Labor shortages continue to pressure manufacturers, with some dedicating up to 20% of their workforce to manual inspection. Embracing Quality 4.0 with automated in-line inspections and AI process analytics could provide significant value.

Investigative journalist Arun Gupta offers an eyewitness account of the hate—and sense of belonging—on display at Donald Trump’s New York City rally.

Manufacturing

Mikayla Cluff, Haley McCutcheon, and Adriana scored for coach Seb Hines' team.

Kimberly Cheatle, who had served as Secret Service director since August 2022, faced growing calls to resign.

The post Secret Service director steps down after assassination attempt against ex-President Trump at rally first appeared on Federal News Network.

Dana Laake and her special guest Dr. Véronique Desaulniers will discuss her book, Heal Breast Cancer Naturally: 7 Essential Steps to Beating Breast Cancer.

The post Heal breast cancer naturally, 7 essential steps to beating breast cancer first appeared on Federal News Network.

Hundreds of far-right protesters, some dressed in goggles, gas masks and knee pads, are rallying in support of Ann Coulter as dozens of police in riot gear stand ready to crack down on violence if it erupts

The post The Latest: Ann Coulter a no-show at rally in Berkeley first appeared on Federal News Network.

Hundreds of people chanting "USA" gathered peacefully for a rally at a park in Berkeley _ home of the free speech movement _ to protest a canceled appearance by conservative commentator Ann Coulter

The post Berkeley protests peaceful as hundreds rally over Coulter first appeared on Federal News Network.

What does fried chicken and notebooks have in common? Read to find out.

Stocks rally and euro tumbles as Trump declares victory in the US election

The left tries to find its footing before Trump takes power again. by Hannah Krieg

Photography by Ananya Mishra

Seattle lefties gathered at the Space Needle this weekend to hype themselves up for what promises to be a rough four years under a second Trump administration. Speakers acknowledged that the future feels foggy, and that it's unclear what the first fights will be.

Unlike many of the protests around the country, Seattle’s wasn’t organized by the Women’s March or driven by Trump’s attacks on abortion, women, and LGBTQ rights. But it was an important opportunity for local activists to start saying some of our new realities out loud: that we’re about to have a president that seems determined to level Gaza, deport both documented and undocumented immigrants, undermine worker power, and continue to allow cops to kill with impunity. 

Perhaps unsurprisingly, though, not everyone left feeling hyped. The protest lacked the same energy that they harnessed in the wake of Trump’s election eight years ago, or even some of the other protests around the country this weekend. The calls to action—“resist, organize, fight”—felt too vague, especially when the left has had so little success organizing on the national stage. “It felt like we went from resistance to resignation and that this was just perfunctory," one attendee said. 

But we’re also only six days into this new paradigm. And we captured what promises to be the first protest of many as Seattle’s left finds their footing, and their energy. 

marusin posted a photo:

via Instagram ift.tt/2lQp78j

Live updates from former President Donald Trump's rally in Aurora today. Speakers have included U.S. Rep. Lauren Boebert and state Rep. Gabe Evans, a congressional candidate.

Live updates from former President Donald Trump's rally in Aurora today. Speakers have included U.S. Rep. Lauren Boebert and state Rep. Gabe Evans, a congressional candidate.

When the Nuggets are right, they play a beautiful game. They cut to the hoop, make deft passes, play tenacious defense, and hit the open shot.

As money continues to pour into crypto following Donald Trump’s victory last week, bitcoin has climbed to yet another record high.

Queen Elizabeth wore a neon green outfit to her 90th birthday party. What could go wrong? Oh right, the internet...

Investing.com-- Bitcoin fell from record highs on Wednesday, retreating as optimism over a Donald Trump presidency now appeared to be cooling, with focus turning to upcoming U.S. inflation data for more cues. Major meme token Dogecoin also retreated on Wednesday after a stellar run-up to…

Asia-Pacific stock markets were trading mostly lower Wednesday, tracking losses on Wall Street as the U.S. post election rally stalled overnight. Asian traders assessed corporate goods data out of Japan, which showed year-on-year producer price growth, or wholesale inflation, in October reached…

A surge in Bitcoin has paused as traders assess the remaining market impact of President-elect Donald Trump’s rhetorical support for crypto. Most Read from Bloomberg The digital asset is up about 30% in the wake of Trump’s Nov. 5 election victory, hitting a record of $89,968 on Tuesday, based on…

Investing.com-- Most Asian stocks fell on Wednesday, tracking losses in Wall Street as a post-election rally now appeared to be cooling, while anticipation of key U.S. inflation data also spurred risk aversion. Regional markets remained under pressure after fresh fiscal measures from China largely…

The action really picks up around the thirty second mark.

Respect for Badminton: +1

My boss is a seventy-something-year-old man with barely a clue on how to get a computer to boot. He gave me an old book and wanted me to convert it into an ebook to sell. And mind, this was a thick, large-format book chock-full of maps and other illustrations with tiny blackletter script.

Me: “Sure, I can do it, but I’d have to scan it in a massive resolution so the detail isn’t lost. The final file would be massive; it wouldn’t be practical to download it, and a normal ebook reader wouldn’t be able to display it correctly.”

Boss: “So, we’d have to make it less detailed.”

Me: “How do you mean?”

Boss: “It wouldn’t be possible with the illustrations; you’ll just have to make the writing bigger on all the pages.”

Me: “…”

Boss: “As for the pages with only text on them, you will just convert them into a Word document.”

Me: “That’s not how that works.”

Boss: “Why not?”

Me: “It’s just straight-up not possible, at least not with the software we have.”

Boss: “Can you do it on the Internet?”

Me: “No.”

Boss: “How do you know?”

Me: “I know.”

Boss: “Show me.”

I showed him that it’s not possible to convert a scanned book page into a text document on some random converter found on page one on Google.

Boss: “Okay, so you will instead cut the text out in Photoshop, make it larger, and arrange it on a new Photoshop file the same size, with less of a rim around it so the number of pages doesn’t get much higher.”

I flat-out refused, telling him it would be months of absolutely pointless work. He didn’t believe any of my claims, anyway, so I just converted the whole d*** thing into an ebook, which, in the end, was like 8GB in size. Since our server had 10TB, he also didn’t believe me when I tried to tell him that it was an absurdly massive file that few people would want to buy on that account.

Ah, well. At least I didn’t have to rearrange like 300 pages of text.

Bond yields spiked aggressively as investors awaited Wednesday's inflation data for October.

Bitcoin has been reaching new all-time highs almost daily, fueling a parabolic bull run that’s pushing various cryptocurrencies to surge… Continue reading Flockerz Capitalizes on Bitcoin Rally, Raising $1.6M in Presale

The post Flockerz Capitalizes on Bitcoin Rally, Raising $1.6M in Presale appeared first on ReadWrite.

There are a few moments in this race that historians will wrangle over as the 'real' reason Kamala lost. There are a couple of key events that are sure to make the list.

The post The RIGHT Rally: On Election Night, MAGA Takes Moment To Honor God Beautifully appeared first on Clash Daily.

Heterotrimeric G-proteins are signaling switches broadly divided into four families based on the sequence and functional similarity of their Gα subunits: Gs, Gi/o, Gq/11, and G12/13. Artificial mutations that activate Gα subunits of each of these families have long been known to induce oncogenic transformation in experimental systems. With the advent of next-generation sequencing, activating hotspot mutations in Gs, Gi/o, or Gq/11 proteins have also been identified in patient tumor samples. In contrast, patient tumor-associated G12/13 mutations characterized to date lead to inactivation rather than activation. By using bioinformatic pathway analysis and signaling assays, here we identified cancer-associated hotspot mutations in Arg-200 of Gα13 (encoded by GNA13) as potent activators of oncogenic signaling. First, we found that components of a G12/13-dependent signaling cascade that culminates in activation of the Hippo pathway effectors YAP and TAZ is frequently altered in bladder cancer. Up-regulation of this signaling cascade correlates with increased YAP/TAZ activation transcriptional signatures in this cancer type. Among the G12/13 pathway alterations were mutations in Arg-200 of Gα13, which we validated to promote YAP/TAZ-dependent (TEAD) and MRTF-A/B-dependent (SRE.L) transcriptional activity. We further showed that this mechanism relies on the same RhoGEF-RhoGTPase cascade components that are up-regulated in bladder cancers. Moreover, Gα13 Arg-200 mutants induced oncogenic transformation in vitro as determined by focus formation assays. In summary, our findings on Gα13 mutants establish that naturally occurring hotspot mutations in Gα subunits of any of the four families of heterotrimeric G-proteins are putative cancer drivers.

Chorismate mutase (CM), an essential enzyme at the branch-point of the shikimate pathway, is required for the biosynthesis of phenylalanine and tyrosine in bacteria, archaea, plants, and fungi. MtCM, the CM from Mycobacterium tuberculosis, has less than 1% of the catalytic efficiency of a typical natural CM and requires complex formation with 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase for high activity. To explore the full potential of MtCM for catalyzing its native reaction, we applied diverse iterative cycles of mutagenesis and selection, thereby raising kcat/Km 270-fold to 5 × 105 m−1s−1, which is even higher than for the complex. Moreover, the evolutionarily optimized autonomous MtCM, which had 11 of its 90 amino acids exchanged, was stabilized compared with its progenitor, as indicated by a 9 °C increase in melting temperature. The 1.5 Å crystal structure of the top-evolved MtCM variant reveals the molecular underpinnings of this activity boost. Some acquired residues (e.g. Pro52 and Asp55) are conserved in naturally efficient CMs, but most of them lie beyond the active site. Our evolutionary trajectories reached a plateau at the level of the best natural enzymes, suggesting that we have exhausted the potential of MtCM. Taken together, these findings show that the scaffold of MtCM, which naturally evolved for mediocrity to enable inter-enzyme allosteric regulation of the shikimate pathway, is inherently capable of high activity.

21 August 2020

Viral infection is one environmental factor that may contribute to the initiation of pancreatic β-cell destruction during the development of autoimmune diabetes. Picornaviruses, such as encephalomyocarditis virus (EMCV), induce a pro-inflammatory response in islets leading to local production of cytokines, such as IL-1, by resident islet leukocytes. Furthermore, IL-1 is known to stimulate β-cell expression of iNOS and production of the free radical nitric oxide. The purpose of this study was to determine whether nitric oxide contributes to the β-cell response to viral infection. We show that nitric oxide protects β-cells against virally mediated lysis by limiting EMCV replication. This protection requires low micromolar, or iNOS-derived, levels of nitric oxide. At these concentrations nitric oxide inhibits the Krebs enzyme aconitase and complex IV of the electron transport chain. Like nitric oxide, pharmacological inhibition of mitochondrial oxidative metabolism attenuates EMCV-mediated β-cell lysis by inhibiting viral replication. These findings provide novel evidence that cytokine signaling in β-cells functions to limit viral replication and subsequent β-cell lysis by attenuating mitochondrial oxidative metabolism in a nitric oxide–dependent manner.

Natural compounds that can stimulate salivary secretion are of interest in developing treatments for xerostomia, the perception of a dry mouth, that affects between 10 and 30% of the adult and elderly population. Chemesthetic transient receptor potential (TRP) channels are expressed in the surface of the oral mucosa. The TRPV1 agonists capsaicin and piperine have been shown to increase salivary flow when introduced into the oral cavity but the sialogogic properties of other TRP channel agonists have not been investigated. In this study we have determined the influence of different TRP channel agonists on the flow and protein composition of saliva. Mouth rinsing with the TRPV1 agonist nonivamide or menthol, a TRPM8 agonist, increased whole mouth saliva (WMS) flow and total protein secretion compared with unstimulated saliva, the vehicle control mouth rinse or cinnamaldehyde, a TRPA1 agonist. Nonivamide also increased the flow of labial minor gland saliva but parotid saliva flow rate was not increased. The influence of TRP channel agonists on the composition and function of the salivary proteome was investigated using a multi-batch quantitative MS method novel to salivary proteomics. Inter-personal and inter-mouth rinse variation was observed in the secreted proteomes and, using a novel bioinformatics method, inter-day variation was identified with some of the mouth rinses. Significant changes in specific salivary proteins were identified after all mouth rinses. In the case of nonivamide, these changes were attributed to functional shifts in the WMS secreted, primarily the over representation of salivary and nonsalivary cystatins which was confirmed by immunoassay. This study provides new evidence of the impact of TRP channel agonists on the salivary proteome and the stimulation of salivary secretion by a TRPM8 channel agonist, which suggests that TRP channel agonists are potential candidates for developing treatments for sufferers of xerostomia.

Thousands of teachers will head to the state capital on Wednesday to call for a nearly $10,000 raise over four years and an increase to per-pupil spending.

Leon Draisaitl had two goals, including the overtime winner, and Connor McDavid had a goal and three assists to move one point shy of 1,000 for his career as the Edmonton Oilers beat the New York Islanders 4-3 on Tuesday night.

More than 50 Norman Wells residents gathered at the Royal Canadian Legion on Thursday night to voice frustrations over high heating fuel prices.

Tens of thousands of Poles including nationalist opposition chiefs walked through Warsaw on Monday in an annual Independence Day event held by the far right, some shooting red flares and carrying anti-EU, anti-Ukraine and white supremacist banners.

Bitcoin prices have passed a record $84,000 in what could be the start of its post-halving rally after the presidential elections last week.

The comments come at a time when more foreign-born black people live in the United States than at any time in history—and many of the residents are children enrolled in the nation's K-12 public schools.

Division of Civil Rights and Public Trust is prosecuting the case Following an investigation by the Division of Civil Rights and Public Trust, a New Castle County grand jury charged a Dover man with multiple felonies for removing and pointing a handgun toward others while he participated in a counter-protest on September 24, 2020.  Michael […]

DELHI — A surge in Bitcoin has paused as traders assess the remaining market impact of United States President-elect Donald Trump's rhetorical support for crypto.

Title: Report: Vaccines Generally Safe, Cause Few Health Problems
Category: Health News
Created: 8/26/2011 11:01:00 AM
Last Editorial Review: 8/26/2011 12:00:00 AM

Title: Scientists Unravel Secrets of People Who Naturally Suppress HIV
Category: Health News
Created: 8/26/2020 12:00:00 AM
Last Editorial Review: 8/27/2020 12:00:00 AM

BACKGROUND:Practice on fasting prior to extubation in critically ill patients is variable. Efficacy of fasting in reducing gastric volume has not been well established. The primary objective of this study was to assess the effect of 4 h of fasting on prevalence of empty stomach using gastric ultrasonography in critically ill subjects who are fasted for extubation. The secondary objectives were to evaluate the change in gastric volumes during 4 h of fasting and to determine factors associated with empty stomach after fasting.METHODS:This was a single-center, prospective, observational study on adult ICU subjects who were enterally fed for at least 6 h continuously and mechanically ventilated. Gastric ultrasound was performed immediately prior to commencement of fasting, after 4 h of fasting, and after nasogastric (NG) aspiration after 4 h of fasting. An empty stomach was defined as a gastric volume ≤ 1.5 mL/kg.RESULTS:Forty subjects were recruited, and 38 (95%) had images suitable for analysis. The prevalence of empty stomach increased after 4 h of fasting (25 [65.8%] vs 31 [81.6%], P = .041) and after 4 h of fasting with NG aspiration (25 [65.8%] vs 34 [89.5%], P = .008). There was a significant difference in median (interquartile range) gastric volume per body weight between before fasting and 4 h after fasting (1.0 [0.5–1.8] mL/kg vs 0.4 [0.2–1.0] mL/kg, P < .001). No patient factors were associated with higher prevalence of empty stomach after 4 h of fasting.CONCLUSIONS:Most mechanically ventilated subjects had empty stomachs prior to fasting for extubation. Fasting for 4 h further increased the prevalence of empty stomach at extubation to > 80%.

CNN anchor Anderson Cooper was flabbergasted Wednesday by a surrogate’s defense of Donald Trump, calling his explanation for the former president’s bombastic statements literal “bulls---.”

On AC360, former California Lieutenant Gov. Abel Maldonado, a Republican, said that Trump’s recent comments calling for the military to “handle” Democrats were simply his way of expressing his inner New Yorker. “He’s a fighter,” Maldonado added.

The conversation began when Cooper brought up Trump’s former chairman of the Joint Chiefs of Staff, Mark Milley, who, alongside other military leaders, have begun to warn of the dangers of re-electing the former president. Milley has called Trump a “fascist to his core.”

Read more at The Daily Beast.

Stocks have soared since Donald Trump was elected, but some say the run-up has more to do with bubbles than policy

Playtonic Friends just announced that Victory Heat Rally from developer Skydevilpalm is coming to mobile through Crunchyroll and also on …

Continue reading "‘Victory Heat Rally’ From Skydevilpalm and Playtonic Friends Is Coming to PC and Crunchyroll for Mobile on October 3rd"

SOME volunteer firefighters feel left in the dark after the Country Fire Authority’s board and chief executive were replaced within 24 hours.

hello products are a solution to the struggle at the sink. This advertisement shows the frustrations moms and dads can face when it�s brush time and they need to negotiate with their kids to brush.