An Albanian man's heart is forever changed after suffering a heart attack and meeting Jesus in his recuperation.

Doron's experience on Logos Hope shows him God's faithfulness and uncovers leadership abilities he is using today in a new role.

An OM worker in Cambodia shares about how a new training she is attending is transforming the way she does ministry.

Boston Bruins goaltender Jaroslav Halak signed a one-year contract worth $2.25 million US, the team announced Friday. He was playing in the final season of a two-year contract.

The NHL is hoping to convince teams the league should move the 2020 draft to early June and hold it virtually, according to a report.

Veteran forward Kelly Babstock signed with the new Toronto franchise of the National Women's Hockey League on Saturday. The two-time all-star has 27 goals and 60 points in 65 games over four seasons.

A normal draft year would see NHL scouts and executives spend the spring crisscrossing Europe and North America collecting information. The circumstances surrounding a 2020 draft and the COVID-19 pandemic, however, aren't anything resembling normal.

Former Montreal Canadiens enforcer Georges Laraque's biggest challenge is trying to breathe clearly as he fights COVID-19 at a Montreal hospital. "The nights are the worst. I have fevers a couple times a night. I have to get up and take pills."

Prime Minister Justin Trudeau said Sunday that NHL players would — at a minimum — need to follow quarantine protocols if they were to arrive in Canada while the border remains closed due to the pandemic.

The Toronto Maple Leafs have signed Finnish defenceman Mikko Lehtonen to a one-year, entry-level contract for the 2020-21 season, the club announced Monday.

Pittsburgh Penguins superstar Sidney Crosby is easing the burden for many Pennsylvania families struggling during the COVID-19 virus.

The coronavirus pandemic has forced major professional sports leagues across North America to push the pause button. But even if the NHL, CFL, NBA or MLS are given the green light, many fans have reservations about returning to arenas until a vaccine for the virus is available.

Ontario Premier Doug Ford says the Toronto Maple Leafs' parent company has been in contact with the province about the possibility of Canada's biggest city serving as a so-called "hockey pod" for teams should the NHL resume its season.

Just when you thought you watched the best shot of the Brier, another best shot of the Brier appears at the 2020 Canadian men's curling championship being held in Kingston, Ont.

Alberta's Brendan Bottcher defeated Saskatchewan's Matt Dunstone 9-4 in the 1-2 Page playoff game Saturday afternoon to earn a berth in the Tim Hortons Brier final.

Team Homan, Canada’s 2018 Olympic women’s curling team and the 2017 World Women’s Curling Champions have announced that the team has parted ways with lead Lisa Weagle. 

Team Homan's decision to part ways with Lisa Weagle dramatically changed the look of one of the country's top rinks and put the all-star lead at the top of curling's free agent list.

The University of Alberta Pandas defeated the UNB Reds 10-2 in the U Sports women's curling final, while the Laurier Golden Hawks topped the Dalhousie Tigers 8-5 for the men's curling title in Portage la Prairie, Manitoba on Sunday.

The Kevin Koe curling team has split with second Colton Flasch after being eliminated from the recent men's national championship in Kingston, Ont., in a tiebreaker game. Flasch joined the foursome in 2018.

In the span of a few days, Canada's curling landscape has shifted dramatically. The country's past women's and men's Olympic teams and last year's women's world champion team have all split.

John Morris will play second for the Kevin Koe's team for at least the next 18 months heading into the Olympic trials in 2021.

Let go last week after a decade-long run with skip Rachel Homan's Ottawa-based rink, lead Lisa Weagle has joined Team Jennifer Jones, which will operate with five members.

The bonspiel took place in Edmonton March 11-14, starting the same day COVID-19 was declared a global pandemic.

The World Curling Federation announced Thursday it is proposing to the International Olympic Committee that the 2021 world curling championships will determine which countries will book their tickets to Beijing 2022.

Colleen Jones, Sidney Crosby among many preeminent local sports figures reacting in the aftermath of Sunday's killing rampage.

The time was right for Lisa Weagle to join forces with Olympic champion Jennifer Jones. Jones told CBC Sports' Anastasia Bucsis that the latest addition to her five-person rink "was like the clouds parted and the sun came out."

He’s a nominee in perpetual waiting.

He’s not rising to the challenge of the coronavirus pandemic. He’s shriveling into nothingness.

They’re pandemic partners, and that’s double trouble.

The notion that he is bound for four more years is pure superstition.

Laurie Garrett, the prophet of this pandemic, expects years of death and “collective rage.”

Fears that COVID-19 would spread at the Hamilton-Wentworth Detention Centre appear not to have materialized — at least not yet.

Curbside pickups offer a bit of hope after months of being shuttered by COVID-19, but while retailers are happy to start getting back to business some are raising questions of fairness and access to opportunity.

Flurries or rain showers are in the forecast for Hamilton on Friday, Environment Canada says.

Sofina Foods plants in Burlington and Mississauga have each had an employee test positive.

A search warrant executed by Hamilton police vice and drugs officers at an address in the city’s east end has resulted in the arrest of a couple and multiple charges, police said in a Friday release.

Dr. Roselyn Wilson, a psychiatrist at St. Joseph’s Healthcare Hamilton’s Youth Wellness Centre, offers some tips to help young people cope with the stress and anxiety they face while on lockdown under COVID-19.

Normal life is slowly resuming as the city of Hamilton begins to relax tight measures set in place because of COVID-19. Mayor Fred Eisenberger said now it's up to the public to help the city move in the right direction by continuing to stay two metres apart from each other and be cautious.

McMaster University is planning for fewer international students, more deferred acceptance offers and online teaching for the thousands who are attending as the start of a new school year looms in the near future and in the middle of the COVID-19 pandemic.

The general field of quantitative biology has advanced significantly on the back of recent improvements in both sequencing technology and proteomics methods. The development of high-throughput, short-read sequencing has revolutionized RNA-based expression studies, while improvements in proteomics methods have enabled quantitative studies to attain better resolution. Here we introduce methods to undertake global analyses of gene expression through RNA and protein quantification in Xenopus embryos and tissues.

Quantifying RNA is an important and necessary step before most RNA analysis techniques. Methods for quantifying RNA can be classified into two categories: ultraviolet (UV) spectrophotometric methods, which are based on the absorption spectra of the purine and pyrimidine bases; and fluorescent dye-based methods, which measure the fluorescence intensity of dyes that selectively fluoresce when bound to nucleic acids. If the RNA sample is pure (i.e., without significant amounts of contaminants such as proteins, phenol, agarose, or other nucleic acids), UV spectrophotometric measurement of the amount of UV irradiation absorbed by the bases is simple and accurate. However, if the sample contains significant quantities of impurities or if the concentration of RNA is very low, it is better to use fluorescent dye-based methods. An overview of spectrophotometric and fluorescent dye-based RNA quantification methods is given here, as are several options for storing purified RNA preparations. Proper storage of RNA samples is important; it can help minimize RNase contamination and consequent sample degradation.

Determining the concentration of protein samples generally is accomplished either by measuring the UV absorbance at 280 nm or by reacting the protein quantitatively with dyes and/or metal ions (Bradford, Lowry, or BCA assays). For purified proteins, UV absorbance remains the most popular method because it is fast, convenient, and reproducible; it does not consume the protein; and it requires no additional reagents, standards, or incubations. No method of protein concentration determination is perfect because each is subject to a different set of constraints such as interference of buffer components and contaminating proteins in direct UV determination (A₂₈₀) or reactivity of individual proteins and buffer components with the detecting reagents in colorimetric assays. In cases in which protein concentration is critical (e.g., determination of catalytic rate constants for an enzyme), it may be advisable to compare the results of several assays.

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