The present invention relates to the field of organic chemistry and in particular to organic free radicals used as polarizing agents in the technique of Dynamic Nuclear Polarization (DNP), which involves transferring the polarization of electron spins to the nuclei of a compound whose Nuclear Magnetic Resonance (NMR) is being observed. It concerns Dinitroxide-type Biradical polarizing agents characterized by a rigid linkage between the aminoxyl groups of said nitroxide units. This particular structure enables, at low temperatures and high fields, optimal transfer of polarization and optimal enhancement of NMR/MAS signals of the polarized nuclei of the compound studied.

Plant viral vectors have great potential in rapid production of proteins, but no simple. Here a geminivirus-based system for high-yield and rapid production of oligomeric protein complexes, including virus-like particle (VLP) vaccines and monoclonal antibodies (mAbs) is described. In particular, a single vector that contains two non-competing replicons for transient expression in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures (VLPs or full-size mAb) is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of this technology for producing multiple-subunit protein complexes.

The invention relates to compositions comprising vasoactive intestinal peptide (VIP) or fragments thereof, and the use of such compositions in the treatment of kidney disease, in particular kidney fibrosis, and other associated conditions.

Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.

A method for detecting electromagnetic waves derived from bacterial DNA, comprising extracting and purifying nucleic acids from a sample; diluting the extracted purified nucleic acids in an aqueous solvent; measuring a low frequency electromagnetic emission over time from the diluted extracted purified nucleic acids in an aqueous solvent; performing a signal analysis of the low frequency electromagnetic emission over time; and producing an output, based on the signal analysis, in dependence on the DNA in the sample. The DNA may be extracted from at least one of blood, feces, urine, saliva, tears, seminal fluid, sweat, seminal and vaginal fluids of a patient, or water to determine, e.g., potability. The samples may be frozen. The extracting and purifying may comprise diluting the sample with an aqueous buffer and mixing; degrading proteins in the diluted sample; precipitating DNA from the buffer solution; and resuspending the precipitated DNA in an aqueous solution.

This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

A method of making a vehicle seat assembly comprising providing a cushion having an “A” surface, a “B” surface, a central portion, and two bolster areas, with each bolster area being adjacent the central portion, the cushion having a plurality of intrusions extending from the “A” surface towards the “B” surface to form a hardness gradient between at least one of the bolster areas and the central portion of between 8% to 25%, securing the cushion to a frame, and covering cushion with a trim member.

A cushion element for a vehicle seat or headrest comprises a non-woven layer and a fiber composite material. An insert material is arranged between the non-woven layer and the fiber composite material, and has different compression properties than the fiber composite material. The fiber composite material forms together with the insert material and the non-woven layer a one-piece material composite.

A bonded, direct fixation fastener as supportive structure for a rail on a railroad tie or bed comprising: a rail plate, wherein the rail plate has a generally horizontal center portion having a lower face and an upper face and defining a rail plate horizontal plane, and wherein the rail plate center portion extends at a first angle from opposing sides as rail plate tongues above the rail plate horizontal plane, the rail plate having a rail securing device; a bed plate comprising a generally horizontal center portion, an upper bed plate face defining a bed plate horizontal plane, and a pair of opposing bed plate tongues extending from opposing sides of the bed plate horizontal center portion disposed at a second angle above the bed plate horizontal plane; a horizontal elastomeric portion of relatively lower durometer hardness encapsulating at least a portion of the bed plate and at least a portion of the rail plate; and vertical elastomeric portions of relatively higher durometer hardness encapsulating at least a portion of each bed plate tongue and at least a portion of each rail plate tongue, and surrounding the upper face of the rail plate; and wherein each vertical elastomeric portion is physically continuous with the horizontal elastomeric portion.

A railroad coupler knuckle includes a single, solid rib at a horizontal centerline of the knuckle that passes through a pivot pin hub thereof. The single, solid rib extends generally from a flag hole of a finger cavity of the knuckle to an opposite side of the knuckle from the flag hole. In another aspect, a railcar coupler knuckle includes a tail section, a hub section, and a nose section. The tail, hub, and nose sections define internal cavities including (i) a combined void that defines a pivot pin hub cavity and a kidney cavity and (ii) an isolated finger cavity. The combined void is formed using a first internal core during manufacturing of the coupler knuckle. The isolated finger cavity is formed using a second internal core during manufacturing of the coupler knuckle, such that molten alloy substantially separates the combined void and the isolated finger cavity.

A wire coloring apparatus including a roller is provided to prevent drag of a colorant, which is jetted on an electric wire and not dried yet, the guide roller is arranged at a downstream side of a coloring nozzle, which jets a colorant toward a highest point of an outer surface of the electric wire moving along a lengthwise direction thereof, in a moving direction of the electric wire. The guide roller is formed with a pair of tapered surfaces catching and supporting the electric wire and approaching to each other in accordance with nearing to a rotating axis. When a radius REW of the electric wire 3 is 1.2 mm, an angle θT formed by the pair of tapered surfaces is designed at least 60 degrees and not more than θX degrees.

The present invention includes a telegraph controller and an FSK modulator that serve as a signal transmission unit that creates a checking telegraph that is different from a controlling telegraph and transmits the checking telegraph to a pickup coil, an FSK demodulator and a telegraph controller that serve as a signal reception unit that restores telegraph information from the checking telegraph received via the pickup coil, and a communication controller that compares transmission telegraph information included in a checking telegraph transmitted from the signal transmission unit with reception telegraph information restored from a received checking telegraph that includes the transmission telegraph information to verify a telegraph reception function on an on-board side.

A batch reactor process for the synthesis of potassium 5,7-dinitro-[2,1,3]-benzoxadiazol-4-olate-3-oxide (KDNP) from 3-bromo-2,4,6-trinitroanisole (ETNA) includes adding BTNA to a reaction vessel containing potassium azide (KN3) and water (H2O). The resulting mixture is heated to 90° C. followed by cooling to room temperature and agitating the final solution. The precipitate KDNP product is recovered by filtration.

A system for determining an optimal hardness of a patient interface device includes a fit score determination unit structured to receive a 3-D model of the patient interface device and a 3-D model of a patient's face and to determine a fit score between the patient interface device and the patient's face based on the 3-D model of the patient interface device and the 3-D model of the patient's face, and a hardness determination unit structured to determine a hardness value of the patient interface device based on the determined fit score.

Methods of screening for expression of an RNA associated with a post-kidney transplant complication include collecting vesicles from urine, isolating vesicle-associated RNA, and analyzing expression patterns. In particular, AIF1, BTN3A3, CCL5, CD48, HAVCR1, or SLC6A6 mRNA expression patterns are analyzed.

THERE’S no doubt that the Prime Minister spoke from the heart when he addressed the Tory conference in Birmingham this week, speaking of the NHS.

News you don't want to miss for April 8:

1. Schools in Alameda County, and five other Bay Area counties, will not re-open this academic year, the San Francisco Chronicle reports.…

News you don't want to miss for April 15:

1. The number of covid-19 deaths in Alameda County increased from 23 to 36 over a two-day period, SFGate reports.…

News you don't want to miss for April 22:

1. Oakland is looking at a potentially historic $80 million budget shortfall over the next year, Bay City News reports.…

News you don't want to miss for April 29:

1. Alameda County and five other Bay Area counties issued a revised shelter in place order on Wednesday that will allow for some outdoor activities to resume, such as, construction work, landscaping, gardening, golf and tennis, SFGate reports.…

News you don't want to miss for May 6:

1. Former Oakland Chief of Police Anne Kirkpatrick filed a lawsuit alleging the Oakland Police Commission, which fired her earlier this year, did so as retaliation for Kirkpatrick blowing the whistle on their own wrongdoing, including seeking personal favors, the San Francisco Chronicle reports.…

FOOTBALLING miracles do sometimes happen, but the chances of Saints getting one to keep them up looks very slim indeed.

Hillsborough, League One, Saturday September 25, 2010

In this month's edition of AppleVis Unleashed, Thomas Domville, Randy Rusnak, and Mike Malarsie discuss recent Apple news and other topics of interest. Topics featured in this podcast include:

• iPad at 10: Why apps made the iPad a success
• Apple Q1 2020: The company cracks $90B in a single quarter for the first time as the iPhone returns to glory
• Things Apple Didn't Want to Talk About in its Q1 Earnings Report
• Apple rings in new era of Services following landmark year
• Apple's App Store generates over a billion dollars per week
• Apple Releases iOS 13.3.1 and iPadOS 13.3.1 with Some Improvements to Smart Invert
• Apple Planning to Make Original Podcasts Promoting Its TV Shows
• Apple Requiring Notarization for Non Mac App Store Apps Starting February 2020
• Apple's Upcoming Low-Cost iPhone Could Launch as Early as March
• Kuo: Apple to Launch AirTags, Small Charging Mat, New iPads and Macs, High-End Headphones, and More in First Half of 2020
• Rumor: iOS 14 to support all of the devices as iOS 13
• How to Enable Low Data Mode on iPhone Cellular to Reduce Mobile Data Use
• How to clear all your notifications at once on Apple Watch

You can contact the Unleashed Team with feedback or questions at unleashed@applevis.com

Adopted from Texas and raised in Mississippi, Krista Shows was a kid who grew up singing in church. She spent time in Los Angeles, Hawaii, and Western North Carolina before returning to Mississippi in her early 20s. She's now back in Asheville, and has a sweet blend of folk, R&B, and country influences. She performs (with a full band!) at Isis Restaurant & Music Hall in Asheville on Saturday the 22nd.

Tom Rush helped shape the folk revival in the ’60s and the renaissance of the ’80s and ’90s, his music having left its stamp on generations of artists. James Taylor told Rolling Stone, “Tom was not only one of my early heroes, but also one of my main influences.” His voice and guitar skills remain as rich and vibrant as ever, and we are honored to host him for a live morning session before his concert at Isis Restaurant & Music Hall Wednesday evening.

This Charlotte band started off as a solo project of singer/guitarist and SC native Jeremy Davis, but their sound has morphed to the great fiddle/mandolin/bass lineup they have today. And their indie-rock roots still show with a new cover of My Morning Jacket's "Golden". We welcome them back to Studio B in advance of their Friday the 13th show at Pisgah Brewing in Black Mountain.

During the WCBE spring fund drive the staff was so inspired by your responses that we promised to each do five random acts of kindness in your honor. Coming up with ideas wasn’t hard, and putting them into action was even easier. In fact, I realized there were different categories of RAK. Some of the things I did in your name were not-so-random; every week when I do my grocery shopping now, I spend $5-10 on food for the free Little Pantry in my neighborhood, to provide a snack or an extra meal for someone who needs it. Some were practical, like taking cat litter and a dozen cans of prescription food to the folks at Cat Welfare. Some were more… whimsical. I used scraps of old sweaters to make a dozen or so little monsters – then scattered them around the children’s sections of two different libraries. Some of the best RAKs, the ones that make people smile, are small, spontaneous gestures. In the course of this challenge to myself, I realized how many opportunities there are in a day for

Victory in Europe sparked scenes of delirium across Britain as people flooded on to the streets to toast the downfall of Hitler’s regime.

A judge has cleared the way for a DNA test in a last-ditch effort to keep a dog from being euthanized in the death of a neighbor's pet.

Police say a member of the school board in Connecticut's largest city posed as a police officer and attempted to kidnap a man at gunpoint.

Plugin Boutique has announced a sale on Signum Audio, offering discounts of up to 60% off for a limited time. DSP engineering experts Signum Audio develop a range of high-end and highly intuitive plugins for advanced loudness analysis, limiting and normalisation. Save up to 60% off their entire range, exclusively at Plugin Boutique. The sale […]

The post Get 60% off Signum Audio’s loudness analysis, limiting & normalization plugins appeared first on rekkerd.org.

Over the past few weeks, chief executives of Unilever, Mondelez, Hershey's Procter & Gamble, Coca-Cola and Kimberly Clark have said India’s Covid-19 lockdown protocols had led to severe supply chain disruptions and labour shortages, hurting business in the key market.

Outpatient dialysis facilities have also reported challenges, including separating COVID-19 positive and negative patients and protecting their own workers.

“50% of those surveyed said they were not able to buy at all due to no availability while 26% said they had to buy an unknown brand as reputed brands were out of stock. 8% said they were able to buy reputed sanitizers at a general retail store and 15% said they were able to buy reputed brand sanitizers at a chemist,” LocalCircle survey said.

On today's show, we have highlights from the governor's daily coronavirus response briefing. Plus, we find out how local animal shelters are faring during the COVID-19 pandemic. And, we hear about a new grant program designed to help arts and cultural nonprofits weather the outbreak.

On today's show, we have the latest update from Governor Asa Hutchinson's daily coronavirus response briefing. Plus, we hear from advocates at the Children's Safety Center of Washington County about how they're continuing to organize trainings during the pandemic. And, we mark the 50th anniversary of Earth Day with the founder of the OMNI Center for Peace, Justice and Ecology.

On today's show, we have the latest updates from Governor Asa Hutchinson's daily coronavirus response briefing. Plus, we hear from local law enforcement officers about the changes they've had to make in their daily operations and why that has resulted in fewer bookings at county jails. And, we speak with Eddie Schmeckenbecher, who stands by the governor's side every day during his COVID-19 briefings, about what it's been like to be Arkansas's American Sign Language translator during the pandemic.

On today's show, we hear what the governor had to say during his latest coronavirus response briefing. Plus, we find out how Arkansas business owners and bankers are navigating the Paycheck Protection Program application process. And, we speak with local farmers about the types of adjustments they've had to make to get their products to customers.

Michael Cleveland has been called “one of the premier fiddle players of his generation, if not in all of bluegrass history.” He's also been completely blind since birth.

Pianist Roman Rabinovich's deep dive into the Haydn sonatas brought him revelations. Because Haydn's life bridged both the Baroque and Classical periods, his compositions reflect how the technology of the piano constantly progressed. "Instruments really changed like computers today, at that pace," Rabinovich said. Uzbeki-born, Israeli-trained Rabinovich continues to reflect Haydn's compositional invention since the Haydn project's debut at the Lincoln Center by performing the sonatas internationally. To listen to the full interview with Of Note's Katy Henriksen, click on the streaming link above.

For the first time in a decade Congress will hold a hearing Wednesday on the subject of reparations for the descendants of slaves in the United States, a topic that has gained traction in the run-up to the 2020 elections. The hearing is set for June 19, also known as "Juneteenth," the day when in 1865 former enslaved people in Texas first learned that they had been emancipated two years earlier. The House Judiciary Subcommittee on the Constitution, Civil Rights and Civil Liberties is holding the hearing on H.R. 40 , which calls for a commission to "study and develop reparation proposals for African-Americans," including a formal apology by the U.S. government "for the perpetration of gross human rights violations and crimes against humanity on African slaves and their descendants." The hearing is scheduled to feature testimony from author Ta-Nehisi Coates, whose article "The Case for Reparations," published by The Atlantic magazine in 2014, is widely credited with re-igniting the

House Speaker Nancy Pelosi thinks the Democratic National Convention should be moved to a much bigger stadium so party faithful have space to social distance for Joe Biden's nomination. 

Genetic genealogy is a powerful crime-solving tool that combines DNA science with family tree research. Where will it take us – a crime-free world or a dark dystopia?

**
*Don’t miss out on the next big story. Get the Weekly Reveal newsletter today.*

This episode originally was broadcast Oct. 5, 2019.

Genetic genealogy is a powerful crime-solving tool that combines DNA science with family tree research. Where will it take us – a crime-free world or a dark dystopia?

Don’t miss out on the next big story. Get the Weekly Reveal newsletter today.

For latest iOS: If I'm actively using my phone, DND doesn't seem to work -- this is annoying when I'm trying to get lost in a book, long article, etc. Is there a way to force all incoming texts to obey DND at all times?

When I heard the news of John Prine's hospitalization on March 30, I was, of course, saddened. His music has meant so much to me over the decades. On that day, I recorded this cover of one of my favorites from Prine's early output, a political song cleverly disguised as a broken-hearted love ballad. There's a video I posted to YouTube of this very same performance, which also includes some spoken introduction, about how I was feeling upon getting the news of John's contracting the coronavirus, and so forth. So, if you'd like to hear that, and see the song performed, it's here. I did a little bit of work on the audio for this MeFiMu upload, though, so I think it might sound a little better here than on the video. Anyway, rest in peace, John Prine, rest in peace.

Upbeat and jangly with an orchestral edge. Trying to keep up with posting a song every month or so, this was recorded at the tail end of last summer.

When the British economy ground to a halt a few weeks ago, Reda Maher suddenly found himself among the ranks of the unemployed, alongside untold millions of other people around the world. But unlike many others, Maher can rest easy, knowing that money will keep flowing into his bank account until he's called back to work. "I woke up a couple of hours later than I normally would. I won't lie," Maher said one afternoon earlier this month. "I took a nice long masked and gloved walk. I've got a remote personal training like fitness session in about 20 minutes." The United Kingdom recently began paying 80% of the salaries of workers laid off because of the coronavirus pandemic. The government caps the pay at about $3,000 a month, but many employers, including the London-based video streaming service where Maher works, add to what the government hands out. Maher also doesn't need to worry about being left without health care coverage, thanks to Britain's National Health Service. Across