The sacral-iliac joint between an iliac and a sacrum is fused either by the creation of a lateral insertion path laterally through the ilium, through the sacral-iliac joint, and into the sacrum, or by the creation of a postero-lateral insertion path entering from a posterior iliac spine of an ilium, angling through the sacral-iliac joint, and terminating in the sacral alae. A bone fixation implant is inserted through the insertion path and anchored in the interior region of the sacrum or sacral alea to fixate the sacral-iliac joint.

An elongate inserter has a distal end releasably connected to an expandable interbody fusion device and a proximal end including a trigger actuator. The interbody fusion device comprises a superior endplate and an inferior endplate that are movable in an expansion direction relative to each other in the intradiscal space. The inserter includes a lifting platform and an elevator that define cooperatively engaging ramps and ramps surfaces that upon operation of the trigger actuator cause the superior and inferior endplates to move relatively away from each other. A driver is supported by the inserter for pushing an insert into the expanded device between the superior and inferior endplates.

According to typical practice of the present invention, temporal identifiers are simultaneously associated with (i) video and (ii) numerical data while these data are contemporaneously collected by, respectively, (i) a video camera filming an event and (ii) a data acquisition system acquiring numerical data from sensor(s) obtaining sensory information relating to the event. Various modes of inventive practice provide for time codes and/or markers as the temporal identifiers. The video and the numerical data are each converted to a digital form that furthers their mutual compatibility, the video to a compressed/encoded video file, the numerical data to an Adobe XMP data file. The compressed/encoded video file and the XMP data file are merged whereby the temporal identifiers are aligned with each other. The merged video file has numerical data embedded therein and is displayable so that the video and the numerical data are synchronized in comportment with actual real-time occurrence.

A multiple tool device, particularly adapted to prepare tubular elements to be welded by electrofusion, comprising a cup member which is associable with the end portion of a tubular element and is provided with motion transmission means adapted to turn the multiple tool device. The particularity of the present invention resides in that it comprises scraper means adapted to remove the outer surface layer of the end portion and facing means which have a working motion that is opposite with respect to the preceding means and are adapted to face the edge of the end portion so as to make it perpendicular to the axis of the tubular element.

An optical fiber fusion splicer includes: a windshield cover having a rotating shaft and rotating around the rotating shaft so as to be openable and closable; a cable member wrapping unit coupled and fixed to the windshield cover on the same axis as the rotating shaft of the windshield cover or formed as a portion of the windshield cover, the cable member wrapping unit being rotatable in a normal direction or in a reverse direction around the rotating shaft; a first cable member winding unit that is a rotary pulley, a non-rotary pulley, or a rotary gear; and a closed-loop member including a deformable cable member wound around the cable member wrapping unit and the first cable member winding unit, the closed-loop member constituting a closed loop.

An optical fiber fusion splicer that heats and fusion-splices optical fibers to each other, the optical fiber fusion splicer includes: a coating clamp installation base; a coating clamp that is attached to the coating clamp installation base and has a coating clamp lid that is openable and closable; and a first power source for advancing the coating clamp installation base and opening the coating clamp lid. An operation of opening the coating clamp lid is performed using the first power source after the fusion splicing is completed.

Disclosed is a system for extracting energy from inertial confinement fusion reactions, which includes a central target chamber for receiving fusion target material. A plurality of energy drivers are arranged around the target chamber so as to supply energy to fusion target material in the chamber to initiate an inertial confinement fusion reaction of the material, releasing energy in the forms of fusion plasma and heat. A plurality of structures for extracting energy from the fusion reaction are provided, and comprise devices to extract high voltage DC energy from the fusion plasma, and means to extract thermal energy from the central target chamber. Power to the energy drivers may be supplied from high voltage DC energy extracted from the fusion reactions. The energy drivers may use an apodizing filter to impart a desired shape to the wavefront of the driving energy for causing the fusion reactions, to avoid hydrodynamic instabilities.

A system and method determine when fluid is not flowing properly from a secondary infusion source during a secondary infusion. The system includes an upstream pressure sensor and a processor programmed to receive signals from the sensor and analyze the signals to determine if secondary fluid flow is proper. The processor samples the output signals from the upstream pressure sensor and analyzes the sampled signals to determine if a pressure rise in the infusion line has occurred when the secondary infusion is initiated. If a pressure rise, indicating that fluid from the secondary container has begun flowing into the infusion line, has not been detected, the processor is programmed to provide a signal indicating that attention should be given to the infusion set up.

One embodiment includes a method comprising the steps of providing a first dry catalyst coated gas diffusion media layer, depositing a wet first proton exchange membrane layer over the first catalyst coated gas diffusion media layer to form a first proton exchange membrane layer; providing a second dry catalyst coated gas diffusion media layer; contacting the second dry catalyst coated gas diffusion media layer with the first proton exchange membrane layer; and hot pressing together the first and second dry catalyst coated gas diffusion media layers with the wet proton exchange membrane layer therebetween.

A diffusion device for atomizing liquids includes a floating magnet disposed within a track inside at least one package with liquid for the atomizing diffusion device, wherein as a liquid level inside the package changes, the floating magnet moves substantially vertically along the track, a plurality of Hall effect sensors or Hall effect switches disposed outside the liquid at various positions, the Hall effect sensors or Hall effect switches each generating an output voltage in response to a magnetic field of the floating magnet, and a processor that receives the output voltages and fits them to a model that includes the floating magnet position with respect to various sets of output voltages to determine a position of the floating magnet with respect to the Hall effect sensors.

According to embodiments of the present invention, a flameless candle includes a housing, a reservoir, and a lamp. The reservoir can retain a fragrant source (e.g., solid, liquid, or mix thereof. The housing has a hollow region and a recessed area within the top surface of the housing. The recessed area is bounded by a bottom surface and a sidewall. There one or more vent holes in the sidewall. If there is a plurality of vent holes, then they may encircle the sidewall. The vent hole(s) allow an airflow from the hollow region and into the recessed area above the fragrant source. The lamp can emit a light through the bottom surface of the recessed area and through fragrant source.

A fluid infusion device is provided with a cannula spring which functions as an introducer needle, a retraction return spring, and a fluid path. A hollow cannula tubing is wound, bent and sharpened into a shape which allows it to operate as an introducer needle, retraction spring and fluid path in an infusion device. A button is used to insert the introducer needle portion of the cannula spring and a soft catheter, and once the introducer needle portion and catheter have been fully inserted, an engagement between the button and post of the base of the infusion device releases the cannula spring such that the introducer needle portion of the cannula spring automatically retracts, leaving the catheter in the body. An end of the introducer needle portion of the cannula spring remains in fluid communication with the catheter in the body to provide an uninterrupted fluid path.

Valves, valved fluid transfer devices and ambulatory infusion devices including the same.

Valves, valved fluid transfer devices and ambulatory infusion devices including the same.

An infusion pump system providing therapy to a patient in a closed-loop or semi-closed loop mode can safely automatically revert to open-loop therapy. The system stores a default open-loop basal rate profile in memory. The system also continually tracks the insulin on board for the patient over a plurality of closed-loop therapy intervals. When an error or event occurs requiring reversion to open-loop therapy, the system automatically provides therapy according to the open-loop basal rate profile and the tracked insulin on board amount.

Disclosed herein is a fluid infusion device of the type that delivers medication fluid to the body of a patient. The device includes or cooperates with a fluid reservoir, and the device has a sealing assembly to receive and form a fluid seal with the fluid reservoir. A retractable sealing element surrounding a hollow fluid delivery needle may be used to seal a port of the fluid reservoir. The port may include a pressure vent that is sealed by the retractable sealing element. In one variation, the reservoir includes a moving valve sleeve that holds a septum. The septum moves to allow the reservoir to vent, and to form a seal with the port when the needle pierces the septum. In another variation, the device includes a needleless sealing assembly. In yet other variations, the device uses a needled fluid reservoir.

The present invention discloses a system and method for monitoring an infusion pump capable of intelligently easing pain. Each infusion pump control terminal is connected with a monitoring server through a wireless AP and a local area network respectively; each human body vital sign sensor is connected with the signal input end of a field programmable gate array FPGA through a sensor interface circuit respectively, an infusion control device is connected with the control signal output end of the field programmable gate array FPGA, the field programmable gate array FPGA is in communication with an ARM processor in a bus coding mode, and the ARM processor is in communication connection with the wireless AP through a WIFI communication module. By means of the system and method for monitoring infusion pump capable of intelligently easing pain, a plurality of basic vital sign data of a patient is collected in real time, corresponding infusion schemes are generated through analysis of the data, the infusion pump is controlled to achieve automatic infusion, monitoring and pain-easing infusion are combined together for coordinative work, and infusion control is more scientific and reliable; patient online perception and feedback is supported, self-improvement of a system is facilitated, and more accurate and reliable infusion schemes can be acquired.

A method identifies to which one of a plurality of infusion pumps one of a plurality of fluid lines is coupled. The method can include intentionally producing a predetermined pressure pattern in one of the plurality of fluid lines, detecting the predetermined pressure pattern by way of a sensor of one of the plurality of infusion pumps, and indicating detection of the predetermined pressure pattern in the one of the plurality of fluid lines, thereby indicating the one of the plurality of infusion pumps to which the one of the plurality of fluid lines is coupled. In some cases, a tool configured to occlude and the squeeze the fluid line can be used to intentionally produce the predetermined pressure pattern.

Disclosed are methods and compositions for detecting the presence of a cancer in a subject and assessing the efficacy of treatments for the same. The disclosed method use reverse transcription polymerase chain reaction (RT-PCR) and multiplex polymerase chain reaction techniques as well as Template Exchange Extension Reaction (TEER) to detect the presence of point mutations, truncations, or fusions of anaplastic lymphoma kinase.

The first hearing of a state commission to implement a public campaign finance system for New York’s elections was overshadowed by the issue of whether to end fusion voting, which allows candidates to run on multiple ballot lines. Critics of the proposal say Governor Cuomo wants to strike against a left leaning party that he’s been feuding with, something the governor denies.

Erica Synths is one of the few manufacturers that uses vacuum tubes in its Eurorack modules — and those who are familiar with tube-based modules know that tubes can push the sound to extremes, or transform it into deep and saturated tones. Three new Fusion series modules have been announced: Fusion VCF3
, Fusion Modulator 
and […]

The post Erica Synths announces new Fusion series modules and system appeared first on rekkerd.org.

Thursday on Political Rewind , the governor says his decision to reopen the state is driven by data. What will be the consequences of Georgians going out and attempting to resume life as normal? We’ll ask two public health experts for their opinions.

If you're designing metal parts, you'll need to do things differently than you would for other materials and processes. This course shows how to use Autodesk Fusion 360—the affordable cloud-powered CAD and CAM tool that works like more expensive, industry-focused tools—to build effective designs specifically for subtractive manufacturing in metal. Thom Tremblay shows how to build solid designs, simulate how the model will hold up to the stress of production and use, render the design for presentation, and finalize it for manufacturing. The workflows showcased in this course are tailored to help the nonprofessional CAD user, and the maker, to be more effective and quickly have access to the tools that a few years ago were only available to the few and at an astronomical price.

Plastic has unique properties not shared by other materials. If you're designing something that will eventually be created in plastic, considering the complexities of manufacturing in your design process is critical. In this course, learn how to design parts for additive manufacturing in plastic using Autodesk Fusion 360—the affordable cloud-powered CAD and CAM tool that works like more expensive, industry-focused tools. Instructor Thom Tremblay explains how to create a form, complete and visualize your design, and set it up for 3D printing, all while designing a ready-to-manufacture part. Plus, learn a more complex alternative modeling processes for creating a solid from surfaces and subdividing a solid model.

Rising British rapper, Macca Wiles releases an electrifying new single "Outlined" - a collage of post-'90s grunge rock with modern trap.  Produced by Matt Schwartz (Yungblud, M.O., Massive Attack, Halsey) "Outline" brilliantly fuses the manic alt-rock energy of Nirvana with the trap swagger of Migos. The informal verses guide the listener through a vivid storytelling […]

The post Macca Wiles' fusion of trap and grunge is "Outlined" in new single [Video] appeared first on EARMILK.

Lily was in a dilemma. After discovering the Sabbath truth while listening to Pastor Doug on Bible Answers Live, she approached her work supervisor to request Saturdays off. He flatly refused. But through the prayers of giving people like you, God performed a miracle. Thank you for changing Lily’s life!

Gov. Jay Inslee this week continued lifting restrictions included in his stay-home order meant to slow the spread of the new coronavirus, part of phase one in his four-phase plan to reopen the state. Some outdoor recreation opened earlier this week.

Aussies are finally looking forward to a return to normality after Prime Minister Scott Morrison unveiled a three-step plan to a COVIDSafe Australia yesterday afternoon.

A hospital chartered a plane to get Sam Voss, four, to Brisbane for treatment. But he needs the infusion fortnightly to keep his motor skills.

The hope of nuclear fusion is the dream of a fossil-fuel free future - of limitless baseload power. Enthusiasts say fusion offers all the benefits of nuclear energy without the dangers. In theory and in practice fusion energy is already a reality, but getting the economics right is proving much more difficult than imagined.

A Victorian-New South Wales cross-border community is uncertain when students should return to the classroom, as term two gets underway.

It's been called a bribe and a wedge — a $3 billion cheque for Catholic and independent schools to ignore the edict from the Andrews Government to teach kids from home, writes Richard Willingham.

(United States Seventh Circuit) - In an insolvent corporation's suit against defendant-corporation for tortious interference with its merger agreement, district court's judgment awarding defendant about $1.2 million in legal fees after ruling in favor of defendant and holding the insolvent corporation's board of directors personally liable is reversed as, under Nev. Rev. Stat. section 78.747, there isn't any fraud as plaintiff's thin capitalization was both the reason why the deal had been proposed and the dominant feature in the deal's structure. Furthermore, when plaintiff signed a contract promising to reimburse defendant's legal expenses if litigation ensued, defendant knew beyond doubt that plaintiff would be unable to keep that promise unless the merger closed. Thus, the court is not aware of any statute or decision holding that investors in a thinly capitalized corporation are personally liable for its debts to a contracting partner when that partner, with knowledge of the corporation's insolvency, signs without getting a guaranty from the investors.

BACKGROUND Preclinical experiments have shown that donor blood cells, modified in vitro by an alkylating agent (modified immune cells [MICs]), induced long-term specific immunosuppression against the allogeneic donor.METHODS In this phase I trial, patients received either 1.5 × 106 MICs per kg BW on day –2 (n = 3, group A), or 1.5 × 108 MICs per kg BW on day –2 (n = 3, group B) or day –7 (n = 4, group C) before living donor kidney transplantation in addition to post-transplantation immunosuppression. The primary outcome measure was the frequency of adverse events (AEs) until day 30 (study phase) with follow-up out to day 360.RESULTS MIC infusions were extremely well tolerated. During the study phase, 10 treated patients experienced a total of 69 AEs that were unlikely to be related or not related to MIC infusion. No donor-specific human leukocyte antigen Abs or rejection episodes were noted, even though the patients received up to 1.3 × 1010 donor mononuclear cells before transplantation. Group C patients with low immunosuppression during follow-up showed no in vitro reactivity against stimulatory donor blood cells on day 360, whereas reactivity against third-party cells was still preserved. Frequencies of CD19+CD24hiCD38hi transitional B lymphocytes (Bregs) increased from a median of 6% before MIC infusion to 20% on day 180, which was 19- and 68-fold higher, respectively, than in 2 independent cohorts of transplanted controls. The majority of Bregs produced the immunosuppressive cytokine IL-10. MIC-treated patients showed the Immune Tolerance Network operational tolerance signature.CONCLUSION MIC administration was safe and could be a future tool for the targeted induction of tolerogenic Bregs.TRIAL REGISTRATION EudraCT number: 2014-002086-30; ClinicalTrials.gov identifier: NCT02560220FUNDING Federal Ministry for Economic Affairs and Technology, Berlin, Germany, and TolerogenixX GmbH, Heidelberg, Germany.

BACKGROUND Glucose-6-phosphate dehydrogenase (G6PD) deficiency decreases the ability of red blood cells (RBCs) to withstand oxidative stress. Refrigerated storage of RBCs induces oxidative stress. We hypothesized that G6PD-deficient donor RBCs would have inferior storage quality for transfusion as compared with G6PD-normal RBCs.METHODS Male volunteers were screened for G6PD deficiency; 27 control and 10 G6PD-deficient volunteers each donated 1 RBC unit. After 42 days of refrigerated storage, autologous 51-chromium 24-hour posttransfusion RBC recovery (PTR) studies were performed. Metabolomics analyses of these RBC units were also performed.RESULTS The mean 24-hour PTR for G6PD-deficient subjects was 78.5% ± 8.4% (mean ± SD), which was significantly lower than that for G6PD-normal RBCs (85.3% ± 3.2%; P = 0.0009). None of the G6PD-normal volunteers (0/27) and 3 G6PD-deficient volunteers (3/10) had PTR results below 75%, a key FDA acceptability criterion for stored donor RBCs. As expected, fresh G6PD-deficient RBCs demonstrated defects in the oxidative phase of the pentose phosphate pathway. During refrigerated storage, G6PD-deficient RBCs demonstrated increased glycolysis, impaired glutathione homeostasis, and increased purine oxidation, as compared with G6PD-normal RBCs. In addition, there were significant correlations between PTR and specific metabolites in these pathways.CONCLUSION Based on current FDA criteria, RBCs from G6PD-deficient donors would not meet the requirements for storage quality. Metabolomics assessment identified markers of PTR and G6PD deficiency (e.g., pyruvate/lactate ratios), along with potential compensatory pathways that could be leveraged to ameliorate the metabolic needs of G6PD-deficient RBCs.TRIAL REGISTRATION ClinicalTrials.gov NCT04081272.FUNDING The Harold Amos Medical Faculty Development Program, Robert Wood Johnson Foundation grant 71590, the National Blood Foundation, NIH grant UL1 TR000040, the Webb-Waring Early Career Award 2017 by the Boettcher Foundation, and National Heart, Lung, and Blood Institute grants R01HL14644 and R01HL148151.

IBM today announced that Vancouver-based company Fusion Genomics selected IBM’s cloud capabilities to help advance Fusion’s ability to detect pandemics before they happen. Fusion Genomics has developed disruptive DNA and RNA technology that it believes can positively identify infectious diseases and enhance the surveillance of emerging pathogens like MERS, SARS, avian flu, and swine flu.

Confusion and frustration bedevil attempts to keep employees on the job while getting a Payroll Protection Program loan.

SIMON COWELL, 60, and his partner, Lauren Silverman, live both in the UK and the US splitting their time. So what are their homes like?

The City of Ottawa is launching a “Parks Ambassador Program” to educate residents on the do’s and don’ts in parks during the COVID-19 pandemic.

Ulrich Rothbauer
Feb 1, 2008; 7:282-289
Research

N. N. Leonenko, I. Papić, A. Sikorskii and N. Šuvak
Theor. Probability and Math. Statist. 99 (2020), 137-147.
Abstract, references and article information

Janet L. Kelly
Aug 1, 2014; 27:218-223
Pharmacy and Therapeutics

(American Society of Agronomy) Researchers develop soil-gas diffusivity model based on two agricultural soils.

⁶⁸Ga-DOTATOC-PET/MRI (⁶⁸Gallium-DOTATOC-positron emission tomography/magnetic resonance imaging) combines the advantages of PET in the acquisition of metabolic-functional information with the high soft tissue contrast of MRI. Standardized uptake values (SUV) in tumors were suggested as a measure of somatostatin receptor expression. A challenge with receptor ligands is, that the distribution volume is confined to tissues with tracer-uptake, potentially limiting SUV quantification. In this study, different functional, three-dimensional (3D) SUV, apparent diffusion coefficient (ADC) parameters and arterial tumor enhancement were tested for the characterization of gastroendopancreatic neuroendocrine tumors (GEP-NET). Methods: For this single-center, cross-sectional study, 22 patients with 24 histologically confirmed GEP-NET lesions (15 men/7 women; median, 61 years, range, 43-81 years), who received hybrid ⁶⁸Ga-DOTA-PET/MRI examinations at 3T between January 2017 and July 2019 met eligibility criteria. SUVs, tumor-to-background ratios (TBR), the total functional tumor volume (TFTV), ADCmean and ADCmin were measured based on volumes of interest (VOI) and examined with receiver operating characteristic analysis to determine cut-off values for differentiation between low and intermediate grade GEP-NET. Spearman’s rank correlation coefficients were used to assess correlations between functional imaging parameters. Results: The ratio of PET-derived SUVmean and diffusion-weighted imaging (DWI)-derived ADCmin was introduced as a combined variable to predict tumor grade, outperforming single predictors. Based on a threshold ratio of 0.03 to be exceeded, tumors could be classified as grade 2 with a sensitivity of 86% and specificity of 100%. SUV and functional ADC values as well as arterial contrast enhancement parameters showed non-significant and mostly negligible correlations. Conclusion: As receptor density and tumor cellularity appear to be independent, potentially complementary phenomena, the combined PET/MRI ratio SUVmean/ADCmin may be used as a novel biomarker, allowing to differentiate between grade 1 and 2 GEP-NET.

The purpose of this study was to investigate the feasibility and diagnostic efficacy of ⁶⁸Ga-PSMA positron emission tomography/computed tomography (PET/CT) combined with PET-ultrasound image-guided biopsy in the diagnosis of prostate cancer. Methods: A total of 31 patients with previously negative prostate biopsy, but persistent elevated serum prostate specific antigen (PSA), were imaged with a ⁶⁸Ga-labeled prostate-specific membrane antigen (PSMA) PET/CT ligand prior to undergoing repeat prostate biopsy. Based on the proposed PROMISE criteria, PSMA PET/CT results were interpreted as negative (miPSMA-ES 0-1) or positive (miPSMA-ES 2-3). All patients underwent standard template systematic biopsy with up to four additional PSMA PET-ultrasound fusion image-guided biopsy cores. The sensitivity, specificity, positive and negative predictive values, and accuracy of PSMA PET/CT were determined. In addition, the correlation between miPSMA-ES and detection rate of prostate cancer was also analyzed. Univariate logistic regression models were established using PSMA PET/CT semi-quantitative analysis parameters to predict the outcome of repeat prostate biopsy. Results: The median age of patients was 65 years (range 53-81), and the median PSA level was 18.0 ng/ml (range 5.48-49.77 ng/ml). Prostate cancer was detected in 15/31 patients (48.4%) and 12/31 patients (38.7%) had clinically significant disease. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of ⁶⁸Ga-PSMA PET/CT in the diagnosis of clinically significant prostate cancer were 100.0%, 68.4%, 66.7%, 100.0% and 80.6%, respectively. The detection rate of prostate cancer increased with the increase of miPSMA-ES score. The detection rate of clinically significant prostate cancer in miPSMA-ES 0-1, 2 and 3 groups were 0%, 54.5% and 85.7% respectively. Semi-quantitative analysis of ⁶⁸Ga-PSMA PET/CT images showed that predictive models based on maximum standardized uptake value (SUV_max), tumor-to-background normal prostate SUV (SUVT/BGp) and tumor-to-background normal liver SUV (SUVratio) could effectively predict clinically significant prostate cancer; area under the curves were 0.930, 0.877, and 0.956, respectively. Conclusion: This study preliminarily confirmed that ⁶⁸Ga-PSMA PET/CT imaging combined with PET-ultrasound fusion image-guided prostate biopsy can effectively detect clinically significant prostate cancer. Prebiopsy ⁶⁸Ga-PSMA PET/CT has predictive value for clinically significant cancer in the studied patient population.

Objectives: The study compared the diagnostic performance of Planar Ventilation/perfusion (V/Q) and V/Q Single-photon computed tomography (SPECT), and determined whether combining perfusion scanning with low-dose computed tomography (Q-LDCT) may be equally effective in a prospective study of patients with chronic thromboembolic pulmonary hypertension (CTEPH) patients. Background: V/Q scanning is recommended for excluding CTEPH during the diagnosis of pulmonary hypertension (PH). However, Planar V/Q and V/Q SPECT techniques have yet to be compared in patients with CTEPH. Methods: Patients with suspected PH were eligible for the study. PH attributable to left heart disease or lung disease was excluded, and patients whose PH was confirmed by right heart catheterization and who completed Planar V/Q, V/Q-SPECT, Q-LDCT, and pulmonary angiography were included. V/Q images were interpreted and patients were diagnosed as instructed by the 2009 EANM guidelines, and pulmonary angiography analyses were used as a reference standard. Results: A total of 208 patients completed the study, including 69 with CTEPH confirmed by pulmonary angiography. Planar V/Q, V/Q-SPECT, and Q-LDCT were all highly effective for diagnosing CTEPH, with no significant differences in sensitivity or specificity observed among the three techniques (Planar V/Q [sensitivity/specificity]: 94.20%/92.81%; V/Q-SPECT: 97.10%/91.37%, Q-LCDT: 95.65%/90.65%). However, V/Q-SPECT was significantly more sensitive (V/Q-SPECT: 79.21%; Planar V/Q: 75.84%, P = 0.012; Q-LDCT: 74.91%, p<0.001), and Planar V/Q was significantly more specific (Planar V/Q: 54.14%; V/Q-SPECT 46.05%, p<0.001; Q-LDCT: 46.05%, P = 0.001) than the other two techniques for identifying perfusion defects in individual lung segments. Conclusion: Both Planar V/Q and V/Q-SPECT were highly effective for diagnosing CTEPH, and Q-LDCT may be a reliable alternative method for patients who are unsuitable for ventilation imaging.

apoB exists as apoB100 and apoB48, which are mainly found in hepatic VLDLs and intestinal chylomicrons, respectively. Elevated plasma levels of apoB-containing lipoproteins (Blps) contribute to coronary artery disease, diabetes, and other cardiometabolic conditions. Studying the mechanisms that drive the assembly, intracellular trafficking, secretion, and function of Blps remains challenging. Our understanding of the intracellular and intraorganism trafficking of Blps can be greatly enhanced, however, with the availability of fusion proteins that can help visualize Blp transport within cells and between tissues. We designed three plasmids expressing human apoB fluorescent fusion proteins: apoB48-GFP, apoB100-GFP, and apoB48-mCherry. In Cos-7 cells, transiently expressed fluorescent apoB proteins colocalized with calnexin and were only secreted if cells were cotransfected with microsomal triglyceride transfer protein. The secreted apoB-fusion proteins retained the fluorescent protein and were secreted as lipoproteins with flotation densities similar to plasma HDL and LDL. In a rat hepatoma McA-RH7777 cell line, the human apoB100 fusion protein was secreted as VLDL- and LDL-sized particles, and the apoB48 fusion proteins were secreted as LDL- and HDL-sized particles. To monitor lipoprotein trafficking in vivo, the apoB48-mCherry construct was transiently expressed in zebrafish larvae and was detected throughout the liver. These experiments show that the addition of fluorescent proteins to the C terminus of apoB does not disrupt their assembly, localization, secretion, or endocytosis. The availability of fluorescently labeled apoB proteins will facilitate the exploration of the assembly, degradation, and transport of Blps and help to identify novel compounds that interfere with these processes via high-throughput screening.