The ancient herb from the East meets modern vegan beauty technology, the new Red Ginseng skincare.

Serum proteomics reveal APOE-ε4 -dependent and APOE-ε4 -independent protein signatures in Alzheimer’s disease  Nature.com

M el-Saadani
Apr 1, 1989; 30:627-630
Articles

M. Burstein
Nov 1, 1970; 11:583-595
Articles

Oxygen regulates hypoxia-inducible factor (HIF) transcription factors to control cell metabolism, erythrogenesis, and angiogenesis. Whereas much has been elucidated about how oxygen regulates HIF, whether lipids affect HIF activity is un-known. Here, using cultured cells and two animal models, we demonstrate that lipoprotein-derived fatty acids are an independent regulator of HIF. Decreasing extracellular lipid supply inhibited HIF prolyl hydroxylation, leading to accumulation of the HIFα subunit of these heterodimeric transcription factors comparable with hypoxia with activation of downstream target genes. The addition of fatty acids to culture medium suppressed this signal, which required an intact mitochondrial respiratory chain. Mechanistically, fatty acids and oxygen are distinct signals integrated to control HIF activity. Finally, we observed lipid signaling to HIF and changes in target gene expression in developing zebrafish and adult mice, and this pathway operates in cancer cells from a range of tissues. This study identifies fatty acids as a physiological modulator of HIF, defining a mechanism for lipoprotein regulation that functions in parallel to oxygen.

Lipoprotein (a) [Lp(a)] is a well-known risk factor for cardiovascular disease, but analysis on Lp(a) and renal dysfunction is scarce. We aimed to investigate prospectively the association of serum Lp(a) with the risk of reduced renal function, and further investigated whether diabetic or hypertensive status modified such association. Six thousand two hundred and fifty-seven Chinese adults aged ≤40 years and free of reduced renal function at baseline were included in the study. Reduced renal function was defined as estimated glomerular filtration rate <60 ml/min/1.73 m². During a mean follow-up of 4.4 years, 158 participants developed reduced renal function. Each one-unit increase in log₁₀-Lp(a) (milligrams per deciliter) was associated with a 1.99-fold (95% CI 1.15–3.43) increased risk of incident reduced renal function; the multivariable-adjusted odds ratio (OR) for the highest tertile of Lp(a) was 1.61 (95% CI 1.03–2.52) compared with the lowest tertile (P for trend = 0.03). The stratified analysis showed the association of serum Lp(a) and incident reduced renal function was more prominent in participants with prevalent diabetes [OR 4.04, 95% CI (1.42–11.54)] or hypertension [OR 2.18, 95% CI (1.22–3.89)]. A stronger association was observed in the group with diabetes and high Lp(a) (>25 mg/dl), indicating a combined effect of diabetes and high Lp(a) on the reduced renal function risk. An elevated Lp(a) level was independently associated with risk of incident reduced renal function, especially in diabetic or hypertensive patients.

Coronavirus disease 2019 (COVID-19) is a highly contagious infection and threating the human lives in the world. The elevation of cytokines in blood is crucial to induce cytokine storm and immunosuppression in the transition of severity in COVID-19 patients. However, the comprehensive changes of serum proteins in COVID-19 patients throughout the SARS-CoV-2 infection is unknown. In this work, we developed a high-density antibody microarray and performed an in-depth proteomics analysis of serum samples collected from early COVID-19 (n = 15) and influenza (n = 13) patients. We identified a large set of differentially expressed proteins (n = 132) that participate in a landscape of inflammation and immune signaling related to the SARS-CoV-2 infection. Furthermore, the significant correlations of neutrophil and lymphocyte with the CCL2 and CXCL10 mediated cytokine signaling pathways was identified. These information are valuable for the understanding of COVID-19 pathogenesis, identification of biomarkers and development of the optimal anti-inflammation therapy.

Forever Youth Liberator Serum Video

Industry Experts at Truth in Aging awarded Best for Sagging Skin to Skin 2 Skin's Anti-Sagging Renewal Serum as Highly effective formula in firming sagging skin.

For the second year in a row Skin 2 Skin's Anti-Sagging Renewal Serum is awarded the coveted Truth In Aging's The Best Serum for Firming Sagging Skin, an extremely rare honor, it has to be said.

Industry Experts at Truth In Aging award Skin 2 Skin's hero product Anti-Sagging Renewal Serum 2 of the Best Product Awards for 2018 as a highly effective formula as Best Serum for Firming Sagging Skin and Community Favorite Best Anti-Aging Product.

Create faster and better routines with Serumize's new line of precision skincare products that target your unique skin concerns whilst challenging us to approach beauty from a functionality standpoint.

As usual, my dilemma for finding the perfect moisturizer has not yet solved. So, after using the hyaluronic acid serum (Marine Hyaluronics) from The Ordinary, this is my second hyaluronic acid serum and I was and was completely unsure of what to expect. The best part is I am writing the review almost a month […]

The post Dot & Key Water Drench Hydrating Hyaluronic Acid Serum Concentrate appeared first on Perfect Skin Care for you.

The invention relates to improved variants of the anti-serum albumin immunoglobulin single variable domain DOM7h-11, as well as ligands and drug conjugates comprising such variants, compositions, nucleic acids, vectors and hosts.

Arovane has announced the release of the Spectral 2 soundset, a fresh collection of 64 sounds for the popular Serum wavetable synthesizer by Xfer Records. The pack comes with 35 customized wavetables and 20 samples for the noise oscillator. Available for 19 EUR, Spectral 2 includes: 20 pads. 16 plucks. 13 soundscapes. 6 sweeps. 4 […]

The post Arovane releases Spectral 2 soundset for Xfer Serum synth appeared first on rekkerd.org.

ADSR Sounds has announced its latest sound pack hellofi, a collection of samples and Serum synth presets with an upbeat take on lo-fi and indie pop. Take a deep breath and lets the vibes hit you like sunlight through an open window. ADSR’s latest Indie Lo-Fi offering –hellofi, brings together a colourful collection of beats […]

The post ADSR Sounds launches hellofi sound pack incl. Serum presets appeared first on rekkerd.org.

Resonate Sound Design has announced the release of a new preset expansion pack for the popular Serum wavetable synthesizer by Xfer Records. Cyber delivers over 120 fresh presets. If you create trailer, cyberpunk, dark synthwave, darkwave or similar genre music, then this is the preset pack for you…not that you can’t use these for a […]

The post Resonate Sound Design releases Cyber soundset for Xfer Serum appeared first on rekkerd.org.

Hy2rogen has launched its new sound pack Bass House for Serum, a collection of handcrafted presets for the popular Serum software synthesizer. The pack includes 100 bass sounds for Bass House, Hybrid Trap, Dubstep, and more. It’s been a while since we’ve had some brand new presets over here at HY2ROGEN and now we’re back […]

The post Hy2rogen releases Bass House for Serum, Future House Drops & EDM Synth Shots 9 appeared first on rekkerd.org.

Loopmasters has announced its latest Patchworx series soundset Proxima Grime, a collection of 64 presets for the Serum wavetable synth by Xfer Records. This pack by Proxima features a selection of meticulously crafted heavy basses, twisted synths, dark pads, colorful FX and more. Proxima has been involved in music most of his life. His first […]

The post Loopmasters releases Proxima Grime sounds pack for Xfer Serum appeared first on rekkerd.org.

Datacode Records has announced the release of the new FOCUS: Serum Melodic Techno sound pack, a collection of Serum presets with a focus on Melodic Techno, Progressive House, Techno and Minimal. The pack features 68 brand new Serum presets and 20 Serum patches from FOCUS: Melodic Techno as a bonus. The follow up release to […]

The post FOCUS: Serum Melodic Techno sound pack by Datacode now available appeared first on rekkerd.org.

Plugin Boutique has launched a deal in which you can get the Light Serum Presets pack for free with a purchase of the Serum wavetable synthesizer by Xfer Records. The dream synthesizer did not seem to exist: a wavetable synthesizer with a truly high-quality sound, visual and creative workflow-oriented interface to make creating and altering […]

The post Get free Light Serum Presets with purchase of Xfer Serum synthesizer appeared first on rekkerd.org.

Ghosthack has launched an introductory sale on Analog Wavetables Vol. 2, offering a 67% discount on the collection of wavetables for the Serum synthesizer by Xfer Records. It is a hard task to create analog sounds in a digital environment. With the support of “Analog Wavetables for Serum Volume 2” it got much easier! As […]

The post Analog Wavetables Vol. 2 for Serum by Ghosthack on sale at 67% OFF appeared first on rekkerd.org.

Plugin Boutique has launched a sale on the Pumped: Serum Psytrance Essentials soundset by W.A. Production, offering a 50% discount on the collection of 90 presets for the Serum wavetable synthesizer by Xfer Records. Do you draw inspiration from such artists as Astrix, Astral Projection and Juno Reactor? Ever want the most cutting edge and […]

The post Pumped Serum Psytrance Essentials soundset by W.A. Production appeared first on rekkerd.org.

M el-Saadani
Apr 1, 1989; 30:627-630
Articles

M. Burstein
Nov 1, 1970; 11:583-595
Articles

Qingbo Shu
Apr 1, 2020; 19:672-689
Research

Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because of the wide dynamic range of serum protein abundances, the lack of a complete serum N-glycan database and the existence of proteoforms. In this regard, a spectral library search method was presented for the identification of N-linked intact glycopeptides from N-linked glycoproteins in human serum with target-decoy and motif-specific false discovery rate (FDR) control. Serum proteins were firstly separated into low-abundance and high-abundance proteins by acetonitrile (ACN) precipitation. After digestion, the N-linked intact glycopeptides were enriched by hydrophilic interaction liquid chromatography (HILIC) and a portion of the enriched N-linked intact glycopeptides were processed by Peptide-N-Glycosidase F (PNGase F) to generate N-linked deglycopeptides. Both N-linked intact glycopeptides and deglycopeptides were analyzed by LC-MS/MS. From N-linked deglycopeptides data sets, 764 N-linked glycoproteins, 1699 N-linked glycosites and 3328 unique N-linked deglycopeptides were identified. Four types of N-linked glycosylation motifs (NXS/T/C/V, X=P) were used to recognize the N-linked deglycopeptides. The spectra of these N-linked deglycopeptides were utilized for N-linked deglycopeptides library construction and identification of N-linked intact glycopeptides. A database containing 739 N-glycan masses was constructed and utilized during spectral library search for the identification of N-linked intact glycopeptides. In total, 526 N-linked glycoproteins, 1036 N-linked glycosites, 22,677 N-linked intact glycopeptides and 738 N-glycan masses were identified under 1% FDR, representing the most in-depth serum N-glycoproteome identified by LC-MS/MS at N-linked intact glycopeptide level.

Teng-Fei Yuan
Apr 1, 2020; 61:580-586
Methods

Sandi M. Azab
Mar 31, 2020; 0:jlr.D120000630v1-jlr.D120000630
Methods

Nutritional studies rely on various biological specimens for fatty acid (FA) determination, yet it is unclear how levels of serum non-esterified FA (NEFAs) correlate with other circulating lipid pools. Here, we used a high throughput method (< 4 min/sample) based on multisegment injection-non-aqueous-capillary electrophoresis–mass spectrometry (MSI-NACE-MS) to investigate whether specific serum NEFAs have utility as biomarkers of dietary fat intake in women. We first  identified circulating NEFAs correlated with long-term/habitual food intake among pregnant women with contrasting dietary patterns (n=50). Acute changes in serum NEFA trajectories were also studied in non-pregnant women (n=18) following high-dose (5 g/day) fish oil (FO) supplementation or isoenergetic sunflower oil placebo over 56 days. In the cross-sectional study, serum omega-3 (-3) FA correlated with self-reported total -3 daily intake, notably eicosapentaenoic acid (EPA) as its NEFA (r=0.46; p=0.001), whereas pentadecanoic acid was associated with full-fat dairy intake (r=0.43; p=0.002), outcomes consistent with results from  total FA serum hydrolysates. In the intervention cohort, serum -3 NEFAs increased 2.5-fold from baseline within 28 days following FO supplementation, and this increase was most pronounced for EPA (p=0.0004). Unlike for docosahexaenoic acid, circulating EPA as its NEFA also strongly correlated to EPA concentrations measured from erythrocyte phospholipid hydrolysates (r=0.66; p=4.6 x 10-10), and was better suited to detect dietary non-adherence. We conclude that MSI-NACE-MS offers a rapid method to quantify serum NEFAs and objectively monitor dietary fat intake in women that is complementary to diet records or food frequency questionnaires.

Liver-derived serum amyloid A (SAA) is present in plasma where it is mainly associated with HDL and from which it is cleared more rapidly than are the other major HDL-associated apolipoproteins. Although evidence suggests that lipid-free and HDL-associated forms of SAA have different activities, the pathways by which SAA associates and disassociates with HDL are poorly understood. In this study, we investigated SAA lipidation by hepatocytes and how this lipidation relates to the formation of nascent HDL particles. We also examined hepatocyte-mediated clearance of lipid-free and HDL-associated SAA. We prepared hepatocytes from mice injected with lipopolysaccharide or an SAA-expressing adenoviral vector. Alternatively, we incubated primary hepatocytes from SAA-deficient mice with purified SAA. We analyzed conditioned media to determine the lipidation status of endogenously produced and exogenously added SAA. Examining the migration of lipidated species, we found that SAA is lipidated and forms nascent particles that are distinct from apoA-I-containing particles and that apoA-I lipidation is unaltered when SAA is overexpressed or added to the cells, indicating that SAA is not incorporated into apoA-I-containing HDL during HDL biogenesis. Like apoA-I formation, generation of SAA-containing particles was dependent on ABCA1, but not on scavenger receptor class B type I. Hepatocytes degraded significantly more SAA than apoA-I. Taken together, our results indicate that SAA’s lipidation and metabolism by the liver is independent of apoA-I and that SAA is not incorporated into HDL during HDL biogenesis.

Analyzing global steroid metabolism in humans can shed light on the etiologies of steroid-related diseases. However, existing methods require large amounts of serum and lack the evaluation of accuracy. Here, we developed an LC/MS/MS method for the simultaneous quantification of 12 steroid hormones: testosterone, pregnenolone, progesterone, androstenedione, corticosterone, 11-deoxycortisol, cortisol, 17-hydroxypregnenolone, 17-hydroxyprogesterone, dehydroepiandrosterone, estriol, and estradiol. Steroids and spiked internal standards in 100 μl serum were extracted by protein precipitation and liquid-liquid extraction. The organic phase was dried by evaporation, and isonicotinoyl chloride was added for steroid derivatization, followed by evaporation under nitrogen and redissolution in 50% methanol. Chromatographic separation was performed on a reverse-phase PFP column, and analytes were detected on a triple quadrupole mass spectrometer with ESI. The lower limits of quantification ranged from 0.005 ng/ml for estradiol to 1 ng/ml for cortisol. Apparent recoveries of steroids at high, medium, and low concentrations in quality control samples were between 86.4% and 115.0%. There were limited biases (–10.7% to 10.5%) between the measured values and the authentic values, indicating that the method has excellent reliability. An analysis of the steroid metabolome in pregnant women highlighted the applicability of the method in clinical serum samples. We conclude that the LC/MS/MS method reported here enables steroid metabolome analysis with high accuracy and reduced serum consumption, indicating that it may be a useful tool in both clinical and scientific laboratory research.

Recent studies using mouse models suggest that interaction between the gut microbiome and IL-17/IL-22 producing cells plays a role in the development of metabolic diseases. We investigated this relationship in humans using data from the prediabetes study of the Integrated Human Microbiome Project (iHMP). Specifically, we addressed the hypothesis that early in the onset of metabolic diseases there is a decline in serum levels of IL-17/IL-22, with concomitant changes in the gut microbiome. Clustering iHMP study participants on the basis of longitudinal IL-17/IL-22 profiles identified discrete groups. Individuals distinguished by low levels of IL-17/IL-22 were linked to established markers of metabolic disease, including insulin sensitivity. These individuals also displayed gut microbiome dysbiosis, characterized by decreased diversity, and IL-17/IL-22-related declines in the phylum Firmicutes, class Clostridia, and order Clostridiales. This ancillary analysis of the iHMP data therefore supports a link between the gut microbiome, IL-17/IL-22 and the onset of metabolic diseases. This raises the possibility for novel, microbiome-related therapeutic targets that may effectively alleviate metabolic diseases in humans as they do in animal models.

Long-term hyperglycemia in patients with diabetes leads to human serum albumin (HSA) glycation, which may impair HSA function as a transport protein and affect the therapeutic efficacy of anticoagulants in patients with diabetes. In this study, a novel mass spectrometry approach was developed to reveal the differences in the profiles of HSA glycation sites between patients with diabetes and healthy subjects. K199 was the glycation site most significantly changed in patients with diabetes, contributing to different interactions of glycated HSA and normal HSA with two types of anticoagulant drugs, heparin and warfarin. An in vitro experiment showed that the binding affinity to warfarin became stronger when HSA was glycated, while HSA binding to heparin was not significantly influenced by glycation. A pharmacokinetic study showed a decreased level of free warfarin in the plasma of diabetic rats. A preliminary retrospective clinical study also revealed that there was a statistically significant difference in the anticoagulant efficacy between patients with diabetes and patients without diabetes who had been treated with warfarin. Our work suggests that larger studies are needed to provide additional specific guidance for patients with diabetes when they are administered anticoagulant drugs or drugs for treating other chronic diseases.

Massimo Cigolini
Mar 1, 2006; 29:722-724
BR Cardiovascular and Metabolic Risk

Earl S. Ford
May 1, 2005; 28:1228-1230
BR Metabolic Syndrome/Insulin Resistance Syndrome/Pre-Diabetes

Apr 1, 1994; 17:360-361
Syst[egrave]me International (SI) Units

Nov 1, 1995; 18:1524-1525
Syst[egrave]me International (SI) Units

Aug 1, 1995; 18:1223-1224
Syst[egrave]me International (SI) Units

Wien : Hartleben, 1901.

Halle : M. Niemeyer, 1895.

Leipzig : Wilhelm Engelmann, 1897.

Berlin : Heuser, 1894.