This ruling class of administrative state, big tech, corporations: all of these people think that they can get rid of Trump and we'll go back to normal. They're wedded to a broken system that has sold out the American people. And now they're going to try to sell out the American people and the middle class especially. They're not going to fix immigration; they're not going to fix trade deals; they're not going to break up big tech; they're not going to do any of these things. Continue reading →

1. Melania Trump's 'I am the most bullied person' video viral as she 'rejects' Jill Biden's tea invite  The Times of India
2. Melania Trump declines invite to meet Jill Biden over Mar-a-Lago raids when FBI ’snooped through her wardrobe’  Mint
3. Jimmy Kimmel again mocks Donald Trump for Melania's ‘interesting’ decision to…  Hindustan Times
4. Biden Invites Trump To White House, But Why Is Melania Refusing To Go?  News18
5. Melania Trump To Skip White House Meeting With Jill Biden: Report  NDTV

Look, we definitely don't want to hate on any particular candidate or take sides in this presidential election cycle, but Joe Biden has just been so meme-able this election season that we really had to take advantage of the material handed to us. We think that Biden supporters and haters alike will be able to laugh at these.

Poor ol' Joe Biden has been the subject of many memes in this election cycle. Whether you love him or hate him, you have to admit that they've been pretty amusing. This particular meme mocks a campaign avatar where one can insert their image next to text that says "I'm on team Joe!" It's moderately cringey to say the least, but cringey makes for the best meme material.

A look at the day ahead in European and global markets from Kevin Buckland

BANDAR SERI BEGAWAN, Brunei (Reuters) -- Vietnam Airlines will issue a request for proposal to aircraft manufacturers next year to purchase 50 narrowbody jets, its CEO said on Wednesday. The carrier last year signed a provisional deal with Boeing BA for 50 737 MAX planes that has yet to be…

An excellent post, one of the best things written this year in economics. Here is part of the bottom line: Inflation did make the median voter poorer during Biden’s term. In no part of the income distribution did wages grow faster while Biden was President than they did 2012-2020. This is true in…

Convenience store operator receives rival buyout offer from members of founding family

The following article, Pelosi Torches Kamala Harris and Joe Biden, was first published on The Black Sphere.

In issuing an inadvertent and unexpected backhand of Kamala Harris, former US House of Representatives Speaker Nancy Pelosi

Continue reading Pelosi Torches Kamala Harris and Joe Biden ...

The following article, Jill Biden Won’t Even Look at Kamala Harris, was first published on The Black Sphere.

I guess Kamala Harris didn't really own the joy of her campaign, but only rented it. Because the joy is gone. You can't even get a smirk out of Jill Biden these days...

Continue reading Jill Biden Won’t Even Look at Kamala Harris ...

Kamala Harris spent over a billion dollars on her campaign to succeed Joe Biden after he dropped out of the presidential race on July 21. She hobnobbed with famous celebrities, and spent millions on events aimed at reaching low-information voters. But millions of Americans didn’t realize Harris was even on the ballot prior to election […]

The post Millions of Americans didn’t know Harris had replaced Biden appeared first on Liberty Unyielding.

By David Blackmon In the wake of the election of President Donald Trump to serve a second term in office, along with presumptive Republican majorities in both houses of Congress, many are now asking about what the future will hold for the oddly named Inflation Reduction Act. Trump made it repeatedly clear on the campaign […]

The post Biden’s Signature Climate ‘Boondoggle’ Might Be On Chopping Block After Trump Win appeared first on Liberty Unyielding.

By Robert Schmad The Department of Health and Human Services (HHS) spends tens of millions of dollars annually on diversity-related expenses, according to a new report from the watchdog group Open The Books. American taxpayers foot a bill of roughly $38.7 million every year to employ 297 staffers conducting diversity, equity and inclusion (DEI) work […]

The post Biden-Harris Health Department Spent Tens Of Millions Annually On DEI appeared first on Liberty Unyielding.

By Harold Hutchison Former Acting Secretary of Homeland Security Chad Wolf told CNN host Jim Acosta Tuesday that the incoming Trump administration would “initiate a pretty large program” to locate children the Biden-Harris administration lost track of. At least 85,000 children placed into the custody of the Office of Refugee Resettlement (ORR) are unaccounted for, […]

The post Former Trump Official Reminds Jim Acosta Of ‘Over 330,000 Children’ Biden-Harris Admin Lost Track Of At Border appeared first on Liberty Unyielding.

As Chris Billam-Smith's unification bout against Gilberto Ramirez nears, BBC Sport speaks to those closest to him.

Democratic presidential nominee Joe Biden questioned President Trump’s response to commit to a peaceful transfer of power if he loses the Nov. 3 presidential election. When landed in Wilmington, Delaware Wednesday night after campaigning in North Carolina, Biden was asked about the president’s earlier remarks from his White House briefing. “What country are we in?” […]

The post Biden Slams Trump’s Election Comment: ‘What Country Are We In?’ appeared first on Hispolitica.

President Trump mocked Joe Biden Thursday evening for always calling a “lid” on campaign activities early in the morning, saying the Democratic presidential rival is “the lowest energy individual I’ve ever seen.” “Did you see [Biden] did a lid this morning again?” Trump told supporters during his campaign rally Thursday night at Cecil Airport hanger in Jacksonville, […]

The post Trump At Jacksonville Mocks Biden As ‘Lowest Energy Individual’ For Calling Campaign ‘Lid This Morning Again’ appeared first on Hispolitica.

A video surfaced on social media Friday showing the Democratic presidential nominee Joe Biden speaking to overseas U.S. service members where he called them “stupid bastards” and a “dull bunch.” During his last few months as Vice President, Biden in 2106 visited Al-Dhafra Air Base near the United Arab Emirates capital of Abu Dhabi and […]

The post Biden Calls U.S. Troops In 2016 ‘Stupid Bastards’ And A ‘Dull Bunch’ appeared first on Hispolitica.

The Trump campaign wants a third-party inspector to inspect both President Trump and Democratic presidential nominee Joe Biden ears for electronic devices or transmitters prior to Tuesday night’s presidential debate. Trump has consented to the inspection while the Biden campaign has declined. However, according to a source told Floridian Press that the Biden campaign agreed […]

The post Biden Campaign Rejects Trump Campaign Calls For Pre-Debate Inspection For Electronic Earpieces appeared first on Hispolitica.

President Trump fired back at Democratic presidential nominee Joe Biden intelligence after the former Vice President took a jab at his rival response to the coronavirus pandemic, stating the president needs “to get a lot smarter” or more people are going to die. The first of many sparring moments between the two candidates during the […]

The post Trump To Biden: ‘There’s Nothing Smart About You, Joe’ appeared first on Hispolitica.

Democratic nominee Joe Biden refused to answer the question during the first presidential debate Tuesday night as to whether he would support “packing” the Supreme Court if elected President and the Senate Republicans confirms President Trump’s nominee Amy Coney Barrett. “If Senate Republicans go ahead and confirm Justice Barrett there has been talk about ending the […]

The post Biden Refuses During Presidential Debate To Answer Court-Packing Question appeared first on Hispolitica.

Democratic presidential nominee Joe Biden said that President Trump does bear some responsibility for catching COVID-19 last week due to his refusal of not taking social distancing guidelines and not wearing a mask seriously. “Look, anybody who contracts the virus by essentially saying, ‘masks don’t matter; social distancing doesn’t matter’ — I think is responsible […]

The post Biden: Trump Is ‘Responsible’ For Contracting Coronavirus appeared first on Hispolitica.

Democratic presidential nominee Joe Biden is already looking to call off the second debate, saying if President Trump “still has COVID-19” come next week the two candidates shouldn’t face-off in Miami. “Well, uh, I think if [Trump] still has COVID, we shouldn’t have a debate,” Biden told reporters Tuesday evening in Maryland, according to a […]

The post Biden Says If Trump ‘Still Has COVID’ Call The Debate Off appeared first on Hispolitica.

Biden campaign surrogate and actress Jane Fonda on Wednesday called the coronavirus “God’s gift to the left.” She described the coronavirus pandemic as an “existential crossroads” that can “help determine which way humanity” can go in addressing progressive issues like climate change. “What a great gift, what a tremendous opportunity, we are so lucky, we have […]

The post Biden Surrogate Jane Fonda Says The Coronavirus Is ‘God Gift To The Left’ appeared first on Hispolitica.

The club's chairman jokes that it missed out on Hollywood investment "by just one vowel".

A military judge has canceled the Biden administration’s attempt to hand a plea deal to Khalid Sheikh Mohammed and two other terrorists who Biden wanted to give a lighter sentence. Biden Defense Secretary Lloyd Austin had tried to make a plea deal with the terrorists that ran against their previous sentences by allowing them to […]

The post Military Judge Cancels Biden’s Attempt to Give Aid to Terror Masterminds of 9-11 appeared first on The Lid.

The voters knew it instinctively on Tuesday, but the numbers also prove that Americans are worse off since Joe Biden became president. On Thursday, it was reported that real wages have declined since 2020 when Joe Biden took office. Per Zero Hedge: As Statista’s Felix Richter reports, according to exit polls, 46 percent of voters […]

The post Yes, America is Worse Off: Real Wages Declined in Biden Era appeared first on The Lid.

Joe Biden’s corrupt Federal Emergency Management Administration (FEMA) has been caught telling its on-the-ground operatives not to help anyone with a Donald Trump campaign sign in their yard. The news broke late last week when a whistleblower revealed agency messages that told workers to refuse to help Trump supporters in the wake of Hurricane Milton […]

The post Biden’s Corrupt FEMA Told Workers Not to Help Hurricane Victims Who Had Trump Signs appeared first on The Lid.

The American Civil Liberties Union wanted to keep “finding” more ballots for the election in Arizona ad infinitum, but the state’s Supreme Court finally denied their demands. The deadline for accepting more ballots in Arizona is already an absurd Nov. 10, five full days after Election Day. But the AZ ACLU wanted to keep miraculously […]

The post Court Denies Arizona ACLU’s Bid to Keep Counting More Ballots appeared first on The Lid.

Joe Biden’s stupid, corrupt regime can’t even get a simple Veterans Day message right. As the nation came together to celebrate those who served out country, Biden’s Department of Defense pushed out a social media message telling Americans “Veterans Day, honoring all who served.” While, that is a perfectly fine message, the DoD added a […]

The post Biden’s Nitwit Dept. of Defense Uses Photo of Chinese Jet in Veterans Day Message appeared first on The Lid.

I tested more than 30 bidets, from basic to luxe, to determine the most hygienic, comfortable, easy-to-install, and cost-effective models in 2024.

The following article, Biden’s Corrupt FEMA Told Workers Not to Help Hurricane Victims Who Had Trump Signs, was first published on Conservative Firing Line.

Joe Biden’s corrupt Federal Emergency Management Administration (FEMA) has been caught telling its on-the-ground operatives not to help anyone with a Donald Trump campaign sign in their yard. The news broke late last week when a whistleblower revealed agency messages that told workers to refuse to help Trump supporters in the wake of Hurricane Milton …

Continue reading Biden’s Corrupt FEMA Told Workers Not to Help Hurricane Victims Who Had Trump Signs ...

At the State of the Union, Joe Biden Joe Biden made a 'bold' announcement that America would be building a pier to deliver aid to Gaza. Four months later, he admitted it was a failure.

The post Soldier Succumbs To Injuries Sustained In Biden’s Doomed Gaza Pier Mission appeared first on Clash Daily.

There appeared to be a definite frost between first lady Jill Biden and Vice President Kamala Harris during a Veterans Day event at Arlington National Cemetery on Monday. A close-up […]

The post Footage of Jill Biden and Kamala Harris Sitting Side-by-Side at Veterans Day Event Goes Viral appeared first on The Western Journal.

Melania Trump will not be traveling with her husband to Washington on Wednesday when President-elect Donald Trump and President Joe Biden have a formal transition meeting, according to multiple reports. […]

The post Melania Trump to Give Jill Biden the Cold Shoulder This Week: Report appeared first on The Western Journal.

Legendary Christian writer C.S. Lewis gave us an analogy to help explain otherwise inexplicable moments such as this. Tolerance, Lewis once wrote, “parodies love as flippancy parodies merriment.” In a […]

The post Biden Asked If He'll Get a Hostage Deal by the End of His Term, and His Response Is Telling appeared first on The Western Journal.

Numerous iron-sulfur (Fe-S) proteins with diverse functions are present in the matrix and respiratory chain complexes of mitochondria. Although [4Fe-4S] clusters are the most common type of Fe-S cluster in mitochondria, the molecular mechanism of [4Fe-4S] cluster assembly and insertion into target proteins by the mitochondrial iron-sulfur cluster (ISC) maturation system is not well-understood. Here we report a detailed characterization of two late-acting Fe-S cluster-carrier proteins from Arabidopsis thaliana, NFU4 and NFU5. Yeast two-hybrid and bimolecular fluorescence complementation studies demonstrated interaction of both the NFU4 and NFU5 proteins with the ISCA class of Fe-S carrier proteins. Recombinant NFU4 and NFU5 were purified as apo-proteins after expression in Escherichia coli. In vitro Fe-S cluster reconstitution led to the insertion of one [4Fe-4S]2+ cluster per homodimer as determined by UV-visible absorption/CD, resonance Raman and EPR spectroscopy, and analytical studies. Cluster transfer reactions, monitored by UV-visible absorption and CD spectroscopy, showed that a [4Fe-4S]2+ cluster-bound ISCA1a/2 heterodimer is effective in transferring [4Fe-4S]2+ clusters to both NFU4 and NFU5 with negligible back reaction. In addition, [4Fe-4S]2+ cluster-bound ISCA1a/2, NFU4, and NFU5 were all found to be effective [4Fe-4S]2+ cluster donors for maturation of the mitochondrial apo-aconitase 2 as assessed by enzyme activity measurements. The results demonstrate rapid, unidirectional, and quantitative [4Fe-4S]2+ cluster transfer from ISCA1a/2 to NFU4 or NFU5 that further delineates their respective positions in the plant ISC machinery and their contributions to the maturation of client [4Fe-4S] cluster-containing proteins.

World in brief: Biden seeks to win over African leaders The World Today mhiggins.drupal 29 November 2022

At the US-Africa Leaders Summit, Washington will need to go beyond strategic geopolitical interest to compete with China’s offer, says James Orr.

African heads of state will join President Joe Biden in Washington in mid-December to take part in the second United States-Africa Leaders Summit. Some 50 African leaders will travel to the US capital for the two-day conference, which starts on December 13. 

Senior policymakers say  talks will focus on economic engagement, human rights, food security and climate, with an emphasis on partnerships that demonstrate an intention to go beyond strategic geo-political interest. President Biden has said he hopes to ‘reinforce the US-Africa commitment to democracy; mitigate the impact of Covid, respond to the climate crisis and amplify diaspora ties’. The White House will seek to offer reassurance to African governments concerned by a perceived cooling in relations with the US.

Africa’s cooling relations with the US 

‘As trite as it might sound, the key objective of this conference should really be to enhance trust between African leaders and the United States,’ said Gilbert Kaplan, a former under-secretary at the US Department of Commerce.  ‘I was in Africa in 2018 leading the President’s Advisory Council on Doing Business in Africa and the foreign minister of Ethiopia said to me: “Well you’re here but are you really here?” What he meant was: is the United States really committed to a long-term, strong relationship with the African continent or is it just a drive-through and a hello without making major commitments?’

The first summit was held by President Barack Obama in 2014. Addressing the inaugural conference, he spoke of the ‘blood of Africa’ that ran through his veins and how ‘the bonds between our countries are deeply personal’. Today, however, a succession of abstentions or no votes from African states over United Nations resolutions on Russia’s invasion of Ukraine points to a growing misalignment in the relationship. This is indicative of expanding Chinese and Russian influence on the continent, say analysts.

‘Africa is faced with some of the biggest governance challenges on the globe,’ said Kah Walla, president of the Cameroon People’s Party and the first woman in the nation’s history to run as a presidential candidate in 2011. ‘We need government that is functional, competent and innovative, and what we are getting instead is the US and a global international system that is supporting [via financial aid] autocratic and dysfunctional governments. We can’t continue in this way.’

Africa’s 54 nation states span six time zones and the continent’s population of 1.4 billion is on course to make up a quarter of the global population by 2050. It boasts the youngest demographic in the world, a potentially huge labour resource for private sector investors seeking to expand in manufacturing and processing, for example. The current median age in Africa is 18.8 years, compared with a global median age of 30.

In July this year, the US provided nearly $1.3 billion in humanitarian assistance to help stave off hunger due to drought in the Horn of Africa. Since June 2019, the US, under its Prosper Africa programme, has also helped close some 800 export and investment deals across 45 African nations with an estimated value of $50 billion.

The US is losing out to China in Africa

Meanwhile, China surpassed the US as Africa’s largest trade partner in 2009, with total bilateral trade reaching more than $254 billion in 2021, a 35 per cent rise on 2020.

‘Despite Africa’s tremendous economic potential, the US has lost substantial ground to traditional and emerging partners, especially China,’ Landry Signé, a member of the World Economic Forum’s Regional Action Group for Africa, told a Senate subcommittee on Africa last year. ‘While recent trends indicate that the US engagement with the region has fallen, it has not and should not cede its relationship with the region to other powers.’

National security and transatlantic unity top Biden’s agenda Expert comment NCapeling 1 February 2023

The timing of the State of the Union address is significant as Joe Biden tries to maintain focus on Ukraine and China while navigating partisanship at home.

Although primarily a domestic affair, the president’s remarks in the annual State of the Union (SOTU) telegraph the US’s foreign policy priorities to the rest of the world. They reveal the prism through which the US understands its national security and its role in the world.

The 2023 address comes as the first anniversary of the Ukraine invasion rapidly approaches, and the US is also trying to build international support for tough economic measures towards China. Domestically, Republican control of the House of Representatives will exert new pressure on US foreign policy choices. Electoral pressures are also around the corner and Joe Biden should announce soon whether he plans to run for president in 2024.

Alliances and partnerships are a driving force for Biden. As pressure to end the war in Ukraine intensifies, maintaining transatlantic unity on Ukraine will be a key priority for the president. And as US electoral politics rise to the fore, ensuring the visibility of Europe’s role as a security provider in its own backyard will be essential.

For a US president who sees China and the Indo-Pacific as America’s most significant long-term geopolitical challenge – and the only peer competitor to the US – transatlantic unity on China will also come under pressure.

Ukraine, China, and controlling domestic division

Republican leaders who now chair key committees in the House of Representatives continue to signal support for Ukraine, and polling shows a majority of US public opinion (54 per cent) favours sending either weapons or air defence systems.

But recent polls also reveal a growing gap between Democrat and Republican supporters with a slight majority of Republicans (52 per cent) now opposing further support for Ukraine.

Biden’s ability to demonstrate that America and its European partners remain unified in their policy towards Ukraine and Russia will help him ward off partisanship at home. Congressional approval of $45 billion for Ukraine in its end of year spending budget will help the president remain above the fray of partisan politics for now. But if there is an absence of clear signs Ukraine is succeeding in its war aims, the challenge of maintaining domestic support could become more difficult.

A greater US focus on China, India, and on the Indo-Pacific in 2023 is also likely. Bipartisanship is strong on China, Republicans are more singular and hawkish in their approach to China than the US president, and the public support tough measures. At a time of war in Europe, the president will place great emphasis on the need to maintain transatlantic unity and to work with a broader coalition of partners in the Indo-Pacific.

The groundwork for focus on China has already been laid. Throughout 2022, the Biden administration worked steadily, but quietly, to set out its China policy. Just before Russia’s invasion of Ukraine in February 2022, the US released its new US-Indo Pacific Strategy stressing regional partnerships – especially the Quad and ASEAN – and it’s determination to shape essential norms for the region.

It stated boldly – albeit in a document which was largely unnoticed – that the objective is not to change China but to ‘shape the strategic environment in which it operates’, a policy which signifies a clear departure from the more ideological approach pursued by the US during Donald Trump’s final year in office. And in May, the US announced its economic strategy for the region, the Indo-Pacific Economic Framework.

Now the terrain for competition with China and alignment with US partners and allies is heavily focused on technology and the daylight between national security and economic strategy in US China policy is rapidly disappearing. Biden’s policy focus of ‘invest (at home), align (with allies and partners), and compete (with China)’ has overshadowed earlier talk of cooperation between the US and China.

But as China opens its doors and Europe looks to bolster its economies, the US will have to work far harder to maintain transatlantic unity. Japan and the Netherlands embracing the US adoption of export controls on semiconductor chips is a positive sign, but the US’s economic strategy towards China may present harder choices to come for Europe. If China leans into its diplomacy, the pressure on transatlantic unity will also grow.

The new US Congress will only sharpen the president’s tough stance on China. Some Republican leaders still deny climate change and have demonstrated little interest in cooperating with China on debt relief for developing countries. The prospects for addressing critical global challenges appear dim without China’s collaboration, and the UK and Europe should work with the US president and Congress to correct this.

President Biden has clearly recognized that diplomacy will be critical in the months ahead. At a time of growing tensions, the potential for misperceptions or misunderstandings to create conflict will also increase, and so a US – and also European – commitment to broadening and deepening diplomacy with China is urgent but it also needs to be patient and sustained.

A new American industrial policy?

Those listening carefully to the State of the Union and who have followed US policy will also note the unchallenged hegemony of neoliberalism is rapidly vanishing. The American state appeared to be disturbingly absent in the early months of the US pandemic response – but now it is back and, in a surprising turn of events, is being cast as a solution not a problem.

Joris J. Benschop
Jul 1, 2007; 6:1198-1214
Research

Independent Thinking: Biden and the Republicans, AUKUS Audio NCapeling 9 February 2023

Episode 14 of our weekly podcast examines the Chinese balloon saga, the State of the Union address, and updates on the AUKUS deal 18 months after it was signed.

This week saw US president Joe Biden address an unruly joint session of Congress, during which he discussed America’s place in the world, China, Ukraine, and the state of the US economy.

As a new presidential cycle begins at the mid-point of Biden’s presidency, the panel analyses how Biden is doing and the challenges that lie ahead for him.

Also it is now 18 months since the announcement of the AUKUS partnership between the UK, US and Australia to develop nuclear hunter killer submarines. What does AUKUS mean for Australia, non-proliferation, and the Indo-Pacific more broadly?

Finally, the panel discusses China’s balloon over Montana in the US. Was it a signal from Beijing and what was it trying to photograph?

Joining guest host John Kampfner this week from Chatham House are Dr Leslie Vinjamuri, director of the US and the Americas programme and Dr Patricia Lewis, director of the International Security programme. They are joined by Dr Euan Graham, Shangri-La Dialogue senior fellow for defence and strategy at the International Institute for Strategic Studies (IISS).

About Independent Thinking

A weekly podcast hosted by Chatham House director Bronwen Maddox, in conversation with leading policymakers, journalists, and Chatham House experts providing insight on the latest international issues.

Members of the B-cell lymphoma (BCL-2) protein family regulate mitochondrial outer membrane permeabilization (MOMP), a phenomenon in which mitochondria become porous and release death-propagating complexes during the early stages of apoptosis. Pro-apoptotic BCL-2 proteins oligomerize at the mitochondrial outer membrane during MOMP, inducing pore formation. Of current interest are endogenous factors that can inhibit pro-apoptotic BCL-2 mitochondrial outer membrane translocation and oligomerization. A mitochondrial-derived peptide, Humanin (HN), was reported being expressed from an alternate ORF in the mitochondrial genome and inhibiting apoptosis through interactions with the pro-apoptotic BCL-2 proteins. Specifically, it is known to complex with BAX and BID. We recently reported the fibrillation of HN and BAX into β-sheets. Here, we detail the fibrillation between HN and BID. These fibers were characterized using several spectroscopic techniques, protease fragmentation with mass analysis, and EM. Enhanced fibrillation rates were detected with rising temperatures or pH values and the presence of a detergent. BID fibers are similar to those produced using BAX; however, the structures differ in final conformations of the BCL-2 proteins. BID fibers display both types of secondary structure in the fiber, whereas BAX was converted entirely to β-sheets. The data show that two distinct segments of BID are incorporated into the fiber structure, whereas other portions of BID remain solvent-exposed and retain helical structure. Similar analyses show that anti-apoptotic BCL-xL does not form fibers with humanin. These results support a general mechanism of sequestration of pro-apoptotic BCL-2 proteins into fibers by HN to inhibit MOMP.

Sylwia Struk
Dec 28, 2020; 0:RA119.001766v1-mcp.RA119.001766
Research

The F-box protein MORE AXILLARY GROWTH 2 (MAX2) is a central component in the signaling cascade of strigolactones (SLs) as well as of the smoke derived karrikins (KARs) and the so far unknown endogenous KAI2 ligand (KL). The two groups of molecules are involved in overlapping and unique developmental processes, and signal-specific outcomes are attributed to perception by the paralogous α/β-hydrolases DWARF14 (D14) for SL and KARRIKIN INSENSITIVE 2/ HYPOSENSITIVE TO LIGHT (KAI2/HTL) for KAR/KL. Additionally, depending on which receptor is activated, specific members of the SUPPRESSOR OF MAX2 1 (SMAX1) – LIKE (SMXL) family control KAR/KL and SL responses. As proteins that function in the same signal transduction pathway often occur in large protein complexes, we aimed at discovering new players of the MAX2, D14 and KAI2 protein network by tandem affinity purification using Arabidopsis cell cultures. When using MAX2 as a bait, various proteins were co-purified among which general components of the Skp1-Cullin-F-box complex and members of the CONSTITUTIVE PHOTOMORPHOGENIC 9 signalosome. Here, we report the identification of a novel interactor of MAX2, a type 5 serine/threonine protein phosphatase, designated PHYTOCHROME-ASSOCIATED PROTEIN PHOSPHATASE 5 (PAPP5). Quantitative affinity purification pointed at PAPP5 as being more present in KAI2 rather than D14 protein complexes. In agreement, mutant analysis suggests that PAPP5 modulates KAR/KL-dependent seed germination in suboptimal conditions and seedling development. Additionally, a phosphopeptide enrichment experiment revealed that PAPP5 might dephosphorylate MAX2 in vivo independently of the synthetic strigolactone analog, rac-GR24. Together, by analyzing the protein complexes to which MAX2, D14 and KAI2 belong, we revealed a new MAX2 interactor, PAPP5, that might act through dephosphorylation of MAX2 to control mainly KAR/KL- related phenotypes and, hence, provide another link with the light pathway.

Planet Fitness is seeking to further grow its number of locations in a second bid for the Blink Fitness chain of workout facilities, according to new reports.

The nation's depleted supply of IV solutions is recovering after a North Carolina facility recently resumed production after Hurricane Helene temporarily put it out of business.

President Joe Biden on Monday addressed a Veterans Day crowd at the national cemetery in Virginia where he revealed newly-expanded veteran benefits and urged Americans to "come together" and "keep faith."