The ethical questions raised by emerging neuro-technologies

Shopping cart with payment gateway is a must-have functionality for an e-commerce website. The payment gateway allows the buyers to make payment online at the time of checkout. PayPal standard checkout is one of the easiest option to integrate payment gateway in the web application. A shopping cart helps users to select multiple products and PayPal allows users to checkout with credit/debit card payment. PHP shopping cart with PayPal makes e-commerce web applications more user-friendly and feature reach. In this tutorial, we will show you how to build shopping cart with payment gateway in PHP. This example shopping cart system

The post Shopping Cart with PayPal Payment Gateway in PHP appeared first on CodexWorld.

Location: Electronic Resource- 

Location: Electronic Resource- 

Medicaid can now pay for medical and mental health care delivered on the sidewalk. This will transform how care for unhoused people can be given in the states that take advantage of the policy change.

The CDC plans to knock on the doors of randomly selected homes in the Tijuana River Valley later this month to ask them about how the sewage crisis has affected their wellbeing.

The Justice Department will reportedly review the planned merger between the PGA Tour and the Saudi-backed LIV Golf.

Given the crush of bad news on our changing climate, choosing a 'green' career just might be a matter of survival.

The Microsoft founder reportedly transferred $1.8 billion worth of stocks to his estranged wife on the same day they announced the end of their 27-year marriage.

Many on Twitter call out the Microsoft co-founder and his estranged wife Melinda Gates shortly after they made public their split, but they tag the wrong Melinda.

In light of the couple's split announcement, it's revealed that the Microsoft founder took private getaways with his ex-girlfriend and fellow nerd techie Ann at a cozy beach cottage in North Carolina.

The Microsoft founder reportedly transferred $1.8 billion worth of stocks to his estranged wife on the same day they announced the end of their 27-year marriage.

Colgate-Palmolive India is experiencing growth in the expanding oral care market. The company reported a 10% rise in revenue. However, it recognizes market difficulties like fluctuating demand and rising costs. Colgate remains dedicated to improving oral health awareness in India. It aims to achieve this through education and product innovation. The company is focused on sustainability and social responsibility initiatives.

Environmental group Rainforest Action Network (RAN) alleges that palm oil derived from illegally cleared Indonesian wildlife reserves may have infiltrated the supply chains of major brands like Nestle and Procter & Gamble. RAN's investigation, supported by satellite imagery, points to the deforestation of Rawa Singkil Wildlife Reserve for palm oil plantations, potentially jeopardizing endangered species.

Loving your neighbor as yourself is the highest expression of God’s law.

Officials in Allentown, Pa., have released a roughly ten-minute surveillance video showing officers subduing and arresting a man in front of a local hospital on Monday evening. The man ends up face-down on the ground, and as two officers pin the man's arm behind his back, a third officer kneels on his neck. The release of the footage by Allentown police came days after activists tweeted a shorter, 26-second video , which has been viewed hundreds of thousand of times. Police say the man was taken into the hospital and, after treatment, was released. His name and medical details were not disclosed. Police also didn't release the names of the officers. Reaction to the video has sparked comparisons to what happened to George Floyd, the Black man who was killed by Minneapolis police on Memorial Day. Derek Chauvin, the white officer who was filmed kneeling on Floyd's neck for nearly nine minutes, has since been fired and faces a second-degree murder charge. Three other officers were also

Updated at 3:36 p.m. E.D.T. on December 4. President Trump may have been involved with a change to the Republican Party campaign platform last year that watered down support for U.S. assistance to Ukraine, according to new information from someone who was involved. Diana Denman, a Republican delegate who supported arming U.S. allies in Ukraine, has told people that Trump aide J.D. Gordon said at the Republican Convention in 2016 that Trump directed him to support weakening that position in the official platform. Ultimately, the softer position was adopted. Denman is scheduled to meet this week with the House and Senate Intelligence committees to discuss what she saw, said two sources familiar with the briefings. Investigators in Congress and elsewhere want to ask the San Antonio-area woman about how her proposal supporting Ukraine changed in the course of last year's convention. People familiar with the story described it to NPR. Robert N. Driscoll, a Washington-based lawyer for Denman

Today, we get an update on the 'Golden Gate 26'. Then, it's time again to celebrate all things pumpkin! And, a new musical shows how enslaved Black people sought freedom in Mexico.

Special guest John Moltz joins the show to discuss the various iPhone 15 “-gates”, rumors of Jony Ive working with OpenAI on an “AI device”, and more.

WITH current Australian laws making lawful surrogacy impossible, this Paddington couple are ‘over the moon’ after the birth of their twin sons in the US.

Kicking off in February.

Colorado’s election results tell us that more of our neighbors are feeling bad about Tuesday night’s presidential outcome than are feeling good, but what about the down-ballot races?

As U.S. Rep. Yadira Caraveo emphasizes abortion rights in her reelection race in Colorado's battleground 8th Congressional District, she's moved the right on immigration -- angering some allies -- while facing fierce attacks from supporters of Republican challenger Gabe Evans.

She wants to get us in formation.

On Saturday [May 7] a dedication event was held at the Bermuda Aquarium, Museum and Zoo to present Dr. David Wingate with a portrait, painted by local artist Henry Ward. A spokesperson said,  “The painting features Dr. Wingate assisting a fledging Cahow from its nest, with two Cahows in flight behind him. “The painting was […]

The MoonGate Group announced the arrival of Dr. Peter Chipeta on its medical team as specialist in internal medicine and general practice. A spokesperson said, “The MoonGate Group is pleased to announce the arrival of Dr. Peter Chipeta BSC, MD, MMED to the medical team as specialist in internal medicine and general practice. Dr. Chipeta’s […]

The police said they are “aware of a video clip currently circulating on social media purporting to show two persons engaging in sexual activity in public at Cup Match,” and said they are looking into the matter, and reminded the public “that offenses under the telecommunications act are committed even when only sharing material such […]

Oliver Betschart won the final round of the Gosling’s Moongate Tour at the Port Royal Golf Course after carding a 1-under par 70. Quinton Sherlock Jr, was second after shooting a 2-over 72, while Arriek Douglas who recently played on the Moonlight tour in Florida, along with Eric West, and Jarryd Dillas all finished T3rd […]

The Progressive Labour Party [PLP] is set to host its 59th Annual Delegates Conference on Wednesday [Oct 23], under the theme ‘The Power of One.’ A spokesperson said, “The event, which will be held at St. Paul A.M.E. Church, Centennial Hall, will run from 7:00 PM to 9:30 PM and promises an engaging lineup of […]

[Updated] The Progressive Labour Party [PLP] is holding their annual Delegates Conference this evening [Oct 23] at St. Paul A.M.E. Church, Centennial Hall, under the theme ‘The Power of One.’ PLP Party Chair Dawn Simmons previously said, “‘The Power of One’ reminds us that while individual contributions may seem small, together they create powerful change. […]

The series is set in a leafy Maryland suburb just outside of Washington, D.C., and in one the most affluent African American counties in the United States.

Quick, guess the lighting.

If you said two lights, and use of high-speed sync to get the shallow depth of field, that's a pretty good guess.

If you said zero lights and a rigid high-efficiency reflector, that's an even better (and correct) guess.

When working with an inexperienced assistant — or looking to introduce someone to the world of off-camera lighting — a rigid/high-efficiency reflector is a fantastic and inexpensive place to start.

The public can now step into the future of lunar exploration at Space Center Houston by entering an early mockup for Northrop Grumman's Gateway astronaut accommodations around the moon.

Everyone goes through moments of doubt. Moments when the question of “what am I doing with my life?” feels more like a heavy weight than a fleeting thought. These times, often called existential crises, are not just about feeling lost or confused—they can be pivotal moments for personal growth. For example, a common existential crisis ... Read more

The post How to Navigate an Existential Crisis and Find Meaning in Life appeared first on LifeHack.

Women's Ice Hockey vs Colgate

Passengers who bug airline staff by breaking boarding protocol will be met with an alarm

Biden campaign surrogate and actress Jane Fonda on Wednesday called the coronavirus “God’s gift to the left.” She described the coronavirus pandemic as an “existential crossroads” that can “help determine which way humanity” can go in addressing progressive issues like climate change. “What a great gift, what a tremendous opportunity, we are so lucky, we have […]

The post Biden Surrogate Jane Fonda Says The Coronavirus Is ‘God Gift To The Left’ appeared first on Hispolitica.

It’s time to hang your winter jacket in the closet and change your wardrobe to warmer clothes, because “Spring Is Coming” to the Avangate Affiliate Network. Our spring contest will make your sales bloom and may even fulfill your Amazon wishlist. (Feel free to think “beyond the wall” with creative promotions!)

Nonphotochemical quenching (NPQ) is a mechanism of regulating light harvesting that protects the photosynthetic apparatus from photodamage by dissipating excess absorbed excitation energy as heat. In higher plants, the major light-harvesting antenna complex (LHCII) of photosystem (PS) II is directly involved in NPQ. The aggregation of LHCII is proposed to be involved in quenching. However, the lack of success in isolating native LHCII aggregates has limited the direct interrogation of this process. The isolation of LHCII in its native state from thylakoid membranes has been problematic because of the use of detergent, which tends to dissociate loosely bound proteins, and the abundance of pigment–protein complexes (e.g. PSI and PSII) embedded in the photosynthetic membrane, which hinders the preparation of aggregated LHCII. Here, we used a novel purification method employing detergent and amphipols to entrap LHCII in its natural states. To enrich the photosynthetic membrane with the major LHCII, we used Arabidopsis thaliana plants lacking the PSII minor antenna complexes (NoM), treated with lincomycin to inhibit the synthesis of PSI and PSII core proteins. Using sucrose density gradients, we succeeded in isolating the trimeric and aggregated forms of LHCII antenna. Violaxanthin- and zeaxanthin-enriched complexes were investigated in dark-adapted, NPQ, and dark recovery states. Zeaxanthin-enriched antenna complexes showed the greatest amount of aggregated LHCII. Notably, the amount of aggregated LHCII decreased upon relaxation of NPQ. Employing this novel preparative method, we obtained a direct evidence for the role of in vivo LHCII aggregation in NPQ.

Staphylococcus aureus adhesion to the host's skin and mucosae enables asymptomatic colonization and the establishment of infection. This process is facilitated by cell wall-anchored adhesins that bind to host ligands. Therapeutics targeting this process could provide significant clinical benefits; however, the development of anti-adhesives requires an in-depth knowledge of adhesion-associated factors and an assay amenable to high-throughput applications. Here, we describe the development of a sensitive and robust whole cell assay to enable the large-scale profiling of S. aureus adhesion to host ligands. To validate the assay, and to gain insight into cellular factors contributing to adhesion, we profiled a sequence-defined S. aureus transposon mutant library, identifying mutants with attenuated adhesion to human-derived fibronectin, keratin, and fibrinogen. Our screening approach was validated by the identification of known adhesion-related proteins, such as the housekeeping sortase responsible for covalently linking adhesins to the cell wall. In addition, we also identified genetic loci that could represent undescribed anti-adhesive targets. To compare and contrast the genetic requirements of adhesion to each host ligand, we generated a S. aureus Genetic Adhesion Network, which identified a core gene set involved in adhesion to all three host ligands, and unique genetic signatures. In summary, this assay will enable high-throughput chemical screens to identify anti-adhesives and our findings provide insight into the target space of such an approach.

Proteins in the α-macroglobulin (αM) superfamily use thiol esters to form covalent conjugation products upon their proteolytic activation. αM protease inhibitors use theirs to conjugate proteases and preferentially react with primary amines (e.g. on lysine side chains), whereas those of αM complement components C3 and C4B have an increased hydroxyl reactivity that is conveyed by a conserved histidine residue and allows conjugation to cell surface glycans. Human α2-macroglobulin–like protein 1 (A2ML1) is a monomeric protease inhibitor but has the hydroxyl reactivity–conveying histidine residue. Here, we have investigated the role of hydroxyl reactivity in a protease inhibitor by comparing recombinant WT A2ML1 and the A2ML1 H1084N mutant in which this histidine is removed. Both of A2ML1s' thiol esters were reactive toward the amine substrate glycine, but only WT A2ML1 reacted with the hydroxyl substrate glycerol, demonstrating that His-1084 increases the hydroxyl reactivity of A2ML1's thiol ester. Although both A2ML1s conjugated and inhibited thermolysin, His-1084 was required for the conjugation and inhibition of acetylated thermolysin, which lacks primary amines. Using MS, we identified an ester bond formed between a thermolysin serine residue and the A2ML1 thiol ester. These results demonstrate that a histidine-enhanced hydroxyl reactivity can contribute to protease inhibition by an αM protein. His-1084 did not improve A2ML1's protease inhibition at pH 5, indicating that A2ML1's hydroxyl reactivity is not an adaption to its acidic epidermal environment.

Protein quality control is maintained by a number of integrated cellular pathways that monitor the folding and functionality of the cellular proteome. Defects in these pathways lead to the accumulation of misfolded or faulty proteins that may become insoluble and aggregate over time. Protein aggregates significantly contribute to the development of a number of human diseases such as amyotrophic lateral sclerosis, Huntington's disease, and Alzheimer's disease. In vitro, imaging-based, cellular studies have defined key biomolecular components that recognize and clear aggregates; however, no unifying method is available to quantify cellular aggregates, limiting our ability to reproducibly and accurately quantify these structures. Here we describe an ImageJ macro called AggreCount to identify and measure protein aggregates in cells. AggreCount is designed to be intuitive, easy to use, and customizable for different types of aggregates observed in cells. Minimal experience in coding is required to utilize the script. Based on a user-defined image, AggreCount will report a number of metrics: (i) total number of cellular aggregates, (ii) percentage of cells with aggregates, (iii) aggregates per cell, (iv) area of aggregates, and (v) localization of aggregates (cytosol, perinuclear, or nuclear). A data table of aggregate information on a per cell basis, as well as a summary table, is provided for further data analysis. We demonstrate the versatility of AggreCount by analyzing a number of different cellular aggregates including aggresomes, stress granules, and inclusion bodies caused by huntingtin polyglutamine expansion.

In SAS Merchandise Financial Planning, custom time frame-based data versions do not aggregate correctly when referenced in worksheets with standard hierarchy levels. The data does not aggregate correctly from l

Mutations of p53 protein occur in over half of all cancers, with profound effects on tumor biology. We present the first—to our knowledge—method for noninvasive visualization of p53 in tumor tissue in vivo, using SPECT, in 3 different models of cancer. Methods: Anti-p53 monoclonal antibodies were conjugated to the cell-penetrating transactivator of transcription (TAT) peptide and a metal ion chelator and then radiolabeled with ¹¹¹In to allow SPECT imaging. ¹¹¹In-anti-p53-TAT conjugates were retained longer in cells overexpressing p53-specific than non–p53-specific ¹¹¹In-mIgG (mouse IgG from murine plasma)-TAT controls, but not in null p53 cells. Results: In vivo SPECT imaging showed enhanced uptake of ¹¹¹In-anti-p53-TAT, versus ¹¹¹In-mIgG-TAT, in high-expression p53^R175H and medium-expression wild-type p53 but not in null p53 tumor xenografts. The results were confirmed in mice bearing genetically engineered KPC mouse–derived pancreatic ductal adenocarcinoma tumors. Imaging with ¹¹¹In-anti-p53-TAT was possible in KPC mice bearing spontaneous p53^R172H pancreatic ductal adenocarcinoma tumors. Conclusion: We demonstrate the feasibility of noninvasive in vivo molecular imaging of p53 in tumor tissue using a radiolabeled TAT-modified monoclonal antibody.

"Intuition helps us see the big picture," says filmmaker and sustainability leader Hrund Gunnsteinsdóttir. Reflecting on her work at the UN, she outlines three ways innsæi (the Icelandic word for "intuition") can help humanity face our greatest existential threats — urging us to use our inner wisdom as a guide for essential change.