Proteomics Analysis of Interactions between Drug-Resistant and Drug-Sensitive Cancer Cells: Comparative Studies of Monoculture and Coculture Cell Systems  ACS Publications

Monopoly is one of the best-selling board games in history.

The game's staying power may in part be because of strong American lore — the idea that anyone, with just a little bit of cash, can rise from rags to riches. Mary Pilon, author of The Monopolists: Obsession, Fury, and the Scandal Behind the World's Favorite Board Game.
But there's another origin story – a very different one that promotes a very different image of capitalism. (And with two sets of starkly different rules.) That story shows how a critique of capitalism grew from a seed of an idea in a rebellious young woman's mind into a game legendary for its celebration of wealth at all costs.

This episode was made in collaboration with NPR's Throughline. For more about the origin story of Monopoly, listen to their original episode Do Not Pass Go.

This episode was produced by Emma Peaslee, mastered by Natasha Branch, and edited by Jess Jiang.

The Throughline episode was produced by Rund Abdelfatah, Ramtin Arablouei, Lawrence Wu, Laine Kaplan-Levenson, Julie Caine, Victor Yvellez, Anya Steinberg, Yolanda Sangweni, Casey Miner, Cristina Kim, Devin Katayama, and Amiri Tulloch. It was fact-checked by Kevin Volkl and mixed by Josh Newell.

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In 2022, artist Stuart Semple opened up his laptop to find that all his designs had turned black overnight. All the colors, across files on Adobe products like Photoshop and Illustrator, were gone. Who had taken the colors away? The story of what happened begins with one company, Pantone.

Pantone is known for their Color of the Year forecasts, but they actually make the bulk of their money from selling color reference guides. These guides are the standard for how designers pretty much anywhere talk about color.

On today's show, how did Pantone come to control the language of the rainbow? We look back at the history of Pantone, beginning with the man who made Pantone into the industry standard. And, we hear from Stuart, who tried to break the color monopoly.

Share your thoughts — What color should we choose to be Planet Money's color?

This episode was hosted by Sam Yellowhorse Kesler and Jeff Guo, and produced by Willa Rubin with help from James Sneed. It was edited by Jess Jiang and fact-checked by Sierra Juarez. Engineering by Debbie Daughtry with help from Carl Craft. Alex Goldmark is Planet Money's executive producer.

Help support Planet Money and hear our bonus episodes by subscribing to Planet Money+ in Apple Podcasts or at plus.npr.org/planetmoney.

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Selecting the correct LED lighting for machine vision can be challenging, even for experienced professionals. While red LEDs are commonly used, blue LEDs can be a superior choice for certain applications.

According to the Centers for Disease Control (CDC), accidental CO poisoning is the cause of approximately 50,000 emergency room visits and more than 400 deaths each year in the U.S.

In the first part of this episode we discuss a couple of basics about SecondLife (scaling, partitioning, etc). The second part specifically looks at how the dev team tackled a number of interesting problems in the context of executing their own LSL scripting language on top of Mono.

David Heinemeier Hansson, creator of the Ruby on Rails framework and a partner at the software development company Basecamp, talks to Stefan Tilkov about the state of Ruby on Rails and its suitability for long-term development. He addresses some of its common criticisms, such as perceived usefulness for only simple problems, claimed lack of scalability, and increasing complexity. David also talks about the downsides of building JavaScript-centric, “sophisticated” web UIs, and why he prefers well-structured, “majestic” monoliths to microservices.

Vaughn Vernon, author of the book “Strategic Monoliths and Microservices” discusses his book with host Akshay Manchale about strategies for purposeful architecture from the perspective of both business decision makers and technical leaders.

The FTC thinks Amazon hurts small businesses and consumers. Ron Knox of The Institute for Local Self-Reliance agrees.

A new DOJ lawsuit is challenging Live Nation and Ticketmaster’s monopoly power over artist performances, venues, and concert-goers.

The Rocky® MonoCrepe Wedge Work boots combine innovative design and advanced materials to deliver exceptional comfort and protection. Featuring a lightweight, oil- and slip-resistant outsole and a memory foam footbed, these waterproof boots ensure all-day support.

The latest advances in high-density polyethylene (HDPE) resin and production technology are making it possible to design all-PE structures for even the most demanding applications.

Amcor’s new AmSky blister packaging uses HDPE throughout for curbside recycling.

In addition to offering their entire fleet of available blister packaging systems ready to run these challenging films, the company will offer upgrades for installed Pharmaworks blister machines in the field and can rebuild blister machines from other suppliers to handle these new sustainable films.

Now available in downsized diameters of 13.5-16mm, the tiny tubes expand Neopac’s award-winning line of high-barrier mono-material solutions, and amount to a significant advancement in sustainability-minded high-barrier packaging technology.

All of Ultra Solo’s components – cap, pump engine, actuator and bottle – are polyethylene (PE). As such, the whole product boasts 100% recyclability.

Mondi’s mono-material solution provides high barriers against oxygen and water vapors, keeping the product fresh on the supermarket shelf.

The new tray from Waddington Europe does not require a polyethylene layer or adhesive coating.

Unexpected increase in consumer demand

Manufacturing

Manufacturing

Manufacturing

Lawmakers will take up an amendment requiring the Florida Department of Transportation to inspect Disney World's monorails.

Disney World’s monorail system is poised to get state oversight as Gov. Ron DeSantis battles the theme park giant.

Business technology solutions provider Brother UK has launched a compact range of mono laser printers for businesses, helping resellers to support customers with downsized offices and decentralised workforces.

Google called the DOJ extending search remedies to AI "radical," an "overreach."

If you keep on running out of dice, we have just the solution! Find all the latest Monopoly Go free dice links right here!

Current active events are Agent Bootcamp Event, Foam for Funds Event and Partner Event - Amazing Partners. The New Marvel Monopoly Go season has started. .

In a major blow to Google, a federal judge on Monday ruled that the tech giant maintained and abused a monopoly on web searches.

Apple has recently activated a feature that enables the HomePod and HomePod mini smart speakers to recognize the sound of smoke and carbon monoxide alarms and send a notification alert to your iPhone or iPad. In this episode of our podcast, Thomas Domville walks us through the process of setting this up.

The Sound Recognition feature uses the always-on microphone inside the HomePod to listen out for a home's existing smoke and carbon monoxide detector. When it hears the alarm, the HomePod sends a notification to your iPhone or iPad via the Home app. This means you don't need to invest in new internet-connected smoke alarms systems, as the HomePod can simply enhance the utility of your existing "dumb" offline smoke alarms. If you have HomeKit-compatible security cameras in your house, the notification will include a live video feed so you can remotely assess what's happening.

To enable Sound Recognition, follow these simple steps: open the Home app on your iPhone or iPad, then navigate to the Home Settings -> Safety & Security screen. There, you can enable the Sound Recognition feature. In addition, you can manage critical notification alerts from supported safety and security devices in your home, and even allow home members to "check in" and listen to the sounds in the home. It's worth noting that the Sound Recognition settings can also be accessed via a HomePod's dedicated Settings. Simply perform a long press on the HomePod's tile in the Home app to access its settings.

(If the Safety & Security section is dimmed out, ensure your HomePod and all other Apple devices are up-to-date with the latest software.

Neal Martin Williams has a new photo exhibition opening in Birmingham, says our arts staff

Escuche el programa de este jueves 7 de diciembre. La Luciérnaga, un espacio un espacio de humor y opinión de Caracol Radio que acompaña desde hace más de 30 años a sus oyentes en el regreso a casa.

Escuche el programa de este miércoles 10 de enero. La Luciérnaga, un espacio de humor y opinión de Caracol Radio que acompaña por más de 30 años a sus oyentes en su regreso a casa.

Escuche el programa de este jueves 11 de enero. La Luciérnaga, un espacio de humor y opinión de Caracol Radio que acompaña por más de 30 años a sus oyentes en su regreso a casa.

El gobierno del alcalde Jaume Collboni está trazando un muy complicado plan para blindar el distrito de Ciutat Vella contra los monocultivos comerciales. via Pocket

The real threat is collusion. When journalists strike secret alliances with the very people they're supposed to be holding accountable, we are in deep trouble. Lies go unchallenged.  Democracy cannot function. And that's what we're watching right now. Continue reading →

Embed from Getty Images In November of 2016, president elect Trump selected Betsy DeVos as his Secretary of Education. While this appointment seems to have changed her mind about Common Core, DeVos has remained committed to expanding charter schools. Charter …Read more »

Jorge H. Capdevila
Feb 1, 2000; 41:163-181
Reviews

Gurmeet K. Bakshi, Jyoti Garg and Gabriela Olteanu
Math. Comp. 93 (), 3027-3058.
Abstract, references and article information

Torin Greenwood, Jonathan Kariv and Noah Williams
Math. Comp. 93 (), 2959-2983.
Abstract, references and article information

Madison T Wright
Nov 18, 2020; 0:RA120.002168v1-mcp.RA120.002168
Research

Lipoprotein (a) [Lp(a)] is a risk factor for CVD and a target of therapy, but Lp(a) measurements are not globally standardized. Commercially available assays generally use polyclonal antibodies that detect multiple sites within the kringle (K)IV₂ repeat region of Lp(a) and may lead to inaccurate assessments of plasma levels. With increasing awareness of Lp(a) as a cardiovascular risk factor and the active clinical development of new potential therapeutic approaches, the broad availability of reagents capable of providing isoform independence of Lp(a) measurements is paramount. To address this issue, we generated a murine monoclonal antibody that binds to only one site on apo(a). A BALB/C mouse was immunized with a truncated version of apo(a) that contained eight total KIV repeats, including only one copy of KIV₂. We generated hybridomas, screened them, and successfully produced a KIV₂-independent monoclonal antibody, named LPA-KIV9. Using a variety of truncated apo(a) constructs to map its binding site, we found that LPA-KIV9 binds to KIV₉ without binding to plasminogen. Fine peptide mapping revealed that LPA-KIV9 bound to the sequence ⁴⁰⁷⁶LETPTVV⁴⁰⁸² on KIV₉. In conclusion, the generation of monoclonal antibody LPA-KIV9 may be a useful reagent in basic research studies and in the clinical application of Lp(a) measurements.

For three decades, the LPL–specific monoclonal antibody 5D2 has been used to investigate LPL structure/function and intravascular lipolysis. 5D2 has been used to measure LPL levels, block the triglyceride hydrolase activity of LPL, and prevent the propensity of concentrated LPL preparations to form homodimers. Two early studies on the location of the 5D2 epitope reached conflicting conclusions, but the more convincing report suggested that 5D2 binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The same loop had been implicated in lipoprotein binding. Using surface plasmon resonance, we showed that 5D2 binds with high affinity to a synthetic LPL peptide containing the Trp-rich loop of human (but not mouse) LPL. We also showed, by both fluorescence and UV resonance Raman spectroscopy, that the Trp-rich loop binds lipids. Finally, we used X-ray crystallography to solve the structure of the Trp-rich peptide bound to a 5D2 Fab fragment. The Trp-rich peptide contains a short α-helix, with two Trps projecting into the antigen recognition site. A proline substitution in the α-helix, found in mouse LPL, is expected to interfere with several hydrogen bonds, explaining why 5D2 cannot bind to mouse LPL.

Thyroglobulin (Tg) is a secreted iodoglycoprotein serving as the precursor for T3 and T4 hormones. Many characterized Tg gene mutations produce secretion-defective variants resulting in congenital hypothyroidism (CH). Tg processing and secretion is controlled by extensive interactions with chaperone, trafficking, and degradation factors comprising the secretory proteostasis network. While dependencies on individual proteostasis network components are known, the integration of proteostasis pathways mediating Tg protein quality control and the molecular basis of mutant Tg misprocessing remain poorly understood. We employ a multiplexed quantitative affinity purification–mass spectrometry approach to define the Tg proteostasis interactome and changes between WT and several CH-variants. Mutant Tg processing is associated with common imbalances in proteostasis engagement including increased chaperoning, oxidative folding, and engagement by targeting factors for ER-associated degradation (ERAD). Furthermore, we reveal mutation-specific changes in engagement with N-glycosylation components, suggesting distinct requirements for one Tg variant on dual engagement of both oligosaccharyltransferase complex isoforms for degradation. Modulating dysregulated proteostasis components and pathways may serve as a therapeutic strategy to restore Tg secretion and thyroid hormone biosynthesis.

Attorney General Kathy Jennings announced Thursday that Delaware and other states are suing search giant Google LLC for anticompetitive conduct in violation of Section 2 of the Sherman Act. The suit comes one week after Delaware announced a lawsuit against Facebook Inc. for violations of the Sherman Act and Clayton Act. “Google became a household […]