Hear From Crypto Vets

Footage Foundation (Footage) invites the public and media to a dialogue event -- "Gender Equity through Dialogue" on Wednesday, 20th March from 12:30-2:00 pm at Church Center for the United Nations, Ground Floor, 777 United Nations Plaza, NY 10017.

West Virginia’s Intermediate Court of Appeals upheld the denial of a school worker’s claim of injury from a child tapping her on the head. Case: Hill v. Wyoming County Board of…

One of the main laws that applies to almost everything in our lives, including building digital products, is Murphy’s Law: “Anything that can go wrong will go wrong.” Our goal is to prevent things from going wrong and, if they do, mitigate the consequences. In this article, Victor Ponamarev explores different strategies for preventing users from making mistakes.

How did stars form 7 billion years ago, or approximately halfway between the Big Bang and now? This is what a recent study published in the Monthly Notices

How did stars form 7 billion years ago, or approximately halfway between the Big Bang and now? This is what a recent study published in the Monthly Notices

How did stars form 7 billion years ago, or approximately halfway between the Big Bang and now? This is what a recent study published in the Monthly Notices

More than 20 million face acute food insecurity.

Proteomics Analysis of Interactions between Drug-Resistant and Drug-Sensitive Cancer Cells: Comparative Studies of Monoculture and Coculture Cell Systems  ACS Publications

Multi-tiered chemical proteomic maps of tryptoline acrylamide–protein interactions in cancer cells  Nature.com

This episode takes a close look at transactions from different angles, starting with their fundamental properties of Atomicity, Consistency, Isolation, Durability but also investigating advanced topics like distributed or business transactions.

Dave Cross, owner of Magnum Solutions and author of GitHub Actions Essentials (Clapham Technical Press), speaks with SE Radio host Gavin Henry about GitHub actions, the value they provide, and the best practices for using them in your projects. Cross describes the vast range of things that developers can do with GitHub Actions, including some use cases you might never have thought about. They start with some general discussion of CI/CD and then consider the three main types of events that drive GitHub actions before digging in to details about fine-grained action events, Action Marketplace, contexts, yaml, docker base images, self-hosted runners, and more. They further explore identity management, permissions, dependency management, saving money, and how to keep your secrets secret.

Not all existing electrical installations lack energy efficiency, but most of them do. Especially if they were built 15-20 years ago and older. Although it is generally acknowledged that substantial energy savings are possible in most existing installations, the process... Read more

The post Five actions to achieve excellent energy savings in old electrical installations appeared first on EEP - Electrical Engineering Portal.

In many buildings, HVAC is the first or second item in terms of energy costs. This technical article deals with optimisation tips and energy efficiency savings in heating systems of a building. Heating systems have always been used when the... Read more

The post 8 actions to improve energy efficiency in heating systems appeared first on EEP - Electrical Engineering Portal.

129-24 : Self-certification: Amendments to the Rules of the Bourse de Montréal to define Riskless Transactions on Options

Orlando's theme parks and attractions dominate the nominations of 10Best travel website's reader poll.

Icon Park's new Play Pass includes admission to Wheel, Madame Tussauds, Sea Life, Museum of Illusions, more.

QSTR-SS7-DFS - SS7 vulnerabilities and mitigation measures for digital financial services transactions

FIGI - DFS - Technical Report on SS7 Vulnerabilities and mitigation measures for DFS transactions

FIGI - DFS - Technical Report on SS7 Vulnerabilities and mitigation measures for DFS transactions

Companies are beginning to apply traditional speech analytics to their automated conversations.

Thanks to this Palo Alto, Calif.-based provider of real-time speech understanding technology, agents have a choice in how they're heard and can proudly hold on to their unique voice characteristics.

[ENA] Addis Ababa, Ethiopia's vibrant capital, known as the "political capital of Africa," is positioned to enhance its standing as a premier destination for conference tourism. As the headquarters of the African Union (AU) and the United Nations Economic Commission for Africa (UNECA), the city has long been a focal point for international diplomacy. Now, with new developments in tourist infrastructure and culturally rich attractions, Addis Ababa is prepared to host even more international conferences, summits,

[This Day] Kayode Tokede

Robots can trick us into thinking we are socially interacting and slow our reactions, scientists say

Location: Electronic Resource- 

Location: Electronic Resource- 

Location: Electronic Resource- 

Location: Electronic Resource- 

Sustainability Through Optimising User Interactions Within The Transport Domain

There's starting off on the right foot, and then there's whatever happened Monday night inside Spokane Arena. The Gonzaga men's basketball team loudly announced itself as a national title contender late on college basketball's opening night with a dominating 101-63 win against Baylor…

Last week, Spokane Valley released details about how much the city’s multiple investigations into City Council member Al Merkel have cost. At the Nov. 5 Spokane Valley City Council meeting, an expense report on the agenda stated that two independent investigations found Merkel has displayed a pattern of intimidation and hostile interactions with the city’s workforce, and disregarded the Council’s Governance Manual and Washington state laws despite receiving extensive training on public records rules…

After being tapped to lead the Department of Government Efficiency with Vivek Ramaswamy, Elon Musk said all of the actions taken by the department will be posted online for "maximum transparency."

Copyright 2020 NPR. To see more, visit AILSA CHANG, HOST: President Trump made some incendiary comments today about race and policing. They came during a TV interview with CBS News. He was asked why African Americans are dying at the hands of police officers, and Trump angrily dismissed the question. He said more white people are killed by police. And then he spoke at a news event which was focused on China. That's one of his top foreign policy priorities. NPR's John Ruwitch has spent a lot of time reporting about China. Hey, John. JOHN RUWITCH, BYLINE: Good afternoon. CHANG: Good afternoon. But first, we're going to go and talk to your colleague, NPR White House correspondent Franco Ordoñez. Hey, Franco. FRANCO ORDOÑEZ, BYLINE: Hey. CHANG: Hey. So the president has been under pressure, obviously, to respond to all the emotion and outrage across the country after George Floyd was killed by police in Minneapolis. Tell us what President Trump said today. ORDOÑEZ: Well, he was asked about

How Election Day results on the federal, state, and local level impact the labor, arts, and LGBTQ+ communities. Finally, comedian W. Kamau Bell performs at the Mondavi Center at UC Davis.

Tuesday's election offered a wide range of results to digest, both in Colorado and nationally -- at times going in different directions. Here are major takeaways from voters' decisions.

Sometimes, I feel like eLearning drag/drop interactions get a bad rap. Maybe that's because Serious Instructional Designers seem fairly dismissive about them in casual conversation?...

Why do politics always make for the best meme material? We'll just let the politicians speak for themselves. 

In case you missed last night's democratic presidential debate, then we'll give you a little run down: Cory Booker accused Joe Biden of being high, Amy Klobuchar claimed she raised a crap-load of money from her ex-boyfriends, and Biden made an, uh, brain-dead comment about domestic violence. 

Scroll down to watch some of the highlights and view some of the internet's reactions!

June 22, 2022 (New York, NY) – Will Apple’s entry into the buy now, pay later (BNPL) space upend the already competitive industry? That’s the question many are trying to […]

Zhaonan Wang and Yingpu Deng
Math. Comp. 93 (), 2921-2942.
Abstract, references and article information

Nathan Brownlowe, Alcides Buss, Daniel Gonçalves, Jeremy B. Hume, Aidan Sims and Michael F. Whittaker
Trans. Amer. Math. Soc. 377 (), 5513-5560.
Abstract, references and article information

Sudip Ranjan Bhuia
Proc. Amer. Math. Soc. 152 (), 5249-5263.
Abstract, references and article information

High-throughput sequencing of hematologic malignancies and other cancers has revealed recurrent mis-sense mutations of genes encoding pre-mRNA splicing factors. The essential splicing factor U2AF2 recognizes a polypyrimidine-tract splice-site signal and initiates spliceosome assembly. Here, we investigate representative, acquired U2AF2 mutations, namely N196K or G301D amino acid substitutions associated with leukemia or solid tumors, respectively. We determined crystal structures of the wild-type (WT) compared with N196K- or G301D-substituted U2AF2 proteins, each bound to a prototypical AdML polypyrimidine tract, at 1.5, 1.4, or 1.7 Å resolutions. The N196K residue appears to stabilize the open conformation of U2AF2 with an inter-RNA recognition motif hydrogen bond, in agreement with an increased apparent RNA-binding affinity of the N196K-substituted protein. The G301D residue remains in a similar position as the WT residue, where unfavorable proximity to the RNA phosphodiester could explain the decreased RNA-binding affinity of the G301D-substituted protein. We found that expression of the G301D-substituted U2AF2 protein reduces splicing of a minigene transcript carrying prototypical splice sites. We further show that expression of either N196K- or G301D-substituted U2AF2 can subtly alter splicing of representative endogenous transcripts, despite the presence of endogenous, WT U2AF2 such as would be present in cancer cells. Altogether, our results demonstrate that acquired U2AF2 mutations such as N196K and G301D are capable of dysregulating gene expression for neoplastic transformation.

Replication protein A (RPA) is a eukaryotic ssDNA-binding protein and contains three subunits: RPA70, RPA32, and RPA14. Phosphorylation of the N-terminal region of the RPA32 subunit plays an essential role in DNA metabolism in processes such as replication and damage response. Phosphorylated RPA32 (pRPA32) binds to RPA70 and possibly regulates the transient RPA70-Bloom syndrome helicase (BLM) interaction to inhibit DNA resection. However, the structural details and determinants of the phosphorylated RPA32–RPA70 interaction are still unknown. In this study, we provide molecular details of the interaction between RPA70 and a mimic of phosphorylated RPA32 (pmRPA32) using fluorescence polarization and NMR analysis. We show that the N-terminal domain of RPA70 (RPA70N) specifically participates in pmRPA32 binding, whereas the unphosphorylated RPA32 does not bind to RPA70N. Our NMR data revealed that RPA70N binds pmRPA32 using a basic cleft region. We also show that at least 6 negatively charged residues of pmRPA32 are required for RPA70N binding. By introducing alanine mutations into hydrophobic positions of pmRPA32, we found potential points of contact between RPA70N and the N-terminal half of pmRPA32. We used this information to guide docking simulations that suggest the orientation of pmRPA32 in complex with RPA70N. Our study demonstrates detailed features of the domain-domain interaction between RPA70 and RPA32 upon phosphorylation. This result provides insight into how phosphorylation tunes transient bindings between RPA and its partners in DNA resection.

Julia Knöckel
Dec 22, 2020; 0:RA120.002432v1-mcp.RA120.002432
Research