Numsa and Sacca have welcomed the Labour Court's ruling that the retrenchment process initiated by the SAA business rescue practitioners was unfair.

Georgia summoned its ambassador to Ukraine back to Tbilisi in a sign of its displeasure over Kyiv’s decision to name Mikheil Saakashvili to a government reform body. 

Masaajidyada ku yaal Jarmalka ayaa dib loogu furi doona albaabada si ay ugu cibaadeysantaan dadka Muslimiinta ah, arrintan ayaa imaanaysa ka dib go’aan ka soo baxay dawladda oo lagu fududeynayo xannibaadda Jarmalka lagu soo rogey kadib Fayraska Korona. Masaajida ku yaal dalka ayaa si tartiib tartiib ah dib loogu furi doona iyadoo la raacayo tilmaamaha […]

The post Muslimiinta Jarmalka oo isu diyaarinaya dib u furitaanka Masaajidda appeared first on Horseed Media • Somali News.

Soomaaliya waxa ay guddoontay maalintii Khamiis ahayd (7 May) shixnaddii labaad oo ah kaalmo agab caafimaad oo ay ku deeqday Dowladda Jamhuuriyadda Shacbiga Shiinaha si looga hortago COVID-19, iyadooo ka koobnayd maaskaro, galoofyo, dareeraha gacmaha lagu nadiifiyo, qalabka baaridda-caafimaad iyo dharka ilaalinta leh ee loogu talogalay in ay ku labistaan xirfadlayaasha caafimaadka. Tan iyo 24 […]

The post Shiinaha oo Soomaaliya soo gaarsiiyey deeq caafimaad appeared first on Horseed Media • Somali News.

Dalka Talyaaniga ayaa noqday wadankii ugu horeeyay ee ka tirsan Ururka Midowga Yurub oo diiwaangeliyay in cudurka Coronavirus ay ugu dhinteen dad gaaraya soddon kun oo ruux sida la xaqiijiyay, Maanta oo keli ah 243 ruux ayaa dhimashadooda, la diiwaangeliya geeriyootay dalka Talyaaniga. Maalin walba tirada cudurkan laga heli jirey dalka Talyaaniga ayaa hoos ugu […]

The post Tirada dadka ugu dhintey Covid-19 Talyaaniga oo kor u dhaaftay soddon kun oo ruux. appeared first on Horseed Media • Somali News.

Wasiirka Shaqada iyo Arrimaha bulshada ee Xukumadda Federaalka Soomaaliya Sadiiq Xirsi warfaa ayaa sheegay in si habsami leh uu u socdo barnaamijka Baxnaano oo loogu talagalay in lagu kaalmeeyo dadka danyara ah. Wasiirka Warfaa ayaa sheegay in mashruucaan uu yahay mid ay leedahay Dowladda Soomaaliyeed, isla markaana uu taageero ka heysto Bankiga aduunka waxaana bishii […]

The post Dowladda Soomaaliya oo sheegtay in si habsami leh uu u socdo barnaamijka baxnaano. appeared first on Horseed Media • Somali News.

Taliyiha ciidanka Boolis magaalada Dhuusamareeb ee maamul goboleedka Galmudug Burhaan Axmed ayaa iska casaley xilkii uu degmada ka hayey kadib markii rag ka tirsan shabakad isku xiran oo al-Shabaab ah oo uu soo qabtey xabsiga laga sii daayey iyada oo la diidey in wax baaritaan ah la mariyo. ”Waxaa xabsiga Dhuusamareeb laga sii daayay laba […]

The post Taliye Boolis oo isu casiley rag al-Shabaab ah oo xabsiga laga siidaayey -VIDEO appeared first on Horseed Media • Somali News.

Diyaaraddii ku buburtey Berdaale ee nooceedu ahaa xamuulka ayaa la xaqiijiyey in ciidamada Itoobiya ee halkaas ku sugan ay ku rideen hubka lidka diyaaradaha kadibna sidaas ku burburtey. War ka soo baxay AMISOM ee la xiriira baaritaanka wada jirka ah ee la sameynaayey ayaa lagu sheegay in diyaaradda aysan soo ridin ciidamada AMISOM ee ay […]

The post Diyaaraddii Berdaale ku dhacdey oo la xaqiijiyey in Ciidamada Itoobiya soo rideen appeared first on Horseed Media • Somali News.

Laba wasiir oo ka tirsan xukuumadda Puntland ayaa todobaadkan laga heley fayraska korona ee caalamka faro ba’an ku haya. Labada wasiir ayaa kamid ahaa dadkii sida weyn ula shaqeynaayey bulshada aadna ugu dhawaa gaar ahaan dhibaatadii ka dhacdey magaalada Qardho. Madaxweynaha Puntland Siciid Cabdullaahi Deni ayaa sheegay in wasiiradan xaaladooda caafimaad aad u wanaagsan tahay, […]

The post Waa sidee xaaladda Wasiirada Puntland ee laga helay COVID-19? appeared first on Horseed Media • Somali News.

Ciidamada PSF ayaa Bosaso ku qabtey gaari lagu soo raray waxyaabaha qaraxa dhaliya, iyo bobooyinka gacanta laga tuuro. Wararku waxay sheegayaan in ciidanka PSF ay muddo ku dabo jireen shabakad argagixiso ah oo doonaayey in ay qaraxyo ka fuliyaan gudaha Puntland. War ka soo baxay ciidanka amniga Puntland ee PSF ayaa sheegay Intii hawlgalku socday, […]

The post Ciidamada PSF oo fashiliyey weerar argagixiso gudaha Boosaaso appeared first on Horseed Media • Somali News.

Madaxweynaha Puntland Siciid Cabdullaahi Deni ayaa maanta shir saxaafadeed uu ku qabtey magaalada Qardho uga hadley xiisada colaadeed ee weli ka taagan deegaan Teegare oo galbeed uga beegan magaalada Gaalacyo xarunta gobolka Mudug. Madaxweyne Deni ayaa sheegay in ay ballan qaadayaan in ay xakameeyaan dhankooda, sugayaan amniga, waxaana uu dadka deegaankaas ee reer Puntland ugu […]

The post Madaxweynaha Puntland oo ka hadley xiisadda colaadeed ee gobolka Mudug appeared first on Horseed Media • Somali News.

1. Keh Gayi Sorry teaser: Shehnaaz Gill pairs up with Jassie Gill in new music video, watch  Hindustan Times
2. Keh Gayi Sorry Teaser: Fans can't wait for the full music video featuring Shehnaaz Gill and Jassie Gill  PINKVILLA
3. Bigg Boss 13's Shehnaaz Gill curses Coronavirus for ruining her dreams in this new hilarious TikTok video  Bollywood Life
4. Shehnaz Gill curses corona for ruining her dream of signing autographs after Bigg Boss 13; watch hilariou  Times of India
5. PHOTOS: Shehnaaz Gill proves she can pull off any look with panache; Check out  PINKVILLA
6. View Full coverage on Google News

Turkey's historic Grand Bazaar will reopen for shopping as of June 1, the chairman of the iconic tourist destination said on May 8. 

Hong Kong’s Canto-pop “dancing king” Aaron Kwok Fu-shing’s online charity concert on Saturday afternoon to help the city’s struggling dancers attracted about 2 million viewers and raised more than HK$1 million, amid local signs of an easing coronavirus threat.Kwok’s onstage moves are known to be inspired by Michael Jackson. The one-hour performance by the singer-dancer-actor, 54, flanked by about 100 dancers, was live-streamed on multiple channels on social media from 5pm to 6pm.He performed on…

Tamannaah Bhatia has quashed the chitchat about her love life

Kartik Aaryan considers 'Love Aaj Kal' opposite Sara Ali Khan as the best performance in his career so far

The video has won the hearts on social media and garnered thousands of hearts within no time.

To reduce air pollution, national and city government policy makers, their development partners, academe, and the private sector need to work with young people. 

LX7167A

Former Prime Minister Saad Hariri Thursday dismissed as not useful the meeting held the previous day at Baabda Palace to discuss the economic financial rescue plan which he and his parliamentary Future bloc boycotted.

mXT2912TDAT_0x38_2.0.AA_PROD.enc.zip

mXT2113TDAT_0x3E_2.0.AA_PROD.enc.zip

For more than 70 years, some of world’s most respected musicians, producers and DJs have used HARMAN’s exceptional audio solutions and products from the studio to the stage. In honor of Make Music Day 2019, we sat down with Aasheesh Paliwal of HARMAN...

BANGALORE, INDIA –  HARMAN (NYSE:HAR), the premier connected technologies company for automotive, consumer and enterprise markets, today announced the launch of limited edition JBL® Raaga Synchros S500AR headphones, autographed by academy award winner...

Van knallende baslijnen tot vloeiende gitaarsolo’s, JBL introduceert de JBL Link Portable en Link Music. Ze zijn net zo handig als ze mooi zijn, de speakers zijn voorzien van 360 graden JBL’s kenmerkende sound, Wifi- en Bluetooth-connectiviteit en ingebouwde Chromecast en Google assistent.

360° JBL geluid. Een fantastische lichtshow. Met de Pulse 4 van JBL zorg jij voor een goede sfeer, of je nu wilt feesten of chillen. Van woonkamer tot strandfeest (en alles daartussenin), de nieuwe JBL Pulse 4 staat voor je klaar.

The three-body problem has vexed mathematicians and physicists for 300 years, but AI can find solutions far faster than any other method anyone has come up with

College sports will not resume until all students are back on campus, NCAA president Mark Emmert said Friday.

HARMAN is gearing up to present its best-in-class audio technologies and solutions for many of the world’s foremost automotive OEMs at IAA 2019 from September 12-22 in Frankfurt/Main. Now in its 68th edition, IAA is the world’s largest motor show as well...

Analysts are forecasting that 250 million connected cars will be on the road as early as 2020. At the same time, the magnitude of data transfers between these cars and their environment will only continue to grow. The automotive industry is at a tipping...

College sports will not resume until all students are back on campus, NCAA president Mark Emmert said Friday.

The United States' Federal Aviation Administration (FAA) is investigating an incident in which actor Harrison Ford was piloting a small plane that wrongly crossed a runway where another aircraft was landing, U.S. media reported late on Wednesday citing an FAA statement.

Title: Skyrizi (risankizumab-rzaa)
Category: Medications
Created: 4/20/2020 12:00:00 AM
Last Editorial Review: 4/20/2020 12:00:00 AM

ABSTRACT

The appressoria that are generated by the rice blast fungus Magnaporthe oryzae in response to surface cues are important for successful colonization. Previous work showed that regulators of G-protein signaling (RGS) and RGS-like proteins play critical roles in appressorium formation. However, the mechanisms by which these proteins orchestrate surface recognition for appressorium induction remain unclear. Here, we performed comparative transcriptomic studies of Morgs mutant and wild-type strains and found that M. oryzae Aa91 (MoAa91), a homolog of the auxiliary activity family 9 protein (Aa9), was required for surface recognition of M. oryzae. We found that MoAA91 was regulated by the MoMsn2 transcription factor and that its disruption resulted in defects in both appressorium formation on the artificial inductive surface and full virulence of the pathogen. We further showed that MoAa91 was secreted into the apoplast space and was capable of competing with the immune receptor chitin elicitor-binding protein precursor (CEBiP) for chitin binding, thereby suppressing chitin-induced plant immune responses. In summary, we have found that MoAa91 is a novel signaling molecule regulated by RGS and RGS-like proteins and that MoAa91 not only governs appressorium development and virulence but also functions as an effector to suppress host immunity.

IMPORTANCE The rice blast fungus Magnaporthe oryzae generates infection structure appressoria in response to surface cues largely due to functions of signaling molecules, including G-proteins, regulators of G-protein signaling (RGS), mitogen-activated protein (MAP) kinase pathways, cAMP signaling, and TOR signaling pathways. M. oryzae encodes eight RGS and RGS-like proteins (MoRgs1 to MoRgs8), and MoRgs1, MoRgs3, MoRgs4, and MoRgs7 were found to be particularly important in appressorium development. To explore the mechanisms by which these proteins regulate appressorium development, we have performed a comparative in planta transcriptomic study and identified an auxiliary activity family 9 protein (Aa9) homolog that we named MoAa91. We showed that MoAa91 was secreted from appressoria and that the recombinant MoAa91 could compete with a chitin elicitor-binding protein precursor (CEBiP) for chitin binding, thereby suppressing chitin-induced plant immunity. By identifying MoAa91 as a novel signaling molecule functioning in appressorium development and an effector in suppressing host immunity, our studies revealed a novel mechanism by which RGS and RGS-like proteins regulate pathogen-host interactions.

Escherichia coli ribosomal protein (r-protein) L4 has extraribosomal biological functions. Previously, we described L4 as inhibiting RNase E activity through protein-protein interactions. Here, we report that from stabilized transcripts regulated by L4-RNase E, mRNA levels of tnaA (encoding tryptophanase from the tnaCAB operon) increased upon ectopic L4 expression, whereas TnaA protein levels decreased. However, at nonpermissive temperatures (to inactivate RNase E), tnaA mRNA and protein levels both increased in an rne temperature-sensitive [rne(Ts)] mutant strain. Thus, L4 protein fine-tunes TnaA protein levels independently of its inhibition of RNase E. We demonstrate that ectopically expressed L4 binds with transcribed spacer RNA between tnaC and tnaA and downregulates TnaA translation. We found that deletion of the 5' or 3' half of the spacer compared to the wild type resulted in a similar reduction in TnaA translation in the presence of L4. In vitro binding of L4 to the tnaC-tnaA transcribed spacer RNA results in changes to its secondary structure. We reveal that during early stationary-phase bacterial growth, steady-state levels of tnaA mRNA increased but TnaA protein levels decreased. We further confirm that endogenous L4 binds to tnaC-tnaA transcribed spacer RNA in cells at early stationary phase. Our results reveal the novel function of L4 in fine-tuning TnaA protein levels during cell growth and demonstrate that r-protein L4 acts as a translation regulator outside the ribosome and its own operon.

IMPORTANCE Some ribosomal proteins have extraribosomal functions in addition to ribosome translation function. The extraribosomal functions of several r-proteins control operon expression by binding to own-operon transcripts. Previously, we discovered a posttranscriptional, RNase E-dependent regulatory role for r-protein L4 in the stabilization of stress-responsive transcripts. Here, we found an additional extraribosomal function for L4 in regulating the tna operon by L4-intergenic spacer mRNA interactions. L4 binds to the transcribed spacer RNA between tnaC and tnaA and alters the structural conformation of the spacer RNA, thereby reducing the translation of TnaA. Our study establishes a previously unknown L4-mediated mechanism for regulating gene expression, suggesting that bacterial cells have multiple strategies for controlling levels of tryptophanase in response to varied cell growth conditions.

A new larger benthic porcelaneous foraminifer of soritid affinity is described as Tarburina zagrosiana n. gen., n. sp. from the late Maastrichtian of the Tarbur Formation, Zagros Zone, SW Iran. It occurs in foraminiferal–dasycladalean wackestones and packstones, in association with Loftusia ssp., dicyclinids/cuneolinids, Neobalkhania bignoti Cherchi & Schroeder, Gyroconulina columellifera Schroeder & Darmoian, Spirolina? farsiana Schlagintweit & Rashidi, Broeckina cf. dufrenoyi (d'Archiac), other benthic foraminifers, and dasycladalean algae. Due to its elongate test and marginal chamber subdivision by aligned vertical partitions, Tarburina n. gen. can be compared with representatives of the Praerhapydionininae. The interio-marginal slit-like foramina/aperture of Tarburina represents an outstanding feature in complex porcelaneous taxa. The monospecific genus Tarburina is considered a Maastrichtian newcomer within the Late Cretaceous Global Community Maturation cycle of larger benthic foraminifera. A biostratigraphic and palaeobiogeographical restriction seems possible, as reported for many other Late Cretaceous larger benthic foraminifera.

OBJECTIVE

Low heart rate variability (HRV), a marker for cardiac autonomic dysfunction, is a known feature of type 2 diabetes, but it remains incompletely understood whether this also applies to prediabetes or across the whole glycemic spectrum. Therefore, we investigated the association among prediabetes, type 2 diabetes, and measures of glycemia and HRV.

Nayeli G. Reyes-Nava, Hung-Chun Yu, Curtis R. Coughlin II, Tamim H. Shaikh, and Anita M. Quintana

We used whole-exome sequencing (WES) to determine the genetic etiology of a patient with a multi-system disorder characterized by a seizure phenotype. WES identified a heterozygous de novo missense mutation in the GABRA1 gene (c.875C>T). GABRA1 encodes the alpha subunit of the gamma-aminobutyric acid receptor A (GABA_AR). The GABA_AR is a ligand gated ion channel that mediates the fast inhibitory signals of the nervous system, and mutations in the subunits that compose the GABA_AR have been previously associated with human disease. To understand the mechanisms by which GABRA1 regulates brain development, we developed a zebrafish model of gabra1 deficiency. gabra1 expression is restricted to the nervous system and behavioral analysis of morpholino injected larvae suggests that the knockdown of gabra1 results in hypoactivity and defects in the expression of other subunits of the GABA_AR. Expression of the human GABRA1 protein in morphants partially restored the hypomotility phenotype. In contrast, the expression of the c.875C>T variant did not restore these behavioral deficits. Collectively, these results represent a functional approach to understand the mechanisms by which loss-of-function alleles cause disease.

Background and objectives

Although renin-angiotensin-aldosterone system inhibition (RAASi) is a cornerstone in the treatment of children with CKD, it is sometimes discontinued when kidney function declines. We studied the reasons of RAASi discontinuation and associations between RAASi discontinuation and important risk markers of CKD progression and on eGFR decline in the Cardiovascular Comorbidity in Children with CKD study.

Adeno-associated virus (AAV) recombinants are currently the vector of choice for many gene therapy applications. As experimental therapies progress to clinical trials, the need to characterize recombinant adeno-associated viruses (rAAVs) accurately and reproducibly increases. Accurate determination of rAAV infectious titer is important for determining the activity of each vector lot and for ensuring lot-to-lot consistency. The following protocol developed in our laboratory uses a 96-well TCID₅₀ format and quantitative polymerase chain reaction (qPCR) detection for the determination of rAAV infectious titer.

This protocol is used to determine the concentration of DNase-resistant vector genomes (i.e., packaged in the capsid) in purified recombinant adeno-associated virus (rAAV) preparations. The protocol begins with treatment of the vector stock with DNase I to eliminate unencapsidated AAV DNA or contaminating plasmid DNA. This is followed by a heat treatment to heat-inactivate DNase I, to disrupt the viral capsid, and to release the packaged vector genomes for quantification by real-time polymerase chain reaction (PCR) using a set of standards (linearized plasmid used for vector production) containing known copy numbers. To accomplish high-throughput titration, the primer and probe sets used in real-time PCR are usually designed to target common elements present in most rAAV genomes, such as promoters and poly(A) signals. This strategy significantly reduces the number of PCRs, controls, and turnaround time. Several important controls should be included in the assay as follows: The first two controls should have a known copy number of the rAAV genome plasmid treated or not treated with DNase I. This control tests the effectiveness of DNase treatment. To control for potential cross-contamination between samples during the preparation process, a blank control containing nuclease-free water only should be processed and tested in parallel. A validation vector sample with a known titer should be included in every assay to monitor interassay variability. Finally, for the PCR run, a no-template control (NTC) is included to indicate cross-contamination during PCR setup.

This protocol describes a simple single-step column purification (SSCP) of rAAV2 by gravity flow based on its affinity to heparin, without ultracentrifugation.

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Grand Theft Auto V has once again taken the top spot on the EMEAA charts for week 17, 2020. XCOM: Chimera Squad is in second place, Call of Duty: Modern Warfare is in third, Alien: Isolation is in fourth, and Animal Crossing: New Horizons rounds out the top five.

Here are the top 5 best-selling titles (combined physical and digital sales) in EMEAA:

1. Grand Theft Auto V
2. XCOM: Chimera Squad
3. Call of Duty: Modern Warfare
4. Alien: Isolation
5. Animal Crossing: New Horizons

A life-long and avid gamer, William D'Angelo was first introduced to VGChartz in 2007. After years of supporting the site, he was brought on in 2010 as a junior analyst, working his way up to lead analyst in 2012. He has expanded his involvement in the gaming community by producing content on his own YouTube channel and Twitch channel dedicated to gaming Let's Plays and tutorials. You can contact the author at wdangelo@vgchartz.com or on Twitter @TrunksWD.

Full Article - https://www.vgchartz.com/article/443399/grand-theft-auto-v-once-again-tops-the-emeaa-charts/