-vehicle prototypes. WSJ’s Stephanie Yang attended the launch event to see how the company is diversifying its business to gain a foothold in the auto industry. Photo: I-Hwa Cheng/Bloomberg News

London : Bloomsbury Academic, 2020