A flexible circuit board includes a first circuit substrate, a second circuit substrate and a bonding layer. The first circuit substrate includes a first base layer, a first circuit layer, a second circuit layer and metal coating layer. The first circuit layer includes a signal line and at least two grounding lines. The metal coating layer encloses the signal line. The second circuit substrate includes a third circuit layer. The bonding layer is located between and bonding the first circuit substrate and the second circuit substrate. The second circuit layer, the third circuit layer are electrically coupled with the grounding lines by a plurality of electrically conductive holes. The first base layer, the bonding layer and the second circuit substrate cooperatively enclose a hermetic medium layer receiving the signal line. The hermitic medium layer is filled with air. A method for manufacturing the flexible circuit board is also provided.

A flexible printed circuit board including an annular main board and a plurality of branches connected with the annular main board is provided. Each of the branches includes an extension portion connected with the annular main board and a bonding portion, and an electronic component is adapted to be disposed on the bonding portion. A supporting holder including an annular base, two wing structures, and a plurality of mounting portions is also disclosed, wherein the wing structures extend outward from the annular base and the mounting portions are located on the annular base and the wing structures. Further, a controller including the flexible printed circuit board and the supporting holder aforementioned is disclosed, wherein the annular main board is disposed on the annular base and the branches are disposed on the annular base and the wing structures, such that the bonding portions are located on the mounting portions correspondingly.

An apparatus and a system directed to an improved hydraulic circuit for use on implements powered by separate motorized vehicle such as a tractor. The apparatus and system include an implement, such as a bale processing or stacking device, configured with wheels to be a towed vehicle, with the towed vehicle configured to be conveyed by a tow vehicle. Additionally, a hydraulic pump on the tow vehicle is coupled to one or more accumulators on the towed device to provide hydraulic power at varying rates as needed by one or more mechanical operations of the implement vehicle. A hydraulic circuit including check and block valves in concert with a pressure switch on the implement device obtains hydraulic power from the tow vehicle from a hydraulic pump located on the tow vehicle via one or more hydraulic transfer lines from the tow vehicle to the implement.

An apparatus for separating and storing liquid refrigerant in a refrigerant circuit including a housing configured as a refrigerant collection container, the housing having a refrigerant outflow line disposed therein. The refrigerant outflow line extending from an inlet opening, which is disposed in a gas refrigerant region and above a level of the liquid refrigerant, via a liquid refrigerant region to the outside, and has a through-opening formed in the liquid refrigerant region. The housing includes a boiling element for liquid refrigerant disposed therein. The boiling element is connected to the refrigerant outflow line in the region of the through-opening, such that a liquid passing through the through-opening from the housing passes through the boiling element by suction of a gas refrigerant.

An ice making machine having a refrigeration system designed for hydrocarbon (HC) refrigerants, and particularly propane (R-290), that includes dual independent refrigeration systems and a unique evaporator assembly comprising of a single freeze plate attached to two cooling circuits. The serpentines are designed in an advantageous pattern that promotes efficiency by ensuring the even bridging of ice during freezing and minimizing unwanted melting during harvest by providing an even distribution of the heat load. The charge limitations imposed with flammable refrigerants would otherwise prevent large capacity ice maker from being properly charged with a single circuit. The ice making machine includes a single water circuit and control system to ensure the proper and efficient production of ice. Material cost is conserved as compared to a traditional dual system icemaker.

A system for adaptive cooling and energy harvesting comprising at least one thermoelectric device capable of acting as a thermoelectric cooler and as a thermoelectric generator, a hierarchical multiple-level control system, and electronics controlled by the control system and connected to the thermoelectric device. The electronics selectively configure the thermoelectric device in at least in a thermoelectric cooler operating mode and in a thermoelectric generation operating mode. The thermoelectric device can incorporate quantum-process and quantum-well materials for higher heat transfer and thermoelectric generation efficiencies. The invention provides for thermoelectric devices to additionally operate in temperature sensing mode. The hierarchical control system can comprise a plurality of control system, each of which can operate in isolation and can be interconnected with additional subsystems associated with other hierarchical levels. The hierarchical control system can comprise linear (additive) control, bilinear (additive and multiplicative) control, nonlinear control, and hysteresis.

The MIT Press, ISBN-13: 978-0262039802, English, 464 pages, 2019, USA

Nam June Paik is back in the limelight, thanks to an important historical exhibition at Tate Modern (see the report in this issue). There has →

Mike Pompeo is the last surviving member of President Trump’s original national-security team. Pompeo entered the Administration as the director of the C.I.A., but, after the sudden end of Rex Tillerson’s tenure as Secretary of State, Pompeo was elevated to the position of America’s top diplomat. All this despite the fact that Pompeo had no diplomatic experience, a résumé that includes exaggerations, and a history of criticizing Trump. Since the 2016 election, though, Pompeo has rebranded himself as a strong advocate for the President, and has come to embrace Trumpism alongside many other former critics in his party. Susan B. Glasser joins Eric Lach to discuss Pompeo’s journey from traditional California Republican to staunch Trump ally, and what it says about larger trends within the Republican Party.

One of the Top End's only female bull riders has taken on the US rodeo circuit despite suffering a serious shoulder injury just days from leaving.

The second stage of the ACT's light rail project will see a centrepiece of Canberra's city transformed, with the southern end of London Circuit raised in order to accommodate a tram line traversing down to Lake Burley Griffin.

A father who threatened to kill a Federal Circuit Court Judge in a late night, expletive-laden phone call walks free from a Far North Queensland court.

Incoming OSU Welding Engineering freshman Sam is getting excited for classes to start in a few weeks.  This summer, he’s been working two jobs, including an internship at OSU’s ElectroScience Lab. As part of the project I’m working on there, I obtained a radio assembly kit online and put it together in order to experience […]

Hear about Trekking the Annapurna Circuit in Nepal as the Amateur Traveler talks to the Su Family from sufamilyadventures.com about tackling this well known route.

Brian Zingg
Apr 15, 2020; 40:3250-3267
Systems/Circuits

Sebastian Onasch
Apr 15, 2020; 40:3186-3202
Systems/Circuits

M Chalfie
Apr 1, 1985; 5:956-964
Articles

Revealing the organization and function of neural circuits is greatly facilitated by viral tools that spread transsynaptically. Adeno-associated virus (AAV) exhibits anterograde transneuronal transport, however, the synaptic specificity of this spread and its broad application within a diverse set of circuits remains to be explored. Here, using anatomic, functional, and molecular approaches, we provide evidence for the preferential transport of AAV1 to postsynaptically connected neurons and reveal its spread is strongly dependent on synaptic transmitter release. In addition to glutamatergic pathways, AAV1 also spreads through GABAergic synapses to both excitatory and inhibitory cell types. We observed little or no transport, however, through neuromodulatory projections (e.g., serotonergic, cholinergic, and noradrenergic). In addition, we found that AAV1 can be transported through long-distance descending projections from various brain regions to effectively transduce spinal cord neurons. Combined with newly designed intersectional and sparse labeling strategies, AAV1 can be applied within a wide variety of pathways to categorize neurons according to their input sources, morphology, and molecular identities. These properties make AAV1 a promising anterograde transsynaptic tool for establishing a comprehensive cell-atlas of the brain, although its capacity for retrograde transport currently limits its use to unidirectional circuits.

SIGNIFICANCE STATEMENT The discovery of anterograde transneuronal spread of AAV1 generates great promise for its application as a unique tool for manipulating input-defined cell populations and mapping their outputs. However, several outstanding questions remain for anterograde transsynaptic approaches in the field: (1) whether AAV1 spreads exclusively or specifically to synaptically connected neurons, and (2) how broad its application could be in various types of neural circuits in the brain. This study provides several lines of evidence in terms of anatomy, functional innervation, and underlying mechanisms, to strongly support that AAV1 anterograde transneuronal spread is highly synapse specific. In addition, several potentially important applications of transsynaptic AAV1 in probing neural circuits are described.

Neurons and circuits each with a distinct balance of intrinsic and synaptic conductances can generate similar behavior but sometimes respond very differently to perturbation. Examining a large family of circuit models with non-identical neurons and synapses underlying rhythmic behavior, we analyzed the circuits' response to modifications in single and multiple intrinsic conductances in the individual neurons. To summarize these changes over the entire range of perturbed parameters, we quantified circuit output by defining a global stability measure. Using this measure, we identified specific subsets of conductances that when perturbed generate similar behavior in diverse individuals of the population. Our unbiased clustering analysis enabled us to quantify circuit stability when simultaneously perturbing multiple conductances as a nonlinear combination of single conductance perturbations. This revealed surprising conductance combinations that can predict the response to specific perturbations, even when the remaining intrinsic and synaptic conductances are unknown. Therefore, our approach can expose hidden variability in the balance of intrinsic and synaptic conductances of the same neurons across different versions of the same circuit solely from the circuit response to perturbations. Developed for a specific family of model circuits, our quantitative approach to characterizing high-dimensional degenerate systems provides a conceptual and analytic framework to guide future theoretical and experimental studies on degeneracy and robustness.

SIGNIFICANCE STATEMENT Neural circuits can generate nearly identical behavior despite neuronal and synaptic parameters varying several-fold between individual instantiations. Yet, when these parameters are perturbed through channel deletions and mutations or environmental disturbances, seemingly identical circuits can respond very differently. What distinguishes inconsequential perturbations that barely alter circuit behavior from disruptive perturbations that drastically disturb circuit output remains unclear. Focusing on a family of rhythmic circuits, we propose a computational approach to reveal hidden variability in the intrinsic and synaptic conductances in seemingly identical circuits based solely on circuit output to different perturbations. We uncover specific conductance combinations that work similarly to maintain stability and predict the effect of changing multiple conductances simultaneously, which often results from neuromodulation or injury.

We consider the question of how sensory networks enable the detection of sensory stimuli in a combinatorial coding space. We are specifically interested in the olfactory system, wherein recent experimental studies have reported the existence of rich, enigmatic response patterns associated with stimulus onset and offset. This study aims to identify the functional relevance of such response patterns (i.e., what benefits does such neural activity provide in the context of detecting stimuli in a natural environment). We study this problem through the lens of normative, optimization-based modeling. Here, we define the notion of a low-dimensional latent representation of stimulus identity, which is generated through action of the sensory network. The objective of our optimization framework is to ensure high-fidelity tracking of a nominal representation in this latent space in an energy-efficient manner. It turns out that the optimal motifs emerging from this framework possess morphologic similarity with prototypical onset and offset responses observed in vivo in locusts (Schistocerca americana) of either sex. Furthermore, this objective can be exactly achieved by a network with reciprocal excitatory–inhibitory competitive dynamics, similar to interactions between projection neurons and local neurons in the early olfactory system of insects. The derived model also makes several predictions regarding maintenance of robust latent representations in the presence of confounding background information and trade-offs between the energy of sensory activity and resultant behavioral measures such as speed and accuracy of stimulus detection.

SIGNIFICANCE STATEMENT A key area of study in olfactory coding involves understanding the transformation from high-dimensional sensory stimulus to low-dimensional decoded representation. Here, we examine not only the dimensionality reduction of this mapping but also its temporal dynamics, with specific focus on stimuli that are temporally continuous. Through optimization-based synthesis, we examine how sensory networks can track representations without prior assumption of discrete trial structure. We show that such tracking can be achieved by canonical network architectures and dynamics, and that the resulting responses resemble observations from neurons in the insect olfactory system. Thus, our results provide hypotheses regarding the functional role of olfactory circuit activity at both single neuronal and population scales.

MECP2 gain-of-function and loss-of-function in genetically engineered monkeys recapitulates typical phenotypes in patients with autism, yet where MECP2 mutation affects the monkey brain and whether/how it relates to autism pathology remain unknown. Here we report a combination of gene–circuit–behavior analyses including MECP2 coexpression network, locomotive and cognitive behaviors, and EEG and fMRI findings in 5 MECP2 overexpressed monkeys (Macaca fascicularis; 3 females) and 20 wild-type monkeys (Macaca fascicularis; 11 females). Whole-genome expression analysis revealed MECP2 coexpressed genes significantly enriched in GABA-related signaling pathways, whereby reduced β-synchronization within fronto-parieto-occipital networks was associated with abnormal locomotive behaviors. Meanwhile, MECP2-induced hyperconnectivity in prefrontal and cingulate networks accounted for regressive deficits in reversal learning tasks. Furthermore, we stratified a cohort of 49 patients with autism and 72 healthy controls of 1112 subjects using functional connectivity patterns, and identified dysconnectivity profiles similar to those in monkeys. By establishing a circuit-based construct link between genetically defined models and stratified patients, these results pave new avenues to deconstruct clinical heterogeneity and advance accurate diagnosis in psychiatric disorders.

SIGNIFICANCE STATEMENT Autism spectrum disorder (ASD) is a complex disorder with co-occurring symptoms caused by multiple genetic variations and brain circuit abnormalities. To dissect the gene–circuit–behavior causal chain underlying ASD, animal models are established by manipulating causative genes such as MECP2. However, it is unknown whether such models have captured any circuit-level pathology in ASD patients, as demonstrated by human brain imaging studies. Here, we use transgenic macaques to examine the causal effect of MECP2 overexpression on gene coexpression, brain circuits, and behaviors. For the first time, we demonstrate that the circuit abnormalities linked to MECP2 and autism-like traits in the monkeys can be mapped to a homogeneous ASD subgroup, thereby offering a new strategy to deconstruct clinical heterogeneity in ASD.

D. Brooks Smith, Chief Judge of the United States Court of Appeals for the Third Circuit, will deliver the Penn State Law in University Park 2020 commencement address on May 16 in Eisenhower Auditorium.

Prominent Indian-American lawyer Neomi Rao has been nominated by US President Donald Trump to fill Justice Brett Kavanaugh’s seat on the powerful DC Circuit Court of Appeals.

Presentation by Joe Fjelstad of Silicon Pipe, Inc.

Picka.treatsg, an online business operating out of a Singapore public flat, saw an increase in orders of its cakes late last month, after the authorities announced commercial snack and drinks outlets would have to temporarily stop operating.This came amid tighter restrictions during Singapore’s circuit breaker or partial lockdown period, and just before the start of the Muslim fasting month of Ramadan.Singapore moves nursing-home employees into hotels to protect elderlyWith orders growing from…

MPLAB PICkit4 In-Circuit Debugger User's Guide

MPLAB PICkit 4 In-Circuit Debugger Quick Start Guide

Title: Scientists Pinpoint Brain's 'Hunger Circuit' in Mice
Category: Health News
Created: 4/28/2015 12:00:00 AM
Last Editorial Review: 4/28/2015 12:00:00 AM

Although pain is a prevalent nonmotor symptom in Parkinson’s disease (PD), it is undertreated, in part because of our limited understanding of the underlying mechanisms. Considering that the basal ganglia are implicated in pain sensation, and that their synaptic outputs are controlled by the subthalamic nucleus (STN), we hypothesized that...

High-constitutive activity of the DNA damage response protein checkpoint kinase 1 (CHK1) has been shown in glioblastoma (GBM) cell lines and in tissue sections. However, whether constitutive activation and overexpression of CHK1 in GBM plays a functional role in tumorigenesis or has prognostic significance is not known. We interrogated multiple glioma patient cohorts for expression levels of CHK1 and the oncogene cancerous inhibitor of protein phosphatase 2A (CIP2A), a known target of high-CHK1 activity, and examined the relationship between these two proteins in GBM. Expression levels of CHK1 and CIP2A were independent predictors for reduced overall survival across multiple glioma patient cohorts. Using siRNA and pharmacologic inhibitors we evaluated the impact of their depletion using both in vitro and in vivo models and sought a mechanistic explanation for high CIP2A in the presence of high-CHK1 levels in GBM and show that; (i) CHK1 and pSTAT3 positively regulate CIP2A gene expression; (ii) pSTAT3 and CIP2A form a recursively wired transcriptional circuit; and (iii) perturbing CIP2A expression induces GBM cell senescence and retards tumor growth in vitro and in vivo. Taken together, we have identified an oncogenic transcriptional circuit in GBM that can be destabilized by targeting CIP2A.

Peripheral somatosensory input is modulated in the dorsal spinal cord by a network of excitatory and inhibitory interneurons. PTF1A is a transcription factor essential in dorsal neural tube progenitors for specification of these inhibitory neurons. Thus, mechanisms regulating Ptf1a expression are key for generating neuronal circuits underlying somatosensory behaviors. Mutations targeted to distinct cis-regulatory elements for Ptf1a in mice, tested the in vivo contribution of each element individually and in combination. Mutations in an autoregulatory enhancer resulted in reduced levels of PTF1A, and reduced numbers of specific dorsal spinal cord inhibitory neurons, particularly those expressing Pdyn and Gal. Although these mutants survive postnatally, at ~3–5 wk they elicit a severe scratching phenotype. Behaviorally, the mutants have increased sensitivity to itch, but acute sensitivity to other sensory stimuli such as mechanical or thermal pain is unaffected. We demonstrate a requirement for positive transcriptional autoregulatory feedback to attain the level of the neuronal specification factor PTF1A necessary for generating correctly balanced neuronal circuits.

Get ready for the first scored event in DARPA's latest Grand Challenge

It's about more than just first place as teams look toward more competitions in 2020

Thanks to its unique three-dimensional manufacturing capacity, ultrafast laser writing is a prime candidate to meet the growing demand for the miniaturization of photonic circuitry, e.g., for scaling up optical quantum computers capacity. Towards this goal, scientists from Canada discovered a phenomenon related to the material electronic resonance that allows a much greater miniaturization of the laser written devices. Surprisingly, the new phenomenon allows other intriguing applications such as a new concept of invisibility.

On May 15, 2009, the Fifth Circuit, in Klamath Strategic Investment Fund v. United States (No. 07-40861), affirmed the district court’s decision denying over $50 million in claimed tax losses arising from the taxpayers’ investment in a “Son of Boss (BLIPS)” tax shelter.

"Our system is predicated on an adversarial model and the separation of powers between the branches but that separation need not make us adversaries or antagonists. Rather, it makes us essential partners in balancing that tension to ensure our system protects the fundamental principles of liberty, equal justice, and the rule of law."

"In addition to providing an update on some of the Justice Department’s top priorities, I’d also like to discuss the work we’re doing – and the historic commitment we’ve made – to ensure that justice is done in each and every criminal case our federal prosecutors pursue," said Attorney General Holder.

"As I have seen every day at the Department of Justice – in national-security briefings, reviews of case files, and meetings with the leaders of our divisions and task forces – our role as lawyers has evolved," said Acting Deputy Attorney General Grindler.

Peter Picone, 41, of Methuen, Massachusetts, pleaded guilty today in U.S. District Court in Hartford, Connecticut to importing thousands of counterfeit integrated circuits (ICs) from China and Hong Kong and then reselling them to U.S. customers.

Pharmaceutical and biotech companies breathed a sigh of relief Monday when the Federal Circuit unanimously ruled in a precedential opinion that the mere sale of manufacturing services to create embodiments of a patented product is not a “commercial sale” of the invention that triggers the on-sale bar of 35 U.S.C. § 102(b) (pre-AIA).[1]  The en banc opinion...… Continue Reading

McLaren team boss Martin Whitmarsh has hit out at the design of the Abu Dhabi circuit, saying that the modern Yas Marina venue is flawed