Pentagon press secretary said the Iranian-backed Houthis launched at least eight drones, five anti-ship ballistic missiles and three anti-ship cruise missiles at the USS Stockdale and the USS Spruance

Dyson returns to India with its latest no-heat damage hair styler and dryer, the Airstrait, in Delhi. Does it deliver on its promise?

Fire in shanty caused by battery explosion spreads to gas cylinder and injures youth, damaging multiple shanties

Humans are causing irreversible damage to the planet from burning fossil fuels, the biggest ever study of the available science concluded in a report designed to spur the fight against climate change.

Humans are causing irreversible damage to the planet from burning fossil fuels, the biggest ever study of the available science concluded in a report designed to spur the fight against climate change.

Libyan rebels have heavily attacked an airport in the capital Tripoli, killing six civilians and inflicting damage in and around the facility. Several civilians were also injured in the attack on Mitiga International Airport on Saturday, the country’s internationally-recognized government said. The Libyan Interior Ministry said at least 80 rockets were fired in the attack,...

Fernando Alonso is relying on tyre strategy to limit the damage to his narrow championship lead at tomorrow's Brazilian Grand Prix

Qantas has grounded three Boeing 737 jets after cracks were found in the part of the aircraft connecting the wings with the body. Maintenance engineers want the entire fleet grounded.

British boy bands Five, 911, Damage and A1 topped the charts and played sell-out concerts to thousands of screaming fans at the height of their fame - but the years since are a very different story.

Road users can rapidly find themselves out of pocket if their bike or vehicle is damaged by one, while cyclists can easily be injured if a pothole causes them to come off their bike.

With the continuing development of beamlines for macromolecular crystallography (MX) over the last few years providing ever higher X-ray flux densities, it has become even more important to be aware of the effects of radiation damage on the resulting structures. Nine papers in this issue cover a range of aspects related to the physics and chemistry of the manifestations of this damage, as observed in both MX and small-angle X-ray scattering (SAXS) on crystals, solutions and tissue samples. The reports include measurements of the heating caused by X-ray irradiation in ruby microcrystals, low-dose experiments examining damage rates as a function of incident X-ray energy up to 30 keV on a metallo-enzyme using a CdTe detector of high quantum efficiency as well as a theoretical analysis of the gains predicted in diffraction efficiency using these detectors, a SAXS examination of low-dose radiation exposure effects on the dissociation of a protein complex related to human health, theoretical calculations describing radiation chemistry pathways which aim to explain the specific structural damage widely observed in proteins, investigation of radiation-induced damage effects in a DNA crystal, a case study on a metallo-enzyme where structural movements thought to be mechanism related might actually be radiation-damage-induced changes, and finally a review describing what X-ray radiation-induced cysteine modifications can teach us about protein dynamics and catalysis. These papers, along with some other relevant literature published since the last Journal of Synchrotron Radiation Radiation Damage special issue in 2017, are briefly summarized below.

The mechanism of formation of residual strain in crystals with a damaged surface has been studied by transmission electron microscopy in GaAs wafers ground with sandpaper. The samples showed a dislocation network located near the sample surface penetrating to a depth of a few micrometres, comparable to the size of abrasive particles used for the treatment, and no other types of defects were observed. A simple model for the formation of a compressive strain induced by the dislocation network in the damaged layer is proposed, in satisfactory agreement with the measured strain. The strain is generated by the formation of dislocation half-loops at the crystal surface, having the same component of the Burgers vectors parallel to the surface of the crystal. This is equivalent to the insertion of extra half-planes from the crystal surface to the depth of the damaged zone. This model can be generalized for other crystal structures. An approximate calculation of the strain generated from the observed dislocation distribution in the sample agrees with the proposed model and permits the conclusion that this mechanism is in general sufficient to explain the observed compressive strain, without the need to consider other types of defects.

Traditionally small-molecule crystallographers have not usually observed or recognized significant radiation damage to their samples during diffraction experiments. However, the increased flux densities provided by third-generation synchrotrons have resulted in increasing numbers of observations of this phenomenon. The diversity of types of small-molecule systems means it is not yet possible to propose a general mechanism for their radiation-induced sample decay, however characterization of the effects will permit attempts to understand and mitigate it. Here, systematic experiments are reported on the effects that sample temperature and beam attenuation have on radiation damage progression, allowing qualitative and quantitative assessment of their impact on crystals of a small-molecule test sample. To allow inter-comparison of different measurements, radiation-damage metrics (diffraction-intensity decline, resolution fall-off, scaling B-factor increase) are plotted against the absorbed dose. For ease-of-dose calculations, the software developed for protein crystallography, RADDOSE-3D, has been modified for use in small-molecule crystallography. It is intended that these initial experiments will assist in establishing protocols for small-molecule crystallographers to optimize the diffraction signal from their samples prior to the onset of the deleterious effects of radiation damage.

Copper-containing nitrite reductases (CuNiRs) that convert NO2− to NO via a CuCAT–His–Cys–CuET proton-coupled redox system are of central importance in nitrogen-based energy metabolism. These metalloenzymes, like all redox enzymes, are very susceptible to radiation damage from the intense synchrotron-radiation X-rays that are used to obtain structures at high resolution. Understanding the chemistry that underpins the enzyme mechanisms in these systems requires resolutions of better than 2 Å. Here, for the first time, the damage-free structure of the resting state of one of the most studied CuNiRs was obtained by combining X-ray free-electron laser (XFEL) and neutron crystallography. This represents the first direct comparison of neutron and XFEL structural data for any protein. In addition, damage-free structures of the reduced and nitrite-bound forms have been obtained to high resolution from cryogenically maintained crystals by XFEL crystallography. It is demonstrated that AspCAT and HisCAT are deprotonated in the resting state of CuNiRs at pH values close to the optimum for activity. A bridging neutral water (D2O) is positioned with one deuteron directed towards AspCAT Oδ1 and one towards HisCAT N∊2. The catalytic T2Cu-ligated water (W1) can clearly be modelled as a neutral D2O molecule as opposed to D3O+ or OD−, which have previously been suggested as possible alternatives. The bridging water restricts the movement of the unprotonated AspCAT and is too distant to form a hydrogen bond to the O atom of the bound nitrite that interacts with AspCAT. Upon the binding of NO2− a proton is transferred from the bridging water to the Oδ2 atom of AspCAT, prompting electron transfer from T1Cu to T2Cu and reducing the catalytic redox centre. This triggers the transfer of a proton from AspCAT to the bound nitrite, enabling the reaction to proceed.

The local Fourier-space relation between diffracted intensity I, diffraction wavevector q and dose D, ilde I(q,D), is key to probing and understanding radiation damage by X-rays and energetic particles in both diffraction and imaging experiments. The models used in protein crystallography for the last 50 years provide good fits to experimental I(q) versus nominal dose data, but have unclear physical significance. More recently, a fit to diffraction and imaging experiments suggested that the maximum tolerable dose varies as q−1 or linearly with resolution. Here, it is shown that crystallographic data have been strongly perturbed by the effects of spatially nonuniform crystal irradiation and diffraction during data collection. Reanalysis shows that these data are consistent with a purely exponential local dose dependence, ilde I(q,D) = I0(q)exp[−D/De(q)], where De(q) ∝ qα with α ≃ 1.7. A physics-based model for radiation damage, in which damage events occurring at random locations within a sample each cause energy deposition and blurring of the electron density within a small volume, predicts this exponential variation with dose for all q values and a decay exponent α ≃ 2 in two and three dimensions, roughly consistent with both diffraction and imaging experiments over more than two orders of magnitude in resolution. The B-factor model used to account for radiation damage in crystallographic scaling programs is consistent with α = 2, but may not accurately capture the dose dependencies of structure factors under typical nonuniform illumination conditions. The strong q dependence of radiation-induced diffraction decays implies that the previously proposed 20–30 MGy dose limit for protein crystallography should be replaced by a resolution-dependent dose limit that, for atomic resolution data sets, will be much smaller. The results suggest that the physics underlying basic experimental trends in radiation damage at T ≃ 100 K is straightforward and universal. Deviations of the local I(q, D) from strictly exponential behavior may provide mechanistic insights, especially into the radiation-damage processes responsible for the greatly increased radiation sensitivity observed at T ≃ 300 K.

The 5.8-magnitude earthquake that shook the eastern United States on the afternoon of Tuesday, Aug. 23, caused minor damage to some of the Smithsonian's natural history collections. All public Smithsonian museums are open and have been determined safe for visitors and staff.

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Imke K. Mandemaker, Di Zhou, Serena T. Bruens, Dick H. Dekkers, Pernette J. Verschure, Raghu R. Edupuganti, Eran Meshorer, Jeroen A. Demmers, and Jurgen A. Marteijn

Many chromatin remodeling and modifying proteins are involved in the DNA damage response by stimulating repair or inducing DNA damage signaling. Interestingly, here we identified that down regulation of the H1-interacting protein SET results in increased resistance to a wide variety of DNA damaging agents. We found that this increased resistance is not the result of an inhibitory effect of SET on DNA repair, but rather the consequence of a suppressed apoptotic response to DNA damage. We further provide evidence that the histone chaperone SET is responsible for the eviction of H1 from chromatin. Knock down of H1 in SET-depleted cells resulted in re-sensitization of cells to DNA damage, suggesting that the increased DNA damage resistance in SET-depleted cells is the result of enhanced retention of H1 on chromatin. Finally, clonogenic survival assays show that SET and p53 are epistatic in attenuating DNA damage-induced cell death. Altogether, our data show a role for SET in the DNA damage response as a regulator of cell survival following genotoxic stress.

A team from the Smithsonian is starting a pilot program to aid people in restoring their damaged family heirlooms. Click photo to learn more….

The post Smithsonian starts program to help people restore storm-damaged heirlooms appeared first on Smithsonian Insider.

How do you diagnose a sick stream? Count its insects, according to Smithsonian biologist Don Weller.

The post Streams damaged by too many hard surfaces in urban areas appeared first on Smithsonian Insider.

The impact of polluted urban soil on trees is highlighted in a recent study from Latvia. The researchers found that high salt levels from de-icing chemicals and nutrient imbalance in soil damaged lime trees growing in the city of Riga.

Selective fishing aims to prevent the overexploitation of target fish species and to protect by-catch species, but recent research has indicated that it could be having the opposite effect by damaging biodiversity and sustainability. An alternative approach called 'balanced exploitation' works at the level of the ecosystem instead of selectively removing specific components from the ecosystem.

Between 10 and 50% of marine species and ecosystems are recovering from population declines and degradation, according to recent research, which identified five strategies for successful recoveries. Recoveries are often driven by a combination of factors, which include restricting exploitation, better protection of vulnerable habitats and greater political support and local involvement with conservation.

Some Marine and Coastal have been altered over long periods of time, resulting in a loss of knowledge of their true healthy state, new research suggests. In this UK study, researchers used historical records, samples of sediment and present-day diving surveys to reconstruct the true history of shellfish beds on the east coast of Scotland.

Bycatch (species caught unintentionally in fisheries) in some commercial marine fisheries is the main cause of death in some populations of seabirds, sea turtles, marine mammals, sharks and fish. New research has revealed that bycatch from tuna fishing can be reduced by using better equipment, without compromising industry profits. However, significant policy efforts are needed to monitor bycatch and enforce more sustainable fishing practices.

The impact of polluted urban soil on trees is highlighted in a recent study from Latvia. The researchers found that high salt levels from de-icing chemicals and nutrient imbalance in soil damaged lime trees growing in the city of Riga.

Damage caused by boats anchoring in seagrass meadows off the coast of Sardinia continues despite restrictions, new research shows. The study's authors provide a number of recommendations to help protect seagrass. These include creating special anchoring areas in seagrass-free locations, and limiting the number of boats that enter a marine protected area.

A new method of assessing human impacts on seafloor habitats suggests that over a third of habitats in the Baltic Sea have an ‘unfavourable’ status. The method is presented in a recent study which concludes that the tool can be effective in helping implement the EU’s Marine Strategy Framework Directive (MSFD).

During the last century, large areas of the seafloor have been damaged by human activities such as fishing, sand and gravel extraction and navigational dredging. This Thematic Issue presents quality research exploring the physical damage to the seafloor, new methods for providing seabed information and pressures on marine ecosystems from activities such as deep-sea mining.

Sustainability in the production of goods and services could be encouraged by replacing value-added tax (VAT) with ‘DaVAT,’ a damage and value-added tax, a new study suggests. This tariff is partly based on a life-cycle assessment (LCA) of goods and services and varies from high (products deemed to seriously harm the environment and human health) to low (those with a lesser impact). The researchers propose a novel way to convert VAT into DaVAT and provide a new policy tool, based on LCA, that can be applied by any country wishing to reform its consumption tax system and move towards a more sustainable future.

Higher atmospheric carbon dioxide levels and temperatures under climate change are likely to increase the rate of corrosive damage in reinforced concrete structures, according to a recent study. This could result in costly repairs in the future, unless structures are suitably adapted.

Over 75 000 regulatory inspection reports for over 32 000 oil and gas production wells drilled in Pennsylvania, US between 2000 and 2012, have been analysed in a recent study. In these reports, the inspectors logged six times as many incidents of damage to the walls of shale gas and oil wells than in wells for conventional oil and gas.

Ocean acidification could damage the organs of Atlantic herring, as well as slow their growth and development, recent experiments show. It adds to the list of pressures currently threatening this commercially important species, including over-fishing and marine pollution.

Marine litter not only causes environmental damage, but has significant economic costs for industry. A recent study has now estimated that marine litter in the Asia-Pacific region is likely to cost over US$1.26billion per year in damage to marine industries. Policy options for reducing this cost are explored.

Human activities are threatening river ecosystems in the Mediterranean. Recent research in south-east Spain has highlighted the need to assess biodiversity and the ecological condition of river ecosystems at both basin-wide and local scales. The researchers say this will provide a better assessment of river ecosystems, aiding management decisions.

Climate change is likely to increase the frequency and severity of flooding. This study reviewed damage mitigating measures at local, regional and national scales, and suggests that approaches including both spatial planning and private precautionary measures (such as building adaptations) are important for integrated risk management.

Researchers have developed a new tool for assessing and predicting the damage caused by droughts to crop yields and hydroelectric energy production. The tool could provide useful information to policymakers, helping them develop drought management practices to improve food and energy security and adapt to climate change.

New research from the Mediterranean suggests that marine ecosystems are disturbed by the organic food and faeces waste from fish farms, even when the pollutants themselves can no longer be detected. The study recommends calculating an individual pollution 'carrying capacity' for each type of marine habitat, and provides the first published estimate for a ma??rl habitat.

Adapted habitat equivalency analysis (HEA) may help decision makers select projects to restore damaged ecosystems under a limited budget. HEA, used to assess damages to natural resources, can help to clarify objectives and compare trade-offs between projects to choose the most cost-effective among them, according to this study’s authors.

Insect-eating bats are estimated to be worth US$ 1 billion (€0.91 billion) a year to maize farmers around the world, a new study has revealed. Not only do bats reduce crop damage by eating adult corn earworm crop pests, they also suppress fungal infections in maize ears. Bats and their habitats need to be better protected for their ecological and economic contributions, say the study’s authors.

Moss helps prevent soil erosion on fire-damaged land, a Portuguese study suggests. The researchers analysed water that ran off from post-fire hillsides. They found less sediment and organic matter in run-off from patches of land with high levels of moss than from those with low levels of moss. Moss quickly establishes itself on land after fires, so the study suggests that land managers could take advantage of its restorative effects by deliberately encouraging its growth.

Thousands of tonnes of chemical warfare agents were dumped into the Baltic Sea after the Second World War. A recent study has shown that fish caught near the dumping grounds show high levels of genetic and cell damage, revealing the long legacy of these toxic substances.

Nano-sized particles of plastic can be more damaging to marine species than larger sized microplastics, a new study shows. Lab tests revealed that nanoplastics can damage cell membranes in tiny marine creatures called rotifers (Rotifera), disrupting their natural defences against toxicants. The researchers found that rotifers that had been exposed to nanoparticles of polystyrene were significantly more susceptible to the lethal effects of persistent organic pollutants (POPs).