TASHKENT, 21 September 2015 – An OSCE-organized week-long training course for Uzbek law enforcement bodies on identifying and tracing illegitimate assets and the various instruments of asset forfeiture and recovery began today in Tashkent.

Experts from the Basel Institute of Governance will share international best practices with senior representatives in charge of international legal co-operation and the fight against corruption and money laundering within the General Prosecutor’s Office, the Ministry of Interior and other law enforcement bodies.

“The ability to launder ill-acquired assets outside of the jurisdiction of their country of origin is one of the major enablers of corrupt behaviours,” said Ambassador Gyorgy Szabo, Project Co-ordinator in Uzbekistan. “This is why the OSCE regards the identification and recovery of illegitimate assets, no matter where they might be hidden, as a crucial element of the fight against corruption.”

The OSCE Project Co-ordinator in Uzbekistan organized the training course jointly with the Swiss Agency for Development and Co-operation, the Basel Institute of Governance and the General Prosecutor’s Office of Uzbekistan.

During the course, participants will also learn about the mechanisms of international mutual legal assistance and how they can be used during anti-corruption investigations.

Related Stories

• OSCE supports roundtable discussion in Uzbekistan on a methodology for assessing money-laundering and terrorism-financing risks
• OSCE organizes Regional Conference on Horticultural Trade in Tashkent
• OSCE Project Co-ordinator lights up central Tashkent with 20 solar-powered street lamps

The three men had been in unmarked graves after they were killed in the Korean War.

Allocation represents second instalment of untied grants for 2024-25, and is meant for all eligible district panchayats, block panchayats and gram panchayats in Kerala

The premise was simple: Search out local artisans and makers to best complete the ellipsis-ended phrase, “When you love your body, you …” We knew A-list artists in the East Bay’s bustling, creative atmosphere would number in the hundreds, but desire and ambition won out over realistic aspirations that might have prevented action. Here then, […]

Cartagena, Colombia :: Logos Hope's volunteers share the message of hope for the future with elderly residents at a nursing home.

Fr. Stephen looks at the importance of understanding our relationship with God as involving all of who and what we are and not reducing our relationship to a set of ideas.

Join Michael in a discussion about how we often neglect our bodies, the physical and spiritual ramifications to ourselves and loved ones, how this is profoundly unspiritual, and what we need to think and do to treat our physical selves the way God intends us to.

Are our physical bodies evil? Are they good? Fr. John explores these questions in light of Christ's Transfiguration.

Fr. Apostolos shares from 1 Corinthians 3, reminding us that our bodies belong to the Lord, "The temple of God is holy, and that is what you are."

We talk a lot about our souls. But what about our bodies? Are they important? Are they saved?

Campaigners say it should be easier for plus-size walkers to enjoy the countryside.

Due to information technologies the problem we are facing today is not a lack of information but too much information. This phenomenon becomes very clear when we consider two figures that are often quoted: Knowledge is doubling in many fields (biology, medicine, computer science, ...) within some 6 years; yet information is doubling every 8 months! This implies that the same piece of information/knowledge is published a large number of times with small variations.

Just look at an arbitrary news item. If considered of some general interest reports of it will appear in all major newspapers, journals, electronic media, etc. This is also the problem with information portals that tie together a number of large databases.

It is our contention that we need methods to reduce the huge set of information concerning a particular topic to a number of pieces of information (let us call each such piece an "essay" in what follows) that present a good cross-section of potential points of view. We will explain why one essay is usually not enough, yet the problem of reducing a huge amount of contributions to a digestible number of essays is formidable, indeed is science fiction at the moment. We will argue in this paper that it is one of the important tasks of computer sciences to start tackling this problem, and we will show that in some special cases partial solutions are possible.

Mother reportedly pushed her daughters into a water tank before jumping in herself.

.

Totally new ingredients will include offerings in the Men's Health, Sports/Active Nutrition and Energy categories. Previously launched but new to SupplySide ingredients include offerings in Muscle Health, Energy, Mood and Sleep categories. All of these ingredients are supported by clinical science, and most are considered “beverage-friendly” as part of PLT's growing beverage ingredient portfolio.

In addition to typical rubber sports flooring, wood-look rubber flooring fulfills a variety of fitness facility needs such as basketball courts, group fitness spaces and functional training areas.

Children's Geographies; 12/01/2022
(AN 160715512); ISSN: 14733285
Academic Search Premier

A Russian man who had been drifting for two months in the Sea of ​​Okhotsk with the bodies of his relatives was rescued. His boat was discovered by the crew of the fishing vessel "Angel". When the fishermen found the boat, the man was conscious and was even able to shout a few words. The first phrase spoken by the survivor was: "Not enough strength." In response, the crew of the vessel that found the man, a Sakhalin resident, said that they had taken this information into account and promised to save him quickly.

The third complementary-determining regions of the heavy-chain (CDR3H) variable regions (VH) of some cattle antibodies are highly extended, consisting of 48 or more residues. These `ultralong' CDR3Hs form β-ribbon stalks that protrude from the surface of the antibody with a disulfide cross-linked knob region at their apex that dominates antigen interactions over the other CDR loops. The structure of the Fab fragment of a naturally paired bovine ultralong antibody (D08), identified by single B-cell sequencing, has been determined to 1.6 Å resolution. By swapping the D08 native light chain with that of an unrelated antigen-unknown ultralong antibody, it is shown that interactions between the CDR3s of the variable domains potentially affect the fine positioning of the ultralong CDR3H; however, comparison with other crystallo­graphic structures shows that crystalline packing is also a major contributor. It is concluded that, on balance, the exact positioning of ultralong CDR3H loops is most likely to be due to the constraints of crystal packing.

Rescue workers move a stretcher containing recovered remains at the site of the collapsed Champlain Towers South condo building in Surfside, Fla., on Monday. Rescuers have recovered three more bodies and officials say the demolition of the building will accelerate search efforts.; Credit: Lynne Sladky/AP

Miami-Dade County mayor Daniella Levine Cava said on Monday morning that three more victims have been recovered from the ruins of the collapsed condo tower in Surfside, Fl., bringing the total death toll so far to 27.

Cava added that the demolition of the rest of Champlain Towers South "was executed exactly as planned" the previous evening, and that it would now allow rescue teams to work on a section of the collapsed building that was previously inaccessible. She noted that 118 individuals remain unaccounted for.

"Truly, we could not continue without bringing this building down," Cava said. "The area closest to the building was not accessible, due to the enormous risk to the team of first responders, because of the instability of the building."

According to Florida Governor Ron DeSantis, the newly accessible section of rubble is also from a part of the building where many bedrooms were located, and may contain the remains of many victims. The building collapsed in the middle of the night.

Authorities had been concerned that an approaching tropical storm might topple the standing part of the building onto the section that had already collapsed. That would have been a massive setback in the search for victims and for clues to the cause of the disaster.

Tropical Storm Elsa now appears to be tracking further to the west, and is more likely to hit the west coast of Florida, rather than the site of the disaster. But officials at the National Weather Service say the storm's course still could change.

Cava acknowledged that demolishing people's homes "is a devastating decision" and said that "our teams are doing everything possible to help those who lost their home begin to rebuild."

She said that authorities are working with insurance companies and the Federal Emergency Management Agency to streamline claims and help those who've lost homes and property.

One animal rescue volunteer had gone to court to stop the demolition of the rest of the tower, asking the court to allow more time to rescue pets that might still be trapped inside. The judge denied the motion.

Cava said in her Monday briefing that Miami-Dade rescue teams had already gone through parts of the building that were still accessible, "searching in closets and under beds" to find missing pets.

This content is from Southern California Public Radio. View the original story at SCPR.org.

The current process for planetary protection policy development is inadequate to respond to increasingly complex solar system exploration missions, says a new report from the National Academies of Sciences, Engineering, and Medicine.

During HIV infection, the virus mutates too rapidly for the immune system to combat, but some people produce antibodies that can recognize the virus even two years after infection. With an eye towards developing a vaccine, in four related papers from multiple groups publishing September 8 in Cell and Immunity, researchers describe a multi-step method for "training" the immune system to produce these antibodies in genetically engineered mice.

read more

Global Manager Group releases an affordable ISO 14065 documentation kit, opening up the possibility of GHG verification. Organizations can obtain certification with this easy-to-use toolkit without having to pay costly consultation fees.

Our immune systems generate antibodies that neutralize and help eliminate foreign pathogens. These antibodies or proteins are generated by specific immune

Monoclonal antibodies (mAbs) have revolutionized targeted cancer therapies with unmatched specificity and potency. Since the first mAb approval in 1986, ad

Our immune systems generate antibodies that neutralize and help eliminate foreign pathogens. These antibodies or proteins are generated by specific immune

Our immune systems generate antibodies that neutralize and help eliminate foreign pathogens. These antibodies or proteins are generated by specific immune

22^nd Global Standards Collaboration meeting convenes world’s leading standards bodies in Montreux, Switzerland

Sophia Antipolis, 28 March 2019

The 22nd meeting of the Global Standards Collaboration (GSC), a high-level gathering of the world’s leading information and communication technologies (ICT) standards organizations, took place from 26-27 March 2019, hosted by IEC (International Electrotechnical Commission) and ISO (International Organization for Standardization) in Montreux, Switzerland.  GSC members shared their priorities and focused on Smart Sustainable Cities and AI (Artificial Intelligence). 

Read More...

ETSI blockchain group releases first Reports, targeting industry and governmental bodies

Sophia Antipolis, 15 February 2021

The ETSI Industry Specification Group on Permissioned Distributed Ledger (ISG PDL) has recently released a number of Reports to support industry and government institutions needs for what is commonly known as blockchain. These Reports cover data record compliance to regulation, application scenarios and smart contracts.  

Read More...

Meet the eclectic J-pop trio, CHAMELEON LIME WHOOPIEPIE.

Learn more about sponsor message choices: podcastchoices.com/adchoices

NPR Privacy Policy

There’s power in a viral clapback. But what of the already-marginalized people whose identities again become a public punchline?

NEW YORK — The 2024 NAB Show New York concluded, solidifying its place as the must-attend East Coast event for not only broadcast, media and entertainment professionals but for anyone creating content across various industries. With more than 12,000 attendees, some 250 Exhibitors, 50 of which are first-timers, and 20 new product launches, the Show offered a comprehensive look at the latest trends and innovations shaping the industry. Held at the Jacob K. Javits Convention Center, the Show’s Q4 timing provided a pivotal opportunity for professionals to explore end-of-year purchases and prepare strategic decisions for 2025.

Room 29, Parliament Buildings

BELGRADE, 8 April 2016 - On the occasion of International Roma Day, the Delegation of the European Union to Serbia, the United Nations Country Team in Serbia, the OSCE Mission to Serbia and the Council of Europe Office in Belgrade reaffirm our strong commitment to equality and non-discrimination.

Today, the Romani community, Europe's largest ethnic minority, still experiences multiple discrimination and marginalisation in countries throughout Europe, including Serbia.  Roma remain one of the most vulnerable groups; they continue to face difficult living conditions and discrimination in access to social protection, health, employment and adequate housing, as stated in reports by both relevant international mechanisms and independent bodies of the Republic of Serbia.

Thousands of Roma represent the most vulnerable and marginalised among the population of internally displaced, still facing obstacles in accessing rights and durable solutions.

International and European human rights standards clearly provide for equality before the law and prohibit discrimination on all grounds, including ethnicity. The realisation of Roma inclusion will reinforce everyday democracy and the rule of law. It is the only way to reach a society based on the principle of equality of opportunity where everyone has the right to participate and to be heard.

On this day, we therefore call on all relevant stakeholders to pursue with determination 
Roma inclusion policies in Serbia, pursuant to the recent adoption of a new Strategy of Social Inclusion of Roma for the Period from 2015-2025 and the Action Plan for Realization of Rights of National Minorities by the Government of Serbia.

The Delegation of the European Union, the Council of Europe Office in Belgrade, the OSCE Mission to Serbia and the United Nations Country Team in Serbia remain committed to improving the lives of Roma in Serbia and  ensuring that their fundamental rights are respected, and to supporting our Serbian partners in this endeavour.

International Roma Day is celebrated annually on April 8, in commemoration of the 1971 founding of the International Romani Union, the first major international Romani civic initiative.

Statistical information on Roma in Serbia

• 90% of children in Roma settlements are growing up in poverty. The infant and under-5 mortality rate is two times higher than that of the general population, 15% of Roma children are born with low birth weights, one fifth is malnourished and only 13% are fully immunized.
• Romani children continue to face discrimination in the education system. Only 6% of children from Roma settlements attend early learning programs, only 64% finish primary school on time, and  only 22% attend secondary school (of whom only 15% are girls).
• Romani women and girls face multi-sectoral discrimination, as well as heightened barriers of access to services, including services for victims of violence.    Early, arranged marriage remains an issue in some segments of Serbia’s Romani communities, with very destructive consequences for – in particular – the child bride.

For more information & media inquiries please contact:

Aleksandra Stamenkovic, United Nations Country Team Communications Expert, email: aleksandra.stamenkovic@one.un.org, Phone +381 11 415 5314

Nadezda Dramicanin, Delegation of the EU to Serbia Communications Officer, e-mail: Nadezda.DRAMICANIN@eeas.europa.eu, Phone: +381 11 3083 200

Ivana Milatovic, OSCE National Political and Press Affairs Officer, e-mail: ppiu-serbia@osce.org, Phone +381 11 3606 151

Mona Alghaith, Council of Europe Office, Assistant to the Head of Office, e-mail: mona.alghaith@coe.int, Phone. +381 11 71 555 00

Related Stories

• EU Delegation, OSCE Mission, closely following situation in Serbia’s public service broadcasters
• We Are Here Together - European Support to Roma Inclusion
• OSCE Mission to Serbia, ODIHR and civil society partners to follow election of judges to High Judicial Council

A memorandum of understanding signed on 19 May 2016 by the OSCE Office in Yerevan, the Ministry of Territorial Administration and Development, and the Union of Government Employees NGO aims to enhance the transparency and accountability of the community civil service of Armenia’s municipalities, local self-government bodies, and to improve relevant legislation, bringing it in line with international standards to reduce corruption in public administration.

Initiatives will include professional and regular training on principles and international standards of integrity in civil service, corruption risks, detection and prevention of corruption, reforms to training and recruitment of community servants in the regions, identifying legislation containing corruption risks, and recommendations for systemic reform.

“We hope this joint endeavour will contribute to the government’s efforts in implementing the anti-corruption strategy and in promoting efficient and accountable governance in Armenia,” said the Head of the OSCE Office in Yerevan Ambassador Argo Avakov.

David Lokyan, Armenia’s Minister of Territorial Administration and Development underlined the importance of co-operation in promoting better civil and community service, integrity and accountability among the local authorities, legislation reform in ensuring high-quality public administration in line with international standards throughout the regions in Armenia.

The initiative builds on Office-supported activities to train civil servants; improve the civil and community service system, including recruitment, monitoring and attestation; and to mainstream integrity in the public administration. A series of training courses for civil servants were held by the Union of Armenia's Government Employees NGO with the support of the OSCE Office and in partnership with the Civil Service Council of Armenia.

Vache Kalashyan, Chairperson of the Union of Government Employees, said: "We enjoy continuous co-operation with the civil and community services sector and are happy to enhance knowledge of corruption risks, conflict of interest, integrity and strengthen civil servants’ impact in delivering accountable and high-quality services on behalf of the State.”

Between 2009 and 2015 more than 300 civil servants representing 35 different state services were trained with the OSCE Office’s support on integrity, anti-corruption and ethics. 

Related Stories

• Corruption issues in private sector discussed with support of the OSCE Office in Yerevan
• OSCE enhances legal and human rights education in Armenia
• OSCE Office in Yerevan presents awards to best women entrepreneurs in Armenia

BELGRADE, 8 April 2016 - On the occasion of International Roma Day, the Delegation of the European Union to Serbia, the United Nations Country Team in Serbia, the OSCE Mission to Serbia and the Council of Europe Office in Belgrade reaffirm our strong commitment to equality and non-discrimination.

Today, the Romani community, Europe's largest ethnic minority, still experiences multiple discrimination and marginalisation in countries throughout Europe, including Serbia.  Roma remain one of the most vulnerable groups; they continue to face difficult living conditions and discrimination in access to social protection, health, employment and adequate housing, as stated in reports by both relevant international mechanisms and independent bodies of the Republic of Serbia.

Thousands of Roma represent the most vulnerable and marginalised among the population of internally displaced, still facing obstacles in accessing rights and durable solutions.

International and European human rights standards clearly provide for equality before the law and prohibit discrimination on all grounds, including ethnicity. The realisation of Roma inclusion will reinforce everyday democracy and the rule of law. It is the only way to reach a society based on the principle of equality of opportunity where everyone has the right to participate and to be heard.

On this day, we therefore call on all relevant stakeholders to pursue with determination 
Roma inclusion policies in Serbia, pursuant to the recent adoption of a new Strategy of Social Inclusion of Roma for the Period from 2015-2025 and the Action Plan for Realization of Rights of National Minorities by the Government of Serbia.

The Delegation of the European Union, the Council of Europe Office in Belgrade, the OSCE Mission to Serbia and the United Nations Country Team in Serbia remain committed to improving the lives of Roma in Serbia and  ensuring that their fundamental rights are respected, and to supporting our Serbian partners in this endeavour.

International Roma Day is celebrated annually on April 8, in commemoration of the 1971 founding of the International Romani Union, the first major international Romani civic initiative.

Statistical information on Roma in Serbia

• 90% of children in Roma settlements are growing up in poverty. The infant and under-5 mortality rate is two times higher than that of the general population, 15% of Roma children are born with low birth weights, one fifth is malnourished and only 13% are fully immunized.
• Romani children continue to face discrimination in the education system. Only 6% of children from Roma settlements attend early learning programs, only 64% finish primary school on time, and  only 22% attend secondary school (of whom only 15% are girls).
• Romani women and girls face multi-sectoral discrimination, as well as heightened barriers of access to services, including services for victims of violence.    Early, arranged marriage remains an issue in some segments of Serbia’s Romani communities, with very destructive consequences for – in particular – the child bride.

For more information & media inquiries please contact:

Aleksandra Stamenkovic, United Nations Country Team Communications Expert, email: aleksandra.stamenkovic@one.un.org, Phone +381 11 415 5314

Nadezda Dramicanin, Delegation of the EU to Serbia Communications Officer, e-mail: Nadezda.DRAMICANIN@eeas.europa.eu, Phone: +381 11 3083 200

Ivana Milatovic, OSCE National Political and Press Affairs Officer, e-mail: ppiu-serbia@osce.org, Phone +381 11 3606 151

Mona Alghaith, Council of Europe Office, Assistant to the Head of Office, e-mail: mona.alghaith@coe.int, Phone. +381 11 71 555 00

Related Stories

• EU Delegation, OSCE Mission, closely following situation in Serbia’s public service broadcasters
• We Are Here Together - European Support to Roma Inclusion
• More than 1,000 local inspectors in Serbia successfully complete OSCE-supported online training course

If you’ve ever seen yourself through a thermal imaging camera, you’ll know that your body produces lots of heat. This is in fact a waste product of our metabolism. Every square foot of the human body gives off heat equivalent to about 19 matches per hour. Unfortunately, much of this heat simply escapes into the atmosphere. Wouldn’t it be great if we could harness it to produce energy? My research has shown this would indeed be possible. My colleagues and I are discovering ways of capturing and storing body heat for energy generation, using eco-friendly materials. The goal is to…

This story continues at The Next Web

The reports of multistakeholder Internet governance's demise are greatly exaggerated. This article explores the dual nature of multistakeholderism: its evolving, sometimes contentious practice as the "First Body," and its enduring principle of actor plurality as the "Second Body." Despite criticism and challenges, multistakeholderism remains crucial for a resilient, non-state-led Internet, underscoring the need to adapt and uphold its foundational pluralism.

Second set from Glaswegian electro duo, successfully expanding their debut’s palette.

1. Manipur on boil: 2 more bodies found, 6 missing  The Times of India
2. Additional paramilitary forces rushed to Manipur amid spike in ethnic violence  Hindustan Times
3. Three women, three children missing after Jiribam gunfight; Meiteis and Kukis hold bandhs in Manipur  The New Indian Express
4. Letters to The Editor — November 13, 2024  The Hindu
5. Centre rushes 20 more CAPF companies to Manipur after fresh violence  Deccan Herald

Reliable, specific polyclonal and monoclonal antibodies are important tools in research and medicine. However, the discovery of antibodies against their targets in their native forms is difficult. Here, we present a novel method for discovery of antibodies against membrane proteins in their native configuration in mammalian cells. The method involves the co-expression of an antibody library in a population of mammalian cells that express the target polypeptide within a natural membrane environment on the cell surface. Cells that secrete a single-chain fragment variable (scFv) that binds to the target membrane protein thereby become self-labeled, enabling enrichment and isolation by magnetic sorting and FRET-based flow sorting. Library sizes of up to 109 variants can be screened, thus allowing campaigns of naïve scFv libraries to be selected against membrane protein antigens in a Chinese hamster ovary cell system. We validate this method by screening a synthetic naïve human scFv library against Chinese hamster ovary cells expressing the oncogenic target epithelial cell adhesion molecule and identify a panel of three novel binders to this membrane protein, one with a dissociation constant (KD) as low as 0.8 nm. We further demonstrate that the identified antibodies have utility for killing epithelial cell adhesion molecule–positive cells when used as a targeting domain on chimeric antigen receptor T cells. Thus, we provide a new tool for identifying novel antibodies that act against membrane proteins, which could catalyze the discovery of new candidates for antibody-based therapies.

Xiao-Ting Wen
Dec 17, 2020; 0:RA120.002119v1-mcp.RA120.002119
Research

Some cases of chylomicronemia are caused by autoantibodies against glycosylphosphatidylinositol-anchored HDL binding protein 1 (GPIHBP1), an endothelial cell protein that shuttles LPL to the capillary lumen. GPIHBP1 autoantibodies prevent binding and transport of LPL by GPIHBP1, thereby disrupting the lipolytic processing of triglyceride-rich lipoproteins. Here, we review the "GPIHBP1 autoantibody syndrome" and summarize clinical and laboratory findings in 22 patients. All patients had GPIHBP1 autoantibodies and chylomicronemia, but we did not find a correlation between triglyceride levels and autoantibody levels. Many of the patients had a history of pancreatitis, and most had clinical and/or serological evidence of autoimmune disease. IgA autoantibodies were present in all patients, and IgG4 autoantibodies were present in 19 of 22 patients. Patients with GPIHBP1 autoantibodies had low plasma LPL levels, consistent with impaired delivery of LPL into capillaries. Plasma levels of GPIHBP1, measured with a monoclonal antibody–based ELISA, were very low in 17 patients, reflecting the inability of the ELISA to detect GPIHBP1 in the presence of autoantibodies (immunoassay interference). However, GPIHBP1 levels were very high in five patients, indicating little capacity of their autoantibodies to interfere with the ELISA. Recently, several GPIHBP1 autoantibody syndrome patients were treated successfully with rituximab, resulting in the disappearance of GPIHBP1 autoantibodies and normalization of both plasma triglyceride and LPL levels. The GPIHBP1 autoantibody syndrome should be considered in any patient with newly acquired and unexplained chylomicronemia.

Ankylosing Spondylitis (AS) is a common, inflammatory rheumatic disease, which primarily affects the axial skeleton and is associated with sacroiliitis, uveitis and enthesitis. Unlike other autoimmune rheumatic diseases, such as rheumatoid arthritis or systemic lupus erythematosus, autoantibodies have not yet been reported to be a feature of AS. We therefore wished to determine if plasma from patients with AS contained autoantibodies and if so, characterize and quantify this response in comparison to patients with Rheumatoid Arthritis (RA) and healthy controls. Two high-density nucleic acid programmable protein arrays expressing a total of 3498 proteins were screened with plasma from 25 patients with AS, 17 with RA and 25 healthy controls. Autoantigens identified were subjected to Ingenuity Pathway Analysis in order to determine patterns of signalling cascades or tissue origin. 44% of patients with Ankylosing Spondylitis demonstrated a broad autoantibody response, as compared to 33% of patients with RA and only 8% of healthy controls. Individuals with AS demonstrated autoantibody responses to shared autoantigens, and 60% of autoantigens identified in the AS cohort were restricted to that group. The AS patients autoantibody responses were targeted towards connective, skeletal and muscular tissue, unlike those of RA patients or healthy controls. Thus, patients with AS show evidence of systemic humoral autoimmunity and multispecific autoantibody production. Nucleic Acid Programmable Protein Arrays constitute a powerful tool to study autoimmune diseases.

Antibodies play essential roles in both diagnostics and therapeutics. Epitope mapping is essential to understand how an antibody works and to protect intellectual property. Given the millions of antibodies for which epitope information is lacking, there is a need for high-throughput epitope mapping. To address this, we developed a strategy, Antibody binding epitope Mapping (AbMap), by combining a phage displayed peptide library with next generation sequencing. Using AbMap, profiles of the peptides bound by 202 antibodies were determined in a single test, and linear epitopes were identified for >50% of the antibodies. Using spike protein (S1 and S2)-enriched antibodies from the convalescent serum of one COVID-19 patient as the input, both linear and conformational epitopes of spike protein specific antibodies were identified. We defined peptide-binding profile of an antibody as the Binding Capacity (BiC). Conceptually, the BiC could serve as a systematic and functional descriptor of any antibody. Requiring at least one order of magnitude less time and money to map linear epitopes than traditional technologies, AbMap allows for high-throughput epitope mapping and creates many possibilities.

To identify novel autoantibodies of Takayasu arteritis (TAK) using HuProt array-based approach. A two-phase approach was adopted. In Phase I, serum samples collected from 40 TAK patients, 15 autoimmune disease patients, and 20 healthy subjects were screened to identify TAK-specific autoantibodies using human protein (HuProt) arrays. In Phase II, the identified candidate autoantibodies were validated with TAK-focused arrays using an additional cohort comprised of 109 TAK patients, 110 autoimmune disease patients, and 96 healthy subjects. Subsequently, the TAK-specific autoantibodies validated in Phase II were further confirmed using Western blot analysis. We identified and validated eight autoantibodies as potential TAK-specific diagnostic biomarkers, including anti-SPATA7, -QDPR, -SLC25A2, -PRH2, -DIXDC1, -IL17RB, -ZFAND4, and -NOLC1 antibodies, with AUC of 0.803, 0.801, 0.780, 0.696, 0.695, 0.678, 0.635 and 0.613, respectively. SPATA7 could distinguish TAK from healthy and disease controls with 73.4% sensitivity at 85.4% specificity, while QDPR showed 71.6% sensitivity at 86.4% specificity. SLC25A22 showed the highest sensitivity of 80.7%, but at lower specificity of 67.0%. In addition, PRH2, IL17RB and NOLC1 showed good specificities of 88.3%, 85.9% and 86.9%, respectively, but at lower sensitivities (<50%). Finally, DIXDC1 and ZFAND4 showed moderate performance as compared with the other autoantibodies. Using a decision tree model, we could reach a specificity of 94.2% with AUC of 0.843, a significantly improved performance as compared to that by each individual biomarker. The performance of three autoantibodies, namely anti-SPATA7, -QDPR and -PRH2, were successfully confirmed with Western blot analysis. Using this two-phase strategy, we identified and validated eight novel autoantibodies as TAK–specific biomarker candidates, three of which could be readily adopted in a clinical setting.

Bispecific antibodies (bsAbs) are engineered to target 2 different epitopes simultaneously. About 75% of the 16 clinically approved bsAbs have entered the clinic internationally since 2022. Hence, research on biomedical imaging of various radiolabeled bsAb scaffolds may serve to improve patient selection for bsAb therapy. Here, we provide a comprehensive overview of recent advances in radiolabeled bsAbs for imaging via PET or SPECT. We compare direct targeting and pretargeting approaches in preclinical and clinical studies in oncologic research. Furthermore, we show preclinical applications of imaging bsAbs in neurodegenerative diseases. Finally, we offer perspectives on the future directions of imaging bsAbs based on their challenges and opportunities.