Petrol sales were down 60.43 per cent to 9,73,000 tonnes in April

The synthesis and structural characterization of the mol­ecular compound (μ3-benzene-1,2-di­thiol­ato)hexa­carbonyl­bis­(μ3-1,1,1,4,4,4-hexafluorobut-2-ene-2,3-dithiolato)tricobaltmolybdenum, [Co3Mo(C4F6S2)2(C6H4S2)(CO)6] or Mo(tfd)2(bdt)(Co(CO)2)3 (tfd is 1,1,1,4,4,4-hexafluorobut-2-ene-2,3-dithiolate and bdt is benzene-1,2-di­thiol­ate), are reported. The structure of the mol­ecule contains the molybdenum tris­(di­thiol­ene) complex Mo(tfd)2(bdt) coordinated as a multidentate ligand to three cobalt dicarbonyl units. Each of the three cobalt centers is relatively close to molybdenum, with Co⋯Mo distances of 2.7224 (7), 2.8058 (7), and 2.6320 (6) Å. Additionally, each of the cobalt centers is bound via main-group donor atoms, but each one in a different way: the first cobalt atom is coordinated by two sulfur atoms from different di­thiol­enes (bdt and tfd). The second cobalt atom is coordinated by one sulfur from one tfd and two olefinic carbons from another tfd. The third cobalt is coordinated by one sulfur from bdt and two sulfurs from tfd. This is, to the best of our knowledge, the first structurally characterized example of a molybdenum (tris­)di­thiol­ene complex that coordinates to cobalt. The F atoms of two of the –CF3 groups were refined as disordered over two sets of sites with ratios of refined occupancies of 0.703 (7):0.297 (7) and 0.72 (2):0.28 (2).

Transforming growth factor β-1 (TGFβ-1) is a secreted signalling protein that directs many cellular processes and is an attractive target for the treatment of several diseases. The primary endogenous activity regulatory mechanism for TGFβ-1 is sequestration by its pro-peptide, latency-associated peptide (LAP), which sterically prohibits receptor binding by caging TGFβ-1. As such, recombinant LAP is promising as a protein-based therapeutic for modulating TGFβ-1 activity; however, the mechanism of binding is incompletely understood. Comparison of the crystal structure of unbound LAP (solved here to 3.5 Å resolution) with that of the bound complex shows that LAP is in a more open and extended conformation when unbound to TGFβ-1. Analysis suggests a mechanism of binding TGFβ-1 through a large-scale conformational change that includes contraction of the inter-monomer interface and caging by the `straight-jacket' domain that may occur in partnership through a loop-to-helix transition in the core jelly-roll fold. This conformational change does not appear to include a repositioning of the integrin-binding motif as previously proposed. X-ray scattering-based modelling supports this mechanism and reveals possible orientations and ensembles in solution. Although native LAP is heavily glycosylated, solution scattering experiments show that the overall folding and flexibility of unbound LAP are not influenced by glycan modification. The combination of crystallography, solution scattering and biochemical experiments reported here provide insight into the mechanism of LAP sequestration of TGFβ-1 that is of fundamental importance for therapeutic development.

Copper-containing nitrite reductases (CuNiRs) are found in all three kingdoms of life and play a major role in the denitrification branch of the global nitro­gen cycle where nitrate is used in place of di­oxy­gen as an electron acceptor in respiratory energy metabolism. Several C- and N-terminal redox domain tethered CuNiRs have been identified and structurally characterized during the last decade. Our understanding of the role of tethered domains in these new classes of three-domain CuNiRs, where an extra cytochrome or cupredoxin domain is tethered to the catalytic two-domain CuNiRs, has remained limited. This is further compounded by a complete lack of substrate-bound structures for these tethered CuNiRs. There is still no substrate-bound structure for any of the as-isolated wild-type tethered enzymes. Here, structures of nitrite and product-bound states from a nitrite-soaked crystal of the N-terminal cupredoxin-tethered enzyme from the Hyphomicrobium denitrificans strain 1NES1 (Hd1NES1NiR) are provided. These, together with the as-isolated structure of the same species, provide clear evidence for the role of the N-terminal peptide bearing the conserved His27 in water-mediated anchoring of the substrate at the catalytic T2Cu site. Our data indicate a more complex role of tethering than the intuitive advantage for a partner-protein electron-transfer complex by narrowing the conformational search in such a combined system.

Bacterial cytokinesis is mediated by the Z-ring, which is formed by the prokaryotic tubulin homolog FtsZ. Recent data indicate that the Z-ring is composed of small patches of FtsZ protofilaments that travel around the bacterial cell by treadmilling. Treadmilling involves a switch from a relaxed (R) state, favored for monomers, to a tense (T) conformation, which is favored upon association into filaments. The R conformation has been observed in numerous monomeric FtsZ crystal structures and the T conformation in Staphylococcus aureus FtsZ crystallized as assembled filaments. However, while Escherichia coli has served as a main model system for the study of the Z-ring and the associated divisome, a structure has not yet been reported for E. coli FtsZ. To address this gap, structures were determined of the E. coli FtsZ mutant FtsZ(L178E) with GDP and GTP bound to 1.35 and 1.40 Å resolution, respectively. The E. coli FtsZ(L178E) structures both crystallized as straight filaments with subunits in the R conformation. These high-resolution structures can be employed to facilitate experimental cell-division studies and their interpretation in E. coli.

Shares of WPX Energy traded 25% higher after President Trump tweeted that he expected a deal between Russia and Saudi Arabia to scale back oil production.

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The management of international rivers is subject to two main types of boundary: the physical boundary of the river itself and the socio-political boundaries of the nations and regions that surround it. A new study has compared the impacts of managing rivers according to these different boundaries in Europe and Africa.

New research in Estonia indicates that plant biodiversity in field boundaries varies with features, such as ditches, trees and nearby roads. It suggests that, in addition to common non-weedy plants, more consideration should be given to rare weeds by broadening of field boundaries and reducing the use of agrochemicals and fertilisers near these boundaries.

Civilisation has crossed four of nine ‘planetary boundaries’, increasing the risk of irreversibly driving the Earth in to a less hospitable state, concludes new research. These are: extinction rate, deforestation, atmospheric CO2 and the flow of nitrogen and phosphorus.

A methodology for assessing ‘chemical footprints’ has been developed by researchers to evaluate human pressures and the impact of chemicals released by the production and consumption of goods. The study integrates a life-cycle approach with different methodologies, such as those developed in the context of environmental risk assessment and sustainability science, with the aim of assessing the extent to which chemicals impact on ecosystems beyond their ability to recover (i.e. surpass planetary boundaries).

In order to manage its environmental footprint, Switzerland should act on a number of key issues identified by the ‘planetary boundaries’ framework, says a Swiss study, with priority given to the areas of climate change, ocean acidification, biodiversity loss and nitrogen loss. This quantitative framework identifies nine bio-physical limits of the Earth system that, if exceeded, may lead to societal and ecological changes unfavourable to human development and stability. These are upper thresholds rather than targets. The researchers suggest that the concept and their methodology could be used together to think differently about environmental issues, and change the way related assessments and policies are implemented at both global and national levels.

A BT One Enterprise Cisco solution makes voice calls between the UK and India simple and immediate. In most cases they’re free of charge too. Best of all, the employees feel as one with the BT global team.

Transactions will fall by up to 60% this spring then steadily tick back up, with a rebound by the end of 2021, according to most likely scenario forecast by Zillow

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CULPEPER —  A new traffic pattern will take effect next week as construction begins on the westbound Route 211/522 (Lee Highway) bridge over the...

  FREDERICKSBURG, Va. – (7 a.m.) An early morning crash has closed all lanes on Route 1 southbound and one lane on Route 1 northbound at Austin...

SALEM – As part of the ongoing construction to replace the bridge over Back Creek on Route 220, an extended lane closure has been scheduled for next week. The bridge is near the intersection of Route 657 (Crowell Gap Road and Winter Drive).  Beginning Monday, October 8 at 9 a.m., the right northbound lane of Route 220 and the crossover at Crowell Gap Road will be closed until Wednesday, October 10 at 5 p.m. to pave a segment of the roadway that will connect to the new bridge. Drivers traveling on Route 220 northbound into Roanoke should expect significant delays on Route 220 during this time and are encouraged to plan their travel accordingly. The bridge replacement is expected to be complete in spring 2020. For updates on this project and other traffic impacts and lane closure, visit www.511Virginia.org .

SALEM – The left lane of southbound Route 220 is closed due to a slope failure in the median just south of the Franklin County line. The lane closure begins approximately one mile prior to  the Roanoke County and Franklin County line and just north of the intersection with Route 613 (Naff Road).   Drivers should prepare for travel delays and plan accordingly. Message boards are in place to alert motorists of the closure. The Virginia Department of Transportation is currently investigating to determine what repairs need to be made. The lane will remain closed until further notice.   For updates on this lane closure, visit www.511Virginia.org .

SALEM – The Virginia Department of Transportation encourages race fans driving to and from Martinsville Speedway for the race on Sunday, October 28, to follow recommended traffic patterns as posted on signs and message boards. Drivers travelling from Roanoke to the Martinsville Speedway for the race events this weekend should plan for possible delays due to a left lane closure on Route 220 southbound approaching the Boones Mill area. VDOT will place many signs and changeable message boards throughout the region to assist travelers. Vehicular and pedestrian traffic will be heavy. Traffic is being routed to the speedway in certain patterns to minimize overall congestion as much as possible. Watch for the speed limit to reduce gradually from 65 mph to 35 mph on the Route 220/Route 58 Bypass as you approach the speedway. From Roanoke and areas north of Martinsville: Race fans traveling south on Route 220 are encouraged to allow for extra travel time. A left lane closure is in place for a pipe failure in the median about eight miles south of Roanoke and 1.7 miles north of Boones Mill. Drivers can take the exit onto Route 58 to Martinsville to reach the track or they can continue on the Route 220/Route 58 Bypass past the speedway interchange and turn right onto the access road that leads into the speedway. The entrance is located one mile east of the Route 220 interchange and may provide easier access. Signs are in place designating the entrance to the speedway.  From Danville and areas east of Martinsville: Motorists on the Route 58 Bypass should follow signs directing them to use the track’s back gate. This entrance is located off the bypass, east of the track. From Greensboro and areas south of Martinsville: Route 220 northbound traffic will split into three lanes shortly before reaching the speedway.  Motorists should watch for electronic message boards and obey police directing traffic. Through traffic should use the left lane. From Stuart and areas west of Martinsville: Motorists should stay on Route 58 east and follow signs and message boards to the track.  ADA Parking: Race fans needing ADA Parking are encouraged to enter the Speedway via Old Sand Roa Those attending the race should closely follow directional information and plan to arrive early and expect heavy traffic and delays. Martinsville Speedway has shared the attached graphics for drivers entering and exiting from the race.

The on-ramp and off-ramp between northbound Interstate 81 and Route 262 (Woodrow Wilson Parkway) at exit 220 in Augusta County are scheduled for overnight closures and detours during the week of October 21-25, 2018. A northbound I-81 right-lane closure will also be in place during this time period. These traffic restrictions allow milling and paving of existing ramps, and an extension of the acceleration lane from Route 262 to northbound I-81.

This week motorists should expect delays in the vicinity of Interstate 64 exit 87 westbound at Interstate 81 exit 221 southbound in Augusta County. There will be bridge deck work on the southbound ramp bridge from I-64 westbound to I-81 southbound. The right lane will be closed.   

A Barcelona design duo work impressive DIY restoration magic on a long-abandoned comital residence-turned-storehouse-turned-apartment.

The organic produce company may sell nationally, but it still keeps a roadside stand in Central California where people can buy organic produce and much more.

The modern prefab specialists at Modscape went for the obvious when naming this doozy of a concept home. But hey, it fits.

Tree rings of old eastern red cedars show improved tree growth and physiology since the Clean Air Act's implementation in the 1980s.

Conservation efforts pay off for mountain gorillas, which have been re-classified from "critically endangered" to "endangered" by IUCN.

Almost 50 years after Aldrin ate oatmeal on Apollo 11, Quaker uses his breakfast of choice in an inspirational ad not aired in the U.S.

A tiny satellite built by grade schoolers may get a little help from above during its launch.

There's an 'urgent need' for rules governing the use of lab-grown brains.

The holiday season is a weighted one, full of food and food-pushers. Here's how to gently, but firmly, stand your ground.

Brief description about the advantage of OBT helping your company's team management.

Author Carlos Hidalgo discusses how he exited the fast lane for greater fulfillment, and shares insight from his new book, The UnAmerican Dream: Finding Personal and Professional Happiness Establishing Work-Life Boundaries.

REP Lab Director Jay Dicharry PT, will assist with FC Timber athlete development through specific strength, power and agility programs. Rebound Physical Therapy joins as "Official PT Provider to the Bend FC Timbers."

There are considerable opportunities in the overseas security market, especially the national market along the "The Belt and Road Initiative," which is an opportunity for China's security brands to make great achievements.

A location-tracking app to alert caregivers when a loved one wanders from a defined safe-zone—especially important during covid-19.

Physical Therapist designed exercise program

"Professor J" has been blazing trails in applied computational chemistry for pharmaceutical and cosmetic color additive research since her Rowan University days, and has actively passed on her love of the profession by teaching the next generation.

Remote employment service Boundless and Passbase, the identity verification provider, today announced that they have partnered up to deliver a seamless and secure employment onboarding experience.