The married couple goes on Instagram Live as a part of their new series 'The Biebers on Watch', doing a fun QnA session where they discuss Justin's struggle with Lyme disease.

The married couple goes on Instagram Live as a part of their new series 'The Biebers on Watch', doing a fun QnA session where they discuss Justin's struggle with Lyme disease.

The FBI reports that hate crimes in Connecticut and on Long Island went down last year.

It’s gameday in Denver. Before the Broncos start playing football, players are announced as they sprint onto the field through a smoke-filled tunnel shaped like three wild, galloping horses.

Can you find compassion in your heart for the haters in your life?

In the wake of the protests by neo-Nazis and white supremacists that turned deadly in Charlottesville, Virginia, President Donald Trump has come under fire for not immediately and clearly condemning American racists. It’s not the first time. Trump and those close to him have often played down the threat of violence committed by white supremacists across the country.

This week, through interviews with key Trump supporters and advisers, we explore if we should have seen Charlottesville coming and if we should expect more race-based clashes on the way.

To explore more reporting, visit revealnews.org or find us at fb.com/ThisIsReveal, on Twitter @reveal or Instagram @revealnews.

There’s been a lot of conversation about whether Donald Trump has inspired a new wave of hate in America.

Reveal reporter Will Carless set out to understand the president’s role in hundreds of hate incidents across the country, with help from the Documenting Hate project led by ProPublica. He found a striking pattern that extended across races, religions and sexual orientation. We also examine what’s going on inside the government agency that’s supposed to be fighting discrimination, the Justice Department’s Civil Rights Division. And we ask what it takes for bystanders in hate speech incidents to become allies.

Don’t miss the next big story. Get the Weekly Reveal newsletter today.

This episode surveys the state of the hate movement in America, focusing on how hate groups are spreading their message and winning converts.

Don’t miss out on the next big story. Get the Weekly Reveal newsletter today.

In the second part of Reveal’s series about hate, we look at how racism and white supremacy are institutionalized in America.

Don’t miss out on the next big story. Get the Weekly Reveal newsletter today.

In 2014, a pair of crimes shocked Israelis and Palestinians. The first was the abduction and murder of three Israeli boys by a Hamas-linked group. Then there was an act of reprisal—the torture, burning, and murder of a Palestinian teen-ager named Mohammed Abu Khdeir—by Israeli right-wing extremists. Even by the standards of this conflict, the killings were shocking. 

“Our Boys,” a co-production of HBO and the Israeli Keshet Studios, examines the forces that led to Abu Khdeir’s killing. It is not for the faint of heart, David Remnick says, but the series is as complex and deep a portrayal of the conflict as he has ever seen. Remnick spoke with two of the creators: Hagai Levi, an Israeli Jew, and Tawfiq Abu Wael, a Palestinian living in Israel. Abu Wael tells Remnick why he resisted pressure from activists not to participate in an Israeli production. 

Updated Nov. 12, 5:25 p.m. ET While the number of reported hate crimes dipped slightly in 2018, violence against individuals rose to a 16-year high, according to numbers released Tuesday by the FBI. The FBI's annual tally counted 7,120 hate crimes reported last year, 55 fewer than the year before. The main concern for extremism trackers, however, is the rising level of violence — the report showed an increase in the number of "crimes against persons," such as intimidation, assault and homicide. "We're seeing a leaner and meaner type of hate crime going on," said Brian Levin, director of the Center for the Study of Hate and Extremism at California State University San Bernardino. "Homicides were up and crimes against persons were up and that's an important thing to look at." Hate crimes targeting people accounted for 61% of all hate crimes in 2018, according to Levin, who is co-author of a report released Tuesday that analyzes law enforcement data. The FBI recorded 24 murders classified

Special guest Ben Thompson returns to the show to celebrate the 10th anniversary of the iPad.

Buying in the suburbs vs renting in the city? We are living in Vancouver right now, and we love a lot about it, but we could buy a place in the suburbs right now (which might not be true six months or a year from now). We are really torn, and I want some perspective on what moving to the suburbs is really like, and if owning is that much better than renting.

We've been renting a flat in East Vancouver for a year and love a lot of things about it. The proximity to work downtown, the neighbourhood feel, proximity to beaches and attractions, the kids' school (both elementary-aged), cherry blossoms, shopping, all the things people love about Vancouver.

We haven't been saving any money though, because our rent is outrageously high. We can buy a 2000 sq ft condo in Surrey for less than the rent of 1000 sq ft in East Van. We have a small down-payment saved up, but we're not adding to it anymore, so if we are going to buy now is the time. There are some very motivated sellers at the moment and prices have come down, which they NEVER do in the area.

But we are torn. Suburbs mean longer commute (and paying for transit instead of biking to work), longer travel time to all the fun things we love, changing the kids' school, further to the airport/ferry, the awfulness of moving, etc. We would gain some space, some privacy, some autonomy (paint walls! get a hamster!) and some equity.

Have you moved to the suburbs with kids? Was it worth it?

Additional details: I'm a stay-at-home-mom and my wife works right downtown in Vancouver. Both of our kids have ADHD and are ROWDY. Moving to another (cheaper) rental is out-of-the-question. Even though our current place isn't perfect, its good enough that if we continue to rent we just wanna stay here. If we bought, it would be into a strata, with all that entails. We have owned a house before but not in this province.

The story of one man's slide into the white supremacist movement in Canada in the late 1980s, and which asks the question; whose voices should be heard in the aftermath of violence, as a community attempts to move towards life after hate?

The story of one man's slide into the white supremacist movement in Canada, and the aftermath. How do we deal with those who've engaged in the politics of hate when they decide to walk away from it?

Are you feeling torn about celebrating the Melbourne Cup today? We asked our ABC Messenger audience how they felt about the race that stops the nation and what they loved (and hated) about it.

A section of an ABC television program titled Women Feel Guilty Whatever They Do, aired on March 27, 1985. It features stay-at-home mother Liz Crawford and working mother Deborah Brennan.

Melbourne tea shop manager Jennifer Li recorded a stranger hurling racially charged taunts at her when she tried to defend herself and customers who were wearing facemasks.

Democratic state lawmakers say hate and bigotry are on the rise during the COVID-19 pandemic and they’re asking Coloradans to join them in condemning it.

The rapper however does not apologize.

Lawmakers are reacting with outrage to the February killing

Lawmakers are reacting with outrage to the February killing

Channel your inner Purple One with these iconic gifs.

I live in Stockport, just outside Manchester. It's 10 minutes by train away, but I’m not sure if the trains are running – and in any case I’ve not actually been in my office in Central Manchester since February 20.
That got complex. I was in Iraq for work and came home in early March with a virus. Just not that virus but they wouldn’t test me because Iraq (you know, right next door to Iran) wasn’t on the WHO list.
So. Context. We live in a suburban semi-detatched house with a garden (big for Edgeley). There’s me. Matt the husband.…

BACKGROUND Glucose-6-phosphate dehydrogenase (G6PD) deficiency decreases the ability of red blood cells (RBCs) to withstand oxidative stress. Refrigerated storage of RBCs induces oxidative stress. We hypothesized that G6PD-deficient donor RBCs would have inferior storage quality for transfusion as compared with G6PD-normal RBCs.METHODS Male volunteers were screened for G6PD deficiency; 27 control and 10 G6PD-deficient volunteers each donated 1 RBC unit. After 42 days of refrigerated storage, autologous 51-chromium 24-hour posttransfusion RBC recovery (PTR) studies were performed. Metabolomics analyses of these RBC units were also performed.RESULTS The mean 24-hour PTR for G6PD-deficient subjects was 78.5% ± 8.4% (mean ± SD), which was significantly lower than that for G6PD-normal RBCs (85.3% ± 3.2%; P = 0.0009). None of the G6PD-normal volunteers (0/27) and 3 G6PD-deficient volunteers (3/10) had PTR results below 75%, a key FDA acceptability criterion for stored donor RBCs. As expected, fresh G6PD-deficient RBCs demonstrated defects in the oxidative phase of the pentose phosphate pathway. During refrigerated storage, G6PD-deficient RBCs demonstrated increased glycolysis, impaired glutathione homeostasis, and increased purine oxidation, as compared with G6PD-normal RBCs. In addition, there were significant correlations between PTR and specific metabolites in these pathways.CONCLUSION Based on current FDA criteria, RBCs from G6PD-deficient donors would not meet the requirements for storage quality. Metabolomics assessment identified markers of PTR and G6PD deficiency (e.g., pyruvate/lactate ratios), along with potential compensatory pathways that could be leveraged to ameliorate the metabolic needs of G6PD-deficient RBCs.TRIAL REGISTRATION ClinicalTrials.gov NCT04081272.FUNDING The Harold Amos Medical Faculty Development Program, Robert Wood Johnson Foundation grant 71590, the National Blood Foundation, NIH grant UL1 TR000040, the Webb-Waring Early Career Award 2017 by the Boettcher Foundation, and National Heart, Lung, and Blood Institute grants R01HL14644 and R01HL148151.

I'm relatively new to the world of Instructional Design and the development of eLearning material, but I've had a LOT of experience as a trainee. Much of that experience has been pretty painful (this should be no surprise to anyone). If you, as an Instructional Designer or course developer, want to keep me engaged in […]

Reports of hate crime in Scotland have increased and research indicates that the trauma experienced by victims of hate crime can be more enduring and harmful than non-hate related offending and that it has detrimental effects on communities as well as individuals. There is a clear role for criminal justice social work in this area in working with perpetrators.

Rania Hamad, City of Edinburgh Council spoke to us about her research on the topic. It aims to:

• define 'hate crime' and highlight the complexities around definitions
• provide an understanding of the scope and nature of hate crime in Scotland and Edinburgh
• explore the causes of hate crime, including individual and wider structural causation
• outline the 'characteristics' of hate crime perpetrators including a discussion around risk assessment
• explore what can be learned from hate crime interventions
• explore 'best practice' for practitioners in this area of work
• highlight gaps in current knowledgeRead: Hate crime: causes, motivations and effective interventions for criminal justice social work.

Transcript of episode

Music Credit: Something Elated by Broke For Free

The Clippers have surpassed the Celtics in terms of being the Lakers' biggest and most-hated rival in the eyes of some fans. The teams play again on Sunday.

Profits from the sale of the hotel's bathrobes, keychains and designer-collaboration cashmere sweaters will go to a fund for fired workers.

Joe Biden is a flawed individual with a penchant for unwanted touching. Here's why I'll vote for him anyway.

Professors don't want us living in single-family homes, the only option for average people to own something all their own.

I've spent 20 years covering the Oscars, and yes, they're splendid and ridiculous, flawed and inspirational. That's why they still matter.

Just above the Sunset Strip, a 1920s villa once owned by actor James Franco and filmmaker Francis Lawrence just sold for $4.65 million.

TEXTILES: brace yourselves. We have a new hate crime on our books.

QUEEN ELIZABETH II, 94, is the world's best-travelled monarch and has visited countries all over the world. Prince Philip, 98, often joins her on her many travels - but there's one thing he hates.

On 29 July 2019, we published an article originally headlined "Islamic Caliphate in rural England: 35000 to pledge allegiance in mass conversion ceremony".

Gregory and Travis McMichael, who are accused of fatally shooting Ahmaud Arbery, a black man, will not face hate crime charges. Here's why.

The alarming rise of anti-Semitism in this country and other parts of the world is completely unacceptable, a letter to the editor says.

The municipality of Chateauguay is advising residents to boil tap water starting Monday as work will begin on the water system.

People are mad because some big companies got loans through a program meant to help mom-and-pop businesses.

“It’s just so unfair that American companies aren’t allowed to pay bribes to get business overseas.”

Eiji Kinoshita
Apr 1, 2006; 5:749-757
Technology

The transfer of a phosphate from ATP to a protein substrate, a modification known as protein phosphorylation, is catalyzed by protein kinases. Protein kinases play a crucial role in virtually every cellular activity. Recent studies of atypical protein kinases have highlighted the structural similarity of the kinase superfamily despite notable differences in primary amino acid sequence. Here, using a bioinformatics screen, we searched for putative protein kinases in the intracellular bacterial pathogen Legionella pneumophila and identified the type 4 secretion system effector Lpg2603 as a remote member of the protein kinase superfamily. Employing an array of biochemical and structural biology approaches, including in vitro kinase assays and isothermal titration calorimetry, we show that Lpg2603 is an active protein kinase with several atypical structural features. Importantly, we found that the eukaryote-specific host signaling molecule inositol hexakisphosphate (IP6) is required for Lpg2603 kinase activity. Crystal structures of Lpg2603 in the apo-form and when bound to IP6 revealed an active-site rearrangement that allows for ATP binding and catalysis. Our results on the structure and activity of Lpg2603 reveal a unique mode of regulation of a protein kinase, provide the first example of a bacterial kinase that requires IP6 for its activation, and may aid future work on the function of this effector during Legionella pathogenesis.

The transfer of a phosphate from ATP to a protein substrate, a modification known as protein phosphorylation, is catalyzed by protein kinases. Protein kinases play a crucial role in virtually every cellular activity. Recent studies of atypical protein kinases have highlighted the structural similarity of the kinase superfamily despite notable differences in primary amino acid sequence. Here, using a bioinformatics screen, we searched for putative protein kinases in the intracellular bacterial pathogen Legionella pneumophila and identified the type 4 secretion system effector Lpg2603 as a remote member of the protein kinase superfamily. Employing an array of biochemical and structural biology approaches, including in vitro kinase assays and isothermal titration calorimetry, we show that Lpg2603 is an active protein kinase with several atypical structural features. Importantly, we found that the eukaryote-specific host signaling molecule inositol hexakisphosphate (IP6) is required for Lpg2603 kinase activity. Crystal structures of Lpg2603 in the apo-form and when bound to IP6 revealed an active-site rearrangement that allows for ATP binding and catalysis. Our results on the structure and activity of Lpg2603 reveal a unique mode of regulation of a protein kinase, provide the first example of a bacterial kinase that requires IP6 for its activation, and may aid future work on the function of this effector during Legionella pathogenesis.

Gernot F. Grabner
Apr 29, 2020; 0:jlr.RA119000516v1-jlr.RA119000516
Research Articles

Bis(monoacylglycero)phosphate (BMP), also known as lysobisphosphatidic acid (LBPA), is a phospholipid that promotes lipid sorting in late endosomes/lysosomes by activating lipid hydrolases and lipid transfer proteins. Changes in the cellular BMP content therefore reflect an altered metabolic activity of the endo-lysosomal system. Surprisingly, little is known about the physiological regulation of BMP. In this study, we investigated the effects of nutritional and metabolic factors on BMP profiles of whole tissues and  parenchymal and non-parenchymal cells. Tissue samples were obtained from fed, fasted, two-hours refed, and insulin-treated mice, as well as from mice housed at  5°C, 22°C, or 30°C. These tissues exhibited distinct BMP profiles, which were regulated by the nutritional state in a tissue-specific manner. Insulin treatment was not sufficient to mimic refeeding-induced changes in tissue BMP levels indicating that BMP metabolism is regulated by other hormonal or nutritional factors. Tissue fractionation experiments revealed that fasting drastically elevates BMP levels in hepatocytes and pancreatic cells. Furthermore, we observed that the BMP content in brown adipose tissue strongly depends on housing temperatures. In conclusion, our observations suggest that BMP concentrations adapt to the metabolic state in a tissue-and cell type-specific manner in mice. Drastic changes observed in hepatocytes, pancreatic cells, and brown adipocytes suggest that BMP possesses a role in the functional adaption to nutrient starvation and ambient temperature.