"Death, Instead of Cartier" - “Octopussy” tribute artwork by Gerald Wadsworth

Issue 4 of "Layered Butter" - a magazine dedicated to cinema and alternative art. This issue is about James Bond.

I've never quite understood the concept of "James Bond Day" (or "Global James Bond Day?"). But maybe that's because since I was 11, I don't think there's been any day I haven't thought about James Bond! Maybe there are people out there who need reminding? Anyway, to mark this year's James Bond Day, MGM and EON have released the first poster for Daniel Craig's final outing as Bond, No Time To Die. No Time To Die, directed by Cary Joji Fukunaga and starring Craig, Rami Malek, Léa Seydoux, Lashana Lynch, Ana de Armas, Jeffrey Wright, Ralph Fiennes, Naomie Harris, Ben Whishaw, Billy Magnussen, David Dencik, and Rory Kinnear, opens in the U.S. on April 8, 2020.

It's here! The trailer we've been waiting so long for! And our first lengthy look Daniel Craig in action as James Bond since SPECTRE in 2015. (I'm a little surprised at how direct a sequel to that movie No Time To Die appears to be.) Check it out:

Wow! We're so close to the release of a new Bond movie now that a new James Bond theme song has been released into the world! Listen for yourself to Billie Eilsish's title track to the twenty-fifth EON 007 movie, No Time to Die. Eilish recently won all the Grammies, pretty much, and performed at the Oscars. It seems pre-ordained that this track will shoot to the top of the charts. Eilish reportedly wrote the song with her brother, Finneas. Hans Zimmer composed the film's score.

This week MGM has ramped up the campaign for Daniel Craig's long awaited and highly anticipated fifth James Bond movie, No Time To Die. And as fans are painfully aware, this is actually the film's second campaign. No Time To Die had a whole advertising campaign back at the beginning of this year leading up to a hoped for April release, including many posters and even the much publicized, chart-topping release of the movie's excellent theme song by Billie Eilish. But, as we all know, the global pandemic came along and the April release was scrapped. Since then the film has been set for a November release, though even that has been uncertain given the unpredictable nature of the novel coronavirus. Now it certainly looks like EON, MGM, and distributors United Artists and Universal are doubling down on that November release! The date is proudly proclaimed across this brand new poster (frankly a little uninspired compared to the less typical previous campaign's 1-sheet) and in the brand new second trailer. And it's quite a trailer! As far as I can recall, this marks the first time a Craig Bond movie has had this kind of text tagline: The mission that changes everything begins! 

No sooner has Anthony Horowitz completed his trilogy of excellent, period-set James Bond novels than the next iteration of Ian Fleming Publications' literary continuations appears on the horizon via HarperCollins: Double or Nothing, by Kim Sherwood. And this one forsakes 007 (for the moment, anyway) to put the crosshairs on 003, 004, and 009... and the whole Double O Section! It should be unsurprising, given the name of this site, that I find that prospect tantalizing. Since I was a kid, I've been very curious to read about the adventures of the other 00 agents! 

Usually when we meet them in the movies, they're already dead or just about to die. The only other active agents we've ever really gotten to know well were Suzie Kew and Briony Thorne in Jim Lawrence's Daily Express James Bond comic strip, Nomi in No Time to Die... and I suppose we ought to also count Scarlett Papava in Sebastian Faulks's thoroughly disappointing Devil May Care. (Read my review here.)

Now, I know. You might ask, "What's the point of a James Bond continuation novel without 007?" To which I would point out that this has actually worked very successfully in the past! Some of my very favorite Bond continuation novels ever are Kate Westbrook's (aka Samantha Weinberg's) Moneypenny Diaries trilogy. (Read my review of the second one, Secret Servant, here, and read my in-depth interview with Weinberg about writing the series here.) Weinberg put the spotlight on Moneypenny, and created thrilling and original narratives in the familiar setting of Fleming's Secret Service. 

Kim Sherwood has already demonstrated her bona fides in her Twitter feed and on her website, and it sounds like she knows her spy stuff. (Not only is she well versed in Fleming, but she's also a Modesty Blaise fan!) I can't wait to see what she does with Fleming's supporting characters and the new 00 agents she creates in her Double O Section trilogy! The first book, Double or Nothing, is due out September 1 in Britain. A signed edition with stenciled page edges is also available exclusively from Waterstones (pictured below). No U.S. publication date has been announced so far, but Sherwood recently hinted on Twitter that such an announcement might be imminent. 

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“Chalk Talk” returns to talk about student health, school bonds and more. A new analysis looks at California’s campaign watchdog agency. Finally, Sacramento ghost guides talk about some of their favorite supernatural tales.

By Manola Secaira

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Despite its major importance in human health, the metabolic potential of the human gut microbiota is still poorly understood. We have recently shown that biosynthesis of Ruminococcin C (RumC), a novel ribosomally synthesized and posttranslationally modified peptide (RiPP) produced by the commensal bacterium Ruminococcus gnavus, requires two radical SAM enzymes (RumMC1 and RumMC2) catalyzing the formation of four Cα-thioether bridges. These bridges, which are essential for RumC's antibiotic properties against human pathogens such as Clostridium perfringens, define two hairpin domains giving this sactipeptide (sulfur-to-α-carbon thioether–containing peptide) an unusual architecture among natural products. We report here the biochemical and spectroscopic characterizations of RumMC2. EPR spectroscopy and mutagenesis data support that RumMC2 is a member of the large family of SPASM domain radical SAM enzymes characterized by the presence of three [4Fe-4S] clusters. We also demonstrate that this enzyme initiates its reaction by Cα H-atom abstraction and is able to catalyze the formation of nonnatural thioether bonds in engineered peptide substrates. Unexpectedly, our data support the formation of a ketoimine rather than an α,β-dehydro-amino acid intermediate during Cα-thioether bridge LC–MS/MS fragmentation. Finally, we explored the roles of the leader peptide and of the RiPP precursor peptide recognition element, present in myriad RiPP-modifying enzymes. Collectively, our data support a more complex role for the peptide recognition element and the core peptide for the installation of posttranslational modifications in RiPPs than previously anticipated and suggest a possible reaction intermediate for thioether bond formation.

Diana Samodova
Dec 1, 2020; 19:2139-2156
Technological Innovation and Resources

Trypsin is the protease of choice in bottom-up proteomics. However, its application can be limited by the amino acid composition of target proteins and the pH of the digestion solution. In this study we characterize ProAlanase, a protease from the fungus Aspergillus niger that cleaves primarily on the C-terminal side of proline and alanine residues. ProAlanase achieves high proteolytic activity and specificity when digestion is carried out at acidic pH (1.5) for relatively short (2 h) time periods. To elucidate the potential of ProAlanase in proteomics applications, we conducted a series of investigations comprising comparative multi-enzymatic profiling of a human cell line proteome, histone PTM analysis, ancient bone protein identification, phosphosite mapping and de novo sequencing of a proline-rich protein and disulfide bond mapping in mAb. The results demonstrate that ProAlanase is highly suitable for proteomics analysis of the arginine- and lysine-rich histones, enabling high sequence coverage of multiple histone family members. It also facilitates an efficient digestion of bone collagen thanks to the cleavage at the C terminus of hydroxyproline which is highly prevalent in collagen. This allows to identify complementary proteins in ProAlanase- and trypsin-digested ancient bone samples, as well as to increase sequence coverage of noncollagenous proteins. Moreover, digestion with ProAlanase improves protein sequence coverage and phosphosite localization for the proline-rich protein Notch3 intracellular domain (N3ICD). Furthermore, we achieve a nearly complete coverage of N3ICD protein by de novo sequencing using the combination of ProAlanase and tryptic peptides. Finally, we demonstrate that ProAlanase is efficient in disulfide bond mapping, showing high coverage of disulfide-containing regions in a nonreduced mAb.

Nov. 12—Jarrett Gronski wasn't expecting to cry the morning before playing Wayne State. Like most athletes, the Bemidji State football team's senior running back has his pregame rituals. But that routine shook out a little differently before the Beavers' game against the Wildcats. John Gronski — Jarrett's father and a prolific running back for BSU back in the late 1980s — was officially ...

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Todd Colvin, Ambrosino Brothers

Todd Colvin, Ambrosino Brothers

Larry Shover, Efficient Advisors

Dan Deming, KKM Financial

Dan Deming, KKM Financial

Todd Colvin, Ambrosino Brothers

In the 1980s, two babies were named after OM’s second ship. Both young men are now living out the ethos of the ministry, as servants of Christ.

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