A podcast launch can be fun and jumpstart momentum for growth. Starting your podcasting with multiple episodes is often advised, but is it right for you?

Feed hits and monthly downloads are two podcast stats the may confuse or mislead podcasters. Here's why you should never rely on these meaningless numbers.

Working with other podcasters can be energizing, but it can also feed your inner troll. Here's how you can build friendships instead of enemies in podcasting.

Many podcasting tools offer the ability to automatically crosspost your audio podcast to YouTube. Here are eleven reasons I think you shouldn't do that.

A podcast failure doesn't mean you should quit podcasting. Here are 9 steps to help you recover and keep moving forward!

Apple is cracking down on keyword-stuffing in podcast tags. Here's information from testing and experience to help you protect your podcast!

Podcast media and feed hosting performance comparisons between the most popular and some not-so-popular podcast hosting providers.

Podcasters need a WordPress theme designed with features and flexibility to support podcasting. Here's why I think SecondLine Themes are your new best choice!

If you're frustrated by your WordPress theme's limitations, you don't know how to or don't want to write custom code, or you want a lot more flexibility in your website, you might want to consider a page-builder plugin for WordPress. Benefits of page-builders 1. You don't have to know HTML, CSS, PHP, or JavaScript to...

Switching to the Gutenberg Editor was probably the most controversial change in WordPress's history. I'll help you decide whether you should start using Gutenberg for your podcast's WordPress website.

On December 10, 2019, Apple Podcasts surpassed 800,000 valid podcasts! Here's some more information and statistics on the podcast industry, with data from My Podcast Reviews.

http://www.musicxray.com/xrays/1319868 CAROLINA CLAY - cc1premixmp3

http://www.musicxray.com/xrays/1319869 CAROLINA CLAY - cc4premixmp3

http://www.musicxray.com/xrays/1319870 CAROLINA CLAY - cc5premixmp3

Years ago, when Fullmetal Alchemist (the first one) aired, someone released an mp3 of the seiyuu/voice actor for Roy Mustang either counting sheep or just plain counting in Japanese, probably from 1 to 100 or similar. It was extremely soothing. I have since lost my copy, and haven't had any luck finding it on the internet--does anyone know where to find this audio file?

(United States Second Circuit) - In a copyright infringement action brought by record companies and music publishers against internet music services that allowed users to search for free music, dealing with the requirement of the Digital Millennium Copyright Act (DMCA) safe harbor that an internet service provider adopt and reasonably implement a policy to terminate repeat infringers, under 17 U.S.C. section 512, the District Court's grant of partial summary judgment in favor of defendants and decision overturning a jury verdict in favor of plaintiffs is: 1) vacated as to partial summary judgment to the defendants based on the conclusion that defendant qualified for safe harbor protection under the DMCA because the District Court applied too narrow a definition of 'repeat infringer'; 2) reversed as to judgment as a matter of law to the defendants on claims that defendant permitted infringement of plaintiffs' copyrights in pre‐2007 MP3s and Beatles songs because there was sufficient evidence to allow a reasonable jury to conclude that defendant had red‐flag knowledge of, or was willfully blind to, infringing activity involving those categories of protected material; 3) remanded for further proceedings related to claims arising out of the District Court’s grant of partial summary judgment; and 4) affirmed in all other respects.

(United States Second Circuit) - In an amended opinion involving a copyright infringement action brought by record companies and music publishers against internet music services that allowed users to search for free music, dealing with the requirement of the Digital Millennium Copyright Act (DMCA) safe harbor that an internet service provider adopt and reasonably implement a policy to terminate repeat infringers, under 17 U.S.C. section 512, the District Court's grant of partial summary judgment in favor of defendants and decision overturning a jury verdict in favor of plaintiffs is: 1) vacated as to partial summary judgment to the defendants based on the conclusion that defendant qualified for safe harbor protection under the DMCA because the District Court applied too narrow a definition of 'repeat infringer'; 2) reversed as to judgment as a matter of law to the defendants on claims that defendant permitted infringement of plaintiffs' copyrights in pre‐2007 MP3s and Beatles songs because there was sufficient evidence to allow a reasonable jury to conclude that defendant had red‐flag knowledge of, or was willfully blind to, infringing activity involving those categories of protected material; 3) remanded for further proceedings related to claims arising out of the District Court’s grant of partial summary judgment; and 4) affirmed in all other respects.

#architektura #architekt #dom #design

Sorry all, rides over. See you in another life~

Hello, friends. We haven’t been releasing albums in quite a while, but we are still running our Discord server. We’ve been building a community for people who are interested in video game music, video games, and other assorted media, but are tired of the constant cynicism and negativity that permeate many communities with those interests. […]

Finding top quality MP3 moving download can be real assignment thinking about that moving songs is somewhat of a particular specialty showcase. Certain everybody appreciates clouding prone to the clubs and furthermore move, yet have you ever before in reality… Continue Reading →

An in-depth understanding of immune escape mechanisms in cancer is likely to lead to innovative advances in immunotherapeutic strategies. However, much remains unknown regarding these mechanisms and how they impact immunotherapy resistance. Using several preclinical tumor models as well as clinical specimens, we identified a mechanism whereby CD8+ T cell activation in response to programmed cell death 1 (PD-1) blockade induced a programmed death ligand 1/NOD-, LRR-, and pyrin domain–containing protein 3 (PD-L1/NLRP3) inflammasome signaling cascade that ultimately led to the recruitment of granulocytic myeloid-derived suppressor cells (PMN-MDSCs) into tumor tissues, thereby dampening the resulting antitumor immune response. The genetic and pharmacologic inhibition of NLRP3 suppressed PMN-MDSC tumor infiltration and significantly augmented the efficacy of anti–PD-1 antibody immunotherapy. This pathway therefore represents a tumor-intrinsic mechanism of adaptive resistance to anti–PD-1 checkpoint inhibitor immunotherapy and is a promising target for future translational research.

There is no description for this file!

There is no description for this file!

velocityconf: RT @OReillyAnimals Advice, please! Elephant Listening Project asks for ideas/solutions/help with 3 practical problems http://t.co/Y90YP3maoz

The transcription factor forkhead box P3 (FOXP3) is a biomarker for regulatory T cells and can also be expressed in cancer cells, but its function in cancer appears to be divergent. The role of hepatocyte-expressed FOXP3 in hepatocellular carcinoma (HCC) is unknown. Here, we collected tumor samples and clinical information from 115 HCC patients and used five human cancer cell lines. We examined FOXP3 mRNA sequences for mutations, used a luciferase assay to assess promoter activities of FOXP3's target genes, and employed mouse tumor models to confirm in vitro results. We detected mutations in the FKH domain of FOXP3 mRNAs in 33% of the HCC tumor tissues, but in none of the adjacent nontumor tissues. None of the mutations occurred at high frequency, indicating that they occurred randomly. Notably, the mutations were not detected in the corresponding regions of FOXP3 genomic DNA, and many of them resulted in amino acid substitutions in the FKH region, altering FOXP3's subcellular localization. FOXP3 delocalization from the nucleus to the cytoplasm caused loss of transcriptional regulation of its target genes, inactivated its tumor-inhibitory capability, and changed cellular responses to histone deacetylase (HDAC) inhibitors. More complex FKH mutations appeared to be associated with worse prognosis in HCC patients. We conclude that mutations in the FKH domain of FOXP3 mRNA frequently occur in HCC and that these mutations are caused by errors in transcription and are not derived from genomic DNA mutations. Our results suggest that transcriptional mutagenesis of FOXP3 plays a role in HCC.

Get the latest cybersecurity news, opinion and advice.

The transcription factor forkhead box P3 (FOXP3) is a biomarker for regulatory T cells and can also be expressed in cancer cells, but its function in cancer appears to be divergent. The role of hepatocyte-expressed FOXP3 in hepatocellular carcinoma (HCC) is unknown. Here, we collected tumor samples and clinical information from 115 HCC patients and used five human cancer cell lines. We examined FOXP3 mRNA sequences for mutations, used a luciferase assay to assess promoter activities of FOXP3's target genes, and employed mouse tumor models to confirm in vitro results. We detected mutations in the FKH domain of FOXP3 mRNAs in 33% of the HCC tumor tissues, but in none of the adjacent nontumor tissues. None of the mutations occurred at high frequency, indicating that they occurred randomly. Notably, the mutations were not detected in the corresponding regions of FOXP3 genomic DNA, and many of them resulted in amino acid substitutions in the FKH region, altering FOXP3's subcellular localization. FOXP3 delocalization from the nucleus to the cytoplasm caused loss of transcriptional regulation of its target genes, inactivated its tumor-inhibitory capability, and changed cellular responses to histone deacetylase (HDAC) inhibitors. More complex FKH mutations appeared to be associated with worse prognosis in HCC patients. We conclude that mutations in the FKH domain of FOXP3 mRNA frequently occur in HCC and that these mutations are caused by errors in transcription and are not derived from genomic DNA mutations. Our results suggest that transcriptional mutagenesis of FOXP3 plays a role in HCC.

Islet transplantation is an emerging therapy for type 1 diabetes (T1D) and hypoglycaemic unawareness. However, a key challenge for islet transplantation is cellular rejection and the requirement for long-term immunosuppression. In this study we established a diabetic-humanized NOD-scidIL2R^null(NSG) mouse model of T cell mediated human islet-allograft rejection and developed a therapeutic regimen of low-dose recombinant human interleukin2(IL-2) combined with low-dose rapamycin to prolong graft survival. NSG-mice that had received renal-subcapsular human islet-allografts and were transfused with 1x10⁷ of human-spleen-mononuclear-cells (hSPMCs), reconstituted human CD45⁺ cells that were predominantly CD3⁺ T cells and rejected their grafts with a median survival time of 27 days. IL-2 alone (0.3x10⁶ IU/m² or 1x10⁶ IU/m²), or rapamycin alone (0.5-1mg/kg) for 3 weeks did not prolong survival. However, the combination of rapamycin with IL-2 for 3 weeks significantly prolonged human islet-allograft survival. Graft survival was associated with expansion of CD4⁺CD25⁺FOXP3⁺ Tregs and enhanced TGF-β production by CD4⁺ T cells. CD8⁺ T cells showed reduced IFN- production and reduced expression of perforin-1. The combination of IL-2 and rapamycin has the potential to inhibit human islet-allograft rejection by expanding CD4⁺FOXP3⁺ Tregs in vivo and supressing effector cell function, and could be the basis of effective tolerance-based regimens.

The transcription factor forkhead box P3 (FOXP3) is a biomarker for regulatory T cells and can also be expressed in cancer cells, but its function in cancer appears to be divergent. The role of hepatocyte-expressed FOXP3 in hepatocellular carcinoma (HCC) is unknown. Here, we collected tumor samples and clinical information from 115 HCC patients and used five human cancer cell lines. We examined FOXP3 mRNA sequences for mutations, used a luciferase assay to assess promoter activities of FOXP3's target genes, and employed mouse tumor models to confirm in vitro results. We detected mutations in the FKH domain of FOXP3 mRNAs in 33% of the HCC tumor tissues, but in none of the adjacent nontumor tissues. None of the mutations occurred at high frequency, indicating that they occurred randomly. Notably, the mutations were not detected in the corresponding regions of FOXP3 genomic DNA, and many of them resulted in amino acid substitutions in the FKH region, altering FOXP3's subcellular localization. FOXP3 delocalization from the nucleus to the cytoplasm caused loss of transcriptional regulation of its target genes, inactivated its tumor-inhibitory capability, and changed cellular responses to histone deacetylase (HDAC) inhibitors. More complex FKH mutations appeared to be associated with worse prognosis in HCC patients. We conclude that mutations in the FKH domain of FOXP3 mRNA frequently occur in HCC and that these mutations are caused by errors in transcription and are not derived from genomic DNA mutations. Our results suggest that transcriptional mutagenesis of FOXP3 plays a role in HCC.

Stories: 1) Becoming Mindful Of Medical Decision Making 2) Love Longitude? 'Maphead' Locates Geography Buffs 3) Roger Ebert: A Critic Reflects On 'Life Itself'

Stories: 1) Brad Pitt: 'Moneyball,' Life And 'The Stalkerazzi' 2) In 'Arabia,' Writing Life As You Wish You'd Lived It

Stories: 1) 'Moneyball': Tracking Down How Stats Win Games 2) 'Moneyball': A 'Bad News Bears' For MBAs 3) Some Familiar Faces Return To Fall TV Lineup

Stories: 1) The Greedy Battle For Iraq's 'Hearts And Minds' 2) The Bangles Are Back, And Still Clever As Ever

Story: As many as 12 million computers worldwide have been infected with a highly encrypted computer worm called Conficker. Writer Mark Bowden details how Conficker was discovered, how it works, and the ongoing programming battle to bring down Conficker in his book Worm: The First Digital World War.

Stories: 1) Gordon-Levitt, Reiser Tackle '50/50' Odds 2) Low Cut Connie: Contagious, Low-Brow Fun

Stories: 1) Deadly Insurgents With Ties To U.S. Dollars 2) The Trouble With Health Problems Near Gas Fracking

Stories: 1) 'Freedom': Franzen's Novel Earns High Praise 2) Franzen Tackles Suburban Parenting In 'Freedom' 3) An Atmospheric 'Shelter' For Era Full Of Foreboding 4) Want Good TV? Try These Three Shows

Story: Several apostles affiliated with the movement helped organize or spoke at Rick Perry's recent prayer rally. A leading apostle, C. Peter Wagner, talks about the movement and its missions, which include acquiring leadership positions in government, the media, and arts and entertainment.

Stories: 1) How The Financial Crisis Created A 'New Third World' 2) Unlike Most Marxist Jargon, 'Class Warfare' Persists

Nestin, an intermediate filament protein widely used as a marker of neural progenitors, was recently found to be expressed transiently in developing cortical neurons in culture and in developing mouse cortex. In young cortical cultures, nestin regulates axonal growth cone morphology. In addition, nestin, which is known to bind the neuronal cdk5/p35 kinase, affects responses to axon guidance cues upstream of cdk5, specifically, to Sema3a. Changes in growth cone morphology require rearrangements of cytoskeletal networks, and changes in microtubules and actin filaments are well studied. In contrast, the roles of intermediate filament proteins in this process are poorly understood, even in cultured neurons. Here, we investigate the molecular mechanism by which nestin affects growth cone morphology and Sema3a sensitivity. We find that nestin selectively facilitates the phosphorylation of the lissencephaly-linked protein doublecortin (DCX) by cdk5/p35, but the phosphorylation of other cdk5 substrates is not affected by nestin. We uncover that this substrate selectivity is based on the ability of nestin to interact with DCX, but not with other cdk5 substrates. Nestin thus creates a selective scaffold for DCX with activated cdk5/p35. Last, we use cortical cultures derived from Dcx KO mice to show that the effects of nestin on growth cone morphology and on Sema3a sensitivity are DCX-dependent, thus suggesting a functional role for the DCX-nestin complex in neurons. We propose that nestin changes growth cone behavior by regulating the intracellular kinase signaling environment in developing neurons. The sex of animal subjects is unknown.

SIGNIFICANCE STATEMENT Nestin, an intermediate filament protein highly expressed in neural progenitors, was recently identified in developing neurons where it regulates growth cone morphology and responsiveness to the guidance cue Sema3a. Changes in growth cone morphology require rearrangements of cytoskeletal networks, but the roles of intermediate filaments in this process are poorly understood. We now report that nestin selectively facilitates phosphorylation of the lissencephaly-linked doublecortin (DCX) by cdk5/p35, but the phosphorylation of other cdk5 substrates is not affected. This substrate selectivity is based on preferential scaffolding of DCX, cdk5, and p35 by nestin. Additionally, we demonstrate a functional role for the DCX-nestin complex in neurons. We propose that nestin changes growth cone behavior by regulating intracellular kinase signaling in developing neurons.

Latest SOLIDWORKS press releases, news, technical alerts and announcements. These feeds are seen on...

CYP450 enzymes are involved in biotransformation of chloroquine (CQ), but the role of the different metabolism profiles of this drug has not been properly investigated in relation to P. vivax recurrences. To investigate the influence of CYPs genotypes associated with CQ-metabolism on early recurrence rates of P. vivax, a case-control study was carried out. Cases included patients presenting an early recurrence (CQ-recurrent), defined as recurrence during the first 28 days after initial infection, plasma concentrations of CQ plus desethylchloroquine (DCQ, the major CQ metabolite) higher than 100 ng/mL. A control (CQ-responsive) with no parasite recurrence over the follow-up was also included. CQ and DCQ plasma levels were measured on Day 28. CQ CYPs (CYP2C8, CYP3A4 and CYP3A5) genotypes were determined by real-time PCR. An ex vivo study was conducted to verify CQ and DCQ efficacy in P. vivax isolates. The frequency of alleles associated with normal and slow metabolism was similar between the cases and controls for CYP2C8 (OR=1.45, 95% CI=0.51-4.14, p=0.570), CYP3A4 (OR=2.38, 95% CI=0.92-6.19, p=0.105) and CYP3A5 (OR=4.17, 95% CI=0.79-22.04, p=1.038) genes. DCQ levels were higher than CQ, regardless of the genotype. Regarding the DCQ/CQ rate, there was no difference between groups or between those patients who had a normal or mutant genotype. DCQ and CQ showed similar efficacy ex vivo. CYPs genotypes had no influence on early recurrence rates. Similar efficacy of CQ and DCQ ex vivo could explain the absence of therapeutic failure, despite presence of alleles associated with slow metabolism.

Yes, there are still portable music players in 2019, and we don't just mean the iPod touch. The question is whether you need one, and if so, which is the best MP3 player to buy?

AAXA's P300 Neo Pico Projector offers easy packability, long battery life, and flexible connectivity, making it a top pick for frequent travelers.

In the world of analog design, IP3—the third order intercept point, is a known parameter that is used to measure the linearity in the radio frequency (RF) components. The extracted IP3 values are very essential to determine the operating power ...(read more)

Hi All,

I saw the cadence tutorial on measuring IIP3 with 3 tones test (Lets say I have a mixer in the test so two tones are entered in the RF port and one is the LO).

Now, I would like to verify if my receiver meets the bluetooth standard. In the standard it says to enter a signal at -64dBm and two additional signals (interference) 

at -39dBm each which placed one k (lets say k equals to one for the example) channels apart and the other 2k channels apart (so 3 signals enter the RF port). These signals cause an intermodulation product to fall

at the frequency of the desired signal. I would like to measure the IIP3 in this case. 

Now, I need to enter 4 tones and the IIP3 is measured (based on cadence tutorial) using sweep in the hb.

I do not want to sweep power since I need to enter exact power. I tried to use multi sinusoidal option in the port with exact power but it does not work.

How in general am I be able to check communication standard in this way using virtuoso and measure IIP3?

Can someone please help me?

Thanks in advance!