TNF is a highly pro-inflammatory cytokine that contributes not only to the regulation of immune responses but also to the development of severe inflammatory diseases. TNF is synthesized as a transmembrane protein, which is further matured via proteolytic cleavage by metalloproteases such as ADAM17, a process known as shedding. At present, TNF is mainly detected by measuring the precursor or the mature cytokine of bulk cell populations by techniques such as ELISA or immunoblotting. However, these methods do not provide information on the exact timing and extent of TNF cleavage at single-cell resolution and they do not allow the live visualization of shedding events. Here, we generated C-tag TNF as a genetically encoded reporter to study TNF shedding at the single-cell level. The functionality of the C-tag TNF reporter is based on the exposure of a cryptic epitope on the C terminus of the transmembrane portion of pro-TNF on cleavage. In both denatured and nondenatured samples, this epitope can be detected by a nanobody in a highly sensitive and specific manner only upon TNF shedding. As such, C-tag TNF can successfully be used for the detection of TNF cleavage in flow cytometry and live-cell imaging applications. We furthermore demonstrate its applicability in a forward genetic screen geared toward the identification of genetic regulators of TNF maturation. In summary, the C-tag TNF reporter can be employed to gain novel insights into the complex regulation of ADAM-dependent TNF shedding.

12th International Forum on Illegal, Unreported and Unregulated Fishing 18 May 2020 TO 22 May 2020 — 2:00PM TO 3:30PM Anonymous (not verified) 27 September 2019

When viewing a SAS Visual Analytics report, you might intermittently see an error that includes content similar to the following:

SAS Web Report Studio enables you to link reports based on a group break value. However, when you click the link, it might fail with an HTTP 400 error. The exact message you see depends on which browser you are u

A comprehensive and standardized system to report lipid structures analyzed by MS is essential for the communication and storage of lipidomics data. Herein, an update on both the LIPID MAPS classification system and shorthand notation of lipid structures is presented for lipid categories Fatty Acyls (FA), Glycerolipids (GL), Glycerophospholipids (GP), Sphingolipids (SP), and Sterols (ST). With its major changes, i.e., annotation of ring double bond equivalents and number of oxygens, the updated shorthand notation facilitates reporting of newly delineated oxygenated lipid species as well. For standardized reporting in lipidomics, the hierarchical architecture of shorthand notation reflects the diverse structural resolution powers provided by mass spectrometric assays. Moreover, shorthand notation is expanded beyond mammalian phyla to lipids from plant and yeast phyla. Finally, annotation of atoms is included for the use of stable isotope-labeled compounds in metabolic labeling experiments or as internal standards. This update on lipid classification, nomenclature, and shorthand annotation for lipid mass spectra is considered a standard for lipid data presentation.

Coronavirus disease 2019 (COVID-19) is a highly contagious infection and threating the human lives in the world. The elevation of cytokines in blood is crucial to induce cytokine storm and immunosuppression in the transition of severity in COVID-19 patients. However, the comprehensive changes of serum proteins in COVID-19 patients throughout the SARS-CoV-2 infection is unknown. In this work, we developed a high-density antibody microarray and performed an in-depth proteomics analysis of serum samples collected from early COVID-19 (n = 15) and influenza (n = 13) patients. We identified a large set of differentially expressed proteins (n = 132) that participate in a landscape of inflammation and immune signaling related to the SARS-CoV-2 infection. Furthermore, the significant correlations of neutrophil and lymphocyte with the CCL2 and CXCL10 mediated cytokine signaling pathways was identified. These information are valuable for the understanding of COVID-19 pathogenesis, identification of biomarkers and development of the optimal anti-inflammation therapy.

Impact report 2020–21 Other resource dora.popova 14 July 2021

Explore our policy impact in the past year, including through our research, convening and next generation initiatives, in a revised format annual review.

Chair’s statement

It has been an extraordinary year of change for us all. The COVID-19 pandemic is the greatest challenge to the world for generations. Millions have lost their lives or suffered devastating impacts on their health, both from COVID-19 itself and because health systems have been unable to deliver treatments for other conditions. The pandemic has also caused the greatest shock to the global economy since the 1930s, setting back the progress of recent years in eliminating poverty, getting more children into education and improving global health.

This means Chatham House is needed more than ever. Our world-leading convening and cutting-edge research on the major challenges facing the world, from building more sustainable economic growth and tackling climate change to easing geopolitical tensions, has continued despite the challenges of lockdown. For that I pay tribute to the resilience and ingenuity of the staff who have found innovative solutions to the obstacles presented by the pandemic, all while working largely from home.

This year I am also delighted to note the exceptional gift of £10 million from the MAVA Foundation to enable us to launch the Sustainability Accelerator. This initiative builds on the Hoffmann Centre’s last five years of innovative convening and activity. And it puts sustainability at the core of the institute’s work in this critical year for addressing climate change and biodiversity.

So, as I prepare to step down from my three years as chair of the institute, I would like to thank my fellow Council members and Robin Niblett and his team for their hard work and ambition to deliver on Chatham House’s mission. I have also been especially pleased to see how we are engaging younger, more diverse audiences through the next generation initiatives, including our Panel of Young Advisers, the Common Futures Conversations project, the QEII Academy Ambassadors, our Internship Programme and the Chatham House-SNF CoLab.

I am particularly pleased about the Chatham House Summer School, where 16–18-year-olds can now engage with experts on international affairs and get an insight into careers within the charity and not-for-profit sector.

It has been an honour to lead this extraordinary institution and I look forward to continuing my involvement with Chatham House in new ways.

Lord Jim O’Neill

SpaceX has reportedly struck a deal with LG Energy Solution Ltd. to supply cylindrical lithium-ion batteries for Elon Musk's Starship rocket, expected to launch early next year, according to industry sources.

U.S. Defense Secretary Lloyd Austin has pledged to get to the bottom of reports of military personnel experiencing mysterious illnesses while overseas.

HSINCHU, Taiwan, Nov. 8, 2024 – TSMC (TWSE: 2330, NYSE: TSM) today announced its net revenue for October 2024. On a consolidated basis, revenue for October 2024 was approximately NT$314.24 […]

The post TSMC Releases October 2024 Revenue Report appeared first on HPCwire.

Intersect360 Research has released a comprehensive new report concerning the challenges facing the combined fields of high-performance computing (HPC) and artificial intelligence (AI). Titled “Issues Facing the HPC-AI Industry: Insights […]

The post Report from HALO Details Issues Facing HPC-AI Industry appeared first on HPCwire.

AI may be a priority at American companies, but the difficulty in managing data and obtaining high quality data to train AI models is becoming a bigger hurdle to achieving […]

The post AI Has a Data Problem, Appen Report Says appeared first on HPCwire.

SpaceX has reportedly struck a deal with LG Energy Solution Ltd. to supply cylindrical lithium-ion batteries for Elon Musk's Starship rocket, expected to launch early next year, according to industry sources.

The state, which earned a B, saw its educational strength buoyed by strong performance in areas that measure socioeconomic factors that can affect the educational environment.

The state, which earned a B-plus, led the nation in K-12 achievement rankings and outperformed other states in several key academic indicators, but fell short on funding equity.

The state, which earned a D-plus, was weak on socioeconomic factors that can affect the educational environment, and also in the school finance area.

The state, which earned a D-plus, has struggled with school finance issues and endured teacher strikes and battles over pay, but also earned B-plus for funding equity.

Private schools in New York soon will be required to report suspected sexual abuse of students in their schools to law enforcement, bringing the independent schools under the same rules as public schools.

Most New Jersey students get schooled in the arts, but time devoted to the subject has been dwindling.

School authorities in Utah have failed to report educator misconduct, possibly allowing teachers to offend again by moving to other schools, according to a new audit.

The state, which earned a B, is one of the nation’s wealthiest and turned in strong performances in the school finance arena and in areas such as preschool and kindergarten enrollment.

Victoria Petersen, of the Peninsula Clarion on the Kenai Peninsula, will report on the challenges of rural education, especially in a state as vast as Alaska.

The state, which earned a D-plus, scored low on the Chance for Success Index, which tracks a host of socioeconomic factors that can affect the educational environment.

Academic and health offerings have increased in McDowell County, W.Va., due to a private-public partnership.

Today, FAO launched two new reports in time for the 160^th session of the Council:

-          FAO + Belgium: Inclusive approaches to end hunger and promote [...]

Help shape FAO’s digital publishing programme by answering a 5-minute survey on the The State of the World’s Forests (SOFO) and Global Forest Resources Assessment (FRA).

The survey seeks to gather [...]

In 2020, FAO’s flagship ‘The State of the World’ collection was released in a new easy-to-read digital format, providing audiences with a responsive reading experience to discover FAO’s work and [...]

In December 2017, the United Nations General Assembly proclaimed the UN Decade of Family Farming 2019-2028 (UNDFF) to serve as a framework for developing public policies and investments to [...]

In an exceptionally challenging biennium, see how FAO rose to the challenge, implementing a global, holistic and multipronged strategy to support Members.

This newly-released report highlights the progress made since FAO’s Strategy for Private Sector Engagement 2021-2025 was approved during the 165th session of the Council in December 2020. It discusses important [...]

This newly-released annual report charts progress in FAO's work with the private sector in 2022. It highlights developments across FAO's portfolio of private sector engagements, documents major achievements and lessons learned [...]

This annual report charts progress in FAO's work with the private sector in 2022. It highlights developments across FAO's portfolio of private sector engagements, documents major achievements and lessons [...]

In December 2017, the United Nations General Assembly proclaimed the UN Decade of Family Farming 2019-2028 (UNDFF) to serve as a framework for developing public policies and investments to [...]

Fish biomass faces steep falls by end of century under high-emissions scenario

Smithsonian staff writer Abigail Tucker came across imperiled zebras, dusty savannahs and perilous roads while researching the Tanzanian lions. Read more at http://www.smithsonianmag.com/science-nature/The-Truth-About-Lions.html

Though breast cancer mortality is declining overall, Asian American women and women under 50 have experienced an uptick in diagnoses of the disease

An assessment from the International Union for Conservation of Nature paints a grim picture of the extinction risk of the world's trees

As people across the country mourn the Honourable Justice Murray Sinclair, some in the Northwest Territories justice system are reflecting on his contributions to the country — one of those being Gladue principles. 

Kimberly Murray has opened an uncomfortable and difficult but long overdue conversation about justice for Canada’s "disappeared" residential school children, Indigenous leaders say in response to the special interlocutor's two-volume final report.

Record revenue of $1.6 billion Record quarterly ADV of 28.3 million contracts - the second consecutive quarter of volume, open interest growth across every asset class CHICAGO, Oct. 23, 2024...

Record October ADV for interest rate, energy, metals and agricultural products Record October ADV across U.S. Treasury and SOFR complexes All-time record monthly ADV in U.S. Treasury options and...

OM Montenegro put on a two week Beach Outreach in the city of Bar.

Explore volume, open interest and other trading highlights across the suite of Agricultural futures and options with the Q4 2024 Agricultural Update.

Infrastructure, materials, and mining group Raubex has reported a sharp climb in interim profit, helped by a pickup in SA road construction activity.

After a day of ESSA discussion as part of Education Week's "Keys to ESSA Readiness" online event, Lisa Stark, Catherine Gewertz, and Alyson Klein provide key takeaways.

The Center for American Progress has released a set of recommendations for governors in 2019 that includes things such as full-day universal preschool and the study of pre-term births, which can cause learning difficulties.

Veteran reporter Debra Viadero invites researchers to scrutinize her decades of reporting for racial bias.