The quinoprotein glycine oxidase from the marine bacterium Pseudoalteromonas luteoviolacea (PlGoxA) uses a protein-derived cysteine tryptophylquinone (CTQ) cofactor to catalyze conversion of glycine to glyoxylate and ammonia. This homotetrameric enzyme exhibits strong cooperativity toward glycine binding. It is a good model for studying enzyme kinetics and cooperativity, specifically for being able to separate those aspects of protein function through directed mutagenesis. Variant proteins were generated with mutations in four active-site residues, Phe-316, His-583, Tyr-766, and His-767. Structures for glycine-soaked crystals were obtained for each. Different mutations had differential effects on kcat and K0.5 for catalysis, K0.5 for substrate binding, and the Hill coefficients describing the steady-state kinetics or substrate binding. Phe-316 and Tyr-766 variants retained catalytic activity, albeit with altered kinetics and cooperativity. Substitutions of His-583 revealed that it is essential for glycine binding, and the structure of H583C PlGoxA had no active-site glycine present in glycine-soaked crystals. The structure of H767A PlGoxA revealed a previously undetected reaction intermediate, a carbinolamine product-reduced CTQ adduct, and exhibited only negligible activity. The results of these experiments, as well as those with the native enzyme and previous variants, enabled construction of a detailed mechanism for the reductive half-reaction of glycine oxidation. This proposed mechanism includes three discrete reaction intermediates that are covalently bound to CTQ during the reaction, two of which have now been structurally characterized by X-ray crystallography.

Background:

The success of multisite collaborative research relies on effective data collection, harmonization, and aggregation strategies. Data Coordination Centers (DCC) serve to facilitate the implementation of these strategies. The utility of a DCC can be particularly relevant for research on rare diseases where collaboration from multiple sites to amass large aggregate datasets is essential. However, approaches to building a DCC have been scarcely documented.

Yell at yer friends in 1-4 player co-op while working under time pressure to serve up meals to an unforgiving god in Overc—er sorry, Cannibal Cuisine. It’s certainly got a familiar vibe to it, so if you liked the frantic food prep of that other friendship-ender perhaps you’ll like doing it with a new secret […]

Soft finger-like gripper robots have been engineered to sweat when hot and are able to cool down almost three times more efficiently than humans

Electric scooters are a nightmare. Rented by the minute, they clog up pavements and are an ungainly eyesore, but we still need them, says Donna Lu

Black paint usually absorbs heat, but a new two-layer polymer paint reflects infrared light and keeps objects 16°C cooler, which could help make buildings more energy efficient

Skimming system tripled previous best effort at oil collection.

Approval draws criticism over transparency and safety tests.

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Some labels are showing how fashion can put the planet first. To celebrate Earth Day, here are a few of our favourites

An analysis of the fossilized teeth of Deinonychus antirrhopus, a species of wolf-sized dromaeosaurid dinosaur that lived between 115 and 108 million years ago (Cretaceous period) in what is now the United States, adds to the growing evidence that this and other raptors were not complex social hunters by modern mammalian standards. The image of [...]

The singer will present the 10-episode show from her kitchen.

There are all sorts of ways the home cook can help conserve precious energy, this recipe for vegan coconutty ‘cheesecake’ being a case in point

Reducing waste in the kitchen isn’t just about saving food: the time and energy it takes to cook food are also important resources that are easily squandered.

The best way to save energy, besides loving your leftovers, is by cooking less: swap out a cooked element of your meal for a nutritious vegetable salad or raw dish (such as today’s coconut and lemon “cheesecake”); or make one-pot wonders that don’t use multiple cooker rings. And when you do need to cook more, make extra portions for the freezer.

The Advertising Standards Agency (ASA) found the fast food giant had also failed to make it immediately clear its new product was dressed with an egg-based mayonnaise.

MPs will investigate the potential consequences of allowing electric scooters to be used on the road.

In today's Diary: how Whitehall's cats are coping with coronavirus / Russell Crowe sideswipes Oliver Reed / Angela Scanlon on the glory of cheese / Michael Fabricant vows to wear pink shorts

One of Norway's wealthiest men is being held on suspicion of murdering his wife who has been missing since 2018.

In today's Diary: pub quizzers celebrate online surge / Mel Giedroyc's nightmare day / Boris Johnson on how many children he has / Peers show off their Zoom backgrounds

The Duchess of Cambridge has made a virtual bedside visit to new parents during a video call to midwives she worked alongside last year.

You can donate at virginmoneygiving.com/fund/FoodforLondonNOW

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In stock right now (8am CST 4/15/2020) $39.99 plus $5 shipping.

There’s been a bunch of scuttlebutt around this game being delisted from the eShop, supposedly mining bitcoin and a disagreement between the publisher and the IP owner. No matter the truth, grab it at retail pricing and don’t pay scalpers on eBay.

Crash Bandicoot N. Sane Trilogy (Xbox One) for $19.99

Uber lost US$2.9 billion in the first quarter as its overseas investments were hammered by the coronavirus pandemic, but the company is looking to its growing food delivery business as well as aggressive cost-cutting to ease the pain.

Move over, Martha Stewart and Gwyneth Paltrow. There's a new feud brewing between two leaders in the lifestyle industry. Best-selling cookbook author Alison Roman has caught the...

Follow our live coronavirus updates HERE Coronavirus: The symptoms

As a longtime Paris resident, at first I feared Dirt might be yet another expat tale of moving to France en famille, with all its tedious clichés. I should have known better.

Chef inadvertently started a blaze in his kitchen as Holly Willoughby and Phillip Schofield looked on in shock

Parsons stars in Ryan Murphy's new Netflix series, Hollywood

Lockdown Britain has embraced the sausage, with sales up 33%. But there’s much more you can do with them than fry-ups, sandwiches and casseroles

When you Google famous quotes about sausages (say you need an opener for an article), one of the first comes from Phoebe Waller-Bridge, who apparently once said: “Sausages are just funny. I don’t know why. I can’t explain it.”

Waller-Bridge is not wrong. Sausages are inherently funny. But their comedic value is also what holds them back. In the kitchen, no one takes sausages seriously. You very rarely see anyone serving sausages on Come Dine With Me; it would be an act of self-sabotage. You cannot win with sausages. They’re a culinary joke, unrefined, a bit naff.

To mark 250 years since British explorers landed in Australia for the first time, authorities are planning to unveil new memorials at Captain James Cook's landing site in Botany Bay, while a replica Endeavour sailing vessel will circumnavigate Australia – when Covid-19 restrictions allow. But one Aboriginal elder, who grew up on the shores of Botany Bay and has spent years involved in the resurrection of his Indigenous Dharawal culture, explains why Aboriginal people will not be celebrating

• Paul Daley: Commemorating James Cook’s arrival, Australia should not omit his role in the suffering that followed

In his speech, Tim Cook offered advice for navigating challenging circumstances in the years ahead and talked about his career at Apple.

The world of online cookery has stepped up its game

Brighten up your new WFH space with some fragrant florals

The Michelin-starred chef is making a three-course spring feast

Buying second hand is not only one of the most sustainable ways to shop for clothes, but it's increasingly becoming the best way to find unique and original pieces.

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Who needs pretentious chefs? After a decade away, TV’s simplest cookery show is on the air again, and with Rylan at the helm it’s the perfect recipe for success

This week, the first new episodes of Ready Steady Cook for a decade are broadcast on BBC One. The miraculous thing is that, watching it, you’d never know that it ever went away.

Sure, some things are little different. The budget for the ingredients has risen from £5 to a colossal £7.50, and they are presented in reusable totes rather than single-use plastic bags. The theme tune now comes with a weird techno burble that makes you feel as if you are playing an imported PlayStation 2 game about different methods of cooking mince. Sumac exists. And there is a new host in Rylan Clark-Neal, continuing his monomaniacal quest to seize and hijack every defunct daytime gameshow made during the 1990s.

Manchester United staff are assisting the club's players with everything from psychological support to cooking lessons during the coronavirus lockdown.

Move over, Martha Stewart and Gwyneth Paltrow. There's a new feud brewing between two leaders in the lifestyle industry. Best-selling cookbook author Alison Roman has caught the...

Multi-function retro calculator watches.

You need special training and licensing to fly a drone this intense.

At 14 years of age Zaynn Bird was a sub-par McDonald's employee. At 23 he owns one of his state's most impressive burger joints.