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By Keegan Kelly Published: November 12th, 2024

This is how mentally deranged liberals are… a Democrat in Minnesota was so filled with rage that Donald Trump won the election last week that he murdered his own wife and kids and then committed suicide to prevent them all from having to live during the next Trump presidency. Notice how you never heard any […]

The post Lunatic Democrat Murders His Wife and Kids Over His Hate for Donald Trump appeared first on The Lid.

The Cape South African Jewish Board of Deputies has asked the Equality Court in Cape Town to declare politician Mehmet Vefa Dag's social media posts about about Jewish people hate speech.

The following article, Lunatic Democrat Murders Wife and Kids, Commits Suicide Over His Hate for Donald Trump, was first published on Conservative Firing Line.

This is how mentally deranged liberals are… a Democrat in Minnesota was so filled with rage that Donald Trump won the election last week that he murdered his own wife and kids and then committed suicide to prevent them all from having to live during the next Trump presidency. Notice how you never heard any …

Continue reading Lunatic Democrat Murders Wife and Kids, Commits Suicide Over His Hate for Donald Trump ...

The inositol 1,4,5-trisphosphate (IP3) receptors (IP3Rs), which form tetrameric channels, play pivotal roles in regulating the spatiotemporal patterns of intracellular calcium signals. Mutations in IP3Rs have been increasingly associated with many debilitating human diseases such as ataxia, Gillespie syndrome, and generalized anhidrosis. However, how these mutations affect IP3R function, and how the perturbation of as-sociated calcium signals contribute to the pathogenesis and severity of these diseases remains largely uncharacterized. Moreover, many of these diseases occur as the result of autosomal dominant inheritance, suggesting that WT and mutant subunits associate in heterotetrameric channels. How the in-corporation of different numbers of mutant subunits within the tetrameric channels affects its activities and results in different disease phenotypes is also unclear. In this report, we investigated representative disease-associated missense mutations to determine their effects on IP3R channel activity. Additionally, we designed concatenated IP3R constructs to create tetrameric channels with a predefined subunit composition to explore the functionality of heteromeric channels. Using calcium imaging techniques to assess IP3R channel function, we observed that all the mutations studied resulted in severely attenuated Ca2+ release when expressed as homotetramers. However, some heterotetramers retained varied degrees of function dependent on the composition of the tetramer. Our findings suggest that the effect of mutations depends on the location of the mutation in the IP3R structure, as well as on the stoichiometry of mutant subunits assembled within the tetrameric channel. These studies provide insight into the pathogenesis and penetrance of these devastating human diseases.

Eiji Kinoshita
Apr 1, 2006; 5:749-757
Technology

Myosins generate force and motion by precisely coordinating their mechanical and chemical cycles, but the nature and timing of this coordination remains controversial. We utilized a FRET approach to examine the kinetics of structural changes in the force-generating lever arm in myosin V. We directly compared the FRET results with single-molecule mechanical events examined by optical trapping. We introduced a mutation (S217A) in the conserved switch I region of the active site to examine how myosin couples structural changes in the actin- and nucleotide-binding regions with force generation. Specifically, S217A enhanced the maximum rate of lever arm priming (recovery stroke) while slowing ATP hydrolysis, demonstrating that it uncouples these two steps. We determined that the mutation dramatically slows both actin-induced rotation of the lever arm (power stroke) and phosphate release (≥10-fold), whereas our simulations suggest that the maximum rate of both steps is unchanged by the mutation. Time-resolved FRET revealed that the structure of the pre– and post–power stroke conformations and mole fractions of these conformations were not altered by the mutation. Optical trapping results demonstrated that S217A does not dramatically alter unitary displacements or slow the working stroke rate constant, consistent with the mutation disrupting an actin-induced conformational change prior to the power stroke. We propose that communication between the actin- and nucleotide-binding regions of myosin assures a proper actin-binding interface and active site have formed before producing a power stroke. Variability in this coupling is likely crucial for mediating motor-based functions such as muscle contraction and intracellular transport.

Switzerland-based reggae and dancehall recording artiste Cali P has expressed gratitude to the public for supporting his music, following the release of his latest single ' Secret Hater'. The song, which was produced jointly by himself and Suku...

Visual Abstract

α-Synuclein (α-Syn) is a protein implicated in the pathogenesis of Parkinson's disease (PD). It is an intrinsically disordered protein that binds acidic phospholipids. Growing evidence supports a role for α-Syn in membrane trafficking, including, mechanisms of endocytosis and exocytosis, although the exact role of α-Syn in these mechanisms is currently unclear. Here we investigate the associations of α-Syn with the acidic phosphoinositides (PIPs), phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2). Our results show that α-Syn colocalizes with PIP2 and the phosphorylated active form of the clathrin adaptor protein 2 (AP2) at clathrin-coated pits. Using endocytosis of transferrin as an indicator for clathrin-mediated endocytosis (CME), we find that α-Syn involvement in endocytosis is specifically mediated through PI(4,5)P2 levels on the plasma membrane. In accord with their effects on PI(4,5)P2 levels, the PD associated A30P, E46K, and A53T mutations in α-Syn further enhance CME in neuronal and nonneuronal cells. However, lysine to glutamic acid substitutions at the KTKEGV repeat domain of α-Syn, which interfere with phospholipid binding, are ineffective in enhancing CME. We further show that the rate of synaptic vesicle (SV) endocytosis is differentially affected by the α-Syn mutations and associates with their effects on PI(4,5)P2 levels, however, with the exception of the A30P mutation. This study provides evidence for a critical involvement of PIPs in α-Syn–mediated membrane trafficking.

The statewide email to teachers is just the latest chapter in a rocky relationship between Kentucky officials and teachers.

Richard Saul Wurman, the founder of the popular speaker series, shares his ideas for how to make learning more interesting

Volker (OM Montenegro) describes how their outdoor lessons for Roma kids were threatened by bad weather. Then God provided not only the solution - building a carport as a shelter - but also the funds and manpower needed to build it.

Defendant convicted of racist harassment of Governor’s employee The Attorney General’s Division of Civil Rights and Public Trust (DCRPT) secured a historic guilty verdict in a felony hate crimes case, Attorney General Kathy Jennings announced Thursday. A New Castle County jury convicted Defendant Matthew Gregg of Hate Crimes, Harassment, and Terroristic Threatening, for repeatedly verbally […]

A Sussex County man has been sentenced to two years in prison for a hate crime involving a neighbor. James Raab, of Laurel, was sentenced on April 22 after pleading guilty to felony Hate Crime, Possession of a Deadly Weapon During the Commission of a Felony, Aggravated Menacing, and Resisting Arrest. Raab, 38, was arrested […]

Division of Civil Rights and Public Trust worked with UD Police Department  A University of Delaware student, who has since been banned from campus, now faces three misdemeanor charges, including Hate Crime, after vandalizing a Holocaust memorial sponsored by a Jewish student group and going on an antisemitic tirade. On the evening of May 8, […]

Check here each week to keep up with the latest from John MacArthur's pulpit at Grace Community Church.

[Ghanaian Times] The Chairperson of the National Commission for Civic Education (NCCE), Ms Kathleen Addy, has cautioned Ghanaians against hate speech as it could cause conflict in the lead-up to the General Election.

Scripture isn’t shy about the unpopularity of the gospel. The message of the cross is called “foolishness” and a “stumbling block” (1 Corinthians 1:23). Christ Himself is called “a rock of offense” (Romans 9:33). Jesus simply told His followers that the world would hate them (John 15:18–20). But what makes the gospel so repulsive to the world?

Friends who blow hot and cold put more strain on your physical and mental health than enemies. Here's how to spot them and handle them

Or, for the other half of you: Here Is Our Ivy Wolk Explainer.

Dallas Mavericks minority owner Mark Cuban returned to the Bluesky social media platform with a post after weeks of contentious X posts.

LEBRON James took a social media hiatus throughout the NBA playoffs. Now that he’s king again, he unloaded perfectly.

SURFING legend Kelly Slater has revealed how miserable he was starring alongside Pamela Anderson on Baywatch.

Monster and Brandwatch break down exactly how Americans are feeling about their jobs.

I can not believe the bellyachin' from these kids. "But Bill, we better stay indoors where it's safe", "but Bill, governor says stay away from winders"! That dog won't hunt, son.

“There can be no middle ground, not in this moment.” As the U.S. presidential race draws to a close, Bishop William Barber, the national co-chair of the Poor People’s Campaign, founding director of the Center for Public Theology and Public Policy at Yale Divinity School and co-author of White Poverty: How Exposing Myths About Race and Class Can Reconstruct American Democracy, explains why he is endorsing Kamala Harris for president in his personal capacity. In contrast to Donald Trump’s divisive rhetoric and policies that will benefit the rich, Barber says “we see clearly Harris trying to unify.” He makes a theological argument for opposing Trump and also discusses voting rights and access in his home state of North Carolina.

Donald Trump has been reelected president of the United States. Ahead of Kamala Harris’s expected concession speech, we speak to professors Carol Anderson and Michele Goodwin to discuss Harris’s historic campaign — and historic loss. “The Confederacy won,” says Anderson, a professor of African American studies at Emory University. “It paints a picture of what Americans are willing to embrace,” says Goodwin, a professor of constitutional law at Georgetown and an expert on healthcare law, who warns of the public health dangers of a second Trump administration and discusses the election’s implications for reproductive rights.

The FBI is investigating a spate of racist text messages targeting Black Americans in the wake of Donald Trump’s election victory last week. The texts were reported in states including Alabama, North Carolina, Pennsylvania and Virginia, addressing recipients as young as 13 by name and telling them they were “selected to pick cotton at the nearest plantation” and other messages referencing slavery. For more, we speak with Robert Greene II, a history professor at Claflin University, South Carolina’s first and oldest historically Black university in Orangeburg, where many students were targeted. “Initially when I heard about the texts, I thought it was a bit of a hoax, but … it quickly became clear that this wasn’t just a Claflin problem, it was a national issue, as well,” says Greene. We also speak with Wisdom Cole, senior national director of advocacy for the NAACP, who says “this is only the beginning,” with a second Trump administration expected to attack civil rights and embolden hate groups.

President-elect Donald Trump reportedly plans to appoint his former senior adviser Stephen Miller as his deputy chief of staff for policy. Miller will play a key role along with Trump’s border czar Tom Homan and South Dakota Governor Kristi Noem, who will reportedly be the secretary of the Department of Homeland Security. Miller is the architect of Trump’s anti-immigrant agenda, an avowed white nationalist and a man who is spurred by his “animus to the notion of the United States as a multicultural and multiethnic democracy,” says author Jean Guerrero, author of Hatemonger: Stephen Miller, Donald Trump, and the White Nationalist Agenda. Guerrero says the Trump administration’s “obsessive deportation” attempt to “radically reengineer the racial demographics of the United States” will “backfire” on the U.S. economy and destroy “the United States’ global reputation as a safe haven for the persecuted.”