Over the past decade, modern methods of MS (MS) have emerged that allow reliable, fast and cost-effective identification of pathogenic microorganisms. Although MALDI-TOF MS has already revolutionized the way microorganisms are identified, recent years have witnessed also substantial progress in the development of liquid chromatography (LC)-MS based proteomics for microbiological applications. For example, LC-tandem MS (LC-MS²) has been proposed for microbial characterization by means of multiple discriminative peptides that enable identification at the species, or sometimes at the strain level. However, such investigations can be laborious and time-consuming, especially if the experimental LC-MS² data are tested against sequence databases covering a broad panel of different microbiological taxa. In this proof of concept study, we present an alternative bottom-up proteomics method for microbial identification. The proposed approach involves efficient extraction of proteins from cultivated microbial cells, digestion by trypsin and LC–MS measurements. Peptide masses are then extracted from MS¹ data and systematically tested against an in silico library of all possible peptide mass data compiled in-house. The library has been computed from the UniProt Knowledgebase covering Swiss-Prot and TrEMBL databases and comprises more than 12,000 strain-specific in silico profiles, each containing tens of thousands of peptide mass entries. Identification analysis involves computation of score values derived from correlation coefficients between experimental and strain-specific in silico peptide mass profiles and compilation of score ranking lists. The taxonomic positions of the microbial samples are then determined by using the best-matching database entries. The suggested method is computationally efficient – less than 2 mins per sample - and has been successfully tested by a test set of 39 LC-MS¹ peak lists obtained from 19 different microbial pathogens. The proposed method is rapid, simple and automatable and we foresee wide application potential for future microbiological applications.

Endometrial carcinoma (EC) is the most common gynecologic malignancy in the United States, with limited effective targeted therapies. Endometrial tumors exhibit frequent alterations in protein kinases, yet only a small fraction of the kinome has been therapeutically explored. To identify kinase therapeutic avenues for EC, we profiled the kinome of endometrial tumors and normal endometrial tissues using Multiplexed Inhibitor Beads and Mass Spectrometry (MIB-MS). Our proteomics analysis identified a network of kinases overexpressed in tumors, including Serine/Arginine-Rich Splicing Factor Kinase 1 (SRPK1). Immunohistochemical (IHC) analysis of endometrial tumors confirmed MIB-MS findings and showed SRPK1 protein levels were highly expressed in endometrioid and uterine serous cancer (USC) histological subtypes. Moreover, querying large-scale genomics studies of EC tumors revealed high expression of SRPK1 correlated with poor survival. Loss-of-function studies targeting SRPK1 in an established USC cell line demonstrated SRPK1 was integral for RNA splicing, as well as cell cycle progression and survival under nutrient deficient conditions. Profiling of USC cells identified a compensatory response to SRPK1 inhibition that involved EGFR and the up-regulation of IGF1R and downstream AKT signaling. Co-targeting SRPK1 and EGFR or IGF1R synergistically enhanced growth inhibition in serous and endometrioid cell lines, representing a promising combination therapy for EC.

AAA+ ATPases constitute a large family of proteins that are involved in a plethora of cellular processes including DNA disassembly, protein degradation and protein complex disassembly. They typically form a hexametric ring-shaped structure with six subunits in a (pseudo) 6-fold symmetry. In a subset of AAA+ ATPases that facilitate protein unfolding and degradation, six subunits cooperate to translocate protein substrates through a central pore in the ring. The number and type of nucleotides in an AAA+ ATPase hexamer is inherently linked to the mechanism that underlies cooperation among subunits and couples ATP hydrolysis with substrate translocation. We conducted a native MS study of a monodispersed form of PAN, an archaeal proteasome AAA+ ATPase, to determine the number of nucleotides bound to each hexamer of the WT protein. We utilized ADP and its analogs (TNP-ADP and mant-ADP), and a nonhydrolyzable ATP analog (AMP-PNP) to study nucleotide site occupancy within the PAN hexamer in ADP- and ATP-binding states, respectively. Throughout all experiments we used a Walker A mutant (PAN^K217A) that is impaired in nucleotide binding as an internal standard to mitigate the effects of residual solvation on mass measurement accuracy and to serve as a reference protein to control for nonspecific nucleotide binding. This approach led to the unambiguous finding that a WT PAN hexamer carried – from expression host – six tightly bound ADP molecules that could be exchanged for ADP and ATP analogs. Although the Walker A mutant did not bind ADP analogs, it did bind AMP-PNP, albeit at multiple stoichiometries. We observed variable levels of hexamer dissociation and an appearance of multimeric species with the over-charged molecular ion distributions across repeated experiments. We posit that these phenomena originated during ESI process at the final stages of ESI droplet evolution.

This article provides an introduction to Fourier transform-based mass spectrometry (FTMS). The key performance characteristics of FTMS, mass accuracy and resolution, are presented in the view of how they impact the interpretation of measurements in proteomic applications. The theory and principles of operation of two types of mass analyzer, Fourier transform ion cyclotron resonance and Orbitrap, are described. Major benefits as well as limitations of FTMS technology are discussed in the context of practical sample analysis, and illustrated with examples included as figures in this text and in the accompanying slide set. Comparisons highlighting the performance differences between the two mass analyzers are made where deemed useful in assisting the user with choosing the most appropriate technology for his/her application. Recent developments of these high-performing mass spectrometers are mentioned to provide a future outlook.

This article has been withdrawn by the authors. We discovered an error after this manuscript was published as a Paper in Press. Specifically, we learned that the structures of glycans presented for the PD-L1 peptide were drawn and labeled incorrectly. We wish to withdraw this article and submit a corrected version for review.

Complex protein glycosylation occurs through biosynthetic steps in the secretory pathway that create macro- and microheterogeneity of structure and function.  Required for all life forms, glycosylation diversifies and adapts protein interactions with binding partners that underpin interactions at cell surfaces and pericellular and extracellular environments. Because these biological effects arise from heterogeneity of structure and function, it is necessary to measure their changes as part of the quest to understand nature.  Quite often, however, the assumption behind proteomics that post-translational modifications are discrete additions that can be modeled using the genome as a template does not apply to protein glycosylation.  Rather, it is necessary to quantify the glycosylation distribution at each glycosite and to aggregate this information into a population of mature glycoproteins that exist in a given biological system.  To date, mass spectrometric methods for assigning singly glycosylated peptides are well-established.  But it is necessary to quantify glycosylation heterogeneity accurately in order to gauge the alterations that occur during biological processes.  The task is to quantify the glycosylated peptide forms as accurately as possible and then apply appropriate bioinformatics algorithms to the calculation of micro- and macro-similarities.  In this review, we summarize current approaches for protein quantification as they apply to this glycoprotein similarity problem.

This review covers recent developments in glycosaminoglycan (GAG) analysis via mass spectrometry (MS). GAGs participate in a variety of biological functions, including cellular communication, wound healing, and anticoagulation, and are important targets for structural characterization. GAGs exhibit a diverse range of structural features due to the variety of O- and N-sulfation modifications and uronic acid C-5 epimerization that can occur, making their analysis a challenging target. Mass spectrometry approaches to the structure assignment of GAGs have been widely investigated, and new methodologies remain the subject of development. Advances in sample preparation, tandem MS techniques (MS/MS), on-line separations and automated analysis software have advanced the field of GAG analysis. These recent developments have led to remarkable improvements in the precision and time efficiency for the structural characterization of GAGs.

Glycosylation is a prevalent, yet heterogeneous modification with a broad range of implications in molecular biology. This heterogeneity precludes enrichment strategies that can be universally beneficial for all glycan classes. Thus, choice of enrichment strategy has profound implications on experimental outcomes. Here we review common enrichment strategies used in modern mass spectrometry (MS)-based glycoproteomic experiments, including lectins and other affinity chromatographies, hydrophilic interaction chromatography (HILIC) and its derivatives, porous graphitic carbon (PGC), reversible and irreversible chemical coupling strategies, and chemical biology tools that often leverage bioorthogonal handles. Interest in glycoproteomics continues to surge as MS instrumentation and software improve, so this review aims to help equip researchers with necessary information to choose appropriate enrichment strategies that best complement these efforts.

Histone post-translational modifications (PTMs) are one of the main mechanisms of epigenetic regulation. Dysregulation of histone PTMs leads to many human diseases, such as cancer. Due to its high-throughput, accuracy, and flexibility, mass spectrometry (MS) has emerged as a powerful tool in the epigenetic histone modification field, allowing the comprehensive and unbiased analysis of histone PTMs and chromatin-associated factors. Coupled with various techniques from molecular biology, biochemistry, chemical biology and biophysics, MS has been employed to characterize distinct aspects of histone PTMs in the epigenetic regulation of chromatin functions. In this review we will describe advancements in the field of MS that have facilitated the analysis of histone PTMs and chromatin biology.  

The early detection of pancreatic ductal adenocarcinoma is a complex clinical obstacle yet is key to improving the overall likelihood of patient survival. Current and prospective carbohydrate biomarkers CA19-9 and sTRA are sufficient for surveilling disease progression yet are not approved for delineating PDAC from other abdominal cancers and non-cancerous pancreatic pathologies. To further understand these glycan epitopes, an imaging mass spectrometry approach was utilized to assess the N-glycome of the human pancreas and pancreatic cancer in a cohort of PDAC patients represented by tissue microarrays and whole tissue sections. Orthogonally, these same tissues were characterized by multi-round immunofluorescence which defined expression of CA19-9 and sTRA as well as other lectins towards carbohydrate epitopes with the potential to improve PDAC diagnosis. These analyses revealed distinct differences not only in N-glycan spatial localization across both healthy and diseased tissues but importantly between different biomarker-categorized tissue samples. Unique sulfated bi-antennary N-glycans were detected specifically in normal pancreatic islets. N-glycans from CA19-9 expressing tissues tended to be bi-, tri- and tetra-antennary structures with both core and terminal fucose residues and bisecting N-acetylglucosamines. These N-glycans were detected in less abundance in sTRA-expressing tumor tissues, which favored tri- and tetra-antennary structures with polylactosamine extensions. Increased sialylation of N-glycans was detected in all tumor tissues. A candidate new biomarker derived from IMS was further explored by fluorescence staining with selected lectins on the same tissues. The lectins confirmed the expression of the epitopes in cancer cells and revealed different tumor-associated staining patterns between glycans with bisecting GlcNAc and those with terminal GlcNAc. Thus, the combination of lectin-IHC and IMS techniques produces more complete information for tumor classification than the individual analyses alone. These findings potentiate the development of early assessment technologies to rapidly and specifically identify PDAC in the clinic that may directly impact patient outcomes.

Urinary proteomics studies have primarily focused on identifying markers of chronic kidney disease (CKD) progression. Here, we aimed to determine urinary markers of CKD renal parenchymal injury through proteomics analysis in animal kidney tissues and cells and in the urine of patients with CKD. Label-free quantitative proteomics analysis based on liquid chromatography-tandem mass spectrometry was performed on urine samples obtained from 6 normal controls and 9, 11, and 10 patients with CKD stages 1, 3, and 5, respectively, and on kidney tissue samples from a rat CKD model by 5/6 nephrectomy. Tandem mass tag-based quantitative proteomics analysis was performed for primary cultured glomerular endothelial cells (GECs) and proximal tubular epithelial cells (PTECs) before and after inducing 24-h hypoxia injury. Upon hierarchical clustering, out of 858 differentially expressed proteins (DEPs) in the urine of CKD patients, the levels of 416 decreased and 403 increased sequentially according to the disease stage, respectively. Among 2965 DEPs across 5/6 nephrectomized and sham-operated rat kidney tissues, 86 DEPs showed same expression patterns in the urine and kidney tissue. After cross-validation with two external animal proteome datasets, 38 DEPs were organized; only 10 DEPs, including serotransferrin, gelsolin, poly ADP-ribose polymerase 1, neuroblast differentiation-associated protein AHNAK, microtubule-associated protein 4, galectin-1, protein S, thymosin beta-4, myristoylated alanine-rich C-kinase substrate, and vimentin were finalized by screening human GECs and PTECs data. Among these ten potential candidates for universal CKD marker, validation analyses for protein S and galectin-1 were conducted. Galectin-1 was observed to have a significant inverse correlation with renal function as well as higher expression in glomerulus with chronic injury than protein S. This constitutes the first multi-sample proteomics study for identifying key renal-expressed proteins associated with CKD progression. The discovered proteins represent potential markers of chronic renal cell and tissue damage and candidate contributors to CKD pathophysiology.

Ukraine launched a massive drone attack on Moscow overnight, the largest on the Russian capital since the Russian invasion more than two years ago, forcing three airports to divert traffic and causing casualties.

A Rhode Island man with a passion for caffeine earned a Guinness World Record for his collection of 1,019 energy drink cans.

The first step to saving nature is the rewilding of our own minds, says conservationist and former Patagonia CEO Kristine McDivitt Tompkins. With an unwavering commitment to protecting ecosystems, she and her late husband Douglas Tompkins created vast conservation parks across South America that allowed ancient flora and fauna to flourish once again. Now, she's carrying that legacy and mission forward with a bold plan to connect parks across geographic boundaries, creating a system of continental-scale wildlife corridors — before it's too late.

Marc Tucker reviews David Driscoll's new book, 'Commitment and Common Sense', and describes how the Massachusetts reforms are comparable to those in top performing education systems around the world.

Massachusetts' new performance assessment for teacher candidates helps boost readiness.

Relying on newly available data under ESSA, a local advocacy group found several districts that spend more money on wealthy students than poor students, despite the state's intentions.

The state, which earned a B-plus, led the nation in K-12 achievement rankings and outperformed other states in several key academic indicators, but fell short on funding equity.

This Quality Counts 2019 Highlights Report captures all the data you need to assess your state's performance on key educational outcomes.

The state's "Influence 100" project includes a leadership development program that will give aspiring district leaders a hands-on opportunity to work through an equity issue in their home districts.

Massachusetts is the fifth state to join the Innovative Assessment Demonstration Authority created through the Every Student Succeeds Act, which allows states to experiment with new forms of testing.

This Quality Counts 2020 Highlights Report captures all the data you need to assess your state's performance on key educational outcomes.

Fish biomass faces steep falls by end of century under high-emissions scenario

Tod Machover, an MIT professor whose classroom produced Guitar Hero and Rock Band, talks about a future in which everyone can make music

Almost 66 million years ago, an asteroid hit Earth – and changed our planet forever. From tsunamis to shockwaves, join us on a journey through time as we explore the science behind this cataclysmic event. READ MORE about the last day of a dinosaur here: https://www.smithsonianmag.com/science-nature/the-last-day-in-the-life-of-an-edmontosaurus-180979932/ --- For more videos from Smithsonian Magazine: https://www.smithsonianmag.com/videos/ Digital Editorial Director: Brian Wolly Supervising Producer & Scriptwriter: Michelle Mehrtens Video Editor: Sierra Theobald

In a 11-4 vote, the City Council decided to remove the 65-foot-tall monument from its location in the heart of the city

The Galeón Andalucía, which is now making its way to London, was designed to resemble the armed merchant vessels manufactured by Spain and Portugal between the 16th and 18th centuries

Two weeks ago, organizers of Project Skydrop stashed a golden statuette in a secret location somewhere in the northeastern United States

The giant salmon carp was formally identified in 1991, and since then, fewer than 30 individuals had been documented

The insect eggshell is a multifunctional structure with several important roles, including generating an entry point for sperm via the micropyle before oviposition, serving as an oviposition substrate attachment surface, and functioning as a protective layer during embryo development. Eggshell proteins play major roles in eggshell tanning and hardening following oviposition and provide molecular cues that define dorsal–ventral axis formation. Precise eggshell formation during ovarian follicle maturation is critical for normal embryo development and the synthesis of a defective eggshell often gives rise to inviable embryos. Therefore, simple and accurate methods for identifying eggshell proteins will facilitate our understanding of the molecular pathways regulating eggshell formation and the mechanisms underlying normal embryo development. This protocol describes how to isolate and enrich eggshells from mature oocytes of Aedes aegypti mosquitoes and how to extract their eggshell proteins for liquid chromatography with tandem mass spectrometry (LC–MS/MS) proteomic analysis. Although this methodology was developed for studying mosquito eggshells, it may be applicable to eggs from a variety of insects. Mosquitoes are ideal model organisms for this study as their ovarian follicle development and eggshell formation are meticulously regulated by blood feeding and their follicles develop synchronously throughout oogenesis in a time-dependent manner.

When the U.S. Army massacred a Lakota village at Blue Water, dozens of plundered artifacts ended up in the Smithsonian. The unraveling of this long-buried atrocity is forging a path toward reconciliation

SolidWorks ranks fourth, making it the top ranking software company in its category

#1 mainstream 3D mechanical design software provider wins third time in three years since award first announced

Faced with soaring material costs, LeBARON Foundry streamlined designs, lightening manhole covers and frames by as much as 50 pounds

The Learning Company and Dassault Systèmes Form Strategic Licensing Agreement

The three Somerville High School juniors allegedly entered a freshman cabin and sexually assaulted three victims.

Reports of the outages spiked between midnight and 2 am EST, with the network receiving thousands of complaints during this time, according to Down Detector.

Ahead of the film's release, Vikrant made a surprise visit to Godhra railway station on Monday, the site where the Sabarmati incident happened

Vikrant Massey said, "That was the miscommunication, and I should have been a little more careful"

Secretary of State Jeffrey Bullock has suspended the Delaware certified massage technician license of Robert D. Leech for engaging in inappropriate sexual contact with massage clients. In making this determination, Secretary Bullock considered a complaint filed by the Delaware Department of Justice. The complaint states that Mr. Leech used his position to impose unwelcome sexual […]

Defendants placed more than 1.3 billion deceptive fundraising calls claiming to support veterans, children, firefighters The Delaware Department of Justice, along with the Federal Trade Commission and 46 agencies from 38 states and the District of Columbia, has stopped a massive telefunding operation that bombarded 67 million consumers with 1.3 billion deceptive charitable fundraising calls, […]

Three illicit massage establishments (IMEs) were shut down in February, the first full month of operation for the DOJ’s first Human Trafficking Unit (HTU), Attorney General Jennings announced Thursday. “These investigations highlight a continuing pattern that we’ve seen time and time again: human trafficking often happens in plain sight,” said Attorney General Jennings. “These were […]

1. Nasa Alert: Massive 'God of Chaos' asteroid Apophis nears earth in closest pass yet  CNBCTV18
2. NASA alert! 'God of Chaos' asteroid approaching Earth on November 13 sparks worldwide concern  The Times of India
3. Giant Meteorite To Hit Earth On November 13? What We Know  News18
4. 'Astonishingly close': A colossal space rock could wreak havoc on Earth in 2029  Business Today
5. Giant 'God of Darkness' Asteroid May Not Escape Earth Unscathed  ScienceAlert

This post was contributed by Liliko Uchida, application engineer at Cadence. Being a “Woman in STEM” is a phrase that has long been used to describe the holistic experience shared by thousands of women globally, yet it still makes us feel isolated. Partially due to the statistics of gender population in the STEM workforce and the remainder due to our own internal obstacles, being a woman in STEM continues to be a challenge. While many of us know the should-do’s and should-be’s of taking on this unique role objectively, we struggle to implement them. After all, our perseverance as engineers, mathematicians, businesswomen, programmers, and scientists is largely affected by subjectivity. The UMass Lowell Women’s Leadership Conference 2024 aimed to tackle this problem by uniting hundreds of women with shared experiences under one roof. Not only did the conference provide us with the knowledge necessary to persevere, but it also gave us the tools that will allow us to thrive and act upon the facts we already know. It is my hope that through this blog post, I can share some of my main takeaways from this special day. Be Confident This is one of the most palpable pieces of advice we always hear. Yet so many of us struggle to build this confidence because we don’t know how. Featured speaker Nicole Kalil defined confidence as “complete trust in oneself”.”One way to build this self-trust is by getting to know yourself on a deeper level. By creating a true inner connection, we begin to see ourselves as a whole instead of hyper-focusing on our shortcomings frequently illusioned by imposter syndrome. In one of the sessions, we were asked to introduce ourselves to our neighbors, not by what we do for work, but by who we are as a person. Even if this opportunity does not arise every day, this practice can be done simply by listing characteristics of yourself that define who you are. Who do you care for? How do you show them? What are your life goals oriented towards? How do you observe others’ behavior around you, and what does that say about how you make them feel? Getting to know you beneath the surface and allowing yourself to be seen for who you are is critical in building internal confidence. With practice, this self-reassurance will grow independent of external factors. Take Risks “Sometimes, you have to put your foot in the elevator” - Barb Vlacich, Keynote Speaker When opportunities arise, the only thing you can do to have a chance is to try. Without putting your foot in the elevator, the doors will close, becoming a missed opportunity. Similarly, several of the conference’s speakers also emphasized that the answer to every unasked question will always be a no. Even if you are not ready to full-send a negotiation, ask for a raise, or respectfully disagree with a co-worker’s opinion, start by getting comfortable asking uncomfortable questions. Just one discomfort a day will help in building an immunity to the anxiety that comes with taking risks, typically driven by our self-doubt. Another interesting point that stood out from the conference was the statistics of self-assessed qualifications between men and women. During the negotiation panel, it was revealed that men typically feel they only need 60% of the qualifications under a job description to apply, whereas women often feel they need close to 100%. These numbers alone demonstrate how the pure mental habits of men continue to funnel them into STEM and not women. The next time you seek a new opportunity, assess yourself based on the 60% and use it as a checklist threshold. If more women are able to pursue STEM careers using these numbers, the more likely we will begin to populate these roles. Build Your Genuine Network “ The essence of communication lies in the mutual exchange of ideas and emotions. And when the listener isn’t invested, it undermines the entire purpose of the conversation. Why are you having it anyway?” This is a quote from episode 186 of Julie Brown’s podcast This Sh!t Works called “The 5 Steps to Being an Active Listener”. Julie Brown is a Networking Coach, author, and podcast host who guided an energetic and candid conversation about networking and building a personal brand for women. Networking is often misunderstood as putting your name and qualifications out on the table for as many people to pick up your cards. While making these things known is important, they are not what nurtures effective connections. The key to cultivating your genuine network is to activate a sincere interest in the people you meet. Become the proactive receiver of the confidence exercise discussed above. When you meet someone new, what can you take away from them as a person, not an employee? By making people feel heard, even through the little conversations, you can begin to develop more meaningful connections that resonate. And, with practice, the sometimes inherent need to overcompensate by defining yourself with your resume will slowly fade. It was a wonderful opportunity to attend the UML Women’s Leadership Conference with four other inspiring Cadence women. Not only was the conference a motivating learning experience, but it was also a wonderful opportunity for us to bond together as women and feel supported by each other. The most eye-opening part of the day was seeing just how many women alike were sitting under the same roof. The conclusion of the event led me to feel proud to be an engineer, proud to be at Cadence, and most importantly, proud to be a woman. Learn more about life at Cadence .

Abuja, Nigeria — The Nigerian military welcomed a report by a human rights group that found “no evidence” the military secretly carried out thousands of illegal abortions as part of its fight against Islamist insurgency. The Reuters news agency says it stands by its three reports in 2022 which lodged the allegations.  In a statement over the weekend, Nigerian Defense Chief General Christopher Musa praised the report by the seven-member investigative panel set up by the National Human Rights Commission of Nigeria.  Musa said no amount of false accusations will distract the armed forces from defending Nigeria's integrity.  On Friday, the Nigerian investigative panel said it found “no evidence” that the Nigerian military secretly carried out illegal abortions or had such a policy in its operations against terror groups.   The panel's probe lasted 18 months and was based on 199 testimonies from witnesses including Musa, who was leading the counterinsurgency campaign at the time.  In December 2022, Reuters news agency published three reports alleging that the Nigerian military ran a mass abortion program for victims of the long-running Boko Haram insurgency and massacred children in the northeast.  The report alleged that the military had carried out more than 10,000 abortions since 2013.  "The panel made its findings meticulously on each of the issues," said Hillary Ogbonna, secretary to the investigative panel. "For the abortion assault, the panel did not find evidence that the Nigerian armed forces committed a systematic, secret or policy-driven abortion in the northeast to the tune of 10,000 abortions."  The committee, however, agreed with a Reuters report alleging the military committed human rights violations, including infanticide, during a military operation in June 2016.  "The panel found the military culpable of infanticide and the killing of community members in the village of Abisare in Borno State. We've received witness testimonies including those of the survivors and relations of the deceased," Ogbonna said.  Responding to the panel's report, Reuters said it stands by its investigation, stating that it satisfied their standards for independence, accuracy and impartiality.  On Monday, a Nigerian human rights group, Women Concerns, called on Reuters to withdraw the report and apologize to the Nigerian military.  But security analyst Chidi Omeje said when there's disagreement between two parties, an independent committee can be set up to review the reports.  "I don't know how the human rights commission went about the investigation. I really cannot say whether it was a thorough job done or whether there was some influence somewhere. Maybe some other independent organizations should be able to wade in," Omeje said. "I know that [the] human rights commission, even though I'm not trying to doubt their integrity, it has some allegiance to the government because it's a government agency."  Years of military offensives against terror groups in Nigeria have been embroiled in allegations of human rights violations. Soldiers have been accused of mistreating civilians and carrying out extrajudicial killings.  "It tells you how it is very difficult to maintain or stick to that professionalism if you're dealing with this kind of asymmetric warfare. Sometimes it is difficult to distinguish who the adversaries are," Omeje said.  Recently, the military launched a public relations campaign, seeking to repair its reputation.

While Ukraine was on high alert for air attacks on Monday, the country’s top military commander said tens of thousands of Russian troops were ready to advance on the Kursk region. "Following the order of their military leadership, they are trying to dislodge our troops and advance deep into the territory we control," Ukraine’s General Oleksandr Syrskyi wrote on the Telegram messaging app. Ukraine launched an incursion into Kursk in August, taking control of a number of towns and villages in the border region. Meanwhile, at least six people were killed in air attacks in southern Ukraine – five in Mykolaiv and one in Zaporizhzhia, where a residential building was destroyed, regional governors reported. At least a dozen people were injured in Zaporizhzhia, including five children between the ages of 4 and 17. Earlier, the Ukrainian air force put the nation on high alert for a large-scale missile attack, reporting that a large number of bombers were taking off from Russia and heading to Ukraine. "The air alert is related to the launch of cruise missiles from Tu-95MS strategic bombers," the air force said on its Telegram channels. Power was cut to prevent further damage from attacks, and people were encouraged to seek shelter – including in Kyiv’s metro stations. But by 0630 GMT the missiles had not arrived. According to some Ukrainian military bloggers, the Russian bombers performed flights imitating the launch of missiles, Reuters reported. Report: Trump urges Putin not to escalate The Washington Post and Reuters reported that U.S. President-elect Donald Trump spoke to Russian leader Vladimir Putin and urged him not to escalate the war in Ukraine. Trump, calling from his Mar-a-Lago estate in Florida on Thursday, reminded Putin of America's sizable military presence in Europe, the Washington Post reported. Sources familiar with the call told the newspaper Trump expressed an interest in further conversations to discuss "the resolution of Ukraine's war soon." Steven Cheung, Trump's communications director, did not confirm the exchange, saying in a written statement to AFP that "we do not comment on private calls between President Trump and other world leaders." The Kremlin on Monday denied that the conversation took place, and said Putin had no concrete plans to speak to Trump. "This is completely untrue. This is pure fiction, it's just false information," Reuters reported Kremlin spokesman Dmitry Peskov said. "There was no conversation." Information from Reuters and Agence France-Presse was included in this report.  

On July 10, 1941, Christian Poles hunted down, clubbed, drowned, gutted, and burned alive 1,600 Jewish men, women, and children-all but seven of the town's Jews.

Geneva — U.N. High Commissioner for Human Rights Michelle Bachelet on Thursday cast doubt on whether she will release a long-awaited report on the mass incarceration of Uyghurs in China's Xinjiang region before she leaves office on August 31. When she announced her departure in June, Bachelet said she would publish the report before her term ended. In her final briefing as high commissioner, she said she hoped it would be possible but indicated its release, once again, might be delayed. Bachelet said her office has received substantial input from the Chinese government that must be carefully reviewed before the report can be issued. She said that was normal procedure for all country reports published by her office. "In my meeting with high level national officials and regional authorities in Xinjiang, I raised concern about human rights violations, including reports of arbitrary detention and ill-treatment in institutions," she said. "And the report looks in depth on to these and other serious human rights violations concerning the Uyghurs and other predominantly Muslim minorities in Xinjiang." Human rights activists accuse China of the mass detention, torture, and cultural persecution of a million Uyghurs and other Turkic Muslims in so-called vocational camps. China denies the allegations, saying people in training centers receive skills they need to get good jobs. Bachelet said she raised many concerns with Chinese authorities during her visit to Xinjiang in May. In July, the Reuters news agency reported that China had sent Bachelet a letter asking her not to publish the report. She has confirmed receipt of that letter, which was signed by diplomats of some 40 countries. The high commissioner said such solicitations from countries under the human rights spotlight are not unusual, adding she does not give in to pressure. "I have been receiving pressures from countries who want to publish or not to publish," Bachelet said. "You cannot imagine the numbers of letters, meetings asking for the non-publication. Huge numbers … I have been under tremendous pressure to publish or not to publish. But I will not publish or withhold publication due to any such pressure. I can assure you of that." Work on the report has been ongoing for the past three years. The high commissioner has one week left on her mandate. She assured journalists that she was trying very hard to do what she had promised, namely to release the report before she leaves on August 31.