IBM (NYSE: IBM) today announced a business consulting and technology partnership with Yarra Valley Water to enhance the management and functionality of its assets and customer service to over 1.7 million people and 50,000 businesses throughout Melbourne now and in the future. Through this partnership Yarra Valley Water will gain access to critical insights to maximise asset performance and improve customer service outcomes across its operations.

Just a short note that the memorial article “Analysis and applications: The mathematical work of Elias Stein” has just been published in the Bulletin of the American Mathematical Society.  This article was a collective effort led by Charlie Fefferman, Alex Ionescu, Steve Wainger and myself to describe the various mathematical contributions of Elias Stein, who […]

Next quarter, starting March 30, I will be teaching “Math 247B: Classical Fourier Analysis” here at UCLA.  (The course should more accurately be named “Modern real-variable harmonic analysis”, but we have not gotten around to implementing such a name change.) This class (a continuation of Math 247A from previous quarter, taught by my colleague, Monica […]

Analysis: Tennis pros' US return amid pandemic no true model

Oh man, this is great! It's perfect for... seeing texts the second they come in? Showing off your cell phone? Watching videos in a horrid orientation? Wait a minute, why is this useful again?

~NSHA

Einstein’s derivation of the Lorentz Transformation is purely theoretical. This study shows how it is related to the physical phenomenon of time dilation and length contraction. The Lorentz Transformation was first derived using the conditions of time dilation and length contraction. Later, Albert Einstein has given a different derivation of the Lorentz Transformation by using...

Systematic review produced by the EPPI-Centre in 2015.This systematic review aimed to evaluate the effect of HAART and ARV monotherapy on liver disease progression and liver-related mortality in individuals co-infected with HIV and hepatitis C, including in patients with haemophilia.

Recent experiences with combustible gas releases in residential buildings have led to a proposal for NFPA Standards Development for locating combustible gas detectors and consensus on installation location requirements. NFPA is considering a proposed

In a few weeks, I will need to stop dithering and make a decision: will I use an analog or digital planner for 2019? When I started my current job at the library, I had a Bullet Journal system in place, which satisfied my two primary drives: recording notes and ideas by hand, because I... Continue Reading →

Advocates have long protested the lack of special education pre-K classes for 3- and 4-year-olds, which is federally mandated, even as the city invests millions in universal pre-K.

A new study reveals that some European Neanderthals might have displaced their relatives in Siberia, while others mingled with another, still mysterious, ancient human population.

Researchers are using fluid dynamics to try to improve the study of crime scene blood spatter.

The Clippers spent two years building a contender around Kawhi Leonard and Paul George. Can they still compete for a title if the NBA season resumes?

LeBron James, Anthony Davis and the Lakers made a steady run to the top of the Western Conference. If the NBA season resumes, can they contend for a title?

Morgan Stanley stock analyst Adam Jonas told clients Wednesday that if Tesla's troubles continue, it's unlikely to be acquired by a tech company, an auto company, or someone from China.

In the "pedo guy" trial, Musk's lawyers dismissed Twitter as a "not a source of facts."

MERVYN KING, the former governor of the Bank of England, once issued a brutal analysis of the global banking system and argued for its reinvention, it can be revealed as the Government fine-tunes its economic response to the coronavirus pandemic.

DFT (Design for testing) Engineer: 4-6 years of experience Experience with Synopsys Toolset is mandatory. Scan insertion, Debug DRC, ATPG patterns Gate Level Simulation ( timing and no-timing) Knowledge in P1500 and JTAG is an advantage. Knowledge in MBIST is an advantage. Phy

HCL Global Systems Inc. has job opening through headquarters in Farmington Hills, MI for a full-time Sr. Test Analyst to design, develop, test and implement client-servers and web-based software application systems. Provide support for databases for HPP reporting application, development, QA and pro

DUTIES: **All applications must be submitted by January 31, 2018, at http://www.mdc.mo.gov/about/jobs. **Any applications submitted through external sources will not be reviewed. Works as a member of the Infrastructure Asset Management Program Team and supports the Design and Development Divisi

Accelerate, the 5-2 favorite in the final Breeders' Cup Classic future wagering pool, is coming off a big win as the 3-10 favorite in the Awesome Again Stakes at Saratoga. But should you bet him?

What are the main differences between a European canal hotel barge cruise and a river cruise? I think there are 8 key things you need to know that makes a canal cruise different to a river cruise. By understanding how they differ you can better decide is a river cruise or canal hotel barge cruise is best for your next European river cruising vacation

Note: I travelled as a guest of CroisiEurope on a 6-night hotel barge cruise in France before making this video Croisieurope had no input into the opinions or content of this video.

** Buy my Cruise T-shirts: http://bit.ly/TFTStore
** USA cruisers get great cruise deals CRUISEDIRECT.COM: http://bit.ly/TFTBookCruise
** UK Cruisers get great cruise deals with CRUISE.CO.UK: http://bit.ly/BookCruiseUK

Gary Bembridge's Tips For Travellers aims to help you make more of your precious travel time and money on land and when cruising the oceans or rivers of the world. To help you, in every video I draw on my first-hand tips and advice from travelling every month for over 20 years and 60+ cruises.

Follow Tips For Travellers on:
- Instagram: http://www.instagram.com/garybembridge
- Facebook: http://www.facebook.com/tipsfortravellers
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strataconf: Building Big Data applications now easier http://t.co/jaATalPL4P Hadoop analytic engines simplify creation of low-cost, scalable apps

velocityconf: New #velocityconf CA program preview is up: http://t.co/rKjf91RXdD @ariyahidayat on End-to-End JS Quality Analysis.

The Amateur Traveler  talks to Richard Graw about his experiences cruising the canals of England in a canal boat (or narrow boat). The canals traverse through tunnels, up staircases of locks and even up over aqueducts.

Hear about travel to The Pantanal in Brazil as the Amateur Traveler talks to Daisy about her recent trip to this vast wetlands area. The Pantanal is south of the Amazon in Eastern Brazil and also extends into Bolivia and Paraguay.

“The Pantanal is a basin that is the world’s largest wetlands. It is about ten times the size of the Everglades. It’s really a great site to see wildlife.” The Pantanal is a UNESCO World Heritage Site.

Hear about travel to The Canal du Midi and Carcassonne in southern France as Chris from the Amateur Traveler talks about his recent luxury hotel barge cruise.

Invitation Only Research Event

Event participants

Dr Lina Khatib, Head, Middle East and North Africa Programme, Chatham House
Dr Renad Mansour, Research Fellow, Middle East and North Africa Programme, Chatham House

12 March 2015 , Volume 91, Number 2

Joe Carroll
Feb 1, 2003; 2:117-126
Research

Boris Macek
Feb 1, 2008; 7:299-307
Research

Tamar Geiger
Mar 1, 2012; 11:M111.014050-M111.014050
Special Issue: Prospects in Space and Time

Lawrence E. Ostrowski
Jun 1, 2002; 1:451-465
Research

Qiang Tian
Oct 1, 2004; 3:960-969
Research

Ludovic C. Gillet
Jun 1, 2012; 11:O111.016717-O111.016717
Research

Claudio P. Albuquerque
Jul 1, 2008; 7:1389-1396
Research

Linn Fagerberg
Feb 1, 2014; 13:397-406
Research

In cyanobacteria, metabolic pathways that use the nitrogen-rich amino acid arginine play a pivotal role in nitrogen storage and mobilization. The N-terminal domains of two recently identified bacterial enzymes: ArgZ from Synechocystis and AgrE from Anabaena, have been found to contain an arginine dihydrolase. This enzyme provides catabolic activity that converts arginine to ornithine, resulting in concomitant release of CO2 and ammonia. In Synechocystis, the ArgZ-mediated ornithine–ammonia cycle plays a central role in nitrogen storage and remobilization. The C-terminal domain of AgrE contains an ornithine cyclodeaminase responsible for the formation of proline from ornithine and ammonia production, indicating that AgrE is a bifunctional enzyme catalyzing two sequential reactions in arginine catabolism. Here, the crystal structures of AgrE in three different ligation states revealed that it has a tetrameric conformation, possesses a binding site for the arginine dihydrolase substrate l-arginine and product l-ornithine, and contains a binding site for the coenzyme NAD(H) required for ornithine cyclodeaminase activity. Structure–function analyses indicated that the structure and catalytic mechanism of arginine dihydrolase in AgrE are highly homologous with those of a known bacterial arginine hydrolase. We found that in addition to other active-site residues, Asn-71 is essential for AgrE's dihydrolase activity. Further analysis suggested the presence of a passage for substrate channeling between the two distinct AgrE active sites, which are situated ∼45 Å apart. These results provide structural and functional insights into the bifunctional arginine dihydrolase–ornithine cyclodeaminase enzyme AgrE required for arginine catabolism in Anabaena.

Numerous zinc ectoenzymes are metalated by zinc and activated in the compartments of the early secretory pathway before reaching their destination. Zn transporter (ZNT) proteins located in these compartments are essential for ectoenzyme activation. We have previously reported that ZNT proteins, specifically ZNT5–ZNT6 heterodimers and ZNT7 homodimers, play critical roles in the activation of zinc ectoenzymes, such as alkaline phosphatases (ALPs), by mobilizing cytosolic zinc into these compartments. However, this process remains incompletely understood. Here, using genetically-engineered chicken DT40 cells, we first determined that Zrt/Irt-like protein (ZIP) transporters that are localized to the compartments of the early secretory pathway play only a minor role in the ALP activation process. These transporters included ZIP7, ZIP9, and ZIP13, performing pivotal functions in maintaining cellular homeostasis by effluxing zinc out of the compartments. Next, using purified ALP proteins, we showed that zinc metalation on ALP produced in DT40 cells lacking ZNT5–ZNT6 heterodimers and ZNT7 homodimers is impaired. Finally, by genetically disrupting both ZNT5 and ZNT7 in human HAP1 cells, we directly demonstrated that the tissue-nonspecific ALP-activating functions of both ZNT complexes are conserved in human cells. Furthermore, using mutant HAP1 cells, we uncovered a previously-unrecognized and unique spatial regulation of ZNT5–ZNT6 heterodimer formation, wherein ZNT5 recruits ZNT6 to the Golgi apparatus to form the heterodimeric complex. These findings fill in major gaps in our understanding of the molecular mechanisms underlying zinc ectoenzyme activation in the compartments of the early secretory pathway.

Formate oxidation to carbon dioxide is a key reaction in one-carbon compound metabolism, and its reverse reaction represents the first step in carbon assimilation in the acetogenic and methanogenic branches of many anaerobic organisms. The molybdenum-containing dehydrogenase FdsABG is a soluble NAD+-dependent formate dehydrogenase and a member of the NADH dehydrogenase superfamily. Here, we present the first structure of the FdsBG subcomplex of the cytosolic FdsABG formate dehydrogenase from the hydrogen-oxidizing bacterium Cupriavidus necator H16 both with and without bound NADH. The structures revealed that the two iron-sulfur clusters, Fe4S4 in FdsB and Fe2S2 in FdsG, are closer to the FMN than they are in other NADH dehydrogenases. Rapid kinetic studies and EPR measurements of rapid freeze-quenched samples of the NADH reduction of FdsBG identified a neutral flavin semiquinone, FMNH•, not previously observed to participate in NADH-mediated reduction of the FdsABG holoenzyme. We found that this semiquinone forms through the transfer of one electron from the fully reduced FMNH−, initially formed via NADH-mediated reduction, to the Fe2S2 cluster. This Fe2S2 cluster is not part of the on-path chain of iron-sulfur clusters connecting the FMN of FdsB with the active-site molybdenum center of FdsA. According to the NADH-bound structure, the nicotinamide ring stacks onto the re-face of the FMN. However, NADH binding significantly reduced the electron density for the isoalloxazine ring of FMN and induced a conformational change in residues of the FMN-binding pocket that display peptide-bond flipping upon NAD+ binding in proper NADH dehydrogenases.

7 May 2020 , Volume 96, Number 3

Systemic infection and proliferation of intracellular pathogens require the biogenesis of a growth-stimulating compartment. The gastrointestinal pathogen Salmonella enterica commonly forms highly dynamic and extensive tubular membrane compartments built from Salmonella-modified membranes (SMMs) in diverse host cells. Although the general mechanism involved in the formation of replication-permissive compartments of S. enterica is well researched, much less is known regarding specific adaptations to different host cell types. Using an affinity-based proteome approach, we explored the composition of SMMs in murine macrophages. The systematic characterization provides a broader landscape of host players to the maturation of Salmonella-containing compartments and reveals core host elements targeted by Salmonella in macrophages as well as epithelial cells. However, we also identified subtle host specific adaptations. Some of these observations, such as the differential involvement of the COPII system, Rab GTPases 2A, 8B, 11 and ER transport proteins Sec61 and Sec22B may explain cell line-dependent variations in the pathophysiology of Salmonella infections. In summary, our system-wide approach demonstrates a hitherto underappreciated impact of the host cell type in the formation of intracellular compartments by Salmonella.