A proposal to make civil partnerships available to different sex couples will strengthen equality and advance human rights in Scotland, according to a Holyrood Committee.

Others face prison on assault and resisting arrest charges Steven Snell, the Millsboro man who kidnapped and robbed an elderly Rehoboth Beach couple has been sentenced to prison. In July 2018, Snell, 30, went to a home on Cornwall Road asking to use the phone. Once inside, Snell threatened the couple with a box cutter, […]

Ishant Sharma travelled for 24 hours and arrived in New Zealand 72 hours before the start of the first Test at Wellington.

...

Police are investigating a house robbery in which a couple in Amanzimtoti, on the south coast of KwaZulu-Natal, believed to be in their 50s was stabbed. ......

A married couple from Maryland ages 85 and 86 were the victims of a deadly shooting at a veterans cemetery in Delaware.

They had visited more than 20 countries before coronavirus struck.

An analysis of the topics that cause arguments between newly-wed heterosexual couples puts a lack of affection at the top of the list, with little concern about who sleeps on which side of the bed

Title: What Couples Considering IVF Need to Know
Category: Health News
Created: 5/1/2019 12:00:00 AM
Last Editorial Review: 5/2/2019 12:00:00 AM

Title: Love in the Time of Coronavirus: Couples Feel the Strain of Lockdown
Category: Health News
Created: 4/28/2020 12:00:00 AM
Last Editorial Review: 4/29/2020 12:00:00 AM

In order to establish sustainable heat loading (heat removal and storage) in abandoned flooded mine workings it is important to understand the geomechanical impact of the cyclical heat loading caused by fluid injection and extraction. This is particularly important where significantly more thermal loading is planned than naturally occurs. A simple calculation shows that the sustainable geothermal heat flux from abandoned coal mines can provide less than a tenth of Scotland's annual domestic heating demand. Any heat removal greater than the natural heat flux will lead to heat mining unless heat storage options are also considered.

As a first step, a steady-state, fully saturated, 2D coupled hydromechanical model of a generalized section of pillar-and-stall workings has been created. Mine water rebound was modelled by increasing the hydrostatic pressure sequentially, in line with monitored mine water-level data from Midlothian, Scotland. The modelled uplift to water-level rise ratio of 1.4 mm m^–1 is of the same order of magnitude (1 mm m^–1) as that observed through interferometric synthetic aperture radar (InSAR) data in the coalfield due to mine water rebound. The modelled magnitude of shear stress at the pillar corners, as a result of horizontal and vertical displacement, is shown to increase linearly with water level. Mine heat systems are expected to cause smaller changes in pressure than those modelled but the results provide initial implications on the potential geomechanical impacts of mine water heat schemes which abstract or inject water and heat into pillar-and-stall coal mine workings.

Thematic collection: This article is part of the SJG Collection on Early-Career Research available at: https://www.lyellcollection.org/cc/SJG-early-career-research

Allostery exploits the conformational dynamics of enzymes by triggering a shift in population ensembles toward functionally distinct conformational or dynamic states. Allostery extensively regulates the activities of key enzymes within biosynthetic pathways to meet metabolic demand for their end products. Here, we have examined a critical enzyme, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (DAH7PS), at the gateway to aromatic amino acid biosynthesis in Mycobacterium tuberculosis, which shows extremely complex dynamic allostery: three distinct aromatic amino acids jointly communicate occupancy to the active site via subtle changes in dynamics, enabling exquisite fine-tuning of delivery of these essential metabolites. Furthermore, this allosteric mechanism is co-opted by pathway branchpoint enzyme chorismate mutase upon complex formation. In this study, using statistical coupling analysis, site-directed mutagenesis, isothermal calorimetry, small-angle X-ray scattering, and X-ray crystallography analyses, we have pinpointed a critical node within the complex dynamic communication network responsible for this sophisticated allosteric machinery. Through a facile Gly to Pro substitution, we have altered backbone dynamics, completely severing the allosteric signal yet remarkably, generating a nonallosteric enzyme that retains full catalytic activity. We also identified a second residue of prime importance to the inter-enzyme communication with chorismate mutase. Our results reveal that highly complex dynamic allostery is surprisingly vulnerable and provide further insights into the intimate link between catalysis and allostery.

Chalongrat Noree and Naraporn Sirinonthanawech

Previously, we have developed an extramitochondrial assembly system, where mitochondrial targeting signal (MTS) can be removed from a given mitochondrial enzyme, which could be used to characterize the regulatory factors involved in enzyme assembly/disassembly in vivo. Here, we demonstrate that addition of exogenous acetaldehyde can quickly induce the supramolecular assembly of MTS-deleted aldehyde dehydrogenase Ald4p in yeast cytoplasm. Also, by using PCR-based modification of the yeast genome, cytoplasmically targeted Ald4p cannot polymerize into long filaments when key functional amino acid residues are substituted, as shown by N192D, S269A, E290K and C324A mutations. This study has confirmed that extramitochondrial assembly could be a powerful external system for studying mitochondrial enzyme assembly, and its regulatory factors outside the mitochondria. In addition, we propose that mitochondrial enzyme assembly/disassembly is coupled to the regulation of a given mitochondrial enzyme activity.

Burkholderia sp. strain SG-MS1 and Pseudomonas sp. strain SG-MS2 have previously been found to mineralize (+)-pinoresinol through a common catabolic pathway. Here, we used comparative genomics, proteomics, protein semipurification, and heterologous expression to identify a flavoprotein from the vanillyl alcohol oxidase/p-cresol methyl hydroxylase (VAO/PCMH) enzyme family in SG-MS2 that carries out the initial hydroxylation of (+)-pinoresinol at the benzylic carbon. The cognate gene is translationally coupled with a downstream cytochrome gene, and the cytochrome is required for activity. The flavoprotein has a unique combination of cofactor binding and cytochrome requirements for the VAO/PCMH family. The heterologously expressed enzyme has a K_m of 1.17 μM for (+)-pinoresinol. The enzyme is overexpressed in strain SG-MS2 upon exposure to (+)-pinoresinol, along with 45 other proteins, 22 of which were found to be encoded by genes in an approximately 35.1-kb cluster also containing the flavoprotein and cytochrome genes. Homologs of 18 of these 22 genes, plus the flavoprotein and cytochrome genes, were also found in a 38.7-kb cluster in SG-MS1. The amino acid identities of four of the other proteins within the SG-MS2 cluster suggest they catalyze conversion of hydroxylated pinoresinol to protocatechuate and 2-methoxyhydroquinone. Nine other proteins upregulated in SG-MS2 on exposure to (+)-pinoresinol appear to be homologs of proteins known to comprise the protocatechuate and 2-methoxyhydroquinone catabolic pathways, but only three of the cognate genes lie within the cluster containing the flavoprotein and cytochrome genes.

IMPORTANCE (+)-Pinoresinol is an important plant defense compound, a major food lignan for humans and some other animals, and the model compound used to study degradation of the β-β' linkages in lignin. We report a gene cluster, in one strain each of Pseudomonas and Burkholderia, that is involved in the oxidative catabolism of (+)-pinoresinol. The flavoprotein component of the α-hydroxylase which heads the pathway belongs to the 4-phenol oxidizing (4PO) subgroup of the vanillyl alcohol oxidase/p-cresol methyl hydroxylase (VAO/PCMH) enzyme family but constitutes a novel combination of cofactor and electron acceptor properties for the family. It is translationally coupled with a cytochrome gene whose product is also required for activity. The work casts new light on the biology of (+)-pinoresinol and its transformation to other bioactive molecules. Potential applications of the findings include new options for deconstructing lignin into useful chemicals and the generation of new phytoestrogenic enterolactones from lignans.

To make the dream of settling down in a prosperous city come true, young couples, especially ones with limited budgets, need to prepare the right steps to get your home sweet home.

Getting married is one of life’s most memorable — and emotional — milestones in life. But with tight restrictions on gatherings due to COVID-19 pandemic, couples throughout Windsor and Essex County who had scheduled their weddings for this spring or summer have had little choice but to postpone their big day. “Weddings are an extremely […]

MP called for love to be added to list of permitted reasons for long-distance journeys

• Coronavirus – latest updates
• See all our coronavirus coverage

Couples separated by France’s strict coronavirus rules will remain lovelorn after ministers ruled out a proposed change to the law extending the country’s state of health emergency.

The “lovers’ amendment”, as it was called, was proposed by an MP during a debate on the legislation in the lower house the national assembly.

For the first time, researchers have succeeded in creating strong coupling between quantum systems over a greater distance. They accomplished this with a novel method in which a laser loop connects the systems, enabling nearly lossless exchange of information and strong interaction between them. The physicists reported that the new method opens up new possibilities in quantum networks and quantum sensor technology.

Follow our LIVE updates about the coronavirus outbreak here Coronavirus: The symptoms

A couple have been fined after getting stranded on a rock on the Scottish coast during the coronavirus lockdown.

A couple who were living their dream of sailing across the Atlantic have shared their "shock" of returning to land and hearing the news about the global coronavirus pandemic.

Flamingos form long-lasting friendships and some "behave like married couples", according to new research.

A couple spending their diamond wedding anniversary apart because of the coronavirus pandemic each got a special treat to make their celebrations a little sweeter thanks to their local supermarket.

Tributes have been paid to Ken and Betty Hill, who were together for more than 40 years

An Australian couple has discovered a novel way to meet up with neighbours and enjoy the good weather, despite the coronavirus lockdown.

A new biography of Harry and Meghan will "go beyond the headlines" and "reveal unknown details" of the couple's life together.

Auto-pay compounded Comcast error, leading to a year of $50 monthly overcharges.

'We've never been apart for sixty plus years, we don't do apart,' says Sidney Moore

Netflix's new dating series ended with a polarising finale, and one that also left a number of lingering questions about the show's two central couples

Cornwall was considered as a new location for the 2020 series

Has your wedding been affected by coronavirus? Deliveroo wants to help

Thanks to Zoom, 50 of their friends were able to attend.

For the first time, researchers have succeeded in creating strong coupling between quantum systems over a greater distance. They accomplished this with a novel method in which a laser loop connects the systems, enabling nearly lossless exchange of information and strong interaction between them. In the scientific journal Science, the physicists from the University of Basel and University of Hanover reported that the new method opens up new possibilities in quantum networks and quantum sensor technology.

Farmer John Lever and his wife Lillian say their Koorana Crocodile Farm has just two weeks of food left to feed 3,000 crocodiles, so they're offering an 'adopt a croc' program to raise funds.

Rod Peters is a real-life Darryl Kerrigan from movie The Castle — he has even extended his house to include uninterrupted views of the runway. And the noise? No problem at all.

James and Victoria Sylvester were refused a refund when their $10,000 holiday to Dubai was cancelled because of coronavirus. Now hundreds of consumers in similar situations are considering launching a class action.

A Fairfield, Calif., couple was indicted today by a federal grand jury in Sacramento, Calif., on federal civil rights charges related to an alleged bias-motivated assault on an Indian-American couple. The two-count indictment alleges that on the evening of July 14, 2007, Joseph and Georgia Silva committed a bias-motivated assault on another couple at a public beach in South Lake Tahoe, Calif.

The owner of two elder care homes in Long Beach, Calif., has pleaded guilty on March 23, 2009 to bringing undocumented aliens into the United States and forcing two of them to work at her businesses. Evelyn Pelayo, 53, a resident of Long Beach, pleaded guilty on March 23, 2009 to forced labor and unlawful conduct of holding passports to further forced labor.

Frederick Allen and Kimberlee Allen, both of Harwich, Mass., were arraigned today before Magistrate Judge Marianne B. Bowler in Boston on charges of conspiracy to defraud the United States, tax evasion and failure to file tax returns. The indictment alleges that the Allens conspired to defraud the United States of assessing and collecting federal income taxes from 1998 through April 2009.

Jefferson Calimlim Sr. and his wife, Elnora Calimlim, both medical doctors in Milwaukee, Wis., were each sentenced today to 72 months in prison for forcing a woman to work as their domestic servant and illegally harboring her for 19 years in their Brookfield, Wis., residence.

The United States has filed a lawsuit to bar a married couple’s alleged nationwide tax fraud scheme involving so-called asset protection.

Chief Judge Mary M. Lisi sentenced Albert Martin to 51 months in prison and Lorraine Martin to one year and one day in prison.

A federal grand jury in Atlanta has indicted an Ellenwood, Ga., husband and wife, Juna Gwendolyn Babb, 54, and Michael J. Babb, 53, on charges of conspiracy, forced labor, document servitude, which is confiscating someone’s passport and visa, and harboring an alien for financial gain, the Justice Department announced.

A federal grand jury has indicted a Ft. Worth, Texas, husband and wife, Emmanuel and Ngozi Nnaji, on charges of engaging in a nine-year scheme to compel the labor of a Nigerian victim as their domestic servant.

A federal jury has convicted an Arlington, Texas, husband and wife, Emmanuel and Ngozi Nnaji, of engaging in a nine-year scheme to compel the labor of a Nigerian victim as their domestic servant.

Gloria Chu and Ivan Chu of Taipei, Taiwan, each pleaded guilty to nine counts including conspiracy, false statements, and violations of both the Endangered Species Act and the Lacey Act.

Frederick Allen and Kimberlee Allen, both of Harwich, Mass., today were convicted of conspiracy, tax evasion and failure to file tax returns.