Following the rebound seen over the course of the previous session, stocks are seeing further upside in morning trading on Tuesday. The major averages have all climbed firmly into positive territory, further offsetting the pullback seen late last week.

European stocks rebounded on Tuesday after falling sharply the previous day on concerns about the coronavirus's impact on earnings and rising U.S.-China tensions.

The South Korea stock market has finished lower in two of three trading days since the end of the three-day winning streak in which it had collected almost 60 points or 3.1 percent. The KOSPI remains just beneath the 1,930-point plateau although it may bounce higher again on Friday.

Ahead of Thursday's holiday for Vesak Day, the Malaysia stock market had finished lower in two of three trading days since the end of the four-day winning streak in which it had climbed almost 40 points or 3 percent. The Kuala Lumpur Composite Index now rests just above the 1,375-point plateau although it may see renewed support on Friday.

After spearheading the fight to combat the summer's devastating bushfires, NSW RFS Commissioner Shane Fitzsimmons leaves his job today and says his new role will take on the bushfire recovery efforts.

James Blake brought is soulful electric music to the Astor

Meet the Tasmanian circus performer who's determined to teach kids and adults how flying through the air and hanging upside down can reframe how we think about bodies, boundaries and consent.

Despite Labor's belief in a "mood for change" in Victoria, the only seats in the state likely to switch parties are those where boundaries had been redrawn since the last election.

A teenage basketball star from a celebrity family in California is set to move to Wollongong in New South Wales in one of the most intriguing signings in Australian basketball history.

With the imminent arrival of a new salmon processing ship in Tasmania, and another even larger ship next month, some in the local sailing community fear there is "an accident waiting to happen" on the already very busy waterways.

It will take a test case a Canberra smoker who police decide to arrest and charge to determine what the law actually is.

THE installation of a special boundary to help Orthodox Jewish families carry out normal activities on holy days has erupted into a fight with council.

Canadian Inuk Kelly Fraser Has Been Making Waves With Her Single “Rebound Girl” Gaining Commercial Radio Play Across Canada.

Kieran Cebrian began playing hockey at age 4 where it was most convenient. From the family home in Wash Park, he could walk to the University of Denver.

Now that Colorado is homebound for a while, here are three regional books to read during the coronavirus outbreak.

Hello Internet! In This Episode: Erin and Weer’d talk about a defensive gun use that stopped a potential mass shooting, then discuss the assorted nuttery happening in relationship to the COVID-19 quarantine; Loveball returns to the show to talk about what … Continue reading →

Outbound linking it seems to go against the grain to get your site noticed. You’ve probably heard over and over again about the importance of having high quality back links pointing to your site, these links help boost your search ranking, page rank, and hopefully get you some more traffic. Outbound linking you would think would be the opposite of that. It turns out though that outbound links are good for you. Having quality outbound links can have some big benefits to your website or blog...................

In an effort to find a balance between protecting the significant investment in our cellular devices and protecting the planet, LifeProof has developed a phone case that sources materials diverted from the ocean and simultaneously supports organizations directly involved in providing safe water, protecting ocean life and maintaining river habitat. [...]

“We’ve learned to assist each other, to help each other and listen,” said Magda Melendez, Citymeals warehouse distribution manager. “We leave aside the mother-daughter relationship and cooperate like best friends.”

“We’ve learned to assist each other, to help each other and listen,” said Magda Melendez, Citymeals warehouse distribution manager. “We leave aside the mother-daughter relationship and cooperate like best friends.”

“We’ve learned to assist each other, to help each other and listen,” said Magda Melendez, Citymeals warehouse distribution manager. “We leave aside the mother-daughter relationship and cooperate like best friends.”

During a pandemic that puts class divisions into sharper focus, this acclaimed "Ironbound" gets a benefit reading by its original New York cast.

The OECD (Organisation for Economic Co-operation and Development) recently published new preliminary figures for aid in 2013.

It showed official development assistance (ODA) aid from wealthy governments had increased to just under $135 billion in 2013 (at constant 2012 prices). This is roughly 0.3% of GNI (Gross National Income) of the donor nations.

Yet, over 40 years ago nations promised to reach 0.7% of their GNI by the mid-1970s. While each year the amount of aid falls quite short of that 0.7% target (less than half of that target), the quality and effectiveness of that aid is often questionable, sometimes benefiting the donor more than the recipient due to the types of conditions attached to this aid.

This update includes a number of new and updated charts and graphs.

Read full article: Foreign Aid for Development Assistance

Human leukocyte antigen (HLA) B*51:01 and endoplasmic reticulum aminopeptidase 1 (ERAP1) are strongly genetically associated with Behcet's disease (BD). Previous studies have defined two subgroups of HLA-B*51 peptidome containing proline (Pro) or alanine (Ala) at position 2 (P2). Little is known about the unconventional non-Pro/Ala2 HLA-B*51-bound peptides. We aimed to study the features of this novel subpeptidome, and investigate its regulation by ERAP1. CRISPR-Cas9 was used to generate an HLA-ABC-triple knockout HeLa cell line (HeLa.ABC-KO), which was subsequently transduced to express HLA-B*51:01 (HeLa.ABC-KO.B51). ERAP1 was silenced using lentiviral shRNA. Peptides bound to HLA-B*51:01 were eluted and analyzed by mass spectrometry. The characteristics of non-Pro/Ala2, Pro2, and Ala2 peptides and their alteration by ERAP1 silencing were investigated. Effects of ERAP1 silencing on cell surface expression of HLA-B*51:01 were studied using flow cytometry. More than 20% of peptides eluted from HLA-B*51:01 lacked Pro or Ala at P2. This unconventional group of HLA-B*51:01-bound peptides was relatively enriched for 8-mers (with relatively fewer 9-mers) compared with the Pro2 and Ala2 subpeptidomes and had similar N-terminal and C-terminal residue usages to Ala2 peptides (with the exception of the less abundant leucine at position ). Knockdown of ERAP1 increased the percentage of non-Pro/Ala2 from 20% to ~40%, increased the percentage of longer (10-mer and 11-mer) peptides eluted from HLA-B*51:01 complexes, and abrogated the predominance of leucine at P1. Interestingly knockdown of ERAP1 altered the length and N-terminal residue usage of non-Ala2&Pro2 and Ala2 but not the Pro2 peptides. Finally, ERAP1 silencing regulated the expression levels of cell surface HLA-B*51 in a cell-type-dependent manner. In conclusion, we have used a novel methodology to identify an unconventional but surprisingly abundant non-Pro/Ala2 HLA-B*51:01 subpeptidome. It is increased by knockdown of ERAP1, a gene affecting the risk of developing BD. This has implications for theories of disease pathogenesis.

V. A. Sadovnichii, Ya. T. Sultanaev and A. M. Akhtyamov
Trans. Moscow Math. Soc. 80 (2020), 123-131.
Abstract, references and article information

S. A. Nazarov
Trans. Moscow Math. Soc. 80 (2020), 1-51.
Abstract, references and article information

Yuji Nakatsukasa
Math. Comp. 89 (2020), 1843-1866.
Abstract, references and article information

Albert Chau and Man-Chun Lee
Trans. Amer. Math. Soc. 373 (2020), 3627-3647.
Abstract, references and article information

Cătălin I. Cârstea, Gen Nakamura and Lauri Oksanen
Trans. Amer. Math. Soc. 373 (2020), 3423-3443.
Abstract, references and article information

Guher Camliyurt, Igor Kukavica and Vlad Vicol
Trans. Amer. Math. Soc. 373 (2020), 3375-3422.
Abstract, references and article information

Daniel Carando, Santiago Muro and Daniela M. Vieira
Proc. Amer. Math. Soc. 148 (2020), 2447-2457.
Abstract, references and article information

Claude Cibils, Marcelo Lanzilotta, Eduardo N. Marcos and Andrea Solotar
Proc. Amer. Math. Soc. 148 (2020), 2421-2432.
Abstract, references and article information

Liye Chen
May 1, 2020; 19:871-883
Research

Human leukocyte antigen (HLA) B*51:01 and endoplasmic reticulum aminopeptidase 1 (ERAP1) are strongly genetically associated with Behcet's disease (BD). Previous studies have defined two subgroups of HLA-B*51 peptidome containing proline (Pro) or alanine (Ala) at position 2 (P2). Little is known about the unconventional non-Pro/Ala2 HLA-B*51-bound peptides. We aimed to study the features of this novel subpeptidome, and investigate its regulation by ERAP1. CRISPR-Cas9 was used to generate an HLA-ABC-triple knockout HeLa cell line (HeLa.ABC-KO), which was subsequently transduced to express HLA-B*51:01 (HeLa.ABC-KO.B51). ERAP1 was silenced using lentiviral shRNA. Peptides bound to HLA-B*51:01 were eluted and analyzed by mass spectrometry. The characteristics of non-Pro/Ala2, Pro2, and Ala2 peptides and their alteration by ERAP1 silencing were investigated. Effects of ERAP1 silencing on cell surface expression of HLA-B*51:01 were studied using flow cytometry. More than 20% of peptides eluted from HLA-B*51:01 lacked Pro or Ala at P2. This unconventional group of HLA-B*51:01-bound peptides was relatively enriched for 8-mers (with relatively fewer 9-mers) compared with the Pro2 and Ala2 subpeptidomes and had similar N-terminal and C-terminal residue usages to Ala2 peptides (with the exception of the less abundant leucine at position ). Knockdown of ERAP1 increased the percentage of non-Pro/Ala2 from 20% to ~40%, increased the percentage of longer (10-mer and 11-mer) peptides eluted from HLA-B*51:01 complexes, and abrogated the predominance of leucine at P1. Interestingly knockdown of ERAP1 altered the length and N-terminal residue usage of non-Ala2&Pro2 and Ala2 but not the Pro2 peptides. Finally, ERAP1 silencing regulated the expression levels of cell surface HLA-B*51 in a cell-type-dependent manner. In conclusion, we have used a novel methodology to identify an unconventional but surprisingly abundant non-Pro/Ala2 HLA-B*51:01 subpeptidome. It is increased by knockdown of ERAP1, a gene affecting the risk of developing BD. This has implications for theories of disease pathogenesis.

The presentation of post-translationally modified (PTM) peptides by cell surface HLA molecules has the potential to increase the diversity of targets for surveilling T cells. Whilst immunopeptidomics studies routinely identify thousands of HLA-bound peptides from cell lines and tissue samples, in-depth analyses of the proportion and nature of peptides bearing one or more PTMs remains challenging. Here we have analyzed HLA-bound peptides from a variety of allotypes and assessed the distribution of mass spectrometry-detected PTMs, finding deamidation of asparagine or glutamine to be highly prevalent. Given that asparagine deamidation may arise either spontaneously or through enzymatic reaction, we assessed allele-specific and global motifs flanking the modified residues. Notably, we found that the N-linked glycosylation motif NX(S/T) was highly abundant across asparagine-deamidated HLA-bound peptides. This finding, demonstrated previously for a handful of deamidated T cell epitopes, implicates a more global role for the retrograde transport of nascently N-glycosylated polypeptides from the ER and their subsequent degradation within the cytosol to form HLA-ligand precursors. Chemical inhibition of Peptide:N-Glycanase (PNGase), the endoglycosidase responsible for the removal of glycans from misfolded and retrotranslocated glycoproteins, greatly reduced presentation of this subset of deamidated HLA-bound peptides. Importantly, there was no impact of PNGase inhibition on peptides not containing a consensus NX(S/T) motif. This indicates that a large proportion of HLA-I bound asparagine deamidated peptides are generated from formerly glycosylated proteins that have undergone deglycosylation via the ER-associated protein degradation (ERAD) pathway. The information herein will help train deamidation prediction models for HLA-peptide repertoires and aid in the design of novel T cell therapeutic targets derived from glycoprotein antigens.

Kevin Kiermaier has always played with a chip on his shoulder, but he admits that there's some extra motivation heading into Spring Training after a frustrating 2018 season.

And I’ll be dust to dust bound to, Shifting black wicker furniture just so, Patrons aren’t disturbed, By the rain between, The hair-do and the pedicure, separated At least, By three floors if the lifts are working, me I’m just Splashing mops against mauve tile, they Flashing manicured smiles, we Electing marvelous politicians in fashionable leather, […]

The U.S. government is operating accelerated dockets to handle the rising number of cases of families in immigration court. While it is essential to have timely, fair case processing and removal of those who have truly had their day in court and been found to be removable, using “rocket” dockets to speed up proceedings only heightens the breakdowns that are a recurring feature of the court system on its best day, as this commentary explains.

Registration opens April 22 for the ADA FDC Annual Meeting, which will offer a variety of learning and social opportunities for dentists and their teams to enjoy.

Clementi, Tyler.

All-Pac-12 talent Mikayla Pivec's career in Corvallis has been memorable to say the least. While it's difficult to choose just three, her top moments include a career-high 19 rebounds against Washington, a buzzer-beating layup against ASU, and breaking Ruth Hamblin's Oregon State rebounding record this year against Stanford.

Ruthy Hebard, who ranks 2nd in Oregon history in points (2,368) and 3rd in rebounds (1,299), prepares to play in the WNBA following four years in Eugene. Hebard is the Oregon and Pac-12 all-time leader in career field-goal percentage (65.1) and averaged 17.3 points per game and a career-high 9.6 rebounds per game as a senior.