highlight OSCE Office in Tajikistan’s training series highlights role of parents in the fight against terrorism By feeds.osce.org Published On :: Tue, 26 Jul 2016 12:27:45 +0000 DUSHANBE, 26 July 2016 – The Parents Against Terrorism training series, organized by the OSCE Office in Tajikistan, started yesterday in Kulyab in the country’s Khatlon region with the aim of enhancing the knowledge and understanding of the local population about Violent Extremism and Radicalization that lead to Terrorism (VERLT) in order to build the community’s resilience to this phenomenon. The trainees came from different layers of society, representing local government bodies, law enforcement agencies, educational institutions, as well as the local population. The course was led by local trainers from civil society, the Ministry of Labour, Migration, and Employment, and the Committee on Religious Affairs. “The government of Tajikistan is seriously concerned about the growing threat of global terrorism and violent extremism,” said Khurshed Kenjaev, a representative of the Security Council under the President, in his opening remarks. “It is taking solid preventative measures to tackle this phenomenon, since it cannot be the sole responsibility of law enforcement agencies to deal with the threat.” Ivan Ene, OSCE Field Officer in Kurgan Tyube, said: “Countering violent extremism and radicalization that may lead to terrorism requires a holistic approach. The intervention of law enforcement agencies to dissolute the problem of violent extremism and terrorism is effective to only a certain extent, therefore, if we want to apply more effective measures to fight this phenomenon, we need to engage and use the capacity of local communities and parents.” The course focuses on the role of mothers as “positive agents of change” within Tajik culture. They are informed about the early signs of violent extremism and radicalization that may lead to terrorism. The training participants were also briefed on the violent extremist groups that are believed to be active in Tajikistan. In the coming days, similar training sessions will be held in Kurgan-Tyube and Shaartuz. A similar activity was organized across Tajikistan in 2015 and recently held in Sughd region. Related StoriesOSCE Office in Tajikistan donates audio-visual material to enhance interactive teaching at Police AcademyOSCE Office in Tajikistan support Garm’s local authorities in strengthening referral mechanisms for protecting domestic violence victimsOSCE Office in Tajikistan organizes workshop on verifying the authenticity of security documents Full Article OSCE Office in Tajikistan Combating terrorism Central Asia Press release
highlight Highlights of 2017 By nanotechweb.org Published On :: 2017-12-18T18:24:57Z From CO2 catalysis based on solar cells to nanoparticle-enhanced antibiotics, we highlight some of the developments that have made the headlines on nanotechweb.org in 2017. (You can also watch as a movie on youtube.) Full Article
highlight 2017 highlights from IOP Materials By nanotechweb.org Published On :: 2017-12-22T12:39:40Z We bring you the highlights nanoscale science and technology research in 2017 from IOP Publishing's materials portfolio. Full Article
highlight Annual Dressage Stakeholder Meeting Highlights Technology-Assisted Judging, Rebuilding Trust - The Chronicle of the Horse By news.google.com Published On :: Tue, 12 Nov 2024 20:25:32 GMT Annual Dressage Stakeholder Meeting Highlights Technology-Assisted Judging, Rebuilding Trust The Chronicle of the Horse Full Article
highlight Spotify Technology SA (SPOT) Q3 2024 Earnings Call Highlights: Record Growth in Users and Revenue - Yahoo Finance By news.google.com Published On :: Wed, 13 Nov 2024 07:22:38 GMT Spotify Technology SA (SPOT) Q3 2024 Earnings Call Highlights: Record Growth in Users and Revenue Yahoo Finance Full Article
highlight PHOTOS: Highlighting Latino Roofers at IRE 2024 By www.roofingcontractor.com Published On :: Wed, 07 Feb 2024 06:48:31 -0500 The number of Latino roofing contractors is on the rise and showed up in force at the 2024 International Roofing Expo. Full Article
highlight Editor's Choice 2020: RC Highlights the Industry’s Latest Innovations and New Products By www.roofingcontractor.com Published On :: Fri, 01 May 2020 00:00:00 -0400 Manufacturers, suppliers and other industry stakeholders are updating their own business practices while learning to navigate through and beyond these unparalleled times. Full Article
highlight Scottish Roofing Accident Highlights Need for Lift Safety Precautions By www.roofingcontractor.com Published On :: Thu, 17 Oct 2024 09:00:00 -0400 A roofing accident across the pond serves as a reminder to take precautions when using lifts, especially when near roads and streets. Full Article
highlight Göteborg Film Festival to Highlight Disobedience: ‘Civil Resistance Has Become Crucial in the Global Conversation’ By variety.com Published On :: Wed, 13 Nov 2024 08:01:40 +0000 In the light of current world events, defiance will be in sharp relief at the 48th Göteborg Film Festival, which runs Jan. 26 – Feb. 4, 2025 Under the banner “Focus: Disobedience,” the festival will highlight themes of civil resistance and pose such questions as: “When does rebellion become a moral obligation? When can it […] Full Article Global Markets & Festivals News Dea Kulumbegashvili Göteborg Film Festival Pia Lundberg
highlight '1600 Penn' highlight clip By weblogs.variety.com Published On :: 2013-03-08T11:42:02-08:00 Full Article
highlight 2024 Sammies highlight extraordinary work of public servants at ‘vital’ time By federalnewsnetwork.com Published On :: Mon, 09 Sep 2024 10:00:12 +0000 The Partnership for Public Service will honor all the 2024 Sammies winners during an awards ceremony on Sept. 11 at the Kennedy Center in Washington, D.C. The post 2024 Sammies highlight extraordinary work of public servants at ‘vital’ time first appeared on Federal News Network. Full Article All News People Workforce Amira Boland Christopher Mark jay evans Jerry Ma Justin Uphold Long Phan Marc Levitan Max Stier Nancy Alcantara Partnership for Public Service Pete Guria Sammies Service to America Medals Shannon Rebolledo Steve Canalog tara fitzgerald Yan Ping Chen
highlight Coast Guard’s new posture statement highlights service’s operational strains By federalnewsnetwork.com Published On :: Fri, 01 Nov 2024 21:25:53 +0000 Coast Guard's new operational posture statement is not a "pitch for money" but rather a realistic assessment of the Coast Guard’s readiness challenges. The post Coast Guard’s new posture statement highlights service’s operational strains first appeared on Federal News Network. Full Article All News Defense Defense News Coast Guard Congress Peter Gautier
highlight ACI Group offers digital solutions as BCI report highlights widespread supply chain chaos By www.logisticsit.com Published On :: ACI Group has shone a spotlight on the importance of supply chain digitalisation in response to a Business Continuity Institute (BCI) report which found that almost 80% of organisations have experienced supply chain disruptions over the past year. Full Article
highlight Adobe MAX 2024: Video highlights of Zach's favourite new gen AI features By www.hardwarezone.com.sg Published On :: Wed, 30 Oct 2024 12:50:34 +0800 Adobe announced over 100 updates across all apps at this year's MAX 2024 conference in Miami, but the most impressive has to be the Firefly Video model. Here are some live demos of Adobe's new gen AI magic at work. #adobe #adobemax More stories from MAX 2024: Adobe Firefly, Adobe Photoshop and Illustrator, Content Authenticity app, Adobe global AI skills initiative Full Article
highlight Apple’s New Benchmark, ‘GSM-Symbolic,’ Highlights AI Reasoning Flaws By circleid.com Published On :: 2024-10-14T10:52:00-07:00 A recent study conducted by Apple's artificial intelligence (AI) researchers has raised significant concerns about the reliability of large language models (LLMs) in mathematical reasoning tasks. Despite the impressive advancements made by models like OpenAI's GPT and Meta's LLaMA, the study reveals fundamental flaws in their ability to handle even basic arithmetic when faced with slight variations in the wording of questions. Full Article
highlight Highlights of Upcoming Week 3 of Citizen Science Month By www.discovermagazine.com Published On :: Fri, 12 Apr 2024 18:00:00 GMT Bats, dolphins, National Parks and a 12-hour Zooniverse Palooza are coming up for Citizen Science Month. Full Article The Sciences
highlight Haslett press conference highlights: Excitement over the rookies By www.washingtonexaminer.com Published On :: Wed, 12 Jun 2013 04:00:00 GMT Redskins defensive coordinator Jim Haslett spoke to the media Wednesday for the first time this offseason. The main topic: the three rookie defensive backs and what to expect at safety. Here are some highlights: Full Article
highlight Newly released numbers highlight the trends behind Washington's recreational cannabis market By www.inlander.com Published On :: Thu, 31 Oct 2024 01:30:00 -0700 The Washington State Liquor and Cannabis Board recently released sales and tax data from fiscal 2024 — July 1, 2023, through June 30, 2024… Full Article Green Zone
highlight Spokane Valley City Council continues debate over Merkel’s actions, and highlights the costs By www.inlander.com Published On :: Tue, 12 Nov 2024 16:22:44 -0800 Last week, Spokane Valley released details about how much the city’s multiple investigations into City Council member Al Merkel have cost. At the Nov. 5 Spokane Valley City Council meeting, an expense report on the agenda stated that two independent investigations found Merkel has displayed a pattern of intimidation and hostile interactions with the city’s workforce, and disregarded the Council’s Governance Manual and Washington state laws despite receiving extensive training on public records rules… Full Article News/Local News
highlight Birmingham Weekender in Digbeth – first programme highlights announcement By thebirminghampress.com Published On :: Thu, 15 Aug 2019 23:01:06 +0000 First participants confirmed through the Birmingham Weekender in Digbeth Open Call. Full Article Dance Digbeth Family Festivals Gaming Music Birmingham Open Media Birmingham Weekender Culture Central Digbeth First Friday
highlight Cannock Chase visit highlights University’s quest to protect forestry By thebirminghampress.com Published On :: Thu, 20 Jun 2013 15:37:21 +0000 A course aiming to assist the international drive to protect the world’s tree population is once again being hosted by the University of Wolverhampton. Full Article Education Farming Most recent Shropshire education environment trees Wolverhampton
highlight University event highlights benefits of short food chains By thebirminghampress.com Published On :: Fri, 05 Jul 2013 08:55:51 +0000 The benefits of short food chains and local produce were the focus of an event on Wednesday 3 July organised by Coventry University’s Centre for Agroecology and Food Security Full Article Coventry Environment Farming Food and drink Most recent Coventry University farming sustainable food
highlight Birmingham Games helps highlight global threat By thebirminghampress.com Published On :: Thu, 18 Aug 2022 20:57:04 +0000 Athletes from across Commonwealth shine light on startling threat of climate change. Full Article Climate Comment Commonwealth Games Environment 2022 Birmingham Commonwealth Games
highlight Activism, Voting Rights, And ‘Good Trouble’: New Film Highlights Legacy Of Congressman John Lewis By www.gpbnews.org Published On :: Fri, 10 Jul 2020 22:32:43 +0000 John Lewis has gotten into a lot of trouble in his life. The now 17-term House Representative from Atlanta has been arrested 45 times – five as a U.S. congressman. One of the original Freedom Riders , Lewis trained in nonviolent resistance, but faced a lot of brutality during his time as a young activist in the civil rights movement. He suffered harassment and attacks during lunch counter sit-ins in Nashville, his skull was fractured by a blow from a Klansman in 1961, and he was badly beaten after crossing the Edmund Pettus Bridge in Selma, Alabama on Bloody Sunday . Full Article
highlight A3C: Highlights From The Live Show By www.gpbnews.org Published On :: Fri, 28 Oct 2016 23:25:26 +0000 For this edition of Atlanta Considered, I went to Chrome Yellow Trading Company on Edgewood Avenue during the A3C hip-hop festival and conference. A3C is one of the nation’s largest urban music events and stands for All Three Coasts, as Atlanta joins New York and Los Angeles as the pillars of hip-hop culture. Full Article
highlight High Museum: Highlights From The Live Show By www.gpbnews.org Published On :: Mon, 13 Feb 2017 17:30:31 +0000 On Friday February 3, GPB Atlanta broadcasted "All Things Considered" live from the High Museum of Art's monthly " First Friday " event. Full Article
highlight Cassidy Hutchinson’s Testimony Highlights Legal Risks for Trump By www.nytimes.com Published On :: Wed, 29 Jun 2022 20:26:07 +0000 The former White House aide’s revelations about Jan. 6 chipped away at any potential defense that Donald J. Trump was merely expressing well-founded views about election fraud. Full Article Hutchinson Cassidy House Select Committee to Investigate the January 6th Attack Presidential Election of 2020 Storming of the US Capitol (Jan 2021) United States Politics and Government Justice Department Trump Donald J
highlight Claude-Michel Schönberg - Les Misérables: Highlights from the Motion Picture Soundtrack By www.bbc.co.uk Published On :: Tue, 15 Jan 2013 00:00:00 +0000 A partial victory, and one buoyed by some outstanding surprise turns. Full Article
highlight Olympian Rebecca Cheptegei dies days after partner set her on fire; officials highlight pattern of 'gender-based violence' By www.yahoo.com Published On :: 2024-09-05T16:56:56Z Full Article
highlight GitHub - dandavison/delta: A syntax-highlighting pager for git By github.com Published On :: 2024-11-13T05:47:01+00:00 jaygooby starred dandavison/delta Full Article
highlight CLI for SQLite Databases with auto-completion and syntax highlighting By github.com Published On :: 2024-11-13T05:47:01+00:00 Full Article
highlight 'I'm a Natural-Born Killer': Mike Tyson Highlights the Difference Between Him and YouTuber Jake Paul By www.breitbart.com Published On :: Tue, 12 Nov 2024 18:00:32 +0000 YouTuber Jake Paul and boxing heavyweight legend Mike Tyson are finally set to battle in their much-anticipated match at AT&T Stadium in Dallas on Friday night. The post ‘I’m a Natural-Born Killer’: Mike Tyson Highlights the Difference Between Him and YouTuber Jake Paul appeared first on Breitbart. Full Article Sports boxing Jake Paul Mike Tyson Netflix
highlight Highlights from the BET Awards 2020 By www.bet.com Published On :: Mon, 6 Jul 2020 17:44:24 EDT A recap of the BET Awards 2020, hosted by Amanda Seales. Full Article BET Awards
highlight Show Highlights By www.bet.com Published On :: Thu, 3 Mar 2016 12:11:00 EST The best moments from a magical evening! Full Article BET Honors
highlight Magazine Highlights The Bermuda Longtail By bernews.com Published On :: Sun, 10 Mar 2024 10:00:13 +0000 Hakai Magazine has featured the iconic Bermuda longtails, noting they have been “turning up in Bermuda earlier and earlier.” The story said, “White-tailed tropicbirds spend most of the year at sea, out of sight of people. When the seabirds make their annual descent into Bermuda to breed, though, their long, streamer-like tails, snowy white plumage, and […] Full Article All Environment #Birds #Longtails
highlight Video: Eater Highlights Lionfish & Fishermen By bernews.com Published On :: Thu, 23 Mar 2023 17:19:10 +0000 The popular food-focused Eater media channel has featured Bermuda fishermen and chefs and their work in the effort to fight the invasive lionfish. The Eater story said,”In the Atlantic Ocean, lionfish are an invasive species that eat many fish in local waters. Bermuda-based fishermen and chefs have found a way to help curb that: “We’ve […] Full Article All Environment News Videos #BermudaMarine #Cuisine #Fishing #Lionfish
highlight Video: 2023 Bermuda Billfish Blast Highlights By bernews.com Published On :: Mon, 14 Aug 2023 12:59:28 +0000 Marlin Magazine has released a video highlighting the 2023 Bermuda Billfish Blast, which is part of the 2023 Bermuda Triple Crown event. The video’s description says, “A total of 37 boats fished the 2023 Bermuda Billfish Blast, which offered a total cash purse of $506,850 plus an additional $360,000 as part of the Bermuda Triple […] Full Article All Videos #BermudaMarine #Fishing
highlight Video: 2024 Big Game Classic Highlights By bernews.com Published On :: Thu, 25 Jul 2024 10:43:10 +0000 Marlin Magazine has released a video highlighting the 2024 Bermuda Big Game Classic, which is part of the 2024 Bermuda Triple Crown event. The video takes a look at both Sea Striker’s win, as well as the 1,268 pound blue marlin caught by UnWined. The video’s description says, “Sea Striker wins the 2024 Bermuda Big […] Full Article All Videos #BermudaMarine #Fishing #Marlin
highlight Video: SailGP Highlights Keeron Wilson By bernews.com Published On :: Mon, 30 Sep 2024 12:45:00 +0000 Bermudian Keeron Wilson’s inspiring journey as a boatbuilder at SailGP Technologies in Southampton, England, has been spotlighted in a recent video. At just 25 years old, Mr Wilson has found success as a full-time technical team member for SailGP, having moved to the UK nearly a year ago. His path into the industry began with […] Full Article All News Sports #BermudaSailing #GoodNews #SailGP
highlight Metro UK Highlights Actor Earl Cameron By bernews.com Published On :: Tue, 15 Oct 2024 15:00:35 +0000 Metro UK has highlighted the late Bermudian actor Earl Cameron in an article titled “My friend Earl Cameron broke the British colour bar in TV and film.” The Metro UK story said, “On a beautiful summer’s day in August 2017, I felt excited and honoured to be among the family and friends celebrating the 100th […] Full Article All Entertainment Films/Movies History #EarlCameron #Film #GoodNews #Theatre
highlight Premier & Family Highlight Local Designer By bernews.com Published On :: Wed, 02 Aug 2023 22:46:16 +0000 Premier David Burt and his family released their annual Cup Match photo, featuring clothing by Bermudian designer, business owner and creative Jordan Carey, founder and Creative Director of the Loquat brand. A spokesperson said, “Premier David Burt, JP, MP and his family have today shared their annual Cup Match photo. “This year’s official photoshoot is particularly […] Full Article All Entertainment News Style & Beauty #CupMatch
highlight BTA Highlights Bermuda Art Month’s Final Week By bernews.com Published On :: Thu, 24 Oct 2024 14:38:51 +0000 As Bermuda’s second annual Art Month draws to a close, the Bermuda Tourism Authority [BTA] “encourages both locals and visitors to take advantage of the ongoing exhibitions and remaining events that have made this celebration of Bermuda’s vibrant culture and creativity such a success.” A spokesperson said, “While October is nearly over, the exhibits at […] Full Article All Entertainment #Artists #BermudaTourism
highlight Streaks and highlights By www.planetary.org Published On :: Fri, 08 Nov 2024 07:30:00 -0800 It’s been a great year for space exploration. Now you get to pick the highlights. Full Article
highlight New photo exhibit at ANU highlights rich heritage of the Jews of Yemen By www.jpost.com Published On :: Thu, 07 Nov 2024 03:00:48 GMT Photographer Zion Ozeri, whose parents immigrated to Israel from Yemen, documents the last generation of a bygone era in The Jews of Yemen – The Last Generation. Full Article photography Yemen yemenite jews Culture in Israel Exhibition
highlight WSL highlights: Man Utd held by Aston Villa By www.bbc.com Published On :: Mon, 11 Nov 2024 00:02:23 GMT Watch highlights as undefeated Manchester United are held to a 0-0 draw by Aston Villa in the WSL. Full Article
highlight New Report Highlights States that Are at the Vanguard of the Reading Revolution By www.the74million.org Published On :: Thu, 13 Jul 2023 09:33:32 EDT A new FutureEd report, The Reading Revolution: How States Are Scaling Literacy Reform, tells the story of how Mississippi, Tennessee and other states at the vanguard of the reading revolution have redesigned reading instruction and raised student achievement in thousands of public schools through bold, state-level leadership. These states have addressed every aspect of early literacy, from how teachers and prospective teachers are trained to the curriculum they use, how students are assessed and whether children are retained rather than promoted to the next grade. Full Article
highlight SaaStr Annual 2019 – Key Takeaways and Highlights By blog.avangate.com Published On :: Tue, 26 Feb 2019 00:00:00 +0000 The 2Checkout team kicked off Feb 2019 by joining over 12,000 participants in San Jose for SaaStr Annual 2019, the largest conference in the world for B2B software. The general consensus of 2Checkout team members at the end of the conference was simple and to the point: “It. Was. Amazing.” Full Article
highlight G20's lack of progress highlights challenge for COP26 By www.chathamhouse.org Published On :: Mon, 01 Nov 2021 11:34:03 +0000 G20's lack of progress highlights challenge for COP26 Expert comment NCapeling 1 November 2021 A positive outcome from the G20 summit was committing to end international financing for coal projects but, on other issues, the communique was ultimately weak. Success at Glasgow depends on bridging fault lines Renata Dwan The G20 summit’s lack of progress on climate highlights the scale of the challenge – and the stakes – for COP26. The countries responsible for 80 per cent of global emissions recognized but failed to agree concrete action to limit global warming to 1.5C. The leaders’ gathering reveals multilateralism’s fault lines. One is the tension between domestic politics and international priorities, reflected in the lack of ambition to reduce coal dependency. The second is the tension between industrialized and developing states over responsibility for delivering global goods. The G20 failed to endorse the G7’s pledge to achieve net zero emissions by 2050 or to accelerate the mobilization of previously agreed climate financing. Success at Glasgow – and beyond – will depend on the extent to which these fault lines can be bridged. Communique’s language was weak Professor Tim Benton A positive outcome on climate from the G20 summit was the commitment to end international financing for coal projects by the end of 2021. This is a win for the climate and for the G20-host, Italy. The G20 might seem disappointing to some, but a lot will depend on expectations The references to 1.5 degrees and the commitment to taking further action this decade were also important, and help lay the groundwork for COP26. On the downside, the communique’s language on phasing out fossil fuel subsidies and coal domestically was very weak. The G20 summit should be regarded as the next step – it is crucial world leaders accelerate their efforts at COP26 in Glasgow to avert climate catastrophe and keep 1.5 degrees alive. Platitudes and vague plans on pandemic preparedness Robert Yates As G20 leaders head to Glasgow to tackle the evolving climate crisis, they leave Rome having failed, yet again, to take serious action to end the ongoing COVID-19 crisis. Despite the obvious urgency to achieve universal vaccine coverage, their communique contains little more than platitudes and vague plans to prepare better for future pandemics. It is not as if they had not been briefed. This year’s G20 leaders’ summit marks a stark contrast with the past four years when much of the group’s energy was exhausted simply trying to maintain a consensus In the run up to the G20, the leaders of the WHO, WTO, IMF, World Bank, former world leaders, Nobel laureates and the Pope, all highlighted the costs of ongoing vaccine inequity: five million deaths next year and $5.3 trillion dollars in lost economic output by 2026. The ask was also straightforward: launch a massive airlift of unneeded vaccines from rich countries through COVAX, ramp up financing of the multilateral response and accelerate technology transfers to developing countries. But on all these issues the wording of the communique is weak, with no numbers on vaccines or funding, no hard timescales and no urgency. This does not augur well for the COP-26 summit. G20 communique is a launching pad Dr Leslie Vinjamuri The G20 might seem disappointing to some, but a lot will depend on expectations. If your starting point is a pandemic that has so far taken five million lives and driven a global economic crisis, and that both these crises are marked by deep inequality, then the measures adopted are bound to look insufficient. But if your starting point is 16 years of democracy in decline, rampant disinformation on issues of climate and public health, four years of failed international leadership during the Donald Trump presidency and, especially today, heightened tensions between the US and China, the world’s two greatest powers, then the fact that the G20 communique achieved as much as it has is remarkable. Any written document that requires agreement between the US, UK, China, Russia, India, Saudi Arabia and the EU is necessarily going to be watered down. But the principles are in the document, and mostly everyone turned up – if some by video. That is a good outcome for this kind of multilateralism in 2021. The G20 communique is a floor not a ceiling, and it’s a launching pad for activism and mobilisation by individual states, but also by corporates, civil society, and subnational actors. Now we need to hope that those on the right side of progress, whether on climate, health, or development, will use this language to drive forward concrete actions towards net zero, climate finance, vaccine distribution, and debt relief. Specifics are for the most part missing Creon Butler This year’s G20 leaders’ summit marks a stark contrast with the past four years when much of the group’s energy was exhausted simply trying to maintain a consensus – in the face of opposition by a President Trump-led United States – on such issues as the importance of tackling climate change, the benefits of the rules-based multilateral trade system and the centrality of the International Monetary Fund (IMF) in the global financial safety net. By contrast, today’s G20 Rome Leaders’ declaration early on underlines ‘the crucial role of multilateralism in finding shared, effective solutions’. In two critical areas for the world economy – the global boost to liquidity from the general allocation of $650bn in Special Drawing Rights and the global tax deal focused on addressing challenges arising from digitalisation – this outlook has been translated into very substantial and concrete achievements announced earlier in the year. Full Article
highlight Citrus Vascular Proteomics Highlights the Role of Peroxidases and Serine Proteases during Huanglongbing Disease Progression By www.mcponline.org Published On :: 2020-12-01 Jessica Y. FrancoDec 1, 2020; 19:1936-1951Research Full Article
highlight Citrus Vascular Proteomics Highlights the Role of Peroxidases and Serine Proteases during Huanglongbing Disease Progression [Research] By www.mcponline.org Published On :: 2020-12-01T00:05:33-08:00 Huanglongbing (HLB) is the most devastating and widespread citrus disease. All commercial citrus varieties are susceptible to the HLB-associated bacterium, Candidatus Liberibacter asiaticus (CLas), which resides in the phloem. The phloem is part of the plant vascular system and is involved in sugar transport. To investigate the plant response to CLas, we enriched for proteins surrounding the phloem in an HLB susceptible sweet orange variety, Washington navel (Citrus sinensis (L) Osbeck). Quantitative proteomics revealed global changes in the citrus proteome after CLas inoculation. Plant metabolism and translation were suppressed, whereas defense-related proteins such as peroxidases, proteases and protease inhibitors were induced in the vasculature. Transcript accumulation and enzymatic activity of plant peroxidases in CLas infected sweet orange varieties under greenhouse and field conditions were assessed. Although peroxidase transcript accumulation was induced in CLas infected sweet orange varieties, peroxidase enzymatic activity varied. Specific serine proteases were up-regulated in Washington navel in the presence of CLas based on quantitative proteomics. Subsequent activity-based protein profiling revealed increased activity of two serine proteases, and reduced activity of one protease in two C. sinensis sweet orange varieties under greenhouse and field conditions. The observations in the current study highlight global reprogramming of the citrus vascular proteome and differential regulation of enzyme classes in response to CLas infection. These results open an avenue for further investigation of diverse responses to HLB across different environmental conditions and citrus genotypes. Full Article