to write an operations manual template

End-to-end RNA-sequencing methods that capture 5'-sequence content without cumbersome library manipulations are of great interest, particularly for analysis of long RNAs. While template-switching methods have been developed for RNA sequencing by distributive short-read RTs, such as the MMLV RTs used in SMART-Seq methods, they have not been adapted to leverage the power of ultraprocessive RTs, such as those derived from group II introns. To facilitate this transition, we dissected the individual processes that guide the enzymatic specificity and efficiency of the multistep template-switching reaction carried out by RTs, in this case, by MarathonRT. Remarkably, this is the first study of its kind, for any RT. First, we characterized the nucleotide specificity of nontemplated addition (NTA) reaction that occurs when the RT extends past the RNA 5'-terminus. We then evaluated the binding specificity of specialized template-switching oligonucleotides, optimizing their sequences and chemical properties to guide efficient template-switching reaction. Having dissected and optimized these individual steps, we then unified them into a procedure for performing RNA sequencing with MarathonRT enzymes, using a well-characterized RNA reference set. The resulting reads span a six-log range in transcript concentration and accurately represent the input RNA identities in both length and composition. We also performed RNA-seq from total human RNA and poly(A)-enriched RNA, with short- and long-read sequencing demonstrating that MarathonRT enhances the discovery of unseen RNA molecules by conventional RT. Altogether, we have generated a new pipeline for rapid, accurate sequencing of complex RNA libraries containing mixtures of long RNA transcripts.

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Scientists have long debated how large-scale plant patterns such as the famous "fairy circles" of Namibia form and persist. Now, a new Princeton University-led study suggests that instead of a single overarching cause, large-scale vegetation patterns in arid ecosystems could occasionally stem from millions of local interactions among neighboring plants and animals. The work could explain many patterns throughout the world.

This year’s Mother’s Day has undoubtedly been impacted by the COVID-19 virus. Plans to show appreciation to the one you call ‘mama’ have been thwarted due to worldwide quarantine conditions. But while there may not be the usual elaborate dinner at...

The HIPAA Security & Privacy Compliance Toolkit (HSPCT) - Government Edition - is the most comprehensive set of Health Insurance Portability and Accountability Act (HIPAA) documents found anywhere in North America today.

The templates enable a holistic approach to analyzing data across marketing tools

A simple CSS ONLY modal windows template, suitable for all the latest browsers.

Hey Divi Nation! Thanks for joining us for the next installment of our weekly Divi Design Initiative where each week, we give away brand new freebies. This Monday, we shared a brand new Winery Layout Pack. To help you get your website up and running as soon as possible, we’re sharing a brand new blog […]

The post Get a FREE Blog Post Template for Divi’s Winery Layout Pack appeared first on Elegant Themes Blog.

Web clients love custom designs. Enough unique changes can turn a pre-made template into a beautiful, original site design. While there’s nothing wrong with tweaking a website for your clients, customization can be taken too far.   Granting excessive requests can put your projects into overtime and drive you mad. If you want to avoid […] More

Before 20 years, the websites are developed with text and static HTML only. But as technology is growing we have lots of new ways to create any complex to a simple website. There are so many options available to develop a user-friendly website.

This post outlines the steps I took to create a block-based theme version of Twenty Twenty. Thanks to Kjell Reigstad for helping develop the theme and write this post. There’s been a lot of conversation around how theme development changes as Full Site Editing using Gutenberg becomes a reality. Block templates are an experimental feature … Continue reading "Creating a Block-based Theme Using Block Templates"

Creating vintage style designs for company brands, T-shirts, packaging designs, book covers, window signs, and countless other graphic design project scenarios has never been easier with this huge bundle of 200 editable logo templates from Heritage Type Co. Logo Templates are ready-made designs that can be easily customised to quickly create stunning badges and emblems […]

The post All The Vintage Logo Template Designs You Could Ever Wish For! appeared first on Spoon Graphics.

Are you looking to build a new site for your business? If so, company website design templates can help you create the website you want. With so many templates available, how do you determine which one is best for your business? On this page, we’ll provide you with the top six company website design templates, […]

The post Top 6 Company Website Design Templates appeared first on WebFX Blog.

Welcome to the Design Patterns Demystified (DPD) series, in this edition we are going to discuss Template Design Pattern. So let us understand the why, how, what, and where of Template Design Pattern.

The Why

Let us understand first, why we need this pattern with the help of an example. Let's you are building a reusable library which is orchestrating the operation of buying an item on an e-commerce platform. Now, irrespective of what you are buying, you will follow the same sequence of steps like building your cart, adding an address, filling in payment details, and then finishing the payment. The details in these steps will vary based on what you are buying, how much you are buying, the delivery address, and the preferred mode of payment, but the complete orchestration of steps remains the same.

A system for blueprint-driven environment template creation in a virtual infrastructure comprises a processor and a memory. The processor is configured to receive a blueprint, receive an environment template configuration, and build an environment template using the blueprint and the environment template configuration. The environment template is for provisioning an environment. The environment is for deploying an application. The memory is coupled to the processor and is configured to provide the processor with instructions.

A system and methods for matching at least one word of an utterance against a set of template hierarchies to select the best matching template or set of templates corresponding to the utterance. Certain embodiments of the system and methods determines at least one exact, inexact, and partial match between the at least one word of the utterance and at least one term within the template hierarchy to select and populate a template or set of templates corresponding to the utterance. The populated template or set of templates may then be used to generate a narrative template or a report template.

Template and method of making high aspect ratio template, stamp, and imprinting at nanoscale using nanostructures for the purpose of lithography, and to the use of the template to create perforations on materials and products.

A method of making a metal oxide nanoparticle comprising contacting an aqueous solution of a metal salt with an oxidant. The method is safe, environmentally benign, and uses readily available precursors. The size of the nanoparticles, which can be as small as 1 nm or smaller, can be controlled by selecting appropriate conditions. The method is compatible with biologically derived scaffolds, such as virus particles chosen to bind a desired material. The resulting nanoparticles can be porous and provide advantageous properties as a catalyst.

Methods for creating customized children's storybooks with fingerprint art using fingerprint-ready templates stored in association with a software application on a desktop computer, a tablet computer, or a smart phone. The methods include the use of preconfigured templates and prompted discussions that elicit responses from the child to create text for the storybook. The text may be generated by speech recognition software processing digital audio recordings of the child's responses. A digital image of the child's fingerprint is acquired and the fingerprint image and/or the storybook scenes are scaled to fit each other. Fingerprint-ready image templates are used to facilitate the creation of fingerprint art. A completed page image is formed by digitally inserting a fingerprint into the fingerprint-shaped area, and if desired, coloring the image or drawing a scene around the image. When the scenes are completed the software application compiles the book pages and prints the same.

Systems and methods are provided for installing workflow servers for a print shop. One embodiment is a system that installs a workflow server for a print shop. The system accesses installation templates, configured for different categories of print shop, that describe a set of print shop resources expected to exist at the category of print shop. The system also installs workflow server software on a computer system of a print shop. During the installation, the system determines a category for the print shop, selects an installation template that matches the category of the print shop, and performs a dialogue of queries with a user to determine which resources of the set of print shop resources identified by the installation template are available to the print shop, wherein the dialogue excludes queries for print shop resources that are not in the set of print shop resources described by the installation template.

The present invention relates to an organotemplate-free synthetic process for the production of a zeolitic material comprising YO2 and X2O3, wherein said process comprises the steps of (1) preparing a mixture comprising seed crystals, one or more sources for YO2, one or more sources for X2O3, and one or more solvents;(2) crystallizing the mixture obtained in step (1) to obtain a zeolitic material comprising YO2 and X2O3 as a crystallization product; wherein Y is a tetravalent element, and X is a trivalent element, and wherein at least a portion of the mother liquor obtained in step (2) is recycled to step (1) as a source for YO2, optionally after concentration of the mother liquor.

The present invention relates to a system, method, and apparatus that include a novel way of automatically assigning materials to 3D CAD models. A predefined material template specifies that a particular part or material name is assigned to a particular material such that all instances of the particular part name are assigned or modified automatically by the material template. By having a consistent naming convention for the parts in the CAD file, this material assignment can be performed automatically when the CAD file is imported or viewed.

Techniques are presented for managing a deployment pipeline using an inheritable and extensible source code template—generally referred to as a live pipeline template (LPT). As described, live pipeline templates may be used to manage deployment pipelines which, in turn, are used to launch, maintain, and update the services and systems used to host and provide computing services.

Techniques are presented for managing a deployment pipeline using an inheritable and extensible source code template—generally referred to as a live pipeline template (LPT). As described, live pipeline templates may be used to manage deployment pipelines which, in turn, are used to launch, maintain, and update the services and systems used to host and provide computing services.

The present application provides a method and a device for extending a layout template of a component. The method includes determining an inheritance relationship between a component to be extended and an existing component, generating extension information of the component to be extended according to the inheritance relationship, where the extension information includes identification (ID) information of the existing component, the inheritance relationship, and content to be extended. The method further includes acquiring a layout template object of the existing component according to the ID information of the existing component, where the layout template object is configured with at least one element item of the corresponding component, and updating the element item in the layout template object according to the inheritance relationship and the content to be extended to obtain a layout template object. A layout template is extended using an inheritance relationship between objects to simplify the management of the layout templates and the complexity of maintenance and updates.

Methods for controlled microstructure creation utilize seeding of amorphous layers prior to annealing. Seed crystals are formed on an amorphous layer or layers. The material, size, and spacing of the seed crystals may be varied, and multiple seed layers and/or amorphous layers may be utilized. Thereafter, the resulting assembly is annealed to generate a crystalline microstructure. Via use of these methods, devices having desirable microstructural properties are enabled.

W.A. Production has launched a limited time offer on the Savage Bass House Bundle, a collection of sound packs, DAW templates and audio tutorial course at 95% off the regular price. You don’t have to go for broke to make great bass house. Our Savage Bass House Bundle comes complete with all the tools you […]

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