Disorders of oxygen transport are commonly attributed to inadequate carrying capacity (anemia) but may also relate to inefficient gas exchange by red blood cells (RBCs), a process that is poorly characterized yet assumed to be rapid. Without direct measurements of gas exchange at the single-cell level, the barriers to O2...

Triple-negative breast cancer (TNBC) accounts for 10 to 20% of breast cancer, with chemotherapy as its mainstay of treatment due to lack of well-defined targets, and recent genomic sequencing studies have revealed a paucity of TNBC-specific mutations. Recurrent gene fusions comprise a class of viable genetic targets in solid tumors;...

Neural networks have become the method of choice in surrogate modeling because of their ability to characterize arbitrary, high-dimensional functions in a data-driven fashion. This paper advocates for the training of surrogates that are 1) consistent with the physical manifold, resulting in physically meaningful predictions, and 2) cyclically consistent with...

Yen-Ling Lian, Kuan-Wei Chen, Yu-Ting Chou, Ting-Ling Ke, Bi-Chang Chen, Yu-Chun Lin, and Linyi Chen

Myoblast fusion is required for myotube formation during myogenesis, and defects in myoblast differentiation and fusion have been implicated in a number of diseases, including human rhabdomyosarcoma. Although transcriptional regulation of the myogenic program has been studied extensively, the mechanisms controlling myoblast fusion remain largely unknown. This study identified and characterized the dynamics of a distinct class of blebs, termed bubbling blebs, which are smaller than those that participate in migration. The formation of these bubbling blebs occurred during differentiation and decreased alongside a decline in phosphatidylinositol-(3,4,5)-trisphosphate (PIP3) at the plasma membrane before myoblast fusion. In a human rhabdomyosarcoma-derived (RD) cell line that exhibits strong blebbing dynamics and myoblast fusion defects, PIP3 was constitutively abundant on the membrane during myogenesis. Targeting phosphatase and tensin homolog (PTEN) to the plasma membrane reduced PIP3 levels, inhibited bubbling blebs and rescued myoblast fusion defects in RD cells. These findings highlight the differential distribution and crucial role of PIP3 during myoblast fusion and reveal a novel mechanism underlying myogenesis defects in human rhabdomyosarcoma.

Mutation and translocation of fibroblast growth factor receptors often lead to aberrant signaling and cancer. This work focuses on the t(8;22)(p11;q11) chromosomal translocation which creates the breakpoint cluster region (BCR) fibroblast growth factor receptor1 (FGFR1) (BCR-FGFR1) fusion protein. This fusion occurs in stem cell leukemia/lymphoma, which can progress to atypical chronic myeloid leukemia, acute myeloid leukemia, or B-cell lymphoma. This work focuses on the biochemical characterization of BCR-FGFR1 and identification of novel therapeutic targets. The tyrosine kinase activity of FGFR1 is required for biological activity as shown using transformation assays, interleukin-3 independent cell proliferation, and liquid chromatography/mass spectroscopy analyses. Furthermore, BCR contributes a coiled-coil oligomerization domain, also essential for oncogenic transformation by BCR-FGFR1. The importance of salt bridge formation within the coiled-coil domain is demonstrated, as disruption of three salt bridges abrogates cellular transforming ability. Lastly, BCR-FGFR1 acts as a client of the chaperonin heat shock protein 90 (Hsp90), suggesting that BCR-FGFR1 relies on Hsp90 complex to evade proteasomal degradation. Transformed cells expressing BCR-FGFR1 are sensitive to the Hsp90 inhibitor Ganetespib, and also respond to combined treatment with Ganetespib plus the FGFR inhibitor BGJ398. Collectively, these data suggest novel therapeutic approaches for future stem cell leukemia/lymphoma treatment: inhibition of BCR oligomerization by disruption of required salt bridges; and inhibition of the chaperonin Hsp90 complex.

We report the diagnostic challenges and the clinical course of a patient with an extraordinary presentation of B-lymphoblastic leukemia (B-ALL) with eosinophilia. We identified a novel ZBTB20-JAK2 gene fusion as a chimeric RNA transcript using the Archer platform. This gene fusion from the same patient was recently identified by Peterson et al. (2019) at the genomic level using a different sequencing technology platform. The configuration of this gene fusion predicts the production of a kinase-activating JAK2 fusion protein, which would normally lead to a diagnosis of Philadelphia chromosome–like B-ALL (Ph-like B-ALL). However, the unusual presentation of eosinophilia led us to demonstrate the presence of this gene fusion in nonlymphoid hematopoietic cells by fluorescence in situ hybridization (FISH) studies with morphologic correlation. Therefore, we believe this disease, in fact, represents blast crisis arising from an underlying myeloid neoplasm with JAK2 rearrangements. This case illustrates the difficulty in differentiating Ph-like B-ALL and myeloid/lymphoid neoplasm with eosinophilia and gene rearrangements (MLN-EGR) in blast crisis. As currently defined, the diagnosis of MLN-EGR relies on the hematologic presentations and the identification of marker gene fusions (including PCM1-JAK2, ETV6-JAK2, and BCR-JAK2). However, these same gene fusions, when limited to B-lymphoblasts, also define Ph-like B-ALL. Yet, our case does not conform to either condition. Therefore, the assessment for lineage restriction of gene rearrangements to reflect the pathophysiologic difference between B-ALL and MLN-EGR in blast crisis is likely a more robust diagnostic approach and allows the inclusion of MLN-EGR with novel gene fusions.

BACKGROUND AND PURPOSE:

Diffuse intrinsic pontine glioma is a lethal childhood brain cancer with dismal prognosis and MR imaging is the primary methodology used for diagnosis and monitoring. Our aim was to determine whether advanced diffusion, perfusion, and permeability MR imaging metrics predict survival and pseudoprogression in children with newly diagnosed diffuse intrinsic pontine glioma.

BACKGROUND AND PURPOSE:

Anoxic brain injury is a result of prolonged hypoxia. We sought to describe the nonquantitative arterial spin-labeling perfusion imaging patterns of anoxic brain injury, characterize the relationship of arterial spin-labeling and DWI, and evaluate the normalized diffusion-to-perfusion ratio to differentiate patients with anoxic brain injury from healthy controls.

Within months researchers will attempt to create a ball of plasma hotter than the sun inside the Joint European Torus, a doughnut-shaped machine in south-east England

Government should tell the people clearly about the exact state of the pandemic to enable them to prepare accordingly, said Congress senior spokesperson Ajay Maken.

Follow our live coronavirus updates here Coronavirus: the symptoms

The Government's new portal for arranging coronavirus tests has sparked confusion today, following a message suggesting home tests had run out by 9am.

Bristol Myers Squibb and Acceleron Pharma’s Reblozyl (luspatercept) has secured a positivr opinion from the European Medicines Agency’s Committee for Medicinal Products for Human Use (CHMP) for use in the treatment of transfusion-dependent anaemia in two adult patient populations.

Two Miami-area residents pleaded guilty today in connection with a $10 million Medicare fraud scheme involving HIV infusion clinics. Dr. Carmen Del Cueto, 65, and Alexis Dagnesses, 44, each pleaded guilty to one count of conspiracy to commit health care fraud before U.S. District Judge Paul C. Huck. Both defendants admitted to working at Midway Medical Center Inc. (Midway), a Miami clinic that purported to specialize in the treatment of patients with HIV.

Miami physician Keith Russell, 65, and physician’s assistant Jorge Luis Pacheco, 50, were each sentenced to 97 months in prison, and physician’s assistant Eda Marietta Milanes, 43, was sentenced to 63 months in prison, for their roles in fraud schemes that involved billing Medicare for $10,903,509 worth of unnecessary HIV infusion treatments.

Three Miami-Dade County, Fla., residents have been indicted in connection with an alleged $2.3 million Medicare fraud scheme operated out of X-Press Center, a Detroit-area clinic that purported to specialize in providing injection and infusion therapies.

Miami-Dade County resident Dulce Briceño was sentenced today to 63 months in prison for her role in a $2.3 million Medicare fraud scheme.

"I’m proud of what’s been accomplished, in a very short time, by our fusion centers," said Attorney General Holder.

Miriam Freytes, 49, pleaded guilty today to one count of conspiracy to commit health care fraud before U.S. District Court Judge Denise Page Hood of the Eastern District of Michigan.

David Marrero, 49, was convicted today of health care fraud, conspiracy to commit money laundering and money laundering in connection with a $5.8 million Medicare fraud scheme relating to his involvement with a Miami-area HIV/AIDS infusion clinic.

Modesto and Victoria de la Vega pleaded guilty today in U.S. District Court in Miami for their participation in a $13.7 million HIV infusion Medicare fraud scheme.

David Marrero, a founder of and consultant at a fraudulent Miami-area HIV/AIDS infusion clinic known as Tendercare Medical Center Inc., was sentenced today to 10 years in prison for his role in a $5.8 million scheme to defraud the Medicare program.

Two brothers pleaded guilty today in U.S. District Court in Miami for participating in a $13.7 million HIV infusion Medicare fraud scheme.

An owner and a physician associated with a Detroit-area infusion therapy clinic were sentenced to 120 months and 97 months in prison, respectively, for their leading roles in a $2.3 million Medicare fraud scheme.

A patient recruiter for three Detroit-area clinics was sentenced to 27 months in prison for his role in fraud schemes that attempted to defraud the Medicare program of more than $15 million.

Two owners and operators of clinics that claimed to specialize in treating HIV and other conditions pleaded guilty today for their roles in an infusion therapy scheme carried out at two Detroit-area clinics that submitted millions of dollars in fraudulent claims to Medicare.

A former Miami HIV infusion clinic director was sentenced today to serve 70 months in prison for his role in a $26.2 million HIV infusion fraud scheme.

An unlicensed nurse who fled after being charged in 2008 and was captured this year was sentenced today to serve 108 months in prison.

CareFusion Corp. has agreed to pay the government $40.1 million to settle allegations that it violated the False Claims Act by paying kickbacks and promoting its products for uses that were not approved by the Food and Drug Administration.

Fusing therapeutic proteins to affibodies with pH-dependent binding to neonatal Fc receptor could improve the proteins' half-lives.

There remains confusion over how many teams will be on the grid in the season-opener in Bahrain next wee

MIT design cuts fusion reactor down to a size that would be more cost effective and could be built in five years

The GoSun Fusion probably won’t save the world, but the design thinking behind it might.

Scientists at the National Ignition Facility (NIF) achieve a major breakthrough on the path to the dream of fusion as a clean energy source

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The new coronavirus relief law extends unemployment benefits to gig, self-employed and other previously ineligible workers, but they may have to wait to file and receive benefits.

London embassy contradicts health minister as visitors could face 14-day confinement even if asymptomatic

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French officials have announced that Britons and those from European Union member states will not be placed in 14 days mandatory quarantine if they travel to France, as suggested by the country’s health minister.

In what appeared to be a direct contradiction of Olivier Véran’s statement on Saturday, the French embassy in London tweeted that the measure would not be imposed on those arriving in France from the UK or Schengen area.

Related: France to sell some of nation's antique furniture to support hospitals

Monday was a confusing mess with the BMC not issuing clear orders on the reopening of shops and liquor stores. Many ward officers did not allow any shops to open until orders came from the municipal commissioner and clarification on what shops can remain open on one street.

Wine shops in Dahisar were not allowed to reopen. "We haven't received any circular, guidelines from the headquarters. Until that happens, we can't allow non-essential or wine shops to open," said Sandhya Nandedkar, assistant commissioner of R North ward. In Dadar-Worli area, wine shops were closed. "There isn't any order from the BMC. The shops can't open without proper permissions," said Prakash Patankar, ex-corporator from Dadar.

A crowd outside a wine shop in Walkeshwar on Monday. Pic/Bipin Kokate

"The state's order said that not more than five shops can open on one road. How can we select the shops? Shop-owners who want to resume business should apply to us," said a senior BMC officer. But the BMC has not issued a circular regarding such applications. Even BMC officers were confused over action on wine shops, some of which were open throughout the day, without the corporation's order.

Shopkeepers exasperated
A Worli shop-owner said, "Whose orders are to be followed? There is a lot of confusion, we are already in a mess and what the government is doing is harassment."

"The new MHA notification is a good start to get the economy moving. Mumbai has rules apart from the state notification — of only opening five non-essential stores per lane. There is a requirement to get further clearances from local authorities," said Kumar Rajagopalan, CEO, Retailers Association of India.

People wait to buy liquor outside a shop in Mulund. Pic/Rajesh Gupta

Municipal chief Praveen Pardeshi was not available for comment.

Early birds get nothing
Wine shops across the city saw people queuing up right from 6 am on Monday, expecting to quickly procure the available stock. Amid confusion over the reopening, police eventually dispersed everyone. Queues were seen in Lalbaug, Parel, Kalachowkie, Matunga, Sion and Mahim.

On Monday morning, Sachin Jadhav, standing outside Circle wine shop in Matunga, said, "I have been without alcohol for 45 days. I am ready to consume it." Another shopper, Rajesh Pandit, said, "The shop has been closed for a month and a half. There is a fear that it will run out of stock very soon. Which is why I got up early in the morning."

People queue up to buy liquor in Vile Parle East. Pic/Rane Ashish

Quiet Bandra
In Bandra, most groceries and pharmacies operated as per usual. A couple of shops selling electronic items kept shutters half open. No stationery or hardware shops were open. An optician on Hill Road was among the few shops open to regular customers. Owner Piyush Chheda said he opened shop even though there isn't much clarity on rules. "Customers have been calling regarding their orders. It is an essential item. I opened the shop at 10:30 am and will close at 2:30 pm," said Chheda.

A garment store, Fascination, was open near Khar Pali Road. Owner Shiv Dang said, "We opened the shop at noon and will be open till 7 pm. We called only half the staffers who live nearby." Dang did not receive any communication from BMC.

H West ward officials said they haven't received orders from the BMC. "We are hoping to get directions from senior officials by the end of the day about which five shops may remain open. We will inform shop-owners accordingly," said an official.

While most wine shops were shut, Silver Coin Wines on Ambedkar Road opened and saw a crowd of at least 100, some of them without a mask.

Chaotic Mulund wine shops
In Mulund, shops opened in the morning but shut down around noon, with only pharmacies allowed to remain open. "We have been open all this while and our business remains unaffected. Though supply is limited to important medicines," said a pharmacist.

A liquor shop at Ambedkar Road, Bandra on Monday morning

Wine shops were the main attraction with complete chaos, long queues, no social distancing. Shops at Mulund check naka, LBS Road, Veena Nagar had long queues and crowds.

Grocers followed the usual specified timings. Milind Gala from Mulund East said, "Though all kinds of shops have been allowed to remain open, supply is limited." The limited timings also saw more crowds.

A few stationery vendors said there had been no orders from local civic officials or police on reopening. "There is too much confusion and we will end up losing our licence. Hence, we decided to remain closed," said Jayeshbhai from Mulund west.

Why open wine shops?
Mulund resident Keshav Madhukar said, "Though the easing of the lockdown is welcome, there seems to be confusion among heads of departments in implementing orders. There is no clarity on how things will work."

Another resident Gaurav Kumar said, "At the check naka in Mulund, there were crowds at liquor shops. Is this supposed to be a lockdown in a red zone?"

Another citizen Risshita Gada said, "Was it so important to open wine shops? How will social distancing be followed?"

Later in the day, BMC's T Ward tweeted clarifying that except for essential commodity shops, no other shops were allowed to open.

Mulund MLA Mihir Kotecha said, "I have put up details of which shops will remain open and which won't. I have also met around 140 housing society secretaries via video conferencing to discuss the way forward. If you need to step out, make a proper plan. List things up and fetch all of it in one go."

Borivli residents saddened
Residents of Borivli were rather disappointed as absolutely no wine shops were open. Among the non-essential shops allowed to open, only two- and four-wheeler mechanics, sweets and farsan shops and small stationary outlets which had photocopy machines were allowed to open. A few hardware and electronic repair shops were open, while groceries and pharmacies functioned as usual.

Residents also queued up outside wine shops early morning. "People started queuing up from 8.30-9 am. The crowd soon left as shops did not open. But people kept checking in throughout the day," said Jitesh Jain, who runs a grocery shop in I C Colony, where two to three wine shops are located nearby. "Looking at what has happened at other wine shops, it is good that the ones here were closed," said a customer, Harish Ganega who was in the queue at Jain's shop.

Other non-essential shop-owners remained confused. Dheeraj Purohit, owner of Purohit sweets and farsan shop outside Borivli station, said, "We opened today and will most likely shut by lunch time. We wanted to see how it works, whether social distancing is possible. There is similar confusion among several shop-owners which is why many are closed."

Mira Road groceries reopen
Grocery shops opened after 13 days in Mira Road and saw long queues. The Mira Bhayandar Municipal Corporation (MBMC) had imposed a complete lockdown from April 20 to May 2. Only milk booths and pharmacies were allowed to remain open for a few hours.

Other than groceries and bakeries, no other shops were allowed to open. Milk booths were open till 11 am and pharmacies till 9 pm.

MBMC allowed grocery and bakery shops to remain open from 9 am to 3 pm. Vegetable vendors are still not allowed on roads or in open spaces and MBMC has permitted only home delivery. "We bought enough groceries last month, but some items like tea powder, groundnuts are finished. How can we ask for home deliveries for such a small order?" said a customer in a queue at Shiv Shakti shop. Another woman said that while groceries were giving home delivery, shops selling various flours, spices and coconuts were closed.

Andheri comes alive
Andheri seemed lively and bustling since morning. Although not all wine shops were open, the ones that were, like Pinky Wines in Oshiwara, had serpentine queues stretching more than 200 metres. People even waited throughout the afternoon when the shop was closed for lunch. General stores had longer queues than usual.

There was a visible increase in the number of vehicles plying between Andheri and Juhu. Lokhandwala Market had numerous cars double parked on the road. Four bungalows too, was packed and bustling till around noon, but slowed down after cops began patrolling.

13
No. of days after which groceries opened in Mira Road

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New medication will decrease the need for blood transfusions in thalassemia patients, and eventually improve bone-marrow transplant outcomes, according

Company: Winvest Technology Consultancy Services Private Limited
Experience: 2 to 8
location: Hyderabad / Secunderabad
Ref: 24827973
Summary: IPA Workfusion with JAVA, Selenium ,Machine Learning

This paper analyses the characteristics of firms that operate at the global productivity frontier and their relationship with other firms in the economy, focusing on the diffusion of global productivity gains and the policies that faciliate it.

Austria’s transition to a digital economy and society is slower than in other high-income small open European economies.

This paper uses a novel data set of digital technology usage covering 25 industries in 25 European countries over the 2010-16 period to explore the drivers of digital adoption across two broad sets of digital technologies by firms, cloud computing and back or front office integration.