Environmental News FOR IMMEDIATE RELEASE

Environmental News FOR IMMEDIATE RELEASE

DENVER (April 23, 2020)) - Today, the U.S. Environmental Protection Agency (EPA) recognized Sprouts Farmers Market Store #305 in Aurora, CO and Sprouts Farmers Market Store #325 in Englewood, CO, with 2019 Food Recovery Challenge Regional Awards.

Environmental News FOR IMMEDIATE RELEASE

Vincent Edwards signed with the Houston Rockets on Thursday, per the team.

Long-time Slashdot reader blackbearnh writes: Since the COVID-19 pandemic has made Personal Protective Equipment worth it's weight in gold, Makers have been trying to help bridge the gap. While sewn masks have been the most common solution, the 3D printing community has been pitching in as well. The Montana Mask has been one of the most popular designs... Thursday, the group Make the Masks announced that the design files and STLs to print the mask have been released under the GNU General Public License v3, allowing anyone to print, sell, remix or improve the design, as long as they conform to the license. Importantly, the GPLv3 includes an international non-exclusive patent grant, meaning that even if the inventors decide to apply for a patent, it will not restrict anyone from using the design.

Read more of this story at Slashdot.

Borussia Dortmund star Erling Braut Haaland has been backed to sign for Liverpool in the future, while Manchester United have been criticised for missing out on him.

Although a robust inflammatory response is needed to combat infection, this response must ultimately be terminated to prevent chronic inflammation. One mechanism that terminates inflammatory signaling is the production of alternative mRNA splice forms in the Toll-like receptor (TLR) signaling pathway. Whereas most genes in the TLR pathway encode positive mediators of inflammatory signaling, several, including that encoding the MyD88 signaling adaptor, also produce alternative spliced mRNA isoforms that encode dominant-negative inhibitors of the response. Production of these negatively acting alternatively spliced isoforms is induced by stimulation with the TLR4 agonist lipopolysaccharide (LPS); thus, this alternative pre-mRNA splicing represents a negative feedback loop that terminates TLR signaling and prevents chronic inflammation. In the current study, we investigated the mechanisms regulating the LPS-induced alternative pre-mRNA splicing of the MyD88 transcript in murine macrophages. We found that 1) the induction of the alternatively spliced MyD88 form is due to alternative pre-mRNA splicing and not caused by another RNA regulatory mechanism, 2) MyD88 splicing is regulated by both the MyD88- and TRIF-dependent arms of the TLR signaling pathway, 3) MyD88 splicing is regulated by the NF-κB transcription factor, and 4) NF-κB likely regulates MyD88 alternative pre-mRNA splicing per se rather than regulating splicing indirectly by altering MyD88 transcription. We conclude that alternative splicing of MyD88 may provide a sensitive mechanism that ensures robust termination of inflammation for tissue repair and restoration of normal tissue homeostasis once an infection is controlled.

Protease-activated receptor 2 (PAR-2) is activated by secreted proteases from immune cells or fungi. PAR-2 is normally expressed basolaterally in differentiated nasal ciliated cells. We hypothesized that epithelial remodeling during diseases characterized by cilial loss and squamous metaplasia may alter PAR-2 polarization. Here, using a fluorescent arrestin assay, we confirmed that the common fungal airway pathogen Aspergillus fumigatus activates heterologously-expressed PAR-2. Endogenous PAR-2 activation in submerged airway RPMI 2650 or NCI–H520 squamous cells increased intracellular calcium levels and granulocyte macrophage–colony-stimulating factor, tumor necrosis factor α, and interleukin (IL)-6 secretion. RPMI 2650 cells cultured at an air–liquid interface (ALI) responded to apically or basolaterally applied PAR-2 agonists. However, well-differentiated primary nasal epithelial ALIs responded only to basolateral PAR-2 stimulation, indicated by calcium elevation, increased cilia beat frequency, and increased fluid and cytokine secretion. We exposed primary cells to disease-related modifiers that alter epithelial morphology, including IL-13, cigarette smoke condensate, and retinoic acid deficiency, at concentrations and times that altered epithelial morphology without causing breakdown of the epithelial barrier to model early disease states. These altered primary cultures responded to both apical and basolateral PAR-2 stimulation. Imaging nasal polyps and control middle turbinate explants, we found that nasal polyps, but not turbinates, exhibit apical calcium responses to PAR-2 stimulation. However, isolated ciliated cells from both polyps and turbinates maintained basolateral PAR-2 polarization, suggesting that the calcium responses originated from nonciliated cells. Altered PAR-2 polarization in disease-remodeled epithelia may enhance apical responses and increase sensitivity to inhaled proteases.

Endorepellin, the C-terminal fragment of the heparan sulfate proteoglycan perlecan, influences various signaling pathways in endothelial cells by binding to VEGFR2. In this study, we discovered that soluble endorepellin activates the canonical stress signaling pathway consisting of PERK, eIF2α, ATF4, and GADD45α. Specifically, endorepellin evoked transient activation of VEGFR2, which, in turn, phosphorylated PERK at Thr980. Subsequently, PERK phosphorylated eIF2α at Ser51, upregulating its downstream effector proteins ATF4 and GADD45α. RNAi-mediated knockdown of PERK or eIF2α abrogated the endorepellin-mediated up-regulation of GADD45α, the ultimate effector protein of this stress signaling cascade. To functionally validate these findings, we utilized an ex vivo model of angiogenesis. Exposure of the aortic rings embedded in 3D fibrillar collagen to recombinant endorepellin for 2–4 h activated PERK and induced GADD45α vis à vis vehicle-treated counterparts. Similar effects were obtained with the established cellular stress inducer tunicamycin. Notably, chronic exposure of aortic rings to endorepellin for 7–9 days markedly suppressed vessel sprouting, an angiostatic effect that was rescued by blocking PERK kinase activity. Our findings unravel a mechanism by which an extracellular matrix protein evokes stress signaling in endothelial cells, which leads to angiostasis.

State-of-the-art electron microscopy reveals the large-scale organization of the proteins that regulate neurotransmitter release

This spectacular image – which took the best part of a year to create – shows the fine structure of a nerve terminal at high resolution, revealing, for the very first time, an intricate network of fine filaments that controls the movements of synaptic vesicles.

The brain is soft and wet, with the consistency of a lump of jelly. Yet, it is the most complex and highly organized structure that we know of, containing hundreds of billions of neurons and glial cells, and something on the order of one quadrillion synaptic connections, all of which are arranged in a very specific manner.

Related: 3D model of a nerve terminal in atomic detail | Mo Costandi

Related: Blowing up the brain to reveal its finer details

Testimony of Marc R. Rosenblum, Deputy Director, U.S. Immigration Program, before the House Committee on Homeland Security Subcommittee on Oversight and Management Efficiency.

A pastor of a small community church was about to resign when God brought new hope through the work of OM El Salvador.

In addition to the prizes and rewards, Hero MotoCorp may also make it a memorable experience for the participants by putting some of the entries into production.

The Benda Asura 400 comes with a single-sided swingarm, which is quite rare to see on a 400cc motorcycle.

The Audi A5 is one of the best looking and also one of the best driving cars money can buy in India. And here’s why!

The decrease in WiFi usage can also be attributed to people staying at homes.

Airtel emerged on the top in terms of latency experience with an average response time of 54.1 milliseconds, followed by Jio at 56.3 ms.

Transfer amounts should also be linked to CPI. While PM-KISAN covers only farmers and is much more expensive and exclusive, such a transfer would avoid narrow coverage, also avoiding inclusion/exclusion errors

The basic decision making on the ground must be left to the states and should involve municipal corporations and wards.

The scientists tested their vaccine on mice. The mice developed antibodies immediately after a single injection, which prevented the virus from infecting human cells. Researchers selected 2 of the five candidates for the vaccine after studying them with the best results.

Hong Kong and Federal Republic of Germany have signed a Memorandum of Understanding (MOU) concerned with the automated clearance service usage on a shared basis. The MOU is considered as a new milestone in the collaboration between the countries in immigration…

The brand has released a new television commercial featuring the actor

Delhi recorded the maximum temperature of 40.9 degrees centigrade on Saturday, the IMD said. Due to the Western Disturbance, the IMD in its weather summary and forecast bulletin predicted isolated to scattered rain over the Western Himalayan region and plains of Northwest India, with peak activity on May 10, 2020.

Cadence is hosting a Front End Design Summit on Thursday, December 6, 2012 9:30am – 5:00pm at Cadence San Jose headquarters, 2655 Seely Avenue, Building 10. Logic designers will hear from customers including Cisco, Chelsio, PMC, Spansion, and Via Technologies...(read more)

Anomalous Payloadbased Worm Detection and Signature Generation.

Business leaders have an important decision to make this year: to continue operating under the status quo or to join the list of successful companies creating a more sustainable future by contracting or investing in renewable energy and making a positive impact on their brand, customers, employees and bottom line.

Coal is having a hard time lately. U.S. power plants are switching to natural gas, environmental restrictions are kicking in, and the industry is being derided as the world's No. 1 climate criminal. Prices have crashed, sure, but for a real sense of coal's diminishing prospects, check out what's happening in the bond market.

The G20 meeting in Hangzhou, China, this September brings together leaders of the world’s largest economies for the first such gathering since the 2015 Paris Agreement on climate. G20 Leaders Summits traditionally focus on economic growth and financial stability, but since more than 190 countries collectively agreed to greatly enhance mitigation of the causes and impacts of climate change, the need to tackle a changing climate and foster clean energy has become a clear economic and business reality.

Having inherited the Agency Workers Regulations 2010 on taking office, the UK Government had pledged to review them. That process is now underway but it seems the EU may pip the Government to the post, having conducted its own review of the parent D...

Precis Having inherited the Agency Workers Regulations 2010 on taking office, the UK Government had pledged to review them. That process is now underway but it seems the EU may pip the Government to the post, having conducted its own review of the ...