Jennifer Lopez, Alex Rodriguez eyed a deal to work with biotech billionaire Wayne Rothbaum to mount the bid for the Mets

WASHINGTON: The woman who accused Joe Biden of sexually assaulting her in 1993 called on him to drop out of the US presidential race, saying Thursday she´d take a polygraph about the alleged encounter if he would. Tara Reade said in March that Biden, the Democratic Party´s presumptive...

President Donald Trump needled his Democratic rival Joe Biden Friday for limiting his campaign appearances to virtual events from the basement of his home in Delaware.

The United States is rallying support to back Taiwan's observer status in the World Health Assembly, the decision-making body of the World Health Organization, leading into its annual meeting May 17-21."Today, I want to call on all nations, including those in Europe, to support Taiwan's participatio...

Next-Generation Miniaturized Rubidium Atomic Clock Improves Performance and adds Features without Increasing Size

Microchip Expands Silicon Carbide (SiC) Family of Power Electronics to Provide System Level Improvements in Efficiency, Size and Reliability

This video defines State-Owned Enterprises (SOEs) and guides borrowers on how to assess their viability to participate as bidders under ADB funded projects.

This video explains Bidding Related Complaints, when they can occur in the procurement cycle, and why it is important for borrowers to address them in a timely manner.

NEW DELHI: Unitech Corporate Parks, a Unitech group firm listed in London, has received bids of up to Rs 2,700 crore from six companies, including Blackstone, for sale of its IT Special economic Zone (SEZ) in Gurgaon. Listed on London’s Alternative Investment Market and set up to invest in commercial real estate of India, UCP has 60 per cent stake in the Gurgaon SEZ comprising 3.6 million sq ft commercial space. Unitech has remaining stake in the SEZ. UCP has given the mandate to property consultant Jones Lang LaSalle India to find out potential buyers. According to sources, UCP, which has put the Gurgaon SEZ on the block, has received […]

One has been promoting environmental regulation for decades, while the other is bent on dismantling such policies. It makes for clear battle lines in the upcoming presidential election.

While President Donald Trump traveled to the battleground state of Arizona this week, his Democratic opponent for the White House, Joe Biden, campaigned from his basement as he has done throughout the coronavirus pandemic.

An in-depth study of Leonardo da Vinci's earliest-known drawing has proved definitively that the great Renaissance artist was in fact comfortable working with either hand. Dan Fastenberg reports.

Tara Reade, the woman who alleges Joe Biden sexually assaulted her in 1993, said in a video interview on Thursday that he should withdraw from the White House race. Gloria Tso reports.

In plants, water use efficiency (WUE) is a complex trait arising from numerous physiological and developmental characteristics. Here, we investigated the involvement of circadian regulation in long-term WUE in Arabidopsis (Arabidopsis thaliana) under light and dark conditions. Circadian rhythms are generated by the circadian oscillator, which provides a cellular measure of the time of day. In plants, the circadian oscillator contributes to the regulation of many aspects of physiology, including stomatal opening, rate of photosynthesis, carbohydrate metabolism, and developmental processes such as the initiation of flowering. We investigated the impact of the misregulation of numerous genes encoding various components of the circadian oscillator on whole plant, long-term WUE. From this analysis, we identified a role for the circadian oscillator in WUE. It appears that the circadian clock contributes to the control of transpiration and biomass accumulation. We also established that the circadian oscillator within guard cells can contribute to long-term WUE. Our experiments indicate that knowledge of circadian regulation will be important for developing crops with improved WUE.

The selection and firing of DNA replication origins play key roles in ensuring that eukaryotes accurately replicate their genomes. This process is not well documented in plants due in large measure to difficulties in working with plant systems. We developed a new functional assay to label and map very early replicating loci that must, by definition, include at least a subset of replication origins. Arabidopsis (Arabidopsis thaliana) cells were briefly labeled with 5-ethynyl-2'-deoxy-uridine, and nuclei were subjected to two-parameter flow sorting. We identified more than 5500 loci as initiation regions (IRs), the first regions to replicate in very early S phase. These were classified as strong or weak IRs based on the strength of their replication signals. Strong initiation regions were evenly spaced along chromosomal arms and depleted in centromeres, while weak initiation regions were enriched in centromeric regions. IRs are AT-rich sequences flanked by more GC-rich regions and located predominantly in intergenic regions. Nuclease sensitivity assays indicated that IRs are associated with accessible chromatin. Based on these observations, initiation of plant DNA replication shows some similarity to, but is also distinct from, initiation in other well-studied eukaryotic systems.

Photorespiration is an essential process in oxygenic photosynthetic organisms triggered by the oxygenase activity of Rubisco. In peroxisomes, photorespiratory HYDROXYPYRUVATE REDUCTASE1 (HPR1) catalyzes the conversion of hydroxypyruvate to glycerate together with the oxidation of a pyridine nucleotide cofactor. HPR1 regulation remains poorly understood; however, HPR1 phosphorylation at T335 has been reported. By comparing the kinetic properties of phosphomimetic (T335D), nonphosphorylatable (T335A), and wild-type recombinant Arabidopsis (Arabidopsis thaliana) HPR1, it was found that HPR1-T335D exhibits reduced NADH-dependent hydroxypyruvate reductase activity while showing improved NADPH-dependent activity. Complementation of the Arabidopsis hpr1-1 mutant by either wild-type HPR1 or HPR1-T335A fully complemented the photorespiratory growth phenotype of hpr1-1 in ambient air, whereas HPR1-T335D-containing hpr1-1 plants remained smaller and had lower photosynthetic CO₂ assimilation rates. Metabolite analyses indicated that these phenotypes were associated with subtle perturbations in the photorespiratory cycle of HPR1-T335D-complemented hpr1-1 rosettes compared to all other HPR1-containing lines. Therefore, T335 phosphorylation may play a role in the regulation of HPR1 activity in planta, although it was not required for growth under ambient air controlled conditions. Furthermore, improved NADP-dependent HPR1 activities in peroxisomes could not compensate for the reduced NADH-dependent HPR1 activity.

Phosphatidylcholine and phosphatidylethanolamine are two major phospholipid classes in eukaryotes. Each biosynthesis pathway starts with the phosphorylation of choline (Cho) or ethanolamine (Etn) catalyzed by either choline or ethanolamine kinase (CEK). Arabidopsis contains four CEK isoforms, but their isozyme-specific roles in metabolism and development are poorly described. Here, we showed that these four CEKs have distinct substrate specificities in vitro. While CEK1 and CEK2 showed substrate preference for Cho over Etn, CEK3 and CEK4 had clear substrate specificity for Cho and Etn, respectively. In vivo, CEK1, CEK2, and CEK3 exhibited kinase activity for Cho but not Etn, although the latter two isoforms showed rather minor contributions to total Cho kinase activity in both shoots and roots. The knockout mutants of CEK2 and CEK3 both affected root growth, and these isoforms had nonoverlapping cell-type-specific expression patterns in the root meristematic zone. In-depth phenotype analysis, as well as chemical and genetic complementation, revealed that CEK3, a Cho-specific kinase, is involved in cell elongation during root development. Phylogenetic analysis of CEK orthologs in Brassicaceae species showed evolutionary divergence between Etn kinases and Cho kinases. Collectively, our results demonstrate the distinct roles of the four CEK isoforms in Cho/Etn metabolism and plant development.

In plant–pathogen relations, disease symptoms arise from the interaction of the host and pathogen genomes. Host–pathogen functional gene interactions are well described, whereas little is known about how the pathogen genetic variation modulates both organisms’ transcriptomes. To model and generate hypotheses on a generalist pathogen control of gene expression regulation, we used the Arabidopsis thaliana–Botrytis cinerea pathosystem and the genetic diversity of a collection of 96 B. cinerea isolates. We performed expression-based genome-wide association (eGWA) for each of 23,947 measurable transcripts in Arabidopsis (host), and 9267 measurable transcripts in B. cinerea (pathogen). Unlike other eGWA studies, we detected a relative absence of locally acting expression quantitative trait loci (cis-eQTL), partly caused by structural variants and allelic heterogeneity hindering their identification. This study identified several distantly acting trans-eQTL linked to eQTL hotspots dispersed across Botrytis genome that altered only Botrytis transcripts, only Arabidopsis transcripts, or transcripts from both species. Gene membership in the trans-eQTL hotspots suggests links between gene expression regulation and both known and novel virulence mechanisms in this pathosystem. Genes annotated to these hotspots provide potential targets for blocking manipulation of the host response by this ubiquitous generalist necrotrophic pathogen.

Vitamin A has diverse biological functions and is essential for human survival at every point from embryogenesis to adulthood. Vitamin A and its derivatives have been used to treat human diseases including vision diseases, skin diseases, and cancer. Both insufficient and excessive vitamin A uptake are detrimental, but how its...

The most fundamental genetic program of an annual plant defines when to grow and reproduce and when to remain dormant in the soil as a seed. With the right timing, plants can even live in hostile regions with only a few months of growth-favorable abundant rains and mild temperatures. To...

In Arabidopsis, LTR retrotransposons are activated by mutations in the chromatin gene DECREASE in DNA METHYLATION 1 (DDM1), giving rise to 21- to 22-nt epigenetically activated siRNA (easiRNA) that depend on RNA DEPENDENT RNA POLYMERASE 6 (RDR6). We purified virus-like particles (VLPs) from ddm1 and ddm1rdr6 mutants in which genomic RNA is reverse transcribed into complementary DNA. High-throughput short-read and long-read sequencing of VLP DNA (VLP DNA-seq) revealed a comprehensive catalog of active LTR retrotransposons without the need for mapping transposition, as well as independent of genomic copy number. Linear replication intermediates of the functionally intact COPIA element EVADE revealed multiple central polypurine tracts (cPPTs), a feature shared with HIV in which cPPTs promote nuclear localization. For one member of the ATCOPIA52 subfamily (SISYPHUS), cPPT intermediates were not observed, but abundant circular DNA indicated transposon "suicide" by auto-integration within the VLP. easiRNA targeted EVADE genomic RNA, polysome association of GYPSY (ATHILA) subgenomic RNA, and transcription via histone H3 lysine-9 dimethylation. VLP DNA-seq provides a comprehensive landscape of LTR retrotransposons and their control at transcriptional, post-transcriptional, and reverse transcriptional levels.

Background:

Patients with multimorbidity often require services across different health care settings, yet team processes among settings are rarely implemented. We explored perceptions of specialists and family physicians collaborating in a telemedicine interprofessional consultation for patients with multimorbidity to better understand the value of bringing physicians together across the boundaries of health care settings.

Last week, former Vice President Joe Biden told the world that he “unequivocally” denied accusations by Tara Reade, a former staffer in his Senate office, that he sexually assaulted her in the early ’90s.

On Friday evening, Reade responded: Prove it.

“Joe Biden should take the polygraph,” Reade told former television anchor Megyn Kelly, in an interview that aired on Kelly’s YouTube channel. “I will take one if Joe Biden takes one, but I’m not a criminal.”

Read more at The Daily Beast.

Michigan can and will recover from the devastating COVID-19 pandemic - but not if Joe Biden wins the presidential election this November. The former vice president's radical policy platform is destined to sabotage America's second economic comeback, dooming the Great Lakes State to a bleak future of perpetual economic stagnation.

If he can win the battle for our screens, he can benefit from the death of the traditional presidential campaign.

In light of the newly declassified documents, it is clear that understanding what happened in that January 5 Oval Office meeting is essential to understanding the corrupt operation against the Trump administration.

"I just couldn't be more proud of you and the prospect of your presidency," Newsom told Biden Friday during a campaign event.

Bill Maher said Republicans "don't care" about Tara Reade's sexual assault allegations, saying such claims are simply used as a "unilateral weapon" against Democrats.

A federal judge on Friday declined to dismiss the charges against "Full House" actress Lori Loughlin and other wealthy parents awaiting trial in the U.S. college admissions scandal after they accused investigators of fabricating evidence.

The notoriously independent-minded federal judge who once said he was disgusted by the conduct of Michael Flynn could block the administration's bid to drop criminal charges against the former adviser to President Donald Trump, legal experts said.

While President Donald Trump traveled to the battleground state of Arizona this week, his Democratic opponent for the White House, Joe Biden, campaigned from his basement as he has done throughout the coronavirus pandemic.

President Donald Trump’s re-election campaign launched a massive, multi-million dollar ad blitz against presumptive Democratic presidential nominee Joe Biden on Thursday, its biggest onslaught against Biden since the coronavirus pandemic hit the United States.

The woman who alleges presumptive Democratic presidential nominee Joe Biden sexually assaulted her in 1993 said in a video interview on Thursday that he should withdraw from the White House race.

Allies of presumptive Democratic presidential nominee Joe Biden are being told to sharpen attacks on President Donald Trump's stimulus efforts as thinly veiled "cronyism," according to a memo being sent to Democratic officeholders and supporters on Friday.

While President Donald Trump traveled to the battleground state of Arizona this week, his Democratic opponent for the White House, Joe Biden, campaigned from his basement as he has done throughout the coronavirus pandemic.

The Trump campaign released a new ad in Spanish targeting Democrats for their response to the allegations Tara Reade made against presumptive Democratic nominee Joe Biden.

Tara Reade has claimed in a new interview that Joe Biden used graphic language to proposition her for sex when he allegedly assaulted her in 1993.

Tara Reade addressed her past praise for Joe Biden during an interview released on Friday as well as her cryptic "tic toc" tweet skeptics have pointed to while assessing her claims. 

Joe Biden's campaign has signed a joint fundraising agreement with the DNC that will help Democrats close the gap with President Donald Trump's massive war chest.

Bernie Sanders' bid to collect convention delegates hits snag as New York cancels Democratic presidential primary

Hillary Clinton, the first woman to win a major party’s presidential nomination, teased an expected endorsement of Joe Biden for president Tuesday in a tweet.

Rep. Justin Amash announced he's seeking the Libertarian Party's presidential nomination — a move which history suggests will hurt the Democratic candidate, but could pull votes from both Biden and Trump.

President Trump Thursday said he thinks former Vice President Joe Biden should respond to a claim from a former staffer that he assaulted her in 1993 even as he cast doubt on the veracity of the allegation.

The apparent Democratic nominee called for an immediate $13 minimum wage increase for essential workers and criticized President Trump for viewing these front-liners as “disposable."

A new poll from Montana State University shows Democratic Gov. Steve Bullock leading incumbent GOP Sen. Steve Daines, and Joe Biden inching toward President Trump's lead.

Joe Biden's campaign is gaining the support of key progressive groups to unite the party's factions.

In her first on-camera interview since the former vice president “unequivocally” denied the sexual assault allegation, Reade says she's willing to be questioned under oath.

Joseph R. Biden said Friday President Trump's ham-handed response to the coronavirus has helped to steer the nation into a Great Depression-like economic tailspin.

Mr. Biden said Mr. Trump "utterly failed" to prepare for COVID-19 and said the virus has "laid bare" the damage Mr. Trump has done by pursuing ...