Teacher question: Everyone says reading and writing are connected. But our school focuses on only reading. We have a reading program (we don’t have a writing program). We test the students three times a year in reading, but never in writing. Writing isn’t even on our report card, though I guess it is part of Language Arts. What should we be doing with writing? Shanahan's response: You came to the right place. I think your school is making a big mistake not giving sufficient attention to writing.

Many often consider negotiation an arduous task, as it involves having conversations with people, where both parties are doing their best to protect personal interests. Going over negotiations while maintaining relationships remains relevant to businesses and investments. It hardly comes easy to conclude a negotiation that keeps both parties happy, while overlooking small processes may […]

The post 7 Common Negotiation Mistakes to Avoid at all Costs appeared first on ReadWrite.

In the past, we’ve all witnessed sim-jacking attacks that allow a hacker to impersonate the targeted victim to steal the phone number. Hackers can gain access to unauthorized information related to the victim using the vulnerability, ‘SimJacker’ was that vulnerability. Recently there’s a similar vulnerability that has popped up, which uses the same SMS-technology to […]

The post WIB Vulnerability: Sim-Card that Allows Hackers to Takeover Phones appeared first on ReadWrite.

You have to invest real time into earning links from high-authority sites by doing your own research and digital public relations.

Please visit Search Engine Land for the full article.

Warner Bros. systematically abused an automated takedown system provided by Hotfile, the file host claims in a countersuit against the studio. The claims made by Hotfile include information on the behind-the-scenes actions taken by Hollywood to enforce its rights against file sharing on cloud file hosts. Continue reading on NewTeeVee.

After Mmusi Maimane's resignation as DA leader, Buccus recycled the madcap mutterings of Herman Mashaba, warning that the DA had been captured by the "right-wing zealots of the Institute of Race Relations".

The 2Checkout team kicked off Feb 2019 by joining over 12,000 participants in San Jose for SaaStr Annual 2019, the largest conference in the world for B2B software. The general consensus of 2Checkout team members at the end of the conference was simple and to the point: “It. Was. Amazing.”

Do you have the equivalent of a will for all your digital possessions—photos, email, online accounts, and all the rest? If not, you should, and we have a book that shows you what to do. Plus, Interesting Thing of the Day readers can save 30% on it.

Digital cameras make it easy to take way too many pictures. Need help sorting, organizing, storing, and managing them? We've got a great book for you—and Interesting Thing of the Day readers can save 30% on it.

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If Instagram is to be believed, then self care for women basically amounts to face masks and daily affirmation apps.

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Orgasms release oxytocin, the anti-stress hormone that gets you warm and fuzzy when cuddling, while dopamine, a neurotransmitter that regulates sleep, also makes an appearance. It stands to reason, then, that "I need to get laid" is a thing that actually makes sense to say when you're having a rough week. Read more...

A Sharp Intake Of Breath Synopsis A Sharp Intake Of Breath was a situation comedy television series produced by ITV that premiered in February of 1978. Created by Ronnie Taylor A Sharp Intake Of Breath followed the story of Peter Barnes (played by David Jason – Only Fools And Horses), an ordinary man who made […]

Travel is fun, when you plan things properly. If you have visited many countries, then surely you have made many mistakes such as booking resorts at wrong places or buying costly souvenirs which are easily available in your country. We all make silly mistakes when we visit a new country and there is no need to feel ashamed. What you need to do is make sure that you don’t repeat those mistakes.

When it comes to mistakes, I am not talking about flight or hotel ticket booking blunders. There are many more small oversights that can ruin your vacation. Let’s explore some of the common mistakes that tourists make every year.

No research about the place: You may know the name of the country, its capital and its currency exchange rate, but do you really know the place you are visiting? For example, in India, there are many prepaid taxi booths that charge right amount for a trip, but if you just pick any cab from the road, the driver may charge you double because you don’t know the route to your destination. Similarly, it is important to know the things you can and can’t do in the place you are visiting. For example, taking pictures is prohibited in many religious places.

Over-packing: Never carry too many clothes, because you are going to carry your bags, and if those are too heavy, you will curse yourself. Besides, the airlines charge hefty amount if your luggage crosses the weight limit. Always pack only those things that are necessary for the place you are visiting. For example, if you are going to Tadoba tiger reserve forest and staying at Tadoba tiger resort, then you don’t need to pack a cocktail party dress. It is better to pack a few jeans and t-shirts.

Speaking English only: Try to learn a few sentences in the local language. For example, words of greetings, how to ask for road directions and how to ask the price of something. The more you communicate with the locals in their own language, the more confident you will feel.

Disrespecting the customs: India is a conservative county, and the culture of Indians is very different from Americans. However, that does not mean you can mock or disrespect their culture. For example, Americans enter churches wearing shoes, but in India, you can’t wear shoes in a temple. Respect the rule while visiting any temple in India. After all, you are visiting a new country to understand its culture.

Reading guidebooks only: Guidebooks are for references and they can’t give you all the information about the place. For example, a guidebook may tell you that Taj Mahal is wonderful, but unless you raise your face from the book, can you truly appreciate its beauty?

Booking hotels and flights separately: Take a look at the famous travel portals and you will find that they are offering great hotel + airplane deals. When you book return tickets and also hotel rooms, you can save a few hundred dollars. Besides, it is a hassle free process as you are getting everything from one site.

Interrupting the guide: You can be the history professor in a college, but if you interrupt the guide when he is talking about a historical place, then you are being rude. All the other tourists have paid for the guided tour and by interrupting the guide frequently you are making everyone angry.

So, when you visit a foreign country this year, make sure that you don’t make the mistakes mentioned above. Enjoy nature, history and culture of the place you are visiting and you will feel rejuvenated by the end of your vacation.

If you like traveling to exotic places, check out our Machu Picchu Tours page

There’s no perfect process for hiring great programmers, but there are plenty of terrible ways to screw it up. We’ve rejected the industry stables of grilling candidates in front of a whiteboard or needling them with brain teasers since the start at Basecamp. But you’re not getting around showing real code when applying for a… keep reading

Part of the Food for thought promo for the BBC UK Homepage

School bus drivers in the Upper Grand District School Board are transporting school work instead of students.

Restaurants have been struggling to stay afloat since the pandemic hit, but there has been a silver lining: relaxed liquor laws mean customers can get their booze delivered along with their meals.

The following article, Video Scrubbed of Obama-Biden Ambassador To China Praising Their Response to Coronavirus: “I take my hat off to China”, was first published on 100PercentFedUp.com.

During a recent interview on CNN, Obama-Biden ambassador to China (2014-2017) Max Baucus compared standing up to China on the coronavirus to “Hitler in the ’30s.” Baucus has proven himself to be sympathetic to China in recent interviews, where he puts down America and praises the Chinese. The MSNBC video via The Washington Free Beacon […]

Continue reading: Video Scrubbed of Obama-Biden Ambassador To China Praising Their Response to Coronavirus: “I take my hat off to China” ...

16 January 2020

The ribosome biogenesis factor Las1 is an essential endoribonuclease that is well-conserved across eukaryotes and a newly established member of the higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domain-containing nuclease family. HEPN nucleases participate in diverse RNA cleavage pathways and share a short HEPN nuclease motif (RφXXXH) important for RNA cleavage. Most HEPN nucleases participate in stress-activated RNA cleavage pathways; Las1 plays a fundamental role in processing pre-rRNA. Underscoring the significance of Las1 function in the cell, mutations in the human LAS1L (LAS1-like) gene have been associated with neurological dysfunction. Two juxtaposed HEPN nuclease motifs create Las1's composite nuclease active site, but the roles of the individual HEPN motif residues are poorly defined. Here using a combination of in vivo experiments in Saccharomyces cerevisiae and in vitro assays, we show that both HEPN nuclease motifs are required for Las1 nuclease activity and fidelity. Through in-depth sequence analysis and systematic mutagenesis, we determined the consensus HEPN motif in the Las1 subfamily and uncovered its canonical and specialized elements. Using reconstituted Las1 HEPN-HEPN' chimeras, we defined the molecular requirements for RNA cleavage. Intriguingly, both copies of the Las1 HEPN motif were important for nuclease function, revealing that both HEPN motifs participate in coordinating the RNA within the Las1 active site. We also established that conformational flexibility of the two HEPN domains is important for proper nuclease function. The results of our work reveal critical information about how dual HEPN domains come together to drive Las1-mediated RNA cleavage.

S Xu
Jul 1, 1997; 38:1289-1298
Articles

The ribosome biogenesis factor Las1 is an essential endoribonuclease that is well-conserved across eukaryotes and a newly established member of the higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domain-containing nuclease family. HEPN nucleases participate in diverse RNA cleavage pathways and share a short HEPN nuclease motif (RφXXXH) important for RNA cleavage. Most HEPN nucleases participate in stress-activated RNA cleavage pathways; Las1 plays a fundamental role in processing pre-rRNA. Underscoring the significance of Las1 function in the cell, mutations in the human LAS1L (LAS1-like) gene have been associated with neurological dysfunction. Two juxtaposed HEPN nuclease motifs create Las1's composite nuclease active site, but the roles of the individual HEPN motif residues are poorly defined. Here using a combination of in vivo experiments in Saccharomyces cerevisiae and in vitro assays, we show that both HEPN nuclease motifs are required for Las1 nuclease activity and fidelity. Through in-depth sequence analysis and systematic mutagenesis, we determined the consensus HEPN motif in the Las1 subfamily and uncovered its canonical and specialized elements. Using reconstituted Las1 HEPN-HEPN' chimeras, we defined the molecular requirements for RNA cleavage. Intriguingly, both copies of the Las1 HEPN motif were important for nuclease function, revealing that both HEPN motifs participate in coordinating the RNA within the Las1 active site. We also established that conformational flexibility of the two HEPN domains is important for proper nuclease function. The results of our work reveal critical information about how dual HEPN domains come together to drive Las1-mediated RNA cleavage.

euromicron AG, a medium-sized technology group and specialist for networking business and production processes, has taken on Funkwerk AG, Kölleda, as a strategic anchor investor. Funkwerk AG is a leading provider of innovative communication, information and security systems and intends to acquire a total of up to around 28% of euromicron AG’s increased share capital following the implementation of the resolved capital measures.

The office space company has filed for an initial public offering, here are five things you need to know about one of the year's hottest IPOs

The digital challenger bank is now the most switched to bank in the UK as Nationwide Building Society loses its long-held top spot

The ribosome biogenesis factor Las1 is an essential endoribonuclease that is well-conserved across eukaryotes and a newly established member of the higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domain-containing nuclease family. HEPN nucleases participate in diverse RNA cleavage pathways and share a short HEPN nuclease motif (RφXXXH) important for RNA cleavage. Most HEPN nucleases participate in stress-activated RNA cleavage pathways; Las1 plays a fundamental role in processing pre-rRNA. Underscoring the significance of Las1 function in the cell, mutations in the human LAS1L (LAS1-like) gene have been associated with neurological dysfunction. Two juxtaposed HEPN nuclease motifs create Las1's composite nuclease active site, but the roles of the individual HEPN motif residues are poorly defined. Here using a combination of in vivo experiments in Saccharomyces cerevisiae and in vitro assays, we show that both HEPN nuclease motifs are required for Las1 nuclease activity and fidelity. Through in-depth sequence analysis and systematic mutagenesis, we determined the consensus HEPN motif in the Las1 subfamily and uncovered its canonical and specialized elements. Using reconstituted Las1 HEPN-HEPN' chimeras, we defined the molecular requirements for RNA cleavage. Intriguingly, both copies of the Las1 HEPN motif were important for nuclease function, revealing that both HEPN motifs participate in coordinating the RNA within the Las1 active site. We also established that conformational flexibility of the two HEPN domains is important for proper nuclease function. The results of our work reveal critical information about how dual HEPN domains come together to drive Las1-mediated RNA cleavage.