Tech giants including Meta, Snap, Google and others are racing to build smart glasses. It could change our lives for the better or worse.

Peace on Earth (archive) - "After mankind is wiped out in the final war, forest animals re-build for a better tomorrow."

• The Cuddliest Post-Apocalyptic Tale Ever - "And that prompts the squirrel children to ask: 'What are men, Grandpa?'"
• Peace On Earth was nominated for an Oscar - "It features an uncredited Mel Blanc as a wise Grandpa squirrel, who helps build what he calls 'a great old world' inspired by the Bible and anchored in nonviolence."

Steve Beauchampé takes a trip.

Manos Nikolakis, GM, BIC Cello, said, "While e-commerce is growing significantly across the country, the landscape of the Indian market is still heavily reliant on traditional trade. Accordingly, this remains our main sales driver. The lack of digital penetration in semi-urban and rural areas can sometimes create a challenge in the e-commerce space, which means that e-commerce is mostly relevant in bigger cities with a more mature infrastructure."

Driven by central bank purchases and geopolitical tensions, gold prices soared to 39 new all-time highs in 2024, marking the highest number in 45 years. The surge, influenced by strong Asian demand and US election anxieties, saw gold reach $2,734 per ounce, prompting predictions of further increases.

From cookbooks to constitutional rights, On Second Thought is proud to present another five stories from our archive to motivate you this Monday. 1) Historian Jill Lepore Explores 'These Truths' Of United States History In November 2018, On Second Thought sat down with Harvard American history professor Jill Lepore to discuss her book These Truths: A History of the United States and the obligation to learn from the past for a brighter future. Focusing on promises made in the Constitution, Lepore discusses the state of institutions like freedom, voting, and social struggles almost 250 years after the country’s founding. 2) Chef Pano Karatassos On 'Modern Greek Cooking' Atlanta chef Pano Karatassos made waves in culinary circles after winning Food Network’s Beat Bobby Flay with his signature lamb pie. Chef Karatassos is the executive chef of Kyma in Atlanta and has tasked himself with bringing traditional Greek foods to the South. He sat down with us last October to talk Greek cuisine

The first angel of Revelation 14 proclaims a judgment that occurs before Jesus comes in glory. Once every case has been decided in the heavenly courtroom, Jesus will return with His rewards.

The first angel of Revelation 14 proclaims a judgment that occurs before Jesus comes in glory. Once every case has been decided in the heavenly courtroom, Jesus will return with His rewards.

NDP leader Jagmeet Singh tells CBC Radio's The House that he’s not looking to force an election this fall if the Liberal government follows through on commitments to help women and other marginalized groups affected by the COVID-19 lockdown.

We have a friend from Flipora, which we did not know we had... Oh sorry our mistake, iyaloo27@gmail.com is not our friend, he/she is a spammer and spammers are our enemy.

In casestudies in de literatuur of uit het werkveld lezen we vaak over de grote meerwaarde die neuromarketing biedt aan marketinggerichte projecten.  Maar wat is de realiteit? Wat zijn de effectieve mogelijkheden? Wat is het verschil tussen de verwachte potentie van neuromarketing en de daadwerkelijke mogelijkheden, uitdagingen en beperkingen ervan in de praktijk. En, wat […]

But the most dangerous attackers of American democracy aren’t the Russians or the Chinese. They are the leaders of the Republican Party.

In This Episode Erin and Weer’d discuss: a declaration that suspended the 2nd Amendment in a Florida city during Hurricane Helene; a police chief in Massachusetts who was reprimanded for speaking out against an unconstitutional law; Brandon Herrera’s re-creation of … Continue reading →

Social Care Institute for Excellence (SCIE) Report 71 from SCIE Roundtable held on 12 February 2015. The aim of the Social Care Institute for Excellence (SCIE) roundtable discussion was to identify, celebrate, support and learn from community-led activity.

New on NextDoorStudios, an ASGMax site: Colt Spence would do anything to just relax today. He’s been beyond stressed out from work and his wife won’t stop calling him incessantly just to check in and see how he’s doing. Finally thinking he has some alone time, he ends up getting a surprise visitor when Andrew... View Article

The post ASGMax | NextDoorStudios: Andrew Delta and Colt Spence – “No Time to Relax” appeared first on QueerClick.

“Chigumadzi argues that within the liberal international order, it is “reasonable” and “workable” to struggle to end apartheid and racial segregation, while it is “unreasonable” and “unworkable” to struggle to end settler colonialism and indigenous land dispossession. In arguing that apartheid is overrepresented in the International Left’s racial discourse and historiography, Chigumadzi draws from generations of Black South African political activists, philosophers, and historians—most notably from the Pan Africanist-Black Consciousness Tradition. These traditions critique apartheid’s relatively short 54 years of institutionalized racial segregation as the paradigmatic historical framework for analyzing South Africa’s three centuries of settler colonialism and land dispossession. Drawing from this black radical critique, Chigumadzi rejects the liberal notion that apartheid’s end is the object of liberation struggle, and, instead asserts the centrality of the struggle for the return of indigenous lands.” Dr. Panashe Chigumadzi is an award-winning writer and Assistant Professor of African History at Brandeis University. Chigumadzi holds a doctorate from Harvard University’s Department of African and African American Studies, and a masters in African Literature from the University of the Witwatersrand, Johannesburg, South Africa.

The project is from Mattel Films, Shadowmachine, Jennifer Lopez’s Nuyorican Productions, and actor-producer Anthony Ramos.

Time to cross the border to Johor Bahru for a shopping and dining experience. To start this ultimate shopping experience, head to one of the closest malls to Singapore, Paradigm Mall JB, located in Johor Bahru. Paradigm Mall Johor Bahru is also known as the largest shopping mall in Johor Bahru. There are more than ... Read more

The post Top 4 Must-Try Desserts In Paradigm Mall JB (Malaysia) appeared first on Star Two.

Described by some as an "enigma", Mrs Trump is expected to return to her White House duties "on her own terms".

Nuclear proliferation in a fragmenting world 13 September 2022 — 1:00PM TO 2:00PM Anonymous (not verified) 5 September 2022 Online

Where does the Treaty on the Non-Proliferation of Nuclear Weapons (NPT) stand in 2022?

With the war in Ukraine and China–US tensions rising, the threat of a nuclear arms race has never been as high since the Cold War.

Global efforts to control the appropriation and use of nuclear weapons are strained with the war in Ukraine, US–Russia and US–China tension, Iran on the nuclear threshold, instability in Pakistan and the ongoing threat from North Korea.

The Review Conference of the NPT that took place in New York this August sought to deal with the multiple nuclear threats, but new missile technology such as hypersonic glide vehicles and torpedoes are not covered by a treaty.

Russia and the US are in a development phase for a host of new technologies and delivery systems, especially after both withdrew from various arms-control treaties in the 2000s and 2010s. Now the nuclear stockpile is growing again and major superpowers are adopting confrontational geostrategic positions.

As the world’s established orders and system fragment, there is a possibility the nine countries with confirmed nuclear weapons could grow. Events in Ukraine highlight the need for a new, modern agreement on nuclear weapons control, needed today more than ever.

The panel of experts discuss key questions including:

• What did ‘RevCon’ in New York tell us about the adoption or update to the NPT?

• Will the war in Ukraine deter or prevent any agreement between nations or does nuclear weapons control represent an area of potential multilateral harmony?

• Are we likely to see a new arms race akin to the Cold War? Where does China fit into this race?

• How has technology changed the arms control landscape?

 As with all Chatham House member events, questions from members drive the conversation.

A. V. Domrina
Trans. Moscow Math. Soc. 83 (), 201-215.
Abstract, references and article information

Martin Ehler and Karlheinz Gröchenig
Math. Comp. 93 (), 2885-2919.
Abstract, references and article information

The bacterial enhancer-binding protein (bEBP) FlrC, controls motility and colonization of Vibrio cholerae by regulating the transcription of class-III flagellar genes in σ54-dependent manner. However, the mechanism by which FlrC regulates transcription is not fully elucidated. Although, most bEBPs require nucleotides to stimulate the oligomerization necessary for function, our previous study showed that the central domain of FlrC (FlrCC) forms heptamer in a nucleotide-independent manner. Furthermore, heptameric FlrCC binds ATP in “cis-mediated” style without any contribution from sensor I motif 285REDXXYR291 of the trans protomer. This atypical ATP binding raises the question of whether heptamerization of FlrC is solely required for transcription regulation, or if it is also critical for ATPase activity. ATPase assays and size exclusion chromatography of the trans-variants FlrCC-Y290A and FlrCC-R291A showed destabilization of heptameric assembly with concomitant abrogation of ATPase activity. Crystal structures showed that in the cis-variant FlrCC-R349A drastic shift of Walker A encroached ATP-binding site, whereas the site remained occupied by ADP in FlrCC-Y290A. We postulated that FlrCC heptamerizes through concentration-dependent cooperativity for maximal ATPase activity and upon heptamerization, packing of trans-acting Tyr290 against cis-acting Arg349 compels Arg349 to maintain proper conformation of Walker A. Finally, a Trp quenching study revealed binding of cyclic-di-GMP with FlrCC. Excess cyclic-di-GMP repressed ATPase activity of FlrCC through destabilization of heptameric assembly, especially at low concentration of protein. Systematic phylogenetic analysis allowed us to propose similar regulatory mechanisms for FlrCs of several Vibrio species and a set of monotrichous Gram-negative bacteria.

Peptidoglycan (PG) is an essential constituent of the bacterial cell wall. During cell division, the machinery responsible for PG synthesis localizes mid-cell, at the septum, under the control of a multiprotein complex called the divisome. In Escherichia coli, septal PG synthesis and cell constriction rely on the accumulation of FtsN at the division site. Interestingly, a short sequence of FtsN (Leu75–Gln93, known as EFtsN) was shown to be essential and sufficient for its functioning in vivo, but what exactly this sequence is doing remained unknown. Here, we show that EFtsN binds specifically to the major PG synthase PBP1b and is sufficient to stimulate its biosynthetic glycosyltransferase (GTase) activity. We also report the crystal structure of PBP1b in complex with EFtsN, which demonstrates that EFtsN binds at the junction between the GTase and UB2H domains of PBP1b. Interestingly, mutations to two residues (R141A/R397A) within the EFtsN-binding pocket reduced the activation of PBP1b by FtsN but not by the lipoprotein LpoB. This mutant was unable to rescue the ΔponB-ponAts strain, which lacks PBP1b and has a thermosensitive PBP1a, at nonpermissive temperature and induced a mild cell-chaining phenotype and cell lysis. Altogether, the results show that EFtsN interacts with PBP1b and that this interaction plays a role in the activation of its GTase activity by FtsN, which may contribute to the overall septal PG synthesis and regulation during cell division.

Genetic mutations related to ALS, a progressive neurological disease, have been discovered in the gene encoding σ-1 receptor (σ1R). We previously reported that σ1RE102Q elicits toxicity in cells. The σ1R forms oligomeric states that are regulated by ligands. Nevertheless, little is known about the effect of ALS-related mutations on oligomer formation. Here, we transfected NSC-34 cells, a motor neuronal cell line, and HEK293T cells with σ1R-mCherry (mCh), σ1RE102Q-mCh, or nontagged forms to investigate detergent solubility and subcellular distribution using immunocytochemistry and fluorescence recovery after photobleaching. The oligomeric state was determined using crosslinking procedure. σ1Rs were soluble to detergents, whereas the mutants accumulated in the insoluble fraction. Within the soluble fraction, peak distribution of mutants appeared in higher sucrose density fractions. Mutants formed intracellular aggregates that were co-stained with p62, ubiquitin, and phosphorylated pancreatic eukaryotic translation initiation factor-2-α kinase in NSC-34 cells but not in HEK293T cells. The aggregates had significantly lower recovery in fluorescence recovery after photobleaching. Acute treatment with σ1R agonist SA4503 failed to improve recovery, whereas prolonged treatment for 48 h significantly decreased σ1RE102Q-mCh insolubility and inhibited apoptosis. Whereas σ1R-mCh formed monomers and dimers, σ1RE102Q-mCh also formed trimers and tetramers. SA4503 reduced accumulation of the four types in the insoluble fraction and increased monomers in the soluble fraction. The σ1RE102Q insolubility was diminished by σ1R-mCh co-expression. These results suggest that the agonist and WT σ1R modify the detergent insolubility, toxicity, and oligomeric state of σ1RE102Q, which may lead to promising new treatments for σ1R-related ALS.

Amyloid fibrils are polymeric structures originating from aggregation of misfolded proteins. In vivo, proteolysis may modulate amyloidogenesis and fibril stability. In light chain (AL) amyloidosis, fragmented light chains (LCs) are abundant components of amyloid deposits; however, site and timing of proteolysis are debated. Identification of the N and C termini of LC fragments is instrumental to understanding involved processes and enzymes. We investigated the N and C terminome of the LC proteoforms in fibrils extracted from the hearts of two AL cardiomyopathy patients, using a proteomic approach based on derivatization of N- and C-terminal residues, followed by mapping of fragmentation sites on the structures of native and fibrillar relevant LCs. We provide the first high-specificity map of proteolytic cleavages in natural AL amyloid. Proteolysis occurs both on the LC variable and constant domains, generating a complex fragmentation pattern. The structural analysis indicates extensive remodeling by multiple proteases, largely taking place on poorly folded regions of the fibril surfaces. This study adds novel important knowledge on amyloid LC processing: although our data do not exclude that proteolysis of native LC dimers may destabilize their structure and favor fibril formation, the data show that LC deposition largely precedes the proteolytic events documentable in mature AL fibrils.

You might receive a Segmentation Violation when opening a database table in SAS. The SAS Log contains the error and traceback:

Since the publication of the Age-Related Eye Disease Study (AREDS2) in 2013, the macular pigment carotenoids lutein and zeaxanthin have become well known to both the eye care community and the public. It is a fascinating aspect of evolution that primates have repurposed photoprotective pigments and binding proteins from plants and insects to protect and enhance visual acuity. Moreover, utilization of these plant-derived nutrients has been widely embraced for preventing vision loss from age-related macular degeneration (AMD). More recently, there has been growing awareness that these nutrients can also play a role in improving visual performance in adults. On the other hand, the potential benefits of lutein and zeaxanthin supplementation at very young ages have been underappreciated. In this review, we examine the biochemical mechanisms and supportive data for lutein and zeaxanthin supplementation throughout the lifespan, with particular emphasis on prenatal supplementation. We propose that prenatal nutritional recommendations may aim at improving maternal and infant carotenoid status. Prenatal supplementation with lutein and zeaxanthin might enhance infant visual development and performance and may even prevent retinopathy of prematurity, possibilities that should be examined in future clinical studies.

The essential fatty acid DHA (22:6, omega-3 or n-3) is enriched in and required for the membrane biogenesis and function of photoreceptor cells (PRC), synapses, mitochondria, etc. of the CNS. PRC DHA becomes an acyl chain at the sn-2 of phosphatidylcholine (PC), amounting to more than 50% of the PRC outer segment phospholipids, where phototransduction takes place. Very long chain PUFAs (VLC-PUFAs,n-3, ≥ 28 carbons) are at the sn-1 of this PC molecular species and interact with rhodopsin. PRC shed their tips (DHA-rich membrane disks) daily, which in turn are phagocytized by the retinal pigment epithelium (RPE), where DHA is recycled back to PRC inner segments to be used for the biogenesis of new photoreceptor membranes. Here, we review the structures and stereochemistry of novel elovanoid (ELV)-N32 and ELV-N34 to be ELV-N32: (14Z,17Z,20R,21E,23E,25Z,27S,29Z)-20,27-dihydroxydo-triaconta-14,17,21,23,25,29-hexaenoic acid; ELV-N34: (16Z,19Z,22R,23E,25E,27Z,29S,31Z)-22,29-dihydroxytetra-triaconta-16,19,23,25,27,31-hexaenoic acid. ELVs are low-abundance, high-potency, protective mediators. Their bioactivity includes enhancing of anti-apoptotic and pro-survival protein expression with concomitant downregulation of pro-apoptotic proteins when RPE is confronted with uncompensated oxidative stress (UOS). ELVs also target PRC/RPE senescence gene programming, the senescence secretory phenotype in the interphotoreceptor matrix (IPM), as well as inflammaging (chronic, sterile, low-grade inflammation). An important lesson on neuroprotection is highlighted by the ELV mediators that target the terminally differentiated PRC and RPE, sustaining a beautifully synchronized renewal process. The role of ELVs in PRC and RPE viability and function uncovers insights on disease mechanisms and the development of therapeutics for age-related macular degeneration (AMD), Alzheimer’s disease (AD), and other pathologies.

Glycosylation is a prevalent, yet heterogeneous modification with a broad range of implications in molecular biology. This heterogeneity precludes enrichment strategies that can be universally beneficial for all glycan classes. Thus, choice of enrichment strategy has profound implications on experimental outcomes. Here we review common enrichment strategies used in modern mass spectrometry (MS)-based glycoproteomic experiments, including lectins and other affinity chromatographies, hydrophilic interaction chromatography (HILIC) and its derivatives, porous graphitic carbon (PGC), reversible and irreversible chemical coupling strategies, and chemical biology tools that often leverage bioorthogonal handles. Interest in glycoproteomics continues to surge as MS instrumentation and software improve, so this review aims to help equip researchers with necessary information to choose appropriate enrichment strategies that best complement these efforts.

Researchers have a clearer picture than ever before of how common conditions that involve aggressive behavior emerge and how treatment can help

WHAT MAKES JDM DISTINCT By Dan Goldstein As you navigate the academic world, you will inevitably have an exchange in which people ask you what field you are in. You will reply that you do JDM and people will ask you what JDM means. You will say “judgment and decision making” and then they will […]

The former Edmonton Oilers general manager has a new gig.

Longtime Red Wings GM and Hockey Hall of Famer Ken Holland joined the NHL in a new consulting role just months after parting with the Edmonton Oilers.

Former Red Wings general manager Ken Holland is taking on a new role with the NHL's league office

Poile, Nashville's first-ever general manager who retired in 2023, was inducted into the Hockey Hall of Fame alongside former Predators captain Shea Weber.

Vertebrate nervous systems use the axon initial segment (AIS) to initiate action potentials and maintain neuronal polarity. The microtubule-associated protein tripartite motif containing 46 (TRIM46) was reported to regulate axon specification, AIS assembly, and neuronal polarity through the bundling, or fasciculation, of microtubules in the proximal axon. However, these claims are based on TRIM46 knockdown in cultured neurons. To investigate TRIM46 function in vivo, we examined male and female TRIM46 knock-out mice. Contrary to previous reports, we find that TRIM46 is dispensable for axon specification and AIS formation. TRIM46 knock-out mice are viable, have normal behavior, and have normal brain structure. Thus, TRIM46 is not required for AIS formation, axon specification, or nervous system function. However, we confirm that TRIM46 is required for microtubule fasciculation. We also show TRIM46 enrichment in the first ~100 μm of axon occurs independently of ankyrinG (AnkG) in vivo, although AnkG is required to restrict TRIM46 only to the AIS. Our results highlight the need for further investigation of the mechanisms by which the AIS and microtubules interact to shape neuronal structure and function.

Injuries to the central nervous system (CNS) can cause severe neurological deficits. Axonal regrowth is a fundamental process for the reconstruction of compensatory neuronal networks after injury; however, it is extremely limited in the adult mammalian CNS. In this study, we conducted a loss-of-function genetic screen in cortical neurons, combined with a Web resource-based phenotypic screen, and identified synaptotagmin 4 (Syt4) as a novel regulator of axon elongation. Silencing Syt4 in primary cultured cortical neurons inhibits neurite elongation, with changes in gene expression involved in signaling pathways related to neuronal development. In a spinal cord injury model, inhibition of Syt4 expression in cortical neurons prevented axonal sprouting of the corticospinal tract, as well as neurological recovery after injury. These results provide a novel therapeutic approach to CNS injury by modulating Syt4 function.

Policy makers should support a broad array of approaches to overhauling global food systems, [...]

The Food and Agriculture Organization of the United Nations (FAO), in collaboration with the Secretariat of the Convention on Biological Diversity (CBD), will hold a Global Dialogue on the [...]