Austin based sisters Tori, Tiffany and Sofia Baltierra have been playing as the Tiarra Girls since they were in elementary and middle school. Listen as they describe how seeing the video for No Doubt's "Just a Girl" influenced them all stylistically and helped them find their voices as young women in music.

Not weighed down by their 10-year drought at the Pac-12 tournament, the Cougars trailed for just 87 seconds against Colorado on Wednesday night before driving the Buffaloes into the ground, 82-68, at T-Mobile Arena.

The record breaking mega hit All About That Bass transformed Meghan Trainor into a chart-topping pop star before she turned 21 — but with sudden fame also came anxiety and self-doubt. Trainor put in the work to get back in a good place, the results of which can be heard on her new album, Treat Myself.

Pat O'Sullivan took over the Hillston Spectator from his dad more than 60 years ago. But now, with his garden calling, the publication could be at risk of folding.

The NSW Treasurer delivers a surplus of around half that expected this time last year, but is forecasting blue skies ahead. He's optimistic despite doubts circulating about the domestic and global economies, Ian Verrender says.

A war veteran in outback South Australia is saving war memorabilia from being discarded, sold online, and hidden away in storage.

The reliability of roadside drug testing for cannabis is in question after new research reveals a "concerning" proportion of the tests are inaccurate, while a driver argues the 'passive smoking' defence and has charges dismissed.

Four Northern Territory public service executives are being paid more than $200,000 a year to facilitate a planned gas industry that some experts doubt will ever stack up economically, particularly as terminals for importing gas from "wherever's cheapest" secure approval on the nation's east coast.

As bushfires continue across south-east Queensland, doubt is cast over this year's Riverfire event in Brisbane and whether fireworks have a place in today's society.

The former fastest-man-in-the-world makes his professional football debut for the Central Coast, but in 19 minutes he wasn't able to make much of an impact.

As the coronavirus spreads in Japan, the chief executive of the Tokyo Games said Friday he can’t guarantee the postponed Olympics will be staged next year -- even with the long delay.

The glass is always half-empty if you spilled the first-half down your shirt. HEY! FOLLOW @LAMEBOOK ON INSTAGRAM! THANKS!!

In an interview, Alex Zhu, the head of the Chinese video app TikTok, defends the company against accusations of spying and censorship and explains why he isn't interested in making the platform a place for political debate.

California's Fish and Game Commission on Wednesday gave emergency powers to Fish and Wildlife Department director Charlton Bonham, allowing him to suspend, delay or restrict recreational fishing in specific areas until May 31.

The author of "Sweetbitter" juggles child care and promoting her new L.A. area memoir, "Stray," reads poetry and takes solace in "The Office."

Rangers take on Kilmarnock in the Scottish Premiership on Wednesday night, with Steven Gerrard looking to keep within touching distance of rivals Celtic. Express Sport delivers you all of the team news for the Gers, as well as the predicted line up for the clash, as Ryan Jack returns from injury.

Arsene Wenger has praised Liverpool boss Jurgen Klopp after a stellar season in the Premier League.

Celtic boss Neil Lennon aimed a subtle dig at league rivals Rangers, after the Hoops went seven points clear at the top of the Scottish Premiership table following a 4-1 win over Hamilton Academical.

Arsene Wenger has praised Liverpool boss Jurgen Klopp after a stellar season in the Premier League.

(University of Maryland) Many historians have claimed the Justinianic Plague (c. 541-750 CE) killed half of the population of Byzantine (Eastern Roman) Empire. New historical research and mathematical modeling challenge the death rate and severity of this first plague pandemic, named for Emperor Justinian I.

When you are born, You know that you are, Here to change the world, And it is good, And it’s alright, To do your best. Then, You’re checking the curtains, Just in case, You run out of sunshine. -evocative short poetry-

When you are born, You know that you are, Here to change the world, And it is good, And it’s alright, To do your best. Then, You’re checking the curtains, Just in case, You run out of sunshine. -evocative short poetry-

Sagicor Finance Company has raised doubt about whether the deal to acquire the assets of Scotia Life Trinidad...

Approximately 11,500 unaccompanied children were apprehended at the U.S.-Mexico border in May, putting this year on track to exceed 2014's surge. As the U.S. government struggles to care for these child migrants, with public outrage mounting over reports of unsafe, filthy conditions in initial Border Patrol custody, the failure of the executive branch and Congress to plan for increased shelter and care demands are increasingly apparent, as this article explores.

The Paris-based International Energy Agency on Friday cast doubt on whether Russia would meet its agreement with OPEC to cut crude oil output to support prices.

London : Longmans, Green, 1903.

London : printed for Knight and Lacey, 1826.

Rockville, Maryland : National Institute on Drug Abuse, 1986.

Tree data are ubiquitous because they model a large variety of situations, e.g., the architecture of plants, the secondary structure of RNA, or the hierarchy of XML files. Nevertheless, the analysis of these non-Euclidean data is difficult per se. In this paper, we focus on the subtree kernel that is a convolution kernel for tree data introduced by Vishwanathan and Smola in the early 2000's. More precisely, we investigate the influence of the weight function from a theoretical perspective and in real data applications. We establish on a 2-classes stochastic model that the performance of the subtree kernel is improved when the weight of leaves vanishes, which motivates the definition of a new weight function, learned from the data and not fixed by the user as usually done. To this end, we define a unified framework for computing the subtree kernel from ordered or unordered trees, that is particularly suitable for tuning parameters. We show through eight real data classification problems the great efficiency of our approach, in particular for small data sets, which also states the high importance of the weight function. Finally, a visualization tool of the significant features is derived.

Este año en el mes en que se llevó a cabo el mantenimiento anual de Logos Hope en Uruguay, la tripulante Cecilia* de Argentina se unió a un pequeño equipo que sirve en Asia Central. Mientras estuvo allí, pudo asistir a dos iglesias subterráneas que desbordaban de esperanza y fe.

Last March, analysts and tech bloggers dumped out arrogant contempt over Apple's latest product introduction. This year, those new offerings helped save Apple's Q2 earnings and are projected to bolster its June quarter performance despite the pandemic.

Coatzacoalcos, Mexico :: Logos Hope's crewmembers encourage a youth group to listen to God's call to serve.

FIVE new nursery extensions being built at schools across Edinburgh have been thrown into doubt after the company building the facilities entered administration.

“We didn’t see any association between getting this medicine and the chance of dying or being intubated,” lead researcher Dr. Neil Schluger told Reuters in a telephone interview.

Presentation by Don Ball of Chemcut Corporation

Galatians 4:19–20

A.L. Sadler examines in minute detail the origins of tea drinking and the rich and complex components of its ritualization.

LIGO has explained how it processes gravitational wave data in greater detail than ever before. But some physicists still say the analysis contains mistakes

Title: Study Casts Doubt on Use of Common Antibiotic for UTIs in Women
Category: Health News
Created: 4/30/2015 12:00:00 AM
Last Editorial Review: 5/1/2015 12:00:00 AM

Title: Experts Cast Doubt on Notion That New Strain of Coronavirus Is More Infectious
Category: Health News
Created: 5/7/2020 12:00:00 AM
Last Editorial Review: 5/8/2020 12:00:00 AM

ABSTRACT

Anti-galactose-α-1,3-galactose (anti-α-Gal) antibody is naturally expressed at a high level in humans. It constitutes about 1% of immunoglobulins found in human blood. Here, we designed a live attenuated influenza virus vaccine that can generate α-Gal epitopes in infected cells in order to facilitate opsonization of infected cells, thereby enhancing vaccine-induced immune responses. In the presence of normal human sera, cells infected with this mutant can enhance phagocytosis of human macrophages and cytotoxicity of NK cells in vitro. Using a knockout mouse strain that allows expression of anti-α-Gal antibody in vivo, we showed that this strategy can increase vaccine immunogenicity and the breadth of protection. This vaccine can induce 100% protection against a lethal heterosubtypic group 1 (H5) or group 2 (mouse-adapted H3) influenza virus challenge in the mouse model. In contrast, its heterosubtypic protective effect in wild-type or knockout mice that do not have anti-α-Gal antibody expression is only partial, demonstrating that the enhanced vaccine-induced protection requires anti-α-Gal antibody upon vaccination. Anti-α-Gal-expressing knockout mice immunized with this vaccine produce robust humoral and cell-mediated responses upon a lethal virus challenge. This vaccine can stimulate CD11b^lo/– pulmonary dendritic cells, which are known to be crucial for clearance of influenza virus. Our approach provides a novel strategy for developing next-generation influenza virus vaccines.

IMPORTANCE Influenza A viruses have multiple HA subtypes that are antigenically diverse. Classical influenza virus vaccines are subtype specific, and they cannot induce satisfactory heterosubtypic immunity against multiple influenza virus subtypes. Here, we developed a live attenuated H1N1 influenza virus vaccine that allows the expression of α-Gal epitopes by infected cells. Anti-α-Gal antibody is naturally produced by humans. In the presence of this antibody, human cells infected with this experimental vaccine virus can enhance several antibody-mediated immune responses in vitro. Importantly, mice expressing anti-α-Gal antibody in vivo can be fully protected by this H1N1 vaccine against a lethal H5 or H3 virus challenge. Our work demonstrates a new strategy for using a single influenza virus strain to induce broadly cross-reactive immune responses against different influenza virus subtypes.

ABSTRACT

Bacillus subtilis contains two known cyclic di-GMP (c-di-GMP)-dependent receptors, YdaK and DgrA, as well as three diguanylate cyclases (DGCs): soluble DgcP and membrane-integral DgcK and DgcW. DgrA regulates motility, while YdaK is responsible for the formation of a putative exopolysaccharide, dependent on the activity of DgcK. Using single-molecule tracking, we show that a majority of DgcK molecules are statically positioned in the cell membrane but significantly less so in the absence of YdaK but more so upon overproduction of YdaK. The soluble domains of DgcK and of YdaK show a direct interaction in vitro, which depends on an intact I-site within the degenerated GGDEF domain of YdaK. These experiments suggest a direct handover of a second messenger at a single subcellular site. Interestingly, all three DGC proteins contribute toward downregulation of motility via the PilZ protein DgrA. Deletion of dgrA also affects the mobility of DgcK within the membrane and also that of DgcP, which arrests less often at the membrane in the absence of DgrA. Both, DgcK and DgcP interact with DgrA in vitro, showing that divergent as well as convergent direct connections exist between cyclases and their effector proteins. Automated determination of molecule numbers in live cells revealed that DgcK and DgcP are present at very low copy numbers of 6 or 25 per cell, respectively, such that for DgcK, a part of the cell population does not contain any DgcK molecule, rendering signaling via c-di-GMP extremely efficient.

IMPORTANCE Second messengers are free to diffuse through the cells and to activate all responsive elements. Cyclic di-GMP (c-di-GMP) signaling plays an important role in the determination of the life style transition between motility and sessility/biofilm formation but involves numerous distinct synthetases (diguanylate cyclases [DGCs]) or receptor pathways that appear to act in an independent manner. Using Bacillus subtilis as a model organism, we show that for two c-di-GMP pathways, DGCs and receptor molecules operate via direct interactions, where a synthesized dinucleotide appears to be directly used for the protein-protein interaction. We show that very few DGC molecules exist within cells; in the case of exopolysaccharide (EPS) formation via membrane protein DgcK, the DGC molecules act at a single site, setting up a single signaling pool within the cell membrane. Using single-molecule tracking, we show that the soluble DGC DgcP arrests at the cell membrane, interacting with its receptor, DgrA, which slows down motility. DgrA also directly binds to DgcK, showing that divergent as well as convergent modules exist in B. subtilis. Thus, local-pool signal transduction operates extremely efficiently and specifically.

ABSTRACT

A microfluidic system coupled with fluorescence microscopy is a powerful approach for quantitative analysis of bacterial growth. Here, we measure parameters of growth and dynamic localization of the cell division initiation protein FtsZ in Bacillus subtilis. Consistent with previous reports, we found that after division, FtsZ rings remain at the cell poles, and polar FtsZ ring disassembly coincides with rapid Z-ring accumulation at the midcell. In cells mutated for minD, however, the polar FtsZ rings persist indefinitely, suggesting that the primary function of the Min system is in Z-ring disassembly. The inability to recycle FtsZ monomers in the minD mutant results in the simultaneous maintenance of multiple Z-rings that are restricted by competition for newly synthesized FtsZ. Although the parameters of FtsZ dynamics change in the minD mutant, the overall cell division time remains the same, albeit with elongated cells necessary to accumulate a critical threshold amount of FtsZ for promoting medial division. Finally, the minD mutant characteristically produces minicells composed of polar peptidoglycan shown to be inert for remodeling in the wild type. Polar peptidoglycan, however, loses its inert character in the minD mutant, suggesting that the Min system not only is important for recycling FtsZ but also may have a secondary role in the spatiotemporal regulation of peptidoglycan remodeling.

IMPORTANCE Many bacteria grow and divide by binary fission in which a mother cell divides into two identical daughter cells. To produce two equally sized daughters, the division machinery, guided by FtsZ, must dynamically localize to the midcell each cell cycle. Here, we quantitatively analyzed FtsZ dynamics during growth and found that the Min system of Bacillus subtilis is essential to disassemble FtsZ rings after division. Moreover, a failure to efficiently recycle FtsZ results in an increase in cell size. Finally, we show that the Min system has an additional role in inhibiting cell wall turnover and contributes to the "inert" property of cell walls at the poles.