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Components in the mosquito saliva can trigger an unexpected and long-lasting immune responses -- up to seven days post-bite, say scientists.

The researchers found that more than 100 proteins in mosquito saliva are mediating the effects on the immune system, or may help the virus become more infectious.

Identifying these proteins could help design strategies to fight transmission of dengue fever as well as other diseases caused by viruses also transmitted by Aedes aegypti, such as Zika virus, chikungunya virus and yellow fever virus, the researchers said.

"We found that mosquito-delivered saliva induced a varied and complex immune response we were not anticipating," said Silke Paust, Assistant Professor at Baylor and Texas Children's Hospital.

"Billions of people worldwide are exposed to diseases transmitted by mosquitoes, and many of these conditions do not have effective treatments," added Rebecca Rico-Hesse, Professor at the Baylor College of Medicine in Texas, US.

For the study, appearing in the journal PLOS Neglected Tropical Diseases, the team worked with a mouse model of the human immune system.

Previously, the team demonstrated that mosquito-bite delivery and needle-injection delivery of dengue virus in these "humanised mice" led to significantly different disease developments

They found that mosquitoes are not just acting like "syringes" to merely inject viruses, but their saliva seems to contribute significantly to the development of the disease.

In the new study, the team tested the effect of virus-free mosquito saliva on humanised mice and compared the results with those obtained from humanised mice that had not been bitten by mosquitoes.

Evidence to immune responses -- up to seven days post-bite -- was found in multiple tissue types, including blood, skin and bone marrow, the researchers said.

"For instance, both the immune cell responses and the cytokine levels were affected. We saw activation of T helper cells 1, which generally contribute to antiviral immunity, as well as activation of T helper cells 2, which have been linked to allergic responses," Paust said.

"The diversity of the immune response was most striking to me. This is surprising given that no actual infection with any type of infectious agent occurred," he noted.

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Filmmaker James Gunn recently revealed that neither of his upcoming movie -- 'Guardians of the Galaxy, Vol 3 nor 'The Suicide Squad' will be affected by delays due to the coronavirus crisis.

One of the director's fans in a question-answer session on the Twitter asked about the possibilities of pushing ahead 'Guardians of the Galaxy' vol 3, to which Gunn on Sunday replied: "Right now the plans with Vol 3 are also exactly the same as they were before coronavirus."

In another reply, the 53-year-old said: "Right now there's no reason for #TheSuicideSquad release date to move. We are on or ahead of schedule. We were extremely fortunate to wrap shooting & set up editing from our homes (due to a post-production team & studio with foresight) before quarantine."

The upcoming movie is a sequel to the original 2016 released 'Suicide Squad.'

Actors Margot Robbie, Joel Kinnaman, and Viola Davis will reprise their roles in the film alongside new cast members Taika Waititi, Peter Capaldi, Idris Elba, John Cena, Storm Reid, Pete Davidson, David Dastmalchian and Daniela Melchior.

The original 'Suicide Squad' was a box office success, grossing USD 746.8 million worldwide. The upcoming fantasy-action is expected to hit theatres on August 6, 2021.

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Cristiano Ronaldo and his Portugal teammates have donated half of their bonus for qualifying for the Euro 2020 finals to help amateur football in the country that has been hit by the coronavirus shutdown. The Portuguese football federation said on Monday the money would go towards a fund that it hopes will reach 4.7 million euros ($5.1 million) to help amateur clubs survive the crisis.

The federation last week decided to end the already-suspended amateur season with immediate effect, declaring the season null and void without champions, promotion or relegation. No decision has been taken on resuming professional football but the president of the Portuguese league said in late March he was determined the season should be completed.

"It is fundamental that the championships end because it would allow some sort of normality to be restored and we could prepare for next season knowing who has qualified for international competitions, who the champions are and who is relegated," Pedro Proenca said. Portugal shocked host nation France to win Euro 2016 but Ronaldo and his teammates will have to wait another year to defend their title as Euro 2020 has been postponed by 12 months because of the coronavirus pandemic.

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Pakistan's Coronavirus cases jumped to 12,579 on Sunday after 783 new infections were reported in one day, prompting the government officials and medical experts to appeal to the people to avoid visiting mosques and participating in congregational prayers during Ramzan to contain the spread.

According to the Ministry of National Health Services, at least 15 people have died in the last 24 hours, taking the death toll in the country to 269. The number of those recovered also increased to 2,866, it said. So far, 144,365 tests have been done in the country, including 6,218 in the last 24 hours.

As the infections keep growing, Pakistan Medical Association, Pakistan Islamic Medical Association (PIMA) have urged people to offer prayers at homes and not in mosques. PIMA President Dr Iftikhar Burney warned on Saturday that mosques were becoming a major source of virus transmission.

"Around 6,000 cases for coronavirus surfaced in a month, but the same has doubled in the last six days," he said, warning that the infection would further go up in the coming months of May and June.

The are reports that a 20-point agreement signed by the leading clerics with President Arif Alvi on restricting access to mosques during Ramzan was not being followed completely. Alvi has written a letter to the Imams of mosques urging them to ask worshippers above the age of 50 to pray at home.

783
No. of new infections reported on Sunday

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Dengue virus can be eliminated by injecting Wolbachia bacteria in mosquitoes. Researchers are still finding ways to spread Wolbachia in an urban mosquito population.

Asian tiger mosquito has been neglected as a source of Zika and dengue virus, as the threat was measured right after one feeding on infected blood. However,

Using CRISPR/Cas9, a gene editing tool gene, the FREP1 gene can be inactivated to reduce mosquitoes vulnerability to Plasmodium parasite, a genus that causes malaria in humans.

Mosquito bites now could be prevented using clothes made up of the nanomaterial graphene, which is also a material actively used is a lot of products from solar cells to tennis rackets.

Releasing mosquitoes infected with a type of bacteria could wipe out the spread of dengue, thereby reducing global dengue cases by 90 percent, reports a new study.

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This country fact-sheet presents key figures from "Under Pressure: The Squeezed Middle Class". This report analyses the trends of middle-income households in areas such as employment, consumption, wealth and debt, as well as perceptions and social attitudes. It also includes recommendations for protecting middle-class living standards and financial security in the face of economic challenges.

This country fact-sheet presents key figures from "Under Pressure: The Squeezed Middle Class". This report analyses the trends of middle-income households in areas such as employment, consumption, wealth and debt, as well as perceptions and social attitudes. It also includes recommendations for protecting middle-class living standards and financial security in the face of economic challenges.

Small and medium-sized businesses (SMEs) requesting loans between 2007 and 2010 faced higher interest rates than for large companies. Loan conditions for SMEs included shortened maturities and increased demands for collateral, suggesting that banks considered smaller firms to be a higher risk.

This country fact-sheet presents key figures from "Under Pressure: The Squeezed Middle Class". This report analyses the trends of middle-income households in areas such as employment, consumption, wealth and debt, as well as perceptions and social attitudes. It also includes recommendations for protecting middle-class living standards and financial security in the face of economic challenges.

This country fact-sheet presents key figures from "Under Pressure: The Squeezed Middle Class". This report analyses the trends of middle-income households in areas such as employment, consumption, wealth and debt, as well as perceptions and social attitudes. It also includes recommendations for protecting middle-class living standards and financial security in the face of economic challenges.

This country fact-sheet presents key figures from "Under Pressure: The Squeezed Middle Class". This report analyses the trends of middle-income households in areas such as employment, consumption, wealth and debt, as well as perceptions and social attitudes. It also includes recommendations for protecting middle-class living standards and financial security in the face of economic challenges.

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