Escalation in protests across the globe in 2019 are forecast to persist into the new decade, according to Verisk Maplecroft report.

Pennsylvania is promoting a new roadmap to electrify transportation by designing policies and setting targets to get more electric vehicles on the roads.

A new career map, Careers in Climate Control Technology, provides a first-of-its-kind interactive, visual tool to showcase the employment opportunities that exist in the swiftly growing HVAC/R industry – Heating, Ventilation, Air Conditioning and Refrigeration. The sector is on track for projected growth of 15 percent from 2016 to 2026.

A new roadmap for the future of Ohio’s electric grid can benefit all types of interest groups, but the next few years will be critical for the plan to achieve its goals.

On June 8, during its annual forum held in Ottawa, Ontario, Canada, the Canadian Hydropower Association announced the availability of an online map that depicts the country’s hydropower resources. Developed in coordination with the Royal Canadian Geographical Society (RCGS), CHA said the map is a tool to explore the story of Canadian hydropower development and learn about one of Canada’s greatest resources.
 

A new career map, Careers in Climate Control Technology, provides a first-of-its-kind interactive, visual tool to showcase the employment opportunities that exist in the swiftly growing HVAC/R industry – Heating, Ventilation, Air Conditioning and Refrigeration. The sector is on track for projected growth of 15 percent from 2016 to 2026.

Curbing global warming emissions and building the clean energy economy are critical to New York State’s future and the health and well-being of all New Yorkers. In this week’s State of the State event, Governor Cuomo announced some significant new clean energy and climate initiatives.

Researchers at Yale have unveiled a new interactive map that estimates public opinion on global warming right down to the county level.

By Chris Mitchell, vice president, global government relations As the unprecedented COVID-19 crisis continues to unfold, IPC is calling for a bold, sustained policy agenda to help the electronics manufacturing sector weather the economic downturn and support the economic recovery. There can be no doubt that governments all over the world should take extraordinary measures […]

Pennsylvania is promoting a new roadmap to electrify transportation by designing policies and setting targets to get more electric vehicles on the roads.

France will not make a decision on holidays until early June - while Germany is warning against a "race to allow tourism first", and some smaller EU states, such as Greece, Denmark and Austria, are considering allowing foreign holidays.

After a 15-year study, an international team of astronomers has unveiled a map with the highest resolution to date of the spiral structure of the Milky Way, offering clear proof that it is a barred spiral

...

President Cyril Ramaphosa has authorised the parole of selected low-risk prisoners to alleviate pressure at correctional services facilities amid the coronavirus pandemic.

The Mental Health Association, a Turkish-based non-governmental organization, has found the Maiden Tower in Istanbul is where the users of a smartphone application found the most romantic place in Istanbul, Turkey’s largest city.

The end of December 2019 saw the next step in the EU’s commitment to the adoption of a circular economy as the EU Commission published a road map on a new action plan which aims to speed up the transition to a circular economy. The new plan is...

The NBA has been in shutdown since the global pandemic brought sport to a standstill.

1

1

Countries can minimize the economic risk of epidemics by investing in the tools needed to predict disease emergence.

The “Equal Earth” projection shows the true area of continents such as Africa without greatly distorting their shapes and is already being adopted by NASA

The planet’s largest areas of undisturbed wilderness in Siberia and tropical rainforests are under threat from huge waves of development. Here’s what it looks like

Interstellar dust has been found in Antarctic snow samples. The discovery could provide a way of mapping the clouds of dust Earth has passed through in space

A new consensus document provides guidance on the safe reintroduction of cardiovascular procedures and testing derailed by the COVID-19 pandemic.

Title: Could AI Help Doctors Map Out Treatments for Brain Cancers?
Category: Health News
Created: 4/24/2020 12:00:00 AM
Last Editorial Review: 4/27/2020 12:00:00 AM

ABSTRACT

Gram-negative bacteria are intrinsically resistant to many antibiotics due to their outer membrane barrier. Although the outer membrane has been studied for decades, there is much to uncover about the biology and permeability of this complex structure. Investigating synthetic genetic interactions can reveal a great deal of information about genetic function and pathway interconnectivity. Here, we performed synthetic genetic arrays (SGAs) in Escherichia coli by crossing a subset of gene deletion strains implicated in outer membrane permeability with nonessential gene and small RNA (sRNA) deletion collections. Some 155,400 double-deletion strains were grown on rich microbiological medium with and without subinhibitory concentrations of two antibiotics excluded by the outer membrane, vancomycin and rifampin, to probe both genetic interactions and permeability. The genetic interactions of interest were synthetic sick or lethal (SSL) gene deletions that were detrimental to the cell in combination but had a negligible impact on viability individually. On average, there were ~30, ~36, and ~40 SSL interactions per gene under no-drug, rifampin, and vancomycin conditions, respectively; however, many of these involved frequent interactors. Our data sets have been compiled into an interactive database called the Outer Membrane Interaction (OMI) Explorer, where genetic interactions can be searched, visualized across the genome, compared between conditions, and enriched for gene ontology (GO) terms. A set of SSL interactions revealed connectivity and permeability links between enterobacterial common antigen (ECA) and lipopolysaccharide (LPS) of the outer membrane. This data set provides a novel platform to generate hypotheses about outer membrane biology and permeability.

IMPORTANCE Gram-negative bacteria are a major concern for public health, particularly due to the rise of antibiotic resistance. It is important to understand the biology and permeability of the outer membrane of these bacteria in order to increase the efficacy of antibiotics that have difficulty penetrating this structure. Here, we studied the genetic interactions of a subset of outer membrane-related gene deletions in the model Gram-negative bacterium E. coli. We systematically combined these mutants with 3,985 nonessential gene and small RNA deletion mutations in the genome. We examined the viability of these double-deletion strains and probed their permeability characteristics using two antibiotics that have difficulty crossing the outer membrane barrier. An understanding of the genetic basis for outer membrane integrity can assist in the development of new antibiotics with favorable permeability properties and the discovery of compounds capable of increasing outer membrane permeability to enhance the activity of existing antibiotics.

Site-specific recombinases, such as Cre, are a widely used tool for genetic lineage tracing in the fields of developmental biology, neural science, stem cell biology, and regenerative medicine. However, nonspecific cell labeling by some genetic Cre tools remains a technical limitation of this recombination system, which has resulted in data misinterpretation and led to many controversies in the scientific community. In the past decade, to enhance the specificity and precision of genetic targeting, researchers have used two or more orthogonal recombinases simultaneously for labeling cell lineages. Here, we review the history of cell-tracing strategies and then elaborate on the working principle and application of a recently developed dual genetic lineage-tracing approach for cell fate studies. We place an emphasis on discussing the technical strengths and caveats of different methods, with the goal to develop more specific and efficient tracing technologies for cell fate mapping. Our review also provides several examples for how to use different types of DNA recombinase–mediated lineage-tracing strategies to improve the resolution of the cell fate mapping in order to probe and explore cell fate–related biological phenomena in the life sciences.

Select agents (SA) pose unique challenges for licensing vaccines and therapies. In the case of toxin-mediated diseases, HHS assigns guidelines for SA use, oversees vaccine and therapy development, and approves animal models and approaches to identify mechanisms for toxin neutralization. In this commentary, we discuss next-generation vaccines and therapies against ricin toxin and botulinum toxin, which are regulated SA toxins that utilize structure-based approaches for countermeasures to guide rapid response to future biothreats.

RiVax is a promising recombinant ricin toxin A subunit (RTA) vaccine antigen that has been shown to be safe and immunogenic in humans and effective at protecting rhesus macaques against lethal-dose aerosolized toxin exposure. We previously used a panel of RTA-specific monoclonal antibodies (MAbs) to demonstrate, by competition enzyme-linked immunosorbent assay (ELISA), that RiVax elicits similar serum antibody profiles in humans and macaques. However, the MAb binding sites on RiVax have yet to be defined. In this study, we employed hydrogen exchange-mass spectrometry (HX-MS) to localize the epitopes on RiVax recognized by nine toxin-neutralizing MAbs and one nonneutralizing MAb. Based on strong protection from hydrogen exchange, the nine MAbs grouped into four spatially distinct epitope clusters (namely, clusters I to IV). Cluster I MAbs protected RiVax's α-helix B (residues 94 to 107), a protruding immunodominant secondary structure element known to be a target of potent toxin-neutralizing antibodies. Cluster II consisted of two subclusters located on the "back side" (relative to the active site pocket) of RiVax. One subcluster involved α-helix A (residues 14 to 24) and α-helices F-G (residues 184 to 207); the other encompassed β-strand d (residues 62 to 69) and parts of α-helices D-E (154 to 164) and the intervening loop. Cluster III involved α-helices C and G on the front side of RiVax, while cluster IV formed a sash from the front to back of RiVax, spanning strands b, c, and d (residues 35 to 59). Having a high-resolution B cell epitope map of RiVax will enable the development and optimization of competitive serum profiling assays to examine vaccine-induced antibody responses across species.

A logistic regression model and a bivariate statistical analysis were used in this paper to evaluate the groundwater recharge susceptibility. The approach is based on the assessment of the relationship involving groundwater recharge and parameters that influence this hydrological process. Surface parameters and aquifer-related parameters were evaluated as thematic map layers using ArcGIS. Then, a weighted-rating method was adopted to categorize each parameter's map. To assess the role of each parameter in the aquifer recharge, a logistic regression model and a bivariate statistical analysis were applied to the Guenniche phreatic aquifer (Tunisia). Models are explored to establish a map showing the aquifer recharge susceptibility. The code Modflow was used to simulate the consequence of the recharge. The recharge amount was introduced in the model and was tested to verify the recharge effect on the hydraulic head for the two models. The obtained results reveal that the recharge as mapped in the bivariate statistical model has a minor impact on the hydraulic head. Results of the logistic regression model are more significant as the hydraulic head is widely affected. This model provides good results in mapping the spatial distribution of the aquifer recharge susceptibility.

In recent years, there have been intensive debates on whether healthy adults acquire new word knowledge through fast mapping (FM) by a different mechanism from explicit encoding (EE). In this study, we focused on this issue and investigated to what extent retention interval, prior knowledge (PK), and lure type modulated memory after FM and EE. Healthy young participants were asked to learn novel word-picture associations through both FM and EE. Half of the pictures were from familiar categories (i.e., high PK) and the other half were from unfamiliar categories (i.e., low PK). After 10 min and 1 wk, the participants were tested by forced-choice (FC) tasks, with lures from different categories (Experiment 1) or from the same categories of the target pictures (Experiment 2). Pseudowords were used to denote names of the novel pictures and baseline performance was controlled for each task. The results showed that in both Experiments 1 and 2, memory performance remained stable after FM, while it declined after EE from 10 min to 1 wk. Moreover, the effect of PK appeared at 10 min after FM while at 1 wk after EE in Experiment 2. PK enhanced memory of word-picture associations when the lures were from the same categories (Experiment 2), rather than from different categories (Experiment 1). These results were largely confirmed in Experiment 3 when encoding condition was manipulated as a between-subjects factor, while lure type as a within-subjects factor. The findings suggest that different from EE, FM facilitates rapid acquisition and consolidation of word-picture knowledge, and highlight that PK plays an important role in this process by enhancing access to detailed information.

A new method called MicroMapping can identify nanoscale protein–protein interactions on live cells.

As part of its eventual launch on Steam, daunting simulation Dwarf Fortress has been planning a big visual overhaul. “Now with graphics!” Bay 12 proclaim on the store page. In a new little update, they’re giving us a look at what the generated world maps will look like with these non-ASCII pixels—though, yes, you’ll still […]

More than 3.9 million people have been sickened and more than 270,000 killed by the coronavirus around the world. This map is updated daily.

Destiny 2 Trials of Osiris returns once again later today – here's what you need to know for this week's end-game PvP challenge.

Need help tracking down and completing all 120 shrines in the Legend of Zelda: Breath of the Wild? Look no further. Here's everything you need to know, from the complete list of shrines to a map with every shrine location.

This interactive map for Assassin's Creed Odyssey will help you find everything you need, like ainigmata ostraka and tombs.

BORIS JOHNSON will give garden centres the go-ahead to reopen from next Wednesday in one of the first steps out of the coronavirus lockdown, Downing Street sources told the Daily Express on Friday night.

THE UK reached a grim milestone today as the total number of coronavirus deaths is believed to have surpassed 36,000.

A new global simulation model offers the first long-term look at how urbanization -- the growth of cities and towns -- will unfold in the coming decades. The research team projects the total amount of urban areas on Earth can grow anywhere from 1.8 to 5.9-fold by 2100, building approximately 618,000 square miles.

Follow our live updates here Coronavirus: The symptoms

The latest countries and areas affected by coronavirus Coronavirus: The symptoms

The Irish Government has shared it plans for reopening society after coronavirus lockdown.

The first changes to the UK's coronavirus lockdown will be "small" and "very carefully monitored" when the Prime Minister reveals his "roadmap" on Sunday.

No longer able to walk hundreds of miles to create his hand-drawn maps, the Beijing-based artist Fuller is charting his thoughts – and the global crisis – while stuck indoors

I’m interested in the psychology of a place and how it makes you feel. So I don’t really see myself as a map-maker – even though I draw maps. It’s about the process of travelling through a place to capture a sense of it. First, I walk for hundreds of miles and make all sorts of notes, and then take thousands of photos to use as triggers for my memory.

I walked more than 840 miles around Beijing when researching the map that became part of my Purposeful Wandering series. I circumnavigated the city and then walked around each ring road. Beijing (where I’ve lived for three years) is built on a Central Axis, and the map is, too. A lot of the drawing is literal, but I also built in personal experiences, references, visual puns and semiotics.