surface

Methods for orienting superabrasive particles on a surface and associated tools

Methods of making a superabrasive tool precursor are disclosed, along with such precursors and associated tools. Particularly, methods are disclosed for orienting superabrasive particles in a viscous binding material in order to provide tools based thereupon and having desired performance characteristics.




surface

DETERMINING A TOPOLOGY OF THE SURFACE OF A MATERIAL FILLED INTO A CONTAINER

A fill level measurement device for determining a topology of a filling material surface in a container including an antenna apparatus, a receiver and control circuitry. An emission angle of the antenna apparatus and a spatial position of the antenna apparatus relative to the filling material surface is settable by the control circuitry. A resultant emission direction of the fill level measurement device is changeable by the control circuitry by controlling a position of the antenna apparatus and by controlling an emission and/or receiving angle of the antenna apparatus.




surface

COMPOSITIONS FOR REPAIRING DEFECTS IN SURFACE COVERINGS

Described herein are putty compositions comprising: an initiator component comprising a thermal initiator and a photo initiator; an acrylate-functional resin; and a filler composition comprising: calcium carbonate particles; and glidant particles; wherein the calcium carbonate particles and the glidant particles are present in a weight ratio ranging from about 4:1 to about 1:1.




surface

THERMAL SPRAY COATINGS ONTO NON-SMOOTH SURFACES

This invention relates to thermal spray coatings and processes onto non-smooth surfaces. The coating and processes can coat non-smooth surfaces without substantial degradation of the underlying surface texture or profile of the non-smooth surfaces so as to sufficiently preserve the underlying surface texture or profile. The ability for coating fractional coverage to maintain the surface profile while maintaining wear resistance is unprecedented by conventional thermal spray processes




surface

ELASTOMERIC COATING ON A SURFACE

An example composite material composed of a metal or multilayered metal substrate onto which an elastomer or elastomeric mixture is positioned by electrophoretic disposition. The elastomer or elastomeric mixture forms a flexible non-rigid elastomeric coating, which imparts a soft touch tactile feeling to the metal base substrate.




surface

METHOD FOR FORMING ORGANIC COATING ON COPPER SURFACE

A method for selective deposition of an organic solderability preservative coating on a copper surface of an article is disclosed. The method includes two steps of organic coatings by two solutions; the first step contains contacting the copper surface with a first solution including azole compound and the second step contains contacting the copper surface treated by the first solution with a second solution including a specific pyrazine derived compound.




surface

SURFACE-TREATED COPPER FOIL, METHOD FOR PRODUCING SAME, COPPER-CLAD LAMINATE FOR PRINTED WIRING BOARD, AND PRINTED WIRING BOARD

There is provided a surface-treated copper foil including a surface coating layer provided on at least one surface of a copper foil, the surface coating layer being mainly composed of silicon with a hydrogen content of 1 to 35 atomic % and/or a carbon content of 1 to 15 atomic %. This foil can be manufactured by forming a surface coating layer composed mainly of silicon with the above hydrogen and carbon contents on at least one surface of the copper foil by PVD or CVD. The present invention can provide a copper foil with a surface coating layer that can achieve a high bonding strength to a resin layer even if the copper foil has an extremely smooth surface such as one formed by vapor deposition, for example, sputtering and also has a desirable insulation resistance suitable for achieving a fine pitch in a printed wiring board.




surface

MULTI-SIDED HEAT EXCHANGERS WITH COMPLIANT HEAT TRANSFER SURFACES

A heat exchanger for a battery has fluid-carrying panels and defines a multi-sided enclosure for enclosing at least two sides of the battery. The heat exchanger has first and second fluid-carrying panels defining first and second flow channels, where the first and second fluid-carrying panels are arranged at an angle to another. The heat exchanger may also include a third fluid-carrying panel defining a third flow channel, and being arranged at an angle to the second fluid-carrying panel. The heat exchanger has first and second plates sealingly joined together along their peripheries and defining a fluid flow passageway between their central fluid flow areas. The second plate may be compliant, its central fluid flow area being deformable away from the central fluid flow area of the first plate in response to a pressure of a fluid inside the fluid flow passageway.




surface

CRYOGENIC HEAT TRANSFER BY A NANOPOROUS SURFACE

Various methods and systems are provided for cryogenic heat transfer by nanoporous surfaces. In one embodiment, among others, a system includes a cryogenic fluid in a flow path of the system; and a system component in the flow path that includes a nanoporous surface layer in contact with the cryogenic fluid. In another embodiment, a method includes providing a cryogenic fluid; and initiating chilldown of a cryogenic system by directing the cryogenic fluid across a nanoporous surface layer disposed on a surface of a system component.




surface

Process for producing dispersion of surface-treated carbon black powder, and process for producing surface-treated carbon black powder

A method of producing a surface-treated carbon black powder dispersion includes subjecting carbon black fine particles having a volume average particle size of 100 nm to 20 μm to wet granulation and drying by heating to obtain granulated carbon black having a hardness of 12 cN or less and a pH of less than 7, grinding the granulated carbon black to obtain a ground product having a volume average particle size of 20 nm to 20 μm, and subjecting the ground product to wet oxidization in an aqueous medium. The resulting surface-treated carbon black powder dispersion exhibits excellent print density, print quality, discharge stability, and storage stability when used as an inkjet printer aqueous black ink.




surface

Surface treatment device

A device used to smooth a material's surface with abrasion. More specifically, the device is in the form of a glove or mitten with an abrasive layer that is placed over, and secured on, a user's hand to protect the user's hand and to provide an agile and flexible range of motion while sanding a material's surface.




surface

Surface covering kit comprising panels and an extraneous locking element

A surface covering kit is disclosed, comprising a plurality of generally rectangular panels (100). Each panel is provided with grooves (301, 302, 301', 302') forming an insertion channel (303) when two panels (100) are laid next to each other along their transverse sides (105, 105') in a common laying plane (101). An extraneous locking element (210) is received by the insertion channel (303), the extraneous locking element (210) having a longitudinal dimension and a cross-section perpendicular to said longitudinal dimension which forms a cross with at least four cross arms. The corresponding cross arms of the insertion channel have two parallel flank surfaces (1603, 1604) each, and each of the cross arms is oriented diagonally in a plane perpendicular to the panel plane (102).




surface

Unitary safety surface tiles and associated structures

A unitary safety surface tile is provided. The tile may interlock with at least one other tile. The tiles may be configured for disposing on a base of loose fill and for receiving a covering of loose fill. The tiles may include a plurality of hollow inverted pyramidal shaped pockets. Each of the pockets may connect to at least two other pockets along a top edge of each pocket. Each of the pockets may engage the base of loose fill and/or receive the covering of loose fill. Each of the pockets may include one or more apertures for allowing passage of fluid through each of the pockets. Each tile may further include a plurality of edges. The edges may border the plurality of pockets. Each of at least two of said plurality of edges may be adapted for engaging at least one edge of at least one other tile.




surface

BROADBAND SURFACE SCATTERING ANTENNAS

A surface scattering antenna with a tightly-coupled or tightly-connected array of radiators provides an adjustable antenna with broadband instantaneous bandwidth.




surface

MICROFLUIDIC APPARATUS HAVING AN OPTIMIZED ELECTROWETTING SURFACE AND RELATED SYSTEMS AND METHODS

Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section or module having a dielectrophoresis configuration; systems that include any of the microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the medium of the aqueous droplet; related kits; and methods of manipulating droplets, optionally containing micro-objects such as biological cells, within the microfluidic devices.




surface

METHOD OF SPUTTER DEPOSITION OF A FILM ON AN ESSENTIALLY PLANE EXTENDED SURFACE OF A SUBSTRATE

A film is sputter-deposited on an essentially plane, extended surface of a substrate which has recesses therein, namely at least one of grooves, of holes, of bores, of vias, of trenches. So as to establish on one hand a homogeneous thickness distribution of the film along the addressed surface of the substrate and, on the other hand, a thick film deposition within the recesses, sputter deposition is performed first at a large distance between a sputter surface of a target and the addressed surface of the substrate and then at a reduced distance between the addressed surfaces.




surface

QUALITY EVALUATION METHOD FOR LAMINATE HAVING PROTECTIVE LAYER ON SURFACE OF OXIDE SEMICONDUCTOR THIN FILM AND QUALITY CONTROL METHOD FOR OXIDE SEMICONDUCTOR THIN FILM

Provided is a method for simply evaluating defects caused in interface states in oxide semiconductor thin films and protective films in TFTs having protective films formed on the surface of oxide semiconductor thin films without actually measuring the characteristics of the same. This evaluation method evaluates defects caused in the interface states by measuring electron states in the oxide semiconductor thin film by a contact method or noncontact method. The defects caused in the interface states are any of the following (1)-(3). (1) Threshold value voltage (Vth,) when a positive bias is applied to the thin-film transistor(2) Difference in threshold value voltage (ΔVth) before and after applying the positive bias to the thin-film transistor(3) Threshold value during the first measurement when a plurality of measurements is made of the threshold value voltage when a positive bias is applied to the thin-film transistor.




surface

DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR

Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.




surface

SURFACE MACHINING METHOD FOR SINGLE CRYSTAL SIC SUBSTRATE, MANUFACTURING METHOD THEREOF, AND GRINDING PLATE FOR SURFACE MACHINING SINGLE CRYSTAL SIC SUBSTRATE

A surface machining method for a single crystal SiC substrate, including: a step of mounting a grinding plate which includes a soft pad and a hard pad sequentially attached onto a base metal having a flat surface, a step of generating an oxidation product by using the grinding plate, and a step of grinding the surface while removing the oxidation product, wherein abrasive grains made of at least one metallic oxide that is softer than single crystal SiC and has a bandgap are fixed to the surface of the hard pad.




surface

Fiberboard Surface Protection System

A paperboard or fiberboard sheet is provided with at least one preapplied adhesive strip used to connect together and an adjacently positioned surface protector to form a dust-proof and/or liquid-proof seal between the two surface protectors.




surface

Tyra Banks Slammed After Old 'ANTM' Clip of Her Telling Off a Model Over Gap Tooth Resurfaces

In the video, Tyra advises contestant Danielle Evans to close the gap in her front teeth, saying, 'Do you really think you can a have a covergirl contract with a gap in your mouth?'



  • tv
  • America's Next Top Model

surface

Dynamic Controls 2 turns ROLI Lightpad Block into customizable MIDI control surface

The Dynamic Controls app for ROLI Dashboard turns a ROLI Lightpad Block into a highly customizable MIDI control surface, allowing you to design your own control layout using Buttons, Faders and XY Pads. Connect your Lightpad Block to the computer and launch ROLI Dashboard to load the Dynamic Controls app. Use Dashboard to edit the […]

The post Dynamic Controls 2 turns ROLI Lightpad Block into customizable MIDI control surface appeared first on rekkerd.org.




surface

Scottsdale Community College apologizes after 'inappropriate' questions about Islam surface on quiz

Questions implying that terrorism is encouraged under the Islamic faith elicited a public apology from Scottsdale Community College after they appeared on a class quiz.

       




surface

'The terror was brought to us': Memories of Oka resurface as rail blockade crisis continues

Thirty years after she was wounded during the clash between soldiers and Mohawk activists at Oka, Que., ex-Olympian Waneek Horn-Miller reflects on how the country has changed — and how the rail blockade crisis could end badly.



  • Radio/The House

surface

Power outage 'resurfaces' impacting businesses and customers in section of Adelaide CBD

A power outage in Adelaide has resurfaced hours after it had been resolved, impacting businesses and customers in the busiest section of the Adelaide CBD. The cause is still being investigated by authorities.




surface

Video surfaces of elderly man crawling in pain after being refused MRI scan at Adelaide hospital

The family of an elderly man posts a video of him crawling on his hands and knees to the toilet and claims he was discharged from an Adelaide hospital without proper treatment.




surface

Archaeologists look below the ocean surface in Dampier Archipelago for signs of ancient culture

A pioneering project on the WA coast is searching for underwater evidence of ancient culture. Scientists hope the exploration will lead to evidence of human life from when the seabed was land.



  • ABC Radio Adelaide
  • adelaide
  • northwestwa
  • Community and Society:History:All
  • Community and Society:Indigenous (Aboriginal and Torres Strait Islander):Aboriginal
  • Community and Society:Indigenous (Aboriginal and Torres Strait Islander):Indigenous Culture
  • Environment:Oceans and Reefs:All
  • Science and Technology:Archaeology:All
  • Science and Technology:Earth Sciences:All
  • Science and Technology:Geology:All
  • Science and Technology:Research:All
  • Australia:SA:Flinders University 5042
  • Australia:WA:Burrup 6714
  • Australia:WA:Dampier Archipelago 6713

surface

Lost tapes from Newcastle's Star Hotel riot resurface in new documentary series

Camera tapes, used by police to identify Star Hotel rioters 40 years ago, have resurfaced and show that fire hoses were used to subdue the crowds.



  • 1233 ABC Newcastle
  • newcastle
  • Arts and Entertainment:Film (Movies):Documentary
  • Arts and Entertainment:Music:All
  • Arts and Entertainment:Popular Culture:All
  • Community and Society:All:All
  • Community and Society:History:All
  • Information and Communication:Internet:Social Media
  • Law
  • Crime and Justice:Police:All
  • Unrest
  • Conflict and War:All:All
  • Australia:NSW:Newcastle 2300

surface

Deep diving with 'monsters, amazing alien animals' what's it like 100m below the ocean's surface?

Scuba divers can reach depths of 40 metres, but there's a small group of hardcore divers who venture more than twice as deep. You just need a sense of adventure and the right gear.





surface

Commuter Rail Div. v. Surface Transp. Bd.

(United States DC Circuit) - In a petition for review of the Surface Transportation Board's approval of the acquisition of certain railroads by another railroad, the petition is denied where: 1) one petitioner lacked standing because, if the board's decision were overturned, the construction authorization would not be affected and no new environmental impact statement would be required for the area; and 2) the board did not abuse its discretion in approving the transaction.




surface

Del Grosso v. Surface Transportation Board

(United States First Circuit) - Rejected a petition for review of a decision of the federal Surface Transportation Board (STB). The basic issue here was whether a local government could regulate certain activities at a rail-to-truck transloading facility. The petitioners, who lived near the facility, argued that the STB did not have exclusive jurisdiction and thus local regulation was permitted. Finding no basis to reverse the STB's ruling regarding the scope of its exclusive jurisdiction, the First Circuit rejected the petition for review.




surface

Pop Smoke’s Message In This Resurfaced Interview Is Chilling



He shared the thoughts in one of his final interviews.





surface

Subsurface Utility Engineering Information for Airports

TRB’s Airport Cooperative Research Program (ACRP) Synthesis 34: Subsurface Utility Engineering Information for Airports examines ways in which information on subsurface utilities is collected, maintained, and used by airports, their consultants, and the U.S. Federal Aviation Administration to help increase the effectiveness of, and enhance safety during, infrastructure development programs at airports. The report also compares the current state of technology and effective processes from other industry se...



  • http://www.trb.org/Resource.ashx?sn=coveracrpsyn034copy

surface

Maintenance cost savings for pavement surface treatments

Use of Surface Treatments to Extend Pavement Life: A Case Study on US 301, Sussex County, Virginia , released by the Virginia Department of Transportation




surface

TRB Webinar: How to Certify and Verify Pavement Surface Condition Data

Does your organization know how to measure and verify pavement surface condition data based on mapping sensor technologies? TRB will conduct a webinar on Wednesday, June 10, 2020, from 1:00 PM to 3:00 PM Eastern to discuss the challenges and solutions from experience on actual projects. In pavement management systems, accurate and precise condition data are essential for making data-driven decisions about maintenance and rehabilitation projects. Some condition data, including surface cracking and other d...




surface

Airport Surface Weather Observation Options for General Aviation Airports

The needs of airports may vary depending on the types of operations typically conducted at the airport, as well as the type of weather common to the airport. The TRB Airport Cooperative Research Program's ACRP Syntheis 105: Airport Surface Weather Observation Options for General Aviation Airports aims to provide the operators of general aviation (GA) airports a comprehensive source of information about airport-based weather observation options so they may make informed decisions to support the specific o...



  • http://www.trb.org/Resource.ashx?sn=cover_acrp_syn_105

surface

Utah Pulls Plug On Surveillance Contractor After CEO's Past As A White Supremacist Surfaces

A couple of months ago, a records request revealed a private surveillance contractor had access to nearly every piece of surveillance equipment owned and operated by the state of Utah. Banjo was the company with its pens in all of the state's ink. Banjo's algorithm ran on top of Utah's surveillance gear: CCTV systems, 911 services, location data for government vehicles, and thousands of traffic cameras.

All of this was run through Banjo's servers, which are conveniently located in Utah government buildings. Banjo's offering is of the predictive policing variety. The CEO claims its software can "find crime" without any collateral damage to privacy. This claim is based on the "anonymization" of harvested data -- a term that is essentially meaningless once enough data is collected.

This partnership is now on the rocks, thanks to an investigation by Matt Stroud and OneZero. Banjo's CEO, Damien Patton, apparently spent a lot of his formative years hanging around with white supremacists while committing crimes.

In grand jury testimony that ultimately led to the conviction of two of his associates, Patton revealed that, as a 17-year-old, he was involved with the Dixie Knights of the Ku Klux Klan. On the evening of June 9, 1990 — a month before Patton turned 18 — Patton and a Klan leader took a semi-automatic TEC-9 pistol and drove to a synagogue in a Nashville suburb. With Patton at the wheel, the Ku Klux Klan member fired onto the synagogue, destroying a street-facing window and spraying bullets and shattered glass near the building’s administrative offices, which were next to that of the congregation’s rabbi. No one was struck or killed in the shooting. Afterward, Patton hid on the grounds of a white supremacist paramilitary training camp under construction before fleeing the state with the help of a second Klan member.

If you're wondering where the state of Utah's due diligence is in all of this, there's a partial explanation for this lapse: the feds, who brought Patton in, screwed up on their paperwork.

Because Patton’s name was misspelled in the initial affidavit of probable cause filed in Brown’s case — an FBI agent apparently spelled Damien with an “o” rather than an “e” — any search of a federal criminal court database for “Damien Patton” would not have surfaced the affidavit.

Now that his past has been exposed, the state of Utah has announced it won't be working with Banjo.

The Utah attorney general’s office will suspend use of a massive surveillance system after a news report showed that the founder of the company behind the effort was once an active participant in a white supremacist group and was involved in the shooting of a synagogue.

The AG's office can only shut down so much of Banjo's surveillance software. Other government agencies not directly controlled by the state AG are making their own judgment calls. The University of Utah is suspending its contract with Banjo, but the state's Department of Public Safety has only gone so far as to "launch a review" of its partnership with the company. City agencies and a number of police departments who have contracts with Banjo have yet to state whether they will be terminating theirs.

And the AG's reaction isn't a ban. The office appears to believe it might be able to work through this.

“While we believe Mr. Patton’s remorse is sincere and believe people can change, we feel it’s best to suspend use of Banjo technology by the Utah attorney general’s office while we implement a third-party audit and advisory committee to address issues like data privacy and possible bias,” Piatt said. “We recommend other state agencies do the same.

It's refreshing to hear a prosecutor state that it's possible for former criminals to turn their lives around and become positive additions to their communities, but one gets the feeling this sort of forgiveness is only extended to ex-cons who have something to offer law enforcement agencies. Everyone else is just their rap sheet for forever, no matter how many years it's been since their last arrest.

The other problem here is the DA's office's tacit admission it did not take data privacy or possible bias into account before granting Banjo access to the state's surveillance equipment, allowing it to set up servers in government buildings, and giving it free rein to dust everything with its unaudited AI pixie dust.

These are all steps that should have taken place before any of this was implemented, even if the state had chosen to do business with a company with a less controversial CEO. This immediate reaction is the right step to take, but a little proactivity now and then would be a welcome change.





surface

Microsoft будет бесплатно чинить ноутбуки Surface Laptop 3 с треснутыми экранами

Компания обещает бесплатный ремонт устройств и возмещение расходов, если ноутбук уже отремонтировали.




surface

Don’t be shallow. A tale of subsurface microplastics and the processes that transport them.

One thing you should know about me is that I am from New York and I am half Italian. That means when I like something,…




surface

The moon isn't 'dead': Ridges on lunar surface show signs of recent tectonic activity

The moon isn't "dead" after all: Newly discovered ridges on the moon's surface are leading scientists to think that the moon might have an active tectonic system.




surface

Eli Broad's Malibu compound resurfaces at $75 million

The oceanfront home of billionaire philanthropist Eli Broad is for sale in Malibu at $75 million.




surface

BREAKING: New Docs Prove Obama Knew Details Of Flynn Wiretapping…Newly Surfaced Video Shows Obama Explaining How He Stays Out Of FBI Investigations

The following article, BREAKING: New Docs Prove Obama Knew Details Of Flynn Wiretapping…Newly Surfaced Video Shows Obama Explaining How He Stays Out Of FBI Investigations, was first published on 100PercentFedUp.com.

Barack Obama knew. Documents released yesterday that were used to exonerate President Trump’s new NSA General Flynn, prove that President Barack Obama was aware of the details of Michael Flynn’s intercepted phone calls on December 16 with then-Russian Ambassador Sergey Kislyak. On January 5, 2017, then-Deputy Attorney General, Sally Yates attended an Oval Office meeting […]

Continue reading: BREAKING: New Docs Prove Obama Knew Details Of Flynn Wiretapping…Newly Surfaced Video Shows Obama Explaining How He Stays Out Of FBI Investigations ...




surface

Perilipin is located on the surface layer of intracellular lipid droplets in adipocytes

EJ Blanchette-Mackie
Jun 1, 1995; 36:1211-1226
Articles




surface

Remnant lipoprotein metabolism: key pathways involving cell-surface heparan sulfate proteoglycans and apolipoprotein E

Robert W. Mahley
Jan 1, 1999; 40:1-16
Reviews




surface

Structural basis of cell-surface signaling by a conserved sigma regulator in Gram-negative bacteria [Molecular Biophysics]

Cell-surface signaling (CSS) in Gram-negative bacteria involves highly conserved regulatory pathways that optimize gene expression by transducing extracellular environmental signals to the cytoplasm via inner-membrane sigma regulators. The molecular details of ferric siderophore-mediated activation of the iron import machinery through a sigma regulator are unclear. Here, we present the 1.56 Å resolution structure of the periplasmic complex of the C-terminal CSS domain (CCSSD) of PupR, the sigma regulator in the Pseudomonas capeferrum pseudobactin BN7/8 transport system, and the N-terminal signaling domain (NTSD) of PupB, an outer-membrane TonB-dependent transducer. The structure revealed that the CCSSD consists of two subdomains: a juxta-membrane subdomain, which has a novel all-β-fold, followed by a secretin/TonB, short N-terminal subdomain at the C terminus of the CCSSD, a previously unobserved topological arrangement of this domain. Using affinity pulldown assays, isothermal titration calorimetry, and thermal denaturation CD spectroscopy, we show that both subdomains are required for binding the NTSD with micromolar affinity and that NTSD binding improves CCSSD stability. Our findings prompt us to present a revised model of CSS wherein the CCSSD:NTSD complex forms prior to ferric-siderophore binding. Upon siderophore binding, conformational changes in the CCSSD enable regulated intramembrane proteolysis of the sigma regulator, ultimately resulting in transcriptional regulation.




surface

CBD News: Covering some 22 per cent of the world's land surface, mountains are home to spectacular landscapes, a wide variety of ecosystems, a great diversity of species, and distinctive human communities, with approximately 955 million people, or 13




surface

CBD News: Covering some 22 per cent of the world's land surface, mountains are home to spectacular landscapes, a wide variety of ecosystems, and a great diversity of species.