The present invention provides assays for detecting and measuring the presence or level of anti-TNFα drug therapeutics and autoantibodies in a sample. The present invention is useful for optimizing therapy and monitoring patients receiving anti-TNFα drug therapeutics to detect the presence or level of autoantibodies (e.g., HACA and/or HAHA) against the drug.

In sporadic Alzheimer's disease, neurofibrillary lesion formation is preceded by extensive post-translational modification of the microtubule associated protein tau. Immunoassays have been developed recently that detect tau in biological specimens, thus providing a means for pre-mortem diagnosis of Alzheimer's disease, which has remained elusive. These assays have been improved by the analysis of relevant post-translational modifications, such as phosphorylation, however opportunity for improvement remains. The present invention addresses this issue by disclosing synthetic methylated peptides derived from the tau protein of paired helical filaments and non-diseased control brain. Alzheimer's disease specificity is provided by the presence or absence of methyl moieties on lysine residues and differences between mono-, di-, and tri-methylation. The methylated peptide is useful as an antigen and a binding partner for identifying compounds that interact with the peptide and the methylated tau protein, including antibodies that can distinguish non-diseased brain from that affected by Alzheimer's disease. The resulting antibodies are useful diagnostically and therapeutically. The compounds that specifically bind to methylated tau proteins are useful for eliminating abnormally methylated tau.

The deaths of a 70-year-old man and his son, aged 30, after their boat flipped on a dam west of Bundaberg on Friday evening has hit the community hard, a mayor says, as she urges families to "embrace each other" on Father's Day.

Patients who end up on ventilators often face a long, slow and traumatic period of recuperation, which often includes relearning how to swallow, speak, breathe and walk.

The remains of a 77-year-old man and a 68-year-old woman are found on a property destroyed in the bushfires which ripped through northern NSW earlier this week.

The bodies of two climbing partners who fell to their deaths from a remote cliff face in South Australia's Flinders Ranges were discovered with the help of an aerial drone, one of the men's friends has revealed.

The search for two people missing off the coast of South Australia's Eyre Peninsula ends tragically as the bodies of a 35-year-old man and his five-year-old daughter are spotted in waters after they were swept from rocks at Cape Carnot.

Emergency crews recover the bodies of an Adelaide man and his five-year-old daughter who were hit by a freak wave on South Australia's Eyre Peninsula.

The woman, 32, and man, 24, were reported missing in the Cairns area yesterday after they both failed to show up for work.

Researchers at Australia's first 'body farm' have observed that dead bodies move significantly when they decompose and believe it could be important in investigations.

Tumor-associated peptide–human leukocyte antigen complexes (pHLAs) represent the largest pool of cell surface–expressed cancer-specific epitopes, making them attractive targets for cancer therapies. Soluble bispecific molecules that incorporate an anti-CD3 effector function are being developed to redirect T cells against these targets using 2 different approaches. The first achieves pHLA recognition via affinity-enhanced versions of natural TCRs (e.g., immune-mobilizing monoclonal T cell receptors against cancer [ImmTAC] molecules), whereas the second harnesses an antibody-based format (TCR-mimic antibodies). For both classes of reagent, target specificity is vital, considering the vast universe of potential pHLA molecules that can be presented on healthy cells. Here, we made use of structural, biochemical, and computational approaches to investigate the molecular rules underpinning the reactivity patterns of pHLA-targeting bispecifics. We demonstrate that affinity-enhanced TCRs engage pHLA using a comparatively broad and balanced energetic footprint, with interactions distributed over several HLA and peptide side chains. As ImmTAC molecules, these TCRs also retained a greater degree of pHLA selectivity, with less off-target activity in cellular assays. Conversely, TCR-mimic antibodies tended to exhibit binding modes focused more toward hot spots on the HLA surface and exhibited a greater degree of crossreactivity. Our findings extend our understanding of the basic principles that underpin pHLA selectivity and exemplify a number of molecular approaches that can be used to probe the specificity of pHLA-targeting molecules, aiding the development of future reagents.

musicisart magazine 2017 Best Music: Slenderbodies

Los Angeles-based duo Slenderbodies create music that feels like your watching the ocean. Slenderbodies recently released their brand new project fabulist out now via Majestic Casual Records. The five-track collection is full of whispers of sensuality found within falsetto vocals accompanied with vibrations of soft guitars melted into funky and soulful bass-lines that collide together […]

The post 2017 Best Music: Slenderbodies appeared first on musicisart magazine.

Disturbing videos posted online paint a grim picture on how hospitals seem to be struggling to deal with bodies as they battle against the wave of coronavirus patients.

Blood centers are ramping up efforts to collect plasma from people who recovered from COVID-19 in hopes their coronavirus antibodies could save lives.

On the latest episode of "Prime Time," CNN's Chris Cuomo reveals he has recovered from COVID-19 and possesses antibodies. But what does that mean?

The al-Hota gorge became "a place of horror" under the jihadist group, Human Rights Watch says.

• Warm Bodies review
• The History of Zombie Movies That Rudy Likes
• What We Watched: Bob's Burgers, Some Bob Marley documentary, Movie 43 and The Innkeepers

Proteins that bind carbohydrate structures can serve as tools to quantify or localize specific glycans in biological specimens. Such proteins, including lectins and glycan-binding antibodies, are particularly valuable if accurate information is available about the glycans that a protein binds. Glycan arrays have been transformational for uncovering rich information about the nuances and complexities of glycan-binding specificity. A challenge, however, has been the analysis of the data. Because protein-glycan interactions are so complex, simplistic modes of analyzing the data and describing glycan-binding specificities have proven inadequate in many cases. This review surveys the methods for handling high-content data on protein-glycan interactions. We contrast the approaches that have been demonstrated and provide an overview of the resources that are available. We also give an outlook on the promising experimental technologies for generating new insights into protein-glycan interactions, as well as a perspective on the limitations that currently face the field.

Ankylosing Spondylitis (AS) is a common, inflammatory rheumatic disease, which primarily affects the axial skeleton and is associated with sacroiliitis, uveitis and enthesitis. Unlike other autoimmune rheumatic diseases, such as rheumatoid arthritis or systemic lupus erythematosus, autoantibodies have not yet been reported to be a feature of AS. We therefore wished to determine if plasma from patients with AS contained autoantibodies and if so, characterize and quantify this response in comparison to patients with Rheumatoid Arthritis (RA) and healthy controls. Two high-density nucleic acid programmable protein arrays expressing a total of 3498 proteins were screened with plasma from 25 patients with AS, 17 with RA and 25 healthy controls. Autoantigens identified were subjected to Ingenuity Pathway Analysis in order to determine patterns of signalling cascades or tissue origin. 44% of patients with Ankylosing Spondylitis demonstrated a broad autoantibody response, as compared to 33% of patients with RA and only 8% of healthy controls. Individuals with AS demonstrated autoantibody responses to shared autoantigens, and 60% of autoantigens identified in the AS cohort were restricted to that group. The AS patients autoantibody responses were targeted towards connective, skeletal and muscular tissue, unlike those of RA patients or healthy controls. Thus, patients with AS show evidence of systemic humoral autoimmunity and multispecific autoantibody production. Nucleic Acid Programmable Protein Arrays constitute a powerful tool to study autoimmune diseases.

Catherine Pihoker
Dec 1, 2005; 54:S52-S61
Section II: Type 1-Related Forms of Diabetes

Sisters -- Fiction.

Expelled by animals as a waste product, the gas appears to play a crucial role in keeping these bizarre, burrowing rodents safe

Colloidal gold–antibody conjugates are easy to prepare and are an excellent choice for microscopic applications. Colloidal gold is an aqueous suspension of nanometer-sized particles of gold. Typically, chloroauric acid, HAuCl₄, is reduced with dilute solutions of sodium citrate, as described here. This will cause the gold to form small aggregates that will associate with proteins. Gold particles of specific sizes can be isolated and differentiated microscopically, allowing these particles to be used for multiple-label experiments. Colloidal gold-labeled antibodies are widely used in electron microscopy (EM), and can be used for light microscopy but require additional steps (silver enhancement).

There are many uses for antibodies labeled with metal ions. Most of these methods involve first attaching a metal chelator to the antibody molecule. This is achieved using standard cross-linking chemistry and then adding the desired metal at appropriate concentration and pH. The method described here outlines a basic procedure for creating a lanthanide conjugate. Lanthanide conjugates are used for proximity assays, as MRI contrast agents, or for mass cytometry experiments. Different metals and chelators can be substituted, but the basic procedures are similar.

1. Bharat Biotech to lead human monoclonal antibodies project  The Hindu
2. Bharat Biotech leads CSIR project to develop antibodies against Covid-19  Times of India
3. Bharat Biotech To Lead CSIR’s Project To Develop Human Monoclonal Antibodies For Covid-19 Therapy  Swarajya
4. Bharat Biotech to develop human antibodies for COVID-19 therapy  Telangana Today
5. Bharat Biotech vows human monoclonal antibodies to neutralise COVID-19 in 6 months  Mumbai Mirror
6. View Full coverage on Google News

Perforation in the setting of retained esophageal foreign body is rare, but can be catastrophic. The role of imaging in screening for injury after removal has not previously been studied.

The rate of esophageal injury among children with retained esophageal foreign body is 1.3%. Intraoperative findings suggestive of injury are predictive of perforation. Routine chest radiography is not warranted in those who do not meet this criterion. (Read the full article)

They had started their journey on foot from Maharashtra hoping to reach Madhya Pradesh, but it was their bodies that reached their home districts of Shahdol and Umaria by special trains on Saturday...

WILMINGTON, Del. –  Governor John Carney on Friday authorized all meetings of public bodies to be conducted electronically until further notice. Governor Carney on Thursday issued a State of Emergency declaration to prepare for the spread of Coronavirus (COVID-19). The emergency declaration allows the State of Delaware executive branch agencies to conduct public meetings electronically to […]

A coalition of UK associations at the university level, and a few support organizations, has urged the UK government, for improving the student visa system, in meeting its future goals. The goal is to get 600,000 global higher education students in the…

Terming this as one of the first known antibodies to neutralize SARS-CoV-2, Jason McLellan, associate professor of molecular biosciences at UT Austin and co-senior author, made a reference to the virus that causes COVID-19.

Therefore, an alternate therapeutic regimen for early deployment is critical, the vaccine-maker said in the statement.

Meanwhile, member of the Bhartiya Rail Sangharsh Samiti Shan Khan has demanded for special trains from Surat to Rajasthan, Madhya Pradesh, Jharkhand, Bihar and Uttar Pradesh for stranded labourers.

This project brings together National Centre for Cell Science (NCCS), Pune; Indian Institute of Technology, Indore, and Gurgaon-based PredOmix Technologies in a collaborative mode for a public health emergency.

1. Bharat Biotech to lead human monoclonal antibodies venture  Microbioz India
2. Bharat Biotech to lead monoclonal antibodies project for Covid-19 therapy  The Financial Express
3. Bharat Biotech leads CSIR project to develop antibodies against Covid-19  Times of India
4. CSIR approves project to develop human monoclonal antibodies that can neutralize COVID-19 in patients  Times Now
5. Bharat Biotech to lead project on monoclonal antibodies therapy for COVID-19  The New Indian Express
6. View Full coverage on Google News

New Delhi (Sputnik): As many as 12 people lost their lives and several others were hospitalised due to a hazardous styrene gas leak at a South Korean factory in the southern Indian state of Andhra Pradesh on Thursday. ......

PESHAWAR: The Pakistan People’s Party and the representatives of medical organisations of Khyber Pakhtunkhwa have demanded an end to the privatisation of healthcare facilities in the province and provision of automated coronavirus testing machines to medical staff. The demand was made...

Chinese scientists say they have identified two antibodies that could be candidates for a cocktail treatment for patients with different strains of the coronavirus.The antibodies were found to work together as a team to prevent the virus from latching onto a host cell, in a study led by Chinese Centre for Disease Control and Prevention director George Fu Gao with collaborators from across the country.They said that even a mutant strain was likely to be neutralised because the antibodies…

PESHAWAR: The Pakistan People’s Party and the representatives of medical organisations of Khyber Pakhtunkhwa have demanded an end to the privatisation of healthcare facilities in the province...

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HERAT: Afghanistan has recovered 18 bodies of migrants who were allegedly beaten and tortured before being forced into a river by Iranian border guards last week, a senior Afghan official said...

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