Here are the intraday supports and resistances for widely traded stocks such as Reliance Industries, ITC, ONGC, Infosys, HDFC Bank, TCS, and SBI

In 2016 the morning after the election I went to work early. Looking back at my camera roll from that day, here’s Batman, with a reminder. DON’T BELIEVE THOSE CRACKPOT LIES ABOUT PEOPLE WHO WORSHIP DIFFERENTLY, OR WHOSE SKIN IS OF A DIFFERENT COLOR, OR WHOSE PARENTS COME FROM ANOTHER COUNTRY. REMEMBER OUR AMERICAN HERITAGE...

It took me some time to learn this, but in all you do
in your prospecting efforts there is a much
better way than following a cold calling script and
putting undue pressure on both yourself and your
prospect for the close and it's much easier than you
think.

Once I really understood the power of doing this one
thing my business took off.

Here it is.

Instead of going for the hard sell, BE what your
prospects are looking for.

Very subtle what I just said, but more powerful than
you can imagine.

Instead of reading a script and getting nervous about
whether or not you're going to close that
particular lead into your business shoot right on
past it. Start training them from the outset.

What are you as a sponsor?

A coach that is going to help your prospect get to
where they want to go faster and easier than if
they tried on their own, so my philosophy is this . . .

Start teaching and training your would be recruit
BEFORE they are a recruit and just watch what
happens.

They won't know what's happening, but you'll be
attracting them towards like a magnet INSTEAD of
forcing them into your business with all your might
with the hard sell.

Speak to your prospect about what you do, give them
advice, steer them in the right direction WHILE
they are still prospects and watch how naturally most
will want to BE a part of whatever it is you're
doing.


What if you could literally leapfrog over your
competition and attract prospects to you seemingly
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Resistance bands for strength training and a video on lateral shoulder raises exercise as demonstrated by Kim Lyons .

Stock markets ended sharply lower today, marking the fifth straight session of declines as concerns around rising inflation and a broad selloff in metal stocks dragged sentiment.

Both the Nifty and Sensex recorded their steepest fall in five months, with the Nifty closing down 1.3 per cent, or 312 points, at 23,571.

The Sensex tumbled 1,125 points, or 1.4 per cent, to settle at 77,550.

Fr. Pat discusses the patterns of thought that make man so resistant to the cross.

The Cross is the key to unlocking God’s will for us in every stage of our lives. But at every stage, we may find ourselves resistant to the word of the Cross. And just when we imagine we have grasped what it means to be a Christian, we discover, perhaps with shock, that we’ve hardly begun.

The decades long battle between a small Scottish community and Donald Trump's golf dream

The incoming Trump administration has a message for left-leaning Justice Department employees hoping to block or interfere with the new president's agenda: Get out.

Fire resistance codes play in important factor in the design of buildings.

I have two glove samples for my cut hazard. One is an ASTM cut level 4 and the other is labeled EN cut level 5. Which glove is more cut-resistant?

Can you explain the misconception of EN vs. ASTM cut standards?

In the recent edition of NFPA 70E, high-resistance grounding is recognized as an arc flash reduction technology. In what way does employing HRG technology address the concern regarding arc flash?

What is really driving this boom is the way these products and networks are starting to play together, allowing people to add what they want with whatever system they already have and control everything with one app.

Sherwin-Williams High Performance Flooring launched Accelera One is an advanced coating technology designed for decorative flake and quartz flooring systems that meet the rigorous demands of commercial and institutional environments, delivering a high level of performance, ease of application and chemical resistance.

Affilia, Ahead of Print. This study intervenes in a growing scholarly dialogue about neoliberalism in social work and social welfare by addressing the undertheorized concept of resistance. We conduct a scoping review of 54 articles published from 2008–2023 to answer two questions: how is resistance discussed in relation to neoliberalism, and what are the practice […]

The post The Nexus of Resistance and Neoliberalism in Social Work and Social Welfare: A Scoping Review was curated by information for practice.

Vancomycin is a glycopeptide antibiotic that for decades has been a mainstay of treatment for persistent bacterial infections. However, the spread of antibiotic resistance threatens its continued utility. In particular, vancomycin-resistant enterococci (VRE) have become a pressing clinical challenge. Vancomycin acts by binding and sequestering the intermediate Lipid II in cell-wall biosynthesis, specifically recognizing a d-alanine-d-alanine dipeptide motif within the Lipid II molecule. VRE achieve resistance by remodeling this motif to either d-alanine-d-lactate or d-alanine-d-serine; the former substitution essentially abolishes recognition by vancomycin of Lipid II, whereas the latter reduces the affinity of the antibiotic by roughly one order of magnitude. The complex of vancomycin bound to d-alanine-d-serine has been crystallized, and its 1.20 Å X-ray crystal structure is presented here. This structure reveals that the d-alanine-d-serine ligand is bound in essentially the same position and same pose as the native d-alanine-d-alanine ligand. The serine-containing ligand appears to be slightly too large to be comfortably accommodated in this way, suggesting one possible contribution to the reduced binding affinity. In addition, two flexible hydroxyl groups – one from the serine side chain of the ligand, and the other from a glucose sugar on the antibiotic – are locked into single conformations in the complex, which is likely to contribute an unfavorable entropic component to the recognition of the serine-containing ligand.

Bacteria that resist antibiotics can be passed from food animals to humans, but not enough is known to determine the public health risks posed by such transmission, says a new report by a committee of the National Research Council.

Young athletes in the U.S. face a culture of resistance to reporting when they might have a concussion and to complying with treatment plans.

Safe, effective antimicrobial medicines are essential to modern medical procedures and pandemic preparedness. A new National Academies report recommends a range of policy, regulatory, and financial actions to tackle antimicrobial resistance, spanning human, animal, and environmental health.

Certain antibiotic resistance genes are easily transferred from one bacterial species to another, and can move between farm animals and the human gut. A team led by Chinese researchers has characterized this "mobile resistome," which they say is largely to blame for the spread of antibiotic resistance. They found that many antibiotic resistance genes that are shared between the human and animal gut microbiome are also present in multiple human pathogens. These findings are published September 9 in Applied and Environmental Microbiology, a journal of the American Society for Microbiology.

read more

In a creative stroke inspired by Hollywood wizardry, scientists from Harvard Medical School and Technion-Israel Institute of Technology have designed a simple way to observe how bacteria move as they become impervious to drugs.

read more

The Plastics Pipe Institute (PPI) published a new technical report related to the break resistance of PEX pipe and tubing when fluid inside becomes frozen.

T.U.B.E (The Ultimate Band Experience) TRAINING uses tube resistance bands to leverage the many planes of motion the human body moves through - not just the vertical plane defined by gravity. T.U.B.E TRAINING can be done indoors or outdoors.

T.U.B.E TRAINING™️ offers a Flexible Solution for Individuals Looking to Move Their Workouts Outdoors

For a Tax-Free Green New Deal

Our immune systems generate antibodies that neutralize and help eliminate foreign pathogens. These antibodies or proteins are generated by specific immune

A study recently published in Cell Metabolism examines the effects of intermittent fasting (IF) on neuronal insulin resistance (IR) and cognitive function

A study recently published in Cell Metabolism examines the effects of intermittent fasting (IF) on neuronal insulin resistance (IR) and cognitive function

Our immune systems generate antibodies that neutralize and help eliminate foreign pathogens. These antibodies or proteins are generated by specific immune

Our immune systems generate antibodies that neutralize and help eliminate foreign pathogens. These antibodies or proteins are generated by specific immune

Exploring protein relative relations in skeletal muscle proteomic analysis for insights into insulin resistance and type 2 diabetes  Nature.com

Technicians need to understand the basic electrical fundamentals and how they relate to each other in order to better troubleshoot electrical failures.

Faced with the spread of militarized police training facilities known as "cop cities," grassroots activists are taking the call to #DefundPolice to cities nationwide.

In Tijuana, Uganda, and Gaza, refugees facing dispossession, displacement, and constant violence are finding moments of solace in the art of dance.

Tricia Hersey’s new book insists we have the power to refuse capitalism’s grind culture and instead prioritize rest. 

Mesoporous silicon nanoparticles co-deliver chemotherapeutic and genetic drugs with a time lag for more effectively countering cancer-cell protection mechanisms against drugs, as well as inhibiting cell growth and metastasis.

PABCO Roofing’s entire Signature Cut line, including Paramount, Cascade, and Paramount Advantage, has earned UL 2218 Class 4 impact resistance, offering enhanced durability and aesthetic appeal.

Many factors go into determining a roof's wind resistance, from speed and direction to the design and materials used. Westlake Royal Roofing Products takes a closer look at the way concrete roof tiles are a superior choice for wind resistance.

In the light of current world events, defiance will be in sharp relief at the 48th Göteborg Film Festival, which runs Jan. 26 – Feb. 4, 2025 Under the banner “Focus: Disobedience,” the festival will highlight themes of civil resistance and pose such questions as: “When does rebellion become a moral obligation? When can it […]

The Sermon on the Mount is one of the greatest gifts of scripture to humanity; just ask Mahatma Gandhi, Martin Luther King Jr., and Leo Tolstoy. In a time when an eye for an eye still seems to hold sway, IDEAS producer Sean Foley explores the logic of Christian non-violence, beginning with Jesus' counsel to 'turn the other cheek.'

An important mechanism of resistance to β-lactam antibiotics is via their β-lactamase–catalyzed hydrolysis. Recent work has shown that, in addition to the established hydrolysis products, the reaction of the class D nucleophilic serine β-lactamases (SBLs) with carbapenems also produces β-lactones. We report studies on the factors determining β-lactone formation by class D SBLs. We show that variations in hydrophobic residues at the active site of class D SBLs (i.e. Trp105, Val120, and Leu158, using OXA-48 numbering) impact on the relative levels of β-lactones and hydrolysis products formed. Some variants, i.e. the OXA-48 V120L and OXA-23 V128L variants, catalyze increased β-lactone formation compared with the WT enzymes. The results of kinetic and product studies reveal that variations of residues other than those directly involved in catalysis, including those arising from clinically observed mutations, can alter the reaction outcome of class D SBL catalysis. NMR studies show that some class D SBL variants catalyze formation of β-lactones from all clinically relevant carbapenems regardless of the presence or absence of a 1β-methyl substituent. Analysis of reported crystal structures for carbapenem-derived acyl-enzyme complexes reveals preferred conformations for hydrolysis and β-lactone formation. The observation of increased β-lactone formation by class D SBL variants, including the clinically observed carbapenemase OXA-48 V120L, supports the proposal that class D SBL-catalyzed rearrangement of β-lactams to β-lactones is important as a resistance mechanism.

Erythromycin-resistance methyltransferases are SAM dependent Rossmann fold methyltransferases that convert A2058 of 23S rRNA to m6 2A2058. This modification sterically blocks binding of several classes of antibiotics to 23S rRNA, resulting in a multidrug-resistant phenotype in bacteria expressing the enzyme. ErmC is an erythromycin resistance methyltransferase found in many Gram-positive pathogens, whereas ErmE is found in the soil bacterium that biosynthesizes erythromycin. Whether ErmC and ErmE, which possess only 24% sequence identity, use similar structural elements for rRNA substrate recognition and positioning is not known. To investigate this question, we used structural data from related proteins to guide site-saturation mutagenesis of key residues and characterized selected variants by antibiotic susceptibility testing, single turnover kinetics, and RNA affinity–binding assays. We demonstrate that residues in α4, α5, and the α5-α6 linker are essential for methyltransferase function, including an aromatic residue on α4 that likely forms stacking interactions with the substrate adenosine and basic residues in α5 and the α5-α6 linker that likely mediate conformational rearrangements in the protein and cognate rRNA upon interaction. The functional studies led us to a new structural model for the ErmC or ErmE-rRNA complex.

Investigations of bacterial resistance strategies can aid in the development of new antimicrobial drugs as a countermeasure to the increasing worldwide prevalence of bacterial antibiotic resistance. One such strategy involves the TipA class of transcription factors, which constitute minimal autoregulated multidrug resistance (MDR) systems against diverse antibiotics. However, we have insufficient information regarding how antibiotic binding induces transcriptional activation to design molecules that could interfere with this process. To learn more, we determined the crystal structure of SkgA from Caulobacter crescentus as a representative TipA protein. We identified an unexpected spatial orientation and location of the antibiotic-binding TipAS effector domain in the apo state. We observed that the α6–α7 region of the TipAS domain, which is canonically responsible for forming the lid of antibiotic-binding cleft to tightly enclose the bound antibiotic, is involved in the dimeric interface and stabilized via interaction with the DNA-binding domain in the apo state. Further structural and biochemical analyses demonstrated that the unliganded TipAS domain sterically hinders promoter DNA binding but undergoes a remarkable conformational shift upon antibiotic binding to release this autoinhibition via a switch of its α6–α7 region. Hence, the promoters for MDR genes including tipA and RNA polymerases become available for transcription, enabling efficient antibiotic resistance. These insights into the molecular mechanism of activation of TipA proteins advance our understanding of TipA proteins, as well as bacterial MDR systems, and may provide important clues to block bacterial resistance.

The development of a chronic, low-grade inflammation originating from adipose tissue in obese subjects is widely recognized to induce insulin resistance, leading to the development of type 2 diabetes. The adipose tissue microenvironment drives specific metabolic reprogramming of adipose tissue macrophages, contributing to the induction of tissue inflammation. Uncoupling protein 2 (UCP2), a mitochondrial anion carrier, is thought to separately modulate inflammatory and metabolic processes in macrophages and is up-regulated in macrophages in the context of obesity and diabetes. Here, we investigate the role of UCP2 in macrophage activation in the context of obesity-induced adipose tissue inflammation and insulin resistance. Using a myeloid-specific knockout of UCP2 (Ucp2ΔLysM), we found that UCP2 deficiency significantly increases glycolysis and oxidative respiration, both unstimulated and after inflammatory conditions. Strikingly, fatty acid loading abolished the metabolic differences between Ucp2ΔLysM macrophages and their floxed controls. Furthermore, Ucp2ΔLysM macrophages show attenuated pro-inflammatory responses toward Toll-like receptor-2 and -4 stimulation. To test the relevance of macrophage-specific Ucp2 deletion in vivo, Ucp2ΔLysM and Ucp2fl/fl mice were rendered obese and insulin resistant through high-fat feeding. Although no differences in adipose tissue inflammation or insulin resistance was found between the two genotypes, adipose tissue macrophages isolated from diet-induced obese Ucp2ΔLysM mice showed decreased TNFα secretion after ex vivo lipopolysaccharide stimulation compared with their Ucp2fl/fl littermates. Together, these results demonstrate that although UCP2 regulates both metabolism and the inflammatory response of macrophages, its activity is not crucial in shaping macrophage activation in the adipose tissue during obesity-induced insulin resistance.