knowledge

Canadian Blood Services discovery research lab contributes to new knowledge on neutrophils

Canadian Blood Services discovery research lab contributes to new knowledge on neutrophils


Thursday, August 29, 2024 Abby Wolfe

Dr. Donald R. Branch’s work as a discovery scientist at Canadian Blood Services focuses on immunotherapy, seeking to further enhance understanding of the cellular and molecular mechanisms involved in autoimmune diseases. Based in Toronto, Dr. Branch’s laboratory develops models of rheumatoid arthritis, Alzheimer’s disease, immune thrombocytopenia (ITP) and multiple sclerosis in mice. These models are particularly useful in examining how cells affected by these conditions respond to current therapeutic agents like intravenous immunoglobulin – a blood product made from human plasma commonly known as IVIg – and investigating potential alternative therapeutics.  

Over the years, Dr. Branch’s lab research findings have included the discovery of a special reagent that makes identification of antibodies easier by “zapping" autoantibodies,answers around unexpected side effects of IVIg therapy, and insights around recipients’ responses to bone marrow transplantation. It has also earned him numerous awards and accolades, including four from AABB, the Association for the Advancement of Blood & Biotherapies.

Dr. Don Branch, Canadian Blood Services senior scientist

New knowledge about neutrophils

Recently, contributions from Dr. Branch’s lab helped a U.K.-based team of researchers discover new information about how neutrophil function and activity is regulated. Neutrophils are a type of white blood cell that are part of the body’s normal immune response. A person’s neutrophil levels may be affected by infections as well as chronic or acute illnesses. Patients who have very low neutrophil counts and/or persistent infections may receive a boost to their neutrophil levels via granulocyte transfusion therapy. However, in some diseases, overactivation of neutrophils can also create an inflammatory environment and harm healthy tissue.

The research has been published in the high-impact scientific journal, Nature. This publication has filled a gap in knowledge about the mechanisms involved in maintaining balance between neutrophils’ infection-fighting power and inflammation-causing potential. As Dr. Branch describes: “This research by Dr. Brown and team shows that the myeloid inhibitory C-type lectin enzyme, which is known as MICL, controls neutrophil activity in rheumatoid arthritis. It is likely that MICL also regulates neutrophil activity in general. This suggests that if therapeutics can be designed to target MICL, they may be useful in controlling inflammation and infection. It is a fundamental finding that will change our understanding of the biology of neutrophils.”  

Discovery research, like the study to which Don and Ruqayyah contributed, is essential to improving patient care in the long run. New biomedical insights are essential to crafting new drugs and new therapeutic approaches in a rational way.

Dr. William (Bill) Sheffield, Canadian Blood Services senior scientist and associate director of research

Discovery research expertise leads to new collaboration  

The special mouse model that Dr. Branch’s lab uses is known as K/BxN. In this model, two mouse populations are selectively bred to produce offspring whose genetic material carry an antibody that destroys bone cartilage. An arthritis-causing serum containing this destructive antibody can then be obtained from the mice for use as a reagent in research. This process is termed “serum-transfer arthritis” and has been used by many investigators in the study of treatments that may alleviate the condition. 

Knowledge of Dr. Branch’s lab’s expertise in this area prompted a connection to the team of researchers at the Universities of Exeter and Aberdeen in the U.K. for this study. Says Dr. Branch, “In 2021, I supported a successful application to Canadian Blood Services’ Blood Efficiency Accelerator Program (BEAP) by Dr. Maria Fernandes, a researcher from  Héma-Québec who works with neutrophils. Through this collaboration, Dr. Fernandes was aware that I work on rheumatoid arthritis with K/BxN mice, and she also knew that Dr. Gordon Brown in the U.K. was looking to collaborate with a researcher in this area for some planned neutrophil research. My lab, which included postdoctoral fellow Dr. Ruqayyah Almizraq at the time, contributed to Dr. Brown’s research by providing the sera from our K/BxN mice. With this sera, Dr. Brown and his collaborators were able to induce rheumatoid arthritis and show that it could be resolved by removing the inhibition of a specific enzyme.”  

Science that is never boring

The Branch lab is currently using these same mouse models to investigate whether a recombinant protein called IgG1 Fc hexamer could be an effective replacement for IVIg in the treatment of autoimmune conditions like rheumatoid arthritis and ITP. While early results are promising, continued work is needed. In fact, back in 2018, Dr. Branch was asked in a previous R.E.D. blog post to describe what he found to be the most exciting part of science. Reflecting on the same question now, he reiterates that his response still rings true after 50 years in the field:   

The most exciting part of science is that when you do good science, you get more questions than you get answers, which means it is never boring. There is no end to imagination; new ideas, theories, experiments and discoveries occur often and provide a level of excitement to which many other professions cannot attest.

Dr. Donald (Don) Branch, Canadian Blood Services senior scientist


Canadian Blood Services – Driving world-class innovation 

Through discovery, development and applied research, Canadian Blood Services drives world-class innovation in blood transfusion, cellular therapy and transplantation—bringing clarity and insight to an increasingly complex healthcare future. Our dedicated research team and extended network of partners engage in exploratory and applied research to create new knowledge, inform and enhance best practices, contribute to the development of new services and technologies, and build capacity through training and collaboration. Find out more about our research impact.   

The opinions reflected in this post are those of the author and do not necessarily reflect the opinions of Canadian Blood Services nor do they reflect the views of Health Canada or any other funding agency. 

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If any of these questions interest you, there is good news: the latest version (v23.1) of the Physical Verification Rules Writer (PVLRW) course is designed to teach you rule deck writing. This free 16-hour online course includes audio and labs designed to make your learning experience comfortable and flexible. Whether you are new to the concept or an experienced CAD/PDK engineer, the course is structured to enhance your rule deck writing skills.

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Pegasus Input and Output

Pegasus is a cloud-ready physical verification signoff solution that enables engineers to support faster delivery of advanced-node integrated circuits (ICs) to market.

Pegasus requires input data in the form of layout geometry, schematic netlists, and rules that direct the tool operation. The rules fall into two categories: those that describe the fabrication process and those that control the job-specific operation.

Pegasus provides log and report files, netlists, databases, and error databases as output.

Overview of Pegasus Rule File

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The PVL rules are placed in a file that gets selected in a run from the GUI or the command line, as the user directs. PVL rules may be on separate lines within the file and can also be contained in named rule blocks.

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A layout view displaying the DRC violations

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A sample LVS Rule deck. 

TCL, Macros, and Conditional commands

Tcl is supported and used in various Pegasus functionalities, such as Pegasus rule files and Pegasus configurator. Macros are functional templates that are defined once and can be used multiple times in a rule file. Conditional Commands are used to process or skip specific commands in the rule file.

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  • On the Cadence Support portal, select Register Now and provide the requested information on the Registration page.
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  • If you need help with registration, contact support@cadence.com.

To stay up to date with the latest news and information about Cadence training and webinars, subscribe to the Cadence Training emails.

If you have questions about courses, schedules, online, public, or live onsite training, reach out to us at Cadence Training.

For any questions, general feedback, or future blog topic suggestions, please leave a comment.

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About Knowledge Booster Training Bytes

Knowledge Booster Training Bytes is an online journal that relays information about Cadence Training videos, online courses, and upcoming webinars in the Learning section of the Cadence Learning and Support portal. This blog category brings you direct links to these videos, courses, and other related material on a regular basis. Subscribe to receive email notifications about our latest Custom IC Design blog posts.




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Below are the definitions of the essential stages in the RTL2GDSII Flow. For further reference, these definitions are also accessible on YouTube.

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What are On-Chip Variations?  

     

Want to Learn More?

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