template Take Advantage of Excel Templates for Business, See These 15 Areas They Could Help By www.small-business-software.net Published On :: Mon, 27 May 2019 18:59:57 -0400 Spreadsheets have long been an important tool for businesses. You can use them for just about anything. This includes budgeting and planning out important marketing campaigns. No tool seems more synonymous with spreadsheets than Excel. Microsofts tool allows you to easily fill in spaces and create tables. But it also takes advantage of some more advanced features. However, starting from scratch will not give you a productive business. You may want to take advantage of all that Excel has to offer. But maybe you don’t know exactly how to get started. So templates become a great option. Check out some sources for Excel business templates that can help you in 15 different areas of business operations. complete article Full Article
template Change Normal Template in Libreoffice Writer By oksoft.antville.org Published On :: 2022-12-12T08:45:05Z Open a new file and set your font; Verdana; 18ptFile > Templates > Save as TemplateSelect > My Templates then tick the "Set as default template" boxEnter a name at the top then save and close the file.The next time you open Writer, the settings should be in place. Full Article
template link or unlink template By oksoft.antville.org Published On :: 2022-12-16T05:27:06Z If you are using Libre office then template changer extension is very important.extensions.libreoffice.orgYou can link the current document to a template or cut that link. Once the document is linked to a new template, all styles saved in that template will be available to be used. You can find the current template name by going to File - Properties. Full Article
template Templates in LO writer By oksoft.antville.org Published On :: 2024-10-09T08:07:35Z File > Templates > Save as TemplateGive it a name and select "My Templates".Tick the box Set as default template. Click SaveFile > Templates > Manage templates,Right click the template with a green tick beside it and select Reset Default. Full Article
template 20 Best Gaming HTML Website Templates By monsterspost.com Published On :: Fri, 19 Jul 2024 02:00:00 +0000 Whether you sell online games, have a game blog or work for a gaming company, our awesome collection of ready-made templates will be a great asset to you. The post 20 Best Gaming HTML Website Templates appeared first on MonstersPost. Full Article Theme Collections
template Three Column Blogger Template By bryanong.blogspot.com Published On :: Sat, 7 Jul 2007 02:02:00 +0930 This is by far the best site to download three column blogger template. It has all the blogger templates and it is really easy to install. Most of the sites I've visited have very limited templates or either have problems or makes it very difficult for a beginner to install. Check Webtalks blog out. Full Article blogger
template Text Template Parser 2.5 By www.template-parser.com Published On :: Tue, 22 Mar 2022 10:16:12 -0800 Text Template Parser is a data processing automation solution that allows to parse and extract data from any sort of documents, web pages, emails, orders, support enquiries or feedback forms. The software allows to print and save extracted information as XML, HTML or TXT document. All the mentioned features are provided with a user friendly graphical interface. Full Article
template Recommendation letter toolkit offering information, tips, and downloadable templates for writing all types of letters of recommendation for business, personal, and college admission purposes. By www.ebizindia.com Published On :: Mon, 13 Dec 2004 11:19:28 PST Recommendation Letter Toolkit That Is The One-And-Only Master Information Source For Writing All Types Of Letters Of Recommendation. If You Need To Write Any Kind of Recommendation Letter, or Letter Of Reference, Whether It's For Business, Work, College Admission, or Home, This Kit Is Your One-Stop Information Source. Full Article Home & Family -- Students & School
template New RSS2HTML Web Template Directory By www.feedforall.com Published On :: Tue, 15 Aug 2006 07:07:40 -0400 A new rss2html template directory has been announced by Notepage, Inc. The rss2html template directory will help webmasters control the appearance of the webpages that result when they use the free rss2html PHP script. The rss2html.php script converts RSS feeds to HTML. The rss2html templates are web templates that assists publishers in the layout of the resulting HTML when the feed is converted, so that they are assured a webpage that is graphically pleasing. The rss2html template directory has more than 400 templates with various colors and layouts. The templates can accommodate RSS feeds with images, text, audio, and video files. New templates will be added to the directory on a regular basis. The rss2html templates can also easily be integrated into an existing website's theme. The templates are standard HTML documents except they have special variables inserted where the RSS feed's data should appear. Users can purchase access to the rss2html template directory for a one time fee of $ 29.95. This subscription will allow for unlimited template usage. The fee also provides access to future templates added to the rss2html directory. Additional details and a preview of the directory can be found at http://www.feedforall.com/rss2html/index.htm . Full Article
template How To Write A Newsletter Toolkit: Copyright Free Articles, Newsletter Templates + 'How To' Manual By www.RSStop10.com Published On :: Mon, 03 Jan 2005 12:00:00 +0530 Stop Writing Newsletters The Hard Way! Here's How Anyone Can Turn Themselves Into An Award Winning Newsletter Editor! Discover How To Quickly and Easily Create Award Winning Newsletters In Less Than One Hour! Full Article
template SFIwebBuilder - Web Site Builder And Templates For SFI Affiliates By www.ebizindia.com Published On :: Thu, 13 Jan 2005 00:00:00 +0530 Get 20 Professionally designed website templates built from the ground up to help you grow your SFI business like the Pro's do! Create INSTANT web sites with SFIwebBuilder's web site building software Full Article Business -- Web Design
template RSS2HTML Templates By www.feedforall.com Published On :: Fri, 26 Aug 2016 09:00:00 -0400 The following are rss2html HTML templates that can be used in conjunction with the rss2html.php script to display RSS feeds as HTML web pages. RSS2HTML Templates Full Article
template Does Creative Suite Offer Free Templates for Commercial Use? By www.colourlovers.com Published On :: Fri, 27 Oct 2023 07:53:00 +0000 Are you looking for an online editor that offers free templates for commercial use? If so, you... Full Article __PRIMARY-CHANNELS _OTHER-CHANNELS Apps CHANNEL-DIGITAL-ART OTHER-DIGITAL-ART OTHER-WEB Software Tools Video
template Steal These Free Call Center Job Description Templates By www.quicksprout.com Published On :: Sun, 31 Mar 2024 16:00:00 +0000 Recruiting and hiring call center employees can be a pain, but it doesn’t have to be so bad. For example, depending on the roles you’re looking to hire, you may not even have to write a job description—because you can just use ours instead. Below, we’ve put together four of […] The post Steal These Free Call Center Job Description Templates appeared first on . Full Article Operations
template Modeling Quality Attributes with Aspect-Oriented Architectural Templates By www.jucs.org Published On :: 2011-05-06T16:03:16+02:00 The quality attributes of a software system are, to a large extent, determined by the decisions taken early in the development process. Best practices in software engineering recommend the identification of important quality attributes during the requirements elicitation process, and the specification of software architectures to satisfy these requirements. Over the years the software engineering community has studied the relationship between quality attributes and the use of particular architectural styles and patterns. In this paper we study the relationship between quality attributes and Aspect-Oriented Software Architectures - which apply the principles of Aspect-Oriented Software Development (AOSD) at the architectural level. AOSD focuses on identifying, modeling and composing crosscutting concerns - i.e. concerns that are tangled and/or scattered with other concerns of the application. In this paper we propose to use AO-ADL, an aspect-oriented architectural description language, to specify quality attributes by means of parameterizable, and thus reusable, architectural patterns. We particularly focus on quality attributes that: (1) have major implications on software functionality, requiring the incorporation of explicit functionality at the architectural level; (2) are complex enough as to be modeled by a set of related concerns and the compositions among them, and (3) crosscut domain specific functionality and are related to more than one component in the architecture. We illustrate our approach for usability, a critical quality attribute that satisfies the previous constraints and that requires special attention at the requirements and the architecture design stages. Full Article
template A Template-Based Short Course Concept on Android Application Development By Published On :: Full Article
template Generating a Template for an Educational Software Development Methodology for Novice Computing Undergraduates: An Integrative Review By Published On :: 2024-09-24 Aim/Purpose: The teaching of appropriate problem-solving techniques to novice learners in undergraduate software development education is often poorly defined when compared to the delivery of programming techniques. Given the global need for qualified designers of information technology, the purpose of this research is to produce a foundational template for an educational software development methodology grounded in the established literature. This template can be used by third-level educators and researchers to develop robust educational methodologies to cultivate structured problem solving and software development habits in their students while systematically teaching the intricacies of software creation. Background: While software development methodologies are a standard approach to structured and traceable problem solving in commercial software development, educational methodologies for inexperienced learners remain a neglected area of research due to their assumption of prior programming knowledge. This research aims to address this deficit by conducting an integrative review to produce a template for such a methodology. Methodology: An integrative review was conducted on the key components of Teaching Software Development Education, Problem Solving, Threshold Concepts, and Computational Thinking. Systematic reviews were conducted on Computational Thinking and Software Development Education by employing the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) process. Narrative reviews were conducted on Problem Solving and Threshold Concepts. Contribution: This research provides a comprehensive analysis of problem solving, software development education, computational thinking, and threshold concepts in computing in the context of undergraduate software development education. It also synthesizes review findings from these four areas and combines them to form a research-based foundational template methodology for use by educators and researchers interested in software development undergraduate education. Findings: This review identifies seven skills and four concepts required by novice learners. The skills include the ability to perform abstraction, data representation, decomposition, evaluation, mental modeling, pattern recognition, and writing algorithms. The concepts include state and sequential flow, non-sequential flow control, modularity, and object interaction. The teaching of these skills and concepts is combined into a spiral learning framework and is joined by four development stages to guide software problem solving: understanding the problem, breaking into tasks, designing, coding, testing, and integrating, and final evaluation and reflection. This produces the principal finding, which is a research-based foundational template for educational software development methodologies. Recommendations for Practitioners: Focusing introductory undergraduate computing courses on a programming syllabus without giving adequate support to problem solving may hinder students in their attainment of development skills. Therefore, providing a structured methodology is necessary as it equips students with essential problem-solving skills and ensures they develop good development practices from the start, which is crucial to ensuring undergraduate success in their studies and beyond. Recommendation for Researchers: The creation of educational software development methodologies with tool support is an under-researched area in undergraduate education. The template produced by this research can serve as a foundational conceptual model for researchers to create concrete tools to better support computing undergraduates. Impact on Society: Improving the educational value and experience of software development undergraduates is crucial for society once they graduate. They drive innovation and economic growth by creating new technologies, improving efficiency in various industries, and solving complex problems. Future Research: Future research should concentrate on using the template produced by this research to create a concrete educational methodology adapted to suit a specific programming paradigm, with an associated learning tool that can be used with first-year computing undergraduates. Full Article
template Intense Multipurpose Website Template: Technical Overview By www.templatemonster.com Published On :: Fri, 25 Oct 2019 12:38:44 +0000 Among web users, there is probably no one who’s never heard about the Intense theme. And all because it is a #1 best-selling website template on the TemplateMonster marketplace. Multipurpose, developed with users in mind, and up-to-date — these … The post Intense Multipurpose Website Template: Technical Overview appeared first on Template Monster Help. Full Article HTML Web Templates Website Builders Website Templates
template How to upload template (install) to Shopify By www.templatemonster.com Published On :: Fri, 03 Jan 2020 07:14:03 +0000 In this tutorial you will learn how to install Shopify template. The post How to upload template (install) to Shopify appeared first on Template Monster Help. Full Article Shopify Tutorials install Shopify template theme upload
template Shopify. How to create a page template By www.templatemonster.com Published On :: Fri, 03 Jan 2020 08:35:09 +0000 This tutorial provides an explanation of how to create a page template in Shopify. The post Shopify. How to create a page template appeared first on Template Monster Help. Full Article Shopify Tutorials create page Shopify template
template WordPress. How to install template over existing website By www.templatemonster.com Published On :: Mon, 27 Jan 2020 06:35:15 +0000 This tutorial is going to show you how to install a WordPress template on your existing website and make it look like the live demo preview. The post WordPress. How to install template over existing website appeared first on Template Monster Help. Full Article WordPress Tutorials existing install WordPress
template D8.1 Project branding (logo, flyer, PowerPoint and policy briefs templates), website, online libraries By www.eubon.eu Published On :: Mon, 09 Sep 2013 10:59:13 +0300 Full Article Events
template Paper provides safety ‘template’ for emerging technologies By www.safetyandhealthmagazine.com Published On :: Tue, 13 Aug 2024 00:00:00 -0400 Cincinnati — Drawing on the lessons learned from efforts to protect workers from the hazards associated with nanomaterials can help developers of new technologies ensure their safe use, researchers contend. Full Article
template Formation of extended polyiodides at large cation templates By journals.iucr.org Published On :: 2024-05-13 By studying the structures of (μ-1,4,10,13-tetrathia-7,16-diazacyclooctadecane)bis[iodidopalladium(II)] diiodide penta(diiodine), [Pd2I2(C12H26N2S4)](I)2·5I2 or [Pd2I2([18]aneN2S4)](I)2·(I2)5, and 4,7,13,16,21,24-hexaoxa-1,10-diazoniabicyclo[8.8.8]hexacosane triiodide iodide hemipenta(diiodine) dichloromethane monosolvate, C18H38N2O62+·I3−·I−·2.5I2·CH2Cl2 or [H2([2.2.2]cryptand)](I3)(I)(I2)2.5·CH2Cl2, we confirm the structural variety of extended polyiodides achievable upon changing the shape, charge and dimensions of the cation template, by altering the synthetic strategy adopted and/or the experimental conditions. Although it is still often difficult to characterize discrete [I2m+n]n− polyiodides higher than I3− on the basis of structural parameters, such as I—I bond distances, FT–Raman spectroscopy appears to identify them as aggregates of I2, I− and (symmetric or slightly asymmetric) I3− building blocks linked by I⋯I interactions of varying strengths. However, because FT–Raman spectroscopy carries no information about the topological features of extended polyiodides, the two techniques should therefore be applied in combination to enhance the analysis of this kind of compounds. Full Article text
template STOPGAP: an open-source package for template matching, subtomogram alignment and classification By journals.iucr.org Published On :: 2024-04-12 Cryo-electron tomography (cryo-ET) enables molecular-resolution 3D imaging of complex biological specimens such as viral particles, cellular sections and, in some cases, whole cells. This enables the structural characterization of molecules in their near-native environments, without the need for purification or separation, thereby preserving biological information such as conformational states and spatial relationships between different molecular species. Subtomogram averaging is an image-processing workflow that allows users to leverage cryo-ET data to identify and localize target molecules, determine high-resolution structures of repeating molecular species and classify different conformational states. Here, STOPGAP, an open-source package for subtomogram averaging that is designed to provide users with fine control over each of these steps, is described. In providing detailed descriptions of the image-processing algorithms that STOPGAP uses, this manuscript is also intended to serve as a technical resource to users as well as for further community-driven software development. Full Article text
template What shapes template-matching performance in cryogenic electron tomography in situ? By journals.iucr.org Published On :: 2024-05-28 The detection of specific biological macromolecules in cryogenic electron tomography data is frequently approached by applying cross-correlation-based 3D template matching. To reduce computational cost and noise, high binning is used to aggregate voxels before template matching. This remains a prevalent practice in both practical applications and methods development. Here, the relation between template size, shape and angular sampling is systematically evaluated to identify ribosomes in a ground-truth annotated data set. It is shown that at the commonly used binning, a detailed subtomogram average, a sphere and a heart emoji result in near-identical performance. These findings indicate that with current template-matching practices macromolecules can only be detected with high precision if their shape and size are sufficiently different from the background. Using theoretical considerations, the experimental results are rationalized and it is discussed why primarily low-frequency information remains at high binning and that template matching fails to be accurate because similarly shaped and sized macromolecules have similar low-frequency spectra. These challenges are discussed and potential enhancements for future template-matching methodologies are proposed. Full Article text
template Echo templates aid mental mapping in bats By esciencenews.com Published On :: Tue, 02 Aug 2016 18:04:57 +0000 A study published in eLife provides new insights on how bats recognise their surroundings to help them build mental maps. read more Full Article Mathematics & Economics
template MorganHill Launches Comprehensive ISO 27001 Risk Assessment Template to Support ISO/IEC 27001:2022 Certification Process By www.24-7pressrelease.com Published On :: Wed, 11 Sep 2024 08:00:00 GMT MorganHill is proud to announce the release of its new ISO 27001 Risk Assessment Template, designed to assist organizations in navigating the complexities of the ISO/IEC 27001:2022 certification process. Full Article
template Arlington Announces Availability of All-New NIST 800-171 System Security Plan (SSP), Version 3 Template for Immediate Download By www.24-7pressrelease.com Published On :: Thu, 08 Aug 2024 08:00:00 GMT Arlington, a leading provider of compliance solutions and cybersecurity expertise, is excited to announce the release of its all-new NIST 800-171 System Security Plan (SSP), Version 3. Full Article
template Square Signs Reveals the Most Popular Sign Templates Used by SMEs in Winter 2024 By www.24-7pressrelease.com Published On :: Tue, 05 Mar 2024 08:00:00 GMT Square Signs reveals a list of templates that have been heavily used by SMEs this winter. Customers often employ templates that are simple and practical. Full Article
template MorganHill Launches Essential ISO 27001 Internal Audit Template for Instant Download for Achieving ISO 2700 Certification By www.24-7pressrelease.com Published On :: Thu, 29 Aug 2024 08:00:00 GMT ISO 27001 certification is a critical benchmark for organizations looking to enhance their information security practices and demonstrate their commitment to protecting sensitive data. Full Article
template MorganHill Launches ISO 27001 Risk Assessment Template to Simplify ISO 27001 Certification Process By www.24-7pressrelease.com Published On :: Thu, 29 Aug 2024 08:00:00 GMT MorganHill is excited to announce the release of its innovative ISO 27001 Risk Assessment Template, designed to streamline the process of achieving ISO 27001 certification for organizations of all sizes. Full Article
template MorganHill Unveils ISO 27001 Risk Assessment Template for Instant Download to Streamline Security Risk Management By www.24-7pressrelease.com Published On :: Fri, 30 Aug 2024 08:00:00 GMT MorganHill, a pioneering leader in information security solutions, is excited to announce the release of its ISO 27001 Risk Assessment Template, now available for instant download. Full Article
template MorganHill Announces Launch of Comprehensive ISO 27001 Policy Templates to Enhance Data Security By www.24-7pressrelease.com Published On :: Fri, 30 Aug 2024 08:00:00 GMT MorganHill's commitment to advancing information security is reflected in the high standards and meticulous attention to detail embodied in these new templates. Full Article
template Streamline Your ISO 27001 Audit: MorganHill's New ISO 27001 Internal Audit Template Now Available for Immediate Download By www.24-7pressrelease.com Published On :: Wed, 04 Sep 2024 08:00:00 GMT MorganHill, a premier provider of ISO 27001 consulting services, is excited to unveil its new ISO 27001 Internal Audit Template, now available for immediate download. Full Article
template MorganHill Announces Availability of ISO 27001 Policy Templates to Simplify Compliance By www.24-7pressrelease.com Published On :: Thu, 05 Sep 2024 08:00:00 GMT The ISO 27001 Policy Templates are available for immediate download on MorganHill's website and are designed to be customizable to fit the specific needs of various organizations. Full Article
template Square Signs Template Page Upgrades to Help SMEs Save Time on Business Prints By www.24-7pressrelease.com Published On :: Tue, 05 Sep 2023 08:00:00 GMT Square Signs introduces their upgraded sign templates with new features and layouts. The company shares their crowd-pleasing template designs that are in high demand by business owners. Full Article
template 30 Awesome Latest Free High Quality Web Templates By sharebrain.info Published On :: Fri, 03 Aug 2012 14:06:00 +0000 Hello friends, I have compiled some of the beautiful web templates, now if you want to design a website you can probably use these templates, this will help you to reduce your designing work. Thanks to the original designers for sharing such wonderful templates for free.View and Vote Full Article Freebies
template 45 High Quality And Free Web Templates In PSD By sharebrain.info Published On :: Fri, 03 Aug 2012 20:59:00 +0000 Finding out high quality and premium PSD files is extremely difficult but keeping them in the toolbox is tremendously important for the web designers because such PSD files always come in handy and save loads of time.View and Vote Full Article Photoshop
template ETSI releases a standard for NFV Deployment Templates By www.etsi.org Published On :: Thu, 28 Apr 2022 13:58:19 GMT ETSI releases a standard for NFV Deployment Templates Sophia Antipolis, 30 January 2019 It provides the foundations of an open ecosystem The ETSI Industry Specification Group (ISG) on Network Functions Virtualisation (NFV) ended 2018 on a bright note, with the publication of the first version of ETSI GS NFV-SOL 001, the specification of NFV descriptors based on the Topology and Orchestration Specification for Cloud Applications (TOSCA). Read More... Full Article
template CVE-2024-50386: Apache CloudStack: Directly downloaded templates can be used to abuse KVM-based infrastructure By seclists.org Published On :: Tue, 12 Nov 2024 14:25:57 GMT Posted by Daniel Augusto Veronezi Salvador on Nov 12Severity: important Affected versions: - Apache CloudStack 4.0.0 through 4.18.2.4 - Apache CloudStack 4.19.0.0 through 4.19.1.2 Description: Account users in Apache CloudStack by default are allowed to register templates to be downloaded directly to the primary storage for deploying instances. Due to missing validation checks for KVM-compatible templates in CloudStack 4.0.0 through 4.18.2.4 and 4.19.0.0 through 4.19.1.2, an attacker that... Full Article
template [ M.3020 (2011) Amendment 1 (07/14) ] - Indication of naming attribute in analysis template By www.itu.int Published On :: Tue, 25 Nov 2014 15:53:00 GMT Indication of naming attribute in analysis template Full Article
template [ D.170 (2010) Supplement 4 (01/12) ] - ITU-T D.170 - Supplement on guidelines for net settlement generic templates By www.itu.int Published On :: Fri, 22 Jun 2012 11:05:00 GMT ITU-T D.170 - Supplement on guidelines for net settlement generic templates Full Article
template ADCS Cert Templates for ISE Lab By lostintransit.se Published On :: Tue, 22 Oct 2024 06:15:06 +0000 In my ISE lab I’m going to be using EAP-TLS and TEAP, which means I’ll be needing user and computer certificates. The goal is to be able to enable the 802.1X supplicant via GPO and to distribute certificates automatically without Full Article ISE ADCS Certificate Template
template How to Render Templates in Flask By www.hongkiat.com Published On :: Tue, 22 Oct 2024 13:00:06 +0000 Flask is a lightweight web framework for Python that makes it easy to build web applications. In our previous article, we’ve seen how to set up a simple page. But, it’s just a simple text like “Hello Flask!”. In real applications, you’ll want to render more than just simple text. You’ll want to render HTML… The post How to Render Templates in Flask appeared first on Hongkiat. Full Article Coding
template Slices template By www.chathamhouse.org Published On :: Fri, 11 Sep 2020 08:56:32 +0000 Slices template Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. nfaulds-adams… 11 September 2020 Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum. Full Article
template Polymerase {gamma} efficiently replicates through many natural template barriers but stalls at the HSP1 quadruplex [Enzymology] By www.jbc.org Published On :: 2020-12-18T00:06:18-08:00 Faithful replication of the mitochondrial genome is carried out by a set of key nuclear-encoded proteins. DNA polymerase γ is a core component of the mtDNA replisome and the only replicative DNA polymerase localized to mitochondria. The asynchronous mechanism of mtDNA replication predicts that the replication machinery encounters dsDNA and unique physical barriers such as structured genes, G-quadruplexes, and other obstacles. In vitro experiments here provide evidence that the polymerase γ heterotrimer is well-adapted to efficiently synthesize DNA, despite the presence of many naturally occurring roadblocks. However, we identified a specific G-quadruplex–forming sequence at the heavy-strand promoter (HSP1) that has the potential to cause significant stalling of mtDNA replication. Furthermore, this structured region of DNA corresponds to the break site for a large (3,895 bp) deletion observed in mitochondrial disease patients. The presence of this deletion in humans correlates with UV exposure, and we have found that efficiency of polymerase γ DNA synthesis is reduced after this quadruplex is exposed to UV in vitro. Full Article
template Amplification and Identification of Vertebrate Host Cytochrome c Oxidase Subunit I (COI) DNA Barcoding Templates from Mosquito Blood Meals By cshprotocols.cshlp.org Published On :: 2024-10-01T07:08:54-07:00 Mosquitoes take blood meals from a diverse range of host animals and their host associations vary by species. Characterizing these associations is an important element of the transmission dynamics of mosquito-vectored pathogens. To characterize mosquito host associations, various molecular techniques have been developed, which are collectively referred to as blood meal analysis. DNA barcoding has diverse biological applications and is well-suited to mosquito blood meal analysis. The standard DNA barcoding marker for animals is a 5' fragment of the cytochrome c oxidase I (COI) gene. A major advantage of this marker is its taxonomic coverage in DNA sequence reference databases, making it feasible to identify a wider range of mosquito host species than with any other gene. However, the COI gene contains high sequence variation at potential priming sites between vertebrate orders. Coupled with the need for primer sequences to be mismatched with mosquito priming sites so that annealing to mosquito DNA is inhibited, it can be difficult to design primers suitable for blood meal analysis applications. Several primers are available that perform well in mosquito blood meal analysis, annealing to priming sites for most vertebrate host taxa, but not to those of mosquitoes. Because priming site sequence variation among vertebrate taxa can cause amplification to fail, a hierarchical approach to DNA barcoding-based blood meal analysis can be applied. In such an approach, no single primer set is expected to be effective for 100% of potential host species. If amplification fails in the initial reaction, a subsequent reaction is attempted with primers that anneal to different priming sites, and so on, until amplification is successful. Full Article
template PHP Project Template By www.phpclasses.org Published On :: Wed, 06 Nov 2024 19:52:07 GMT Package: PHP Project Template Summary: Generate files for PHP projects from templates Groups: Code Generation, PHP 7, Project Management Author: Eric Sizemore Description: This package can generate files for PHP projects from templates... Read more at https://www.phpclasses.org/package/13386-PHP-Generate-files-for-PHP-projects-from-templates.html#2024-11-06-11:52:07 Full Article
template to write policy and procedures manual template By english.al-akhbar.com Published On :: to write policy and procedures manual template Full Article