The 700-bp A homology arm (A-box) and the 700-bp B homology arm (B-box) are amplified by polymerase chain reaction (PCR) using purified bacterial artificial chromosome (BAC) DNA as template for two-step BAC engineering. The resulting A-box PCR product contains an AscI site at its 5' end (the 5' primer incorporates an AscI site, and the 3' primer does not incorporate any restriction sites). The B-box PCR product contains an XmaI site at its 3' end (the 5' primer does not incorporate any restriction sites, and the 3' primer incorporates an XmaI site). The amplification products are then digested with the appropriate restriction endonucleases to render them suitable for cloning into the shuttle vector.

With Mother's Day only a few days away, businesses are coming up with creative ways to help your family celebrate. Everything from brunch in a box to colourful bouquets and home delivered chocolate.

The Xenopus oocyte is a unique model system, allowing both the study of complex biological processes within a cellular context through expression of exogenous mRNAs and proteins, and the study of the cell, molecular, and developmental biology of the oocyte itself. During oogenesis, Xenopus oocytes grow dramatically in size, with a mature oocyte having a diameter of ~1.3 mm, and become highly polarized, localizing many mRNAs and proteins. Thus, the mature oocyte is a repository of maternal mRNAs and proteins that will direct early embryogenesis prior to zygotic genome transcription. Importantly, the Xenopus oocyte also has the capacity to translate exogenous microinjected RNAs, which has enabled breakthroughs in a wide range of areas including cell biology, developmental biology, molecular biology, and physiology. This introduction outlines how Xenopus oocytes can be used to study a variety of important biological questions.

In SOLIDWORKS Electrical, we have the capability to create symbols with defined circuits on the fly, or for use with connectors that entail many, many pins. These are traditionally called Black Boxes. See figure 1 & 2.    Figure 1

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Hi, I’m Mike Dady, Application Engineer for Alignex. As many of you probably know, the Toolbox Add-In is a database that allows for the quick addition of fasteners and other hardware to a SOLIDWORKS Assembly. However, many don’t realize that

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IF ever we needed to indulge in a bout of collective escapism, if only for 60 minutes on a Sunday night, then that time is now. Thankfully ITV has just the thing – Belgravia, the latest big budget costume drama from Downton Abbey creator Julian Fellowes and a series that positively ripples with dimples, quiffs, honey-coloured chiffon frocks, eye-popping millinery and glamorous uniforms.

NEATEBOX, the Scottish technology firm which specialises in improving accessibility for people with disabilities, has been accepted into an accelerator programme run by Royal Bank of Scotland.

Microsoft rolls out a major update to its Xbox game Pass Quests system including 90 more Quests as well as daily, weekly, and monthly challenges.

Microsoft notes it can pay bug bounty participants more than $20,000, depending on the vulnerability's severity and the report's quality. Just make sure you don't use the flaw on unsuspecting users.

With Box Zones, Box customers can use Amazon Web Services and IBM Cloud to store data overseas.

All this foreign involvement has failed to prevent the recent coup or stabilize its aftermath. BINUCA has not been able to implement a disarmament, demobilisation and reintegration program, and it failed to convince Bozizé’s regime to reform the security sector or consolidate the peace. ECCAS has been unable to restore order in one of the smallest capitals of Africa, and troop-contributing countries have proved unable to deliver the 600 extra soldiers they committed to provide in April. Paradoxically, France, while securing Bangui’s airport, is also hosting ousted president Bozizé, who declared from exile in Paris his wish to retake power by force with the “support” of private actors.

Baloxavir marboxil, a prodrug of cap-dependent endonuclease inhibitor, baloxavir acid, reduces the time to improvement of influenza symptoms in patients infected with type A or B influenza virus. To characterize its pharmacokinetics, a population pharmacokinetic model for baloxavir acid was developed using 11846 plasma concentration data items from 1827 subjects including 2341 plasma concentration data items from 664 patients at high risk of influenza complications. A three-compartment model with first-order elimination and first-order absorption with lag time well described the plasma concentration data. Body weight and race were found to be the most important factors influencing clearance and volume of distribution. The exposures in high-risk patients were similar to those in otherwise healthy patients, and no pharmacokinetic difference was identified regarding any risk factors for influenza complications.

Exposure-response analyses were performed regarding the time to improvement of symptoms and the reduction in the influenza virus titer in high-risk patients. The analyses suggested that body weight-based dosage, 40 mg for patients weighing < 80 kg and 80 mg for patients weighing ≥ 80 kg, can shorten the time to improvement of influenza symptoms and reduce virus titer for both type A and B influenza virus regardless of the exposure levels of the high-risk patients as well as for the otherwise healthy influenza patients.

The results of our population pharmacokinetic and exposure-response analyses in patients with risk factors of influenza complications should provide useful information on the pharmacokinetic and pharmacodynamic characteristics of baloxavir marboxil and also for the optimization of dose regimens.

Using their unique skills and experiences, three LaunchBox grant recipients are providing a variety of COVID-19 relief efforts.

Effective immediately and for the foreseeable future, the Entrepreneurship and Assistance Clinic is expanding its scope and service offerings to small Pennsylvania businesses, in response to the COVID-19 pandemic.

A new high-res audio player looks like a wooden flask and has a tie-up with Tidal.

Qualcomm's new home Internet box will grab 5G from towers a mile from your house.

The Great Valley LaunchBox, funded by Invent Penn State, will provide mentoring services, office hours, workshops, speakers series and more to the local entrepreneurial community.

The first program, focused on Small Business, will be held on May 13.

Multi-state settlement resolves allegations of improper marketing and sale of addictive withdrawal treatment Attorney General Kathy Jennings announced today that Delaware and other states have reached an agreement with pharmaceutical distributor Reckitt Benckiser Group (“Reckitt”) to settle allegations that the company, either directly or through a subsidiary, improperly marketed and otherwise promoted the drug Suboxone, […]

A driver of a Porsche Boxster was forced to do squats while holding his ears by Indore Police when he ventured out in his convertible sportscar during the lockdown.

Xiaomi Mi 10, Mi True Wireless Earphones 2, and Mi Box will be launched in India today via an online-only keynote event.

Xiaomi says the Mi Box 4K has been designed to turn any regular feature TV into a smart TV, sort of like the Amazon Fire TV Stick 4K.

The Mi Box 4K, like every other Xiaomi product out there, also punches way above its weight when it comes to feature set and pricing.

Bring some 'oomph' to your audience with these simple content hacks

Xiaomi had a massive announcement today in India - the company finally introduced the Mi 10 smartphone, the Mi True Wireless Earphones 2, and also unveiled the Mi Box 4K - an Android TV gadget for streaming video content with voice-assisted remote control. The Xiaomi Mi 10 comes with Snapdragon 865 chipset and 8 GB RAM, while storage is either 128 GB or 256 GB. The price is INR49,999 ($660/€610) or INR54,999 ($727/€672), but some fans might be disappointed to learn that the Mi 10 Pro won't be arriving in India for now. They are left with the vanilla version that has a 108 MP main...

While he says he has no interest in facing a top-ranked boxer, Floyd Mayweather is refusing to rule out a fight with the likes of Conor McGregor or Khabib Nurmagomedov, as long as the money is right.
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  Google has introduced an easy way to handle your email with a revamped inbox in Gmail. In its new avatar, Gmail inbox provides a hassle-free way to manage, sort and organize your email. Email inboxes...

An investigation has been launched in Idaho after a video of a woman 'boxing' a dog went viral online, sparking outrage among viewers.

The TCL 10 Pro has finally landed, delivering a mid-range device with a sleek design and an impressive camera setup. The main highlight of the phone is its curved 6.47 FHD+ AMOLED display which is not that common when looking at mid-range devices like this. Powering the phone is a Snapdragon 675 with 6GB of ...