Sněmovna schválila návrh zákona o některých opatřeních ke zmírnění dopadů epidemie koronaviru. Jaké úlevy se dočkají dlužníci a jaké jsou podmínky pro zastavení exekuce, vysvětluje právník Adam Stawaritsch.

Near Paris, there is no need to see if there is another car behind you...

Minecraft or as I prefer to call it “Minecrack” is a game that was recently recommend to my by a friend. At first glance the game looks like a throwback to the original Atari or Nintendo game systems (think Mario Bros type graphics minus having to jump on walking mushrooms) The game essentially has the look of a giant Lego world consisting entirely of blocks, the animals, trees, even the water are all blocks. There’s also no set point system or objective you have to reach in order to beat the game. But despite this the game is exceptionally addictive. I personally found it very easy to spend a few hours on it before I realized how much time had gone by.

For those eagerly waiting for the first half of the first half of the Mincraft adventure update, the wait is finally over. And no I’m not talking about some buggy pre release that is a pain in the ass to properly install that’s been out for just over a week prior. As of Sept 14 it’s available to the masses who are looking to feed their “minecrack” addiction........... Because of the recent changes I figured it was time for an updated Minecraft review covering some of the recent changes. This is not a complete review on the 1.8 adventure update, just what I’ve seen and experienced in the game so far to give you an idea what to expect..........

With November being scheduled as the “official” release of Minecraft the team at Mojang are hard at work putting together Minecraft’s 1.9 update. This is possibly the last update before the release and like the 1.8 adventure update will be packed full of new features, crafting recipes and other stuff. Details at this point are scarce, but from the information that’s out there here are some of the things that could be included in the new 1.9 update..........

Wondering when Minecraft’s 1.9 update is coming out, very soon. Very soon as in how long it takes to download the pre release just released today. Or if you prefer to wait for a non buggy version like 1.8 it should be out in a few days once the team at Mojang get player feedback to fix potential bugs...........

Like Minecraft but looking for something a little different? Recently PC Gamer briefly reviewed a new game called Blockade Runner (Click here to see the PC Gamer review) essentially bringing something like Minecraft into space. Players currently are able to build their own spaceships, or space stations from scratch in much the same way Minecraft’s creative mode works, but in zero g. Unlike Minecraft however (unless your using a mod) you can attach different weapons to your creations and fly them around, blasting other ships and asteroids to pieces........................

Unpacking the Wuhan connection to Victoria's biggest COVID-19 cluster  The AgeView Full coverage on Google News

It's also perfect for last-minute gifting. READ MORE...

Last week, my teenage son came to me and said, “Yo, Mom. Can we get some baby ducks?” No joke. He really said “Yo, Mom.” (Cue the eye roll.) And he really asked for ducks. (Cue the eyebrow lift.) I quickly brushed off this request

If we do some digging into the revision archives of the National Electrical Code (NEC), we can pretty much trace every requirement to one thing: saving lives! That is why the NEC exists; its purpose, the practical safeguarding of persons and property

The National Fire Protection Association (NFPA) has released a free 1st Responder Connection App offering firefighters, EMS, command staff, public educators, wildland fire personnel, and authorities having jurisdiction (AHJs) convenient, digital access to best practices, safety tips, and emergency response content.

Tucked into the foothills of California's beloved Laurel Canyon, the Laurel Hills Residence by Assembledge+ is an exquisite example of modern, sustainable home design. Comprised of three cedar-clad volumes with massive glass panels, the house features interconnecting pavilions that are topped with a broad overhangs to shield the interior spaces from extensive sun exposure.[...]

Want to recycle an old map and create a holiday gift at the same time? Why not try out Phizzychick’s tutorial for a Map Paper Pinecone. These beautiful faux pinecones can be used as ornaments, part of a garland or placed in a lovely seasonal dish. They are perfect for both fall and winter decor. […]

There's no evidence that Bill Gates ever visited Jeffrey Epstein's private island in the Caribbean, contrary to a viral Facebook post claiming he went there numerous times. The "flight records" cited do not show that.

The post False Claim Targets Gates Using Epstein Connection appeared first on FactCheck.org.

Https is an important part of keeping your data safe, but it's only a part. It's important to understand what it does and doesn't mean.

Are HTTPS Connections Really Safe? from Ask Leo!.
Get the newsletter: https://newsletter.askleo.com

An animation supervisor and a senior animator at Framestore discuss the challenges — and surprising upsides — of working remotely.

The post Coronavirus Stories: How Animators On The Upcoming ‘Tom And Jerry’ Feature Are Staying Connected appeared first on Cartoon Brew.

Facebook Gaming welcomes you to a new world of play. Share in the joy and fun of rooting for top streamers as they play the world’s biggest games.

Here, you can play games instantly, and join groups centered around everything you are interested The ways to have fun are

The butterfly counts not months but moments and has time enough Rabindranath Tagore one of my favourites. I saw the first butterfly of the season today . A Cabbage White the scourge of gardeners who love their cabbages . The pretty white things lays

Did you know that there was a connection between our large 7000 inhabitant village of Wingerworth Randolph Hearst and the a museum across the pond in St Louis No neither did I until I treated our village as if I were a visitor on a first visit . As a v

Aaron Weinreb, a 48-year-old OB-GYN, has been in home detention on $1.5 million bail since his arrest in October on charges he used the gay dating app Grindr to connect with an underage boy for sex.Because they’ve found a second victim, prosecutors wantED Weinreb jailed while he awaits trial.

Clippers forward Patrick Patterson, who is working toward becoming a film producer, is spending part of the shutdown hosting online movie nights.

Athletes, entertainers and others have an alternative way to connect with fans thanks to their cellphone and Cameo, a social media company.

For Times staff writer Catherine Saillant, an article led to a reader email, which led to a family reunion of sorts.

NHL players Logan Couture, Marc-Andre Fleury, Anze Kopitar and Ryan Getzlaf find plenty of time to show off on video chat amid the COVID-19 pandemic.

America is in the midst of a loneliness epidemic. Here, we offer 20 ways to combat social isolation and loneliness in the new year.

Anger at Xi Jinping's government over the coronavirus crisis is warranted, but treating a nuclear and economic superpower as an existential enemy to satisfy domestic political needs isn't the smart way to go.

Highland Park resident Olivia Arthur calls it "art for social distancing — a drive-by gallery for neighbors." The goal: Bring some joy to quarantine.

Hundreds of tango lovers unite on Zoom for the Earth Virtual Milonga. Some dance as couples. Some dance with a pillow. At this party it's all good.

A book club for people who don't like book clubs, founded in 2012 in San Francisco and now boasting six chapters in L.A. County, has moved online.

The Oscar-nominated animation shorts often share emotional themes, but they are a creatively diverse collection.

Indianapolis Prize finalist Sylvia Earle was the first woman to act as an Explorer in Residence for the National Geographic Society.

Where's the beef? In California, it is still available, but meat eaters might need to settle for cuts other than prime steaks. The industry is dealing with production backups caused by coronavirus pandemic.

You can easily connect external storage drives to iPad and iPhone, providing easy access to all the files and data on those drives via the Files app. This includes connecting flash drives, USB hard drives, SD cards, and just about any other storage medium to iPhone, iPad Pro, and iPad you can think of, as ... Read More

Did you know that, on average, 51% of companies are currently using marketing automation, and more than half of B2B companies (58%) plan to adopt the technology? The statistic may be mind-boggling, but it is very much justified. Companies and marketers are always in search of ways that make things easy and convenient for them. Automating […]

The post Marketing Automation and Customer Service: How are they Connected? appeared first on ReadWrite.

The Amateur Traveler talks to Jen Russell about the 3rd smallest state in the U.S. Connecticut. Connecticut, the Constitution State, is located halfway between New York and Boston. It has a coastline sheltered by Long Island so more suitable for family frolicking than for surfing. Connecticut also has a long maritime tradition which is celebrated at Mystic Seaport. It's forested rolling hills are popular with people who come to New England in the Fall to see the Fall colors. Connecticut is rich in U.S. history producing people such Revolutionary War patriot Nathan Hale ("My only regret is that I have but one life to give to my country"). It is rich in natural history as evidenced by Dinosaur State Park.

Chatham House and Routledge, Taylor & Francis are launching the Journal of Cyber Policy on 2 July.

The arrangement of functionally-related genes in operons is a fundamental element of how genetic information is organized in prokaryotes. This organization ensures coordinated gene expression by co-transcription. Often, however, alternative genetic responses to specific stress conditions demand the discoordination of operon expression. During cold temperature stress, accumulation of the gene encoding the sole Asp–Glu–Ala–Asp (DEAD)-box RNA helicase in Synechocystis sp. PCC 6803, crhR (slr0083), increases 15-fold. Here, we show that crhR is expressed from a dicistronic operon with the methylthiotransferase rimO/miaB (slr0082) gene, followed by rapid processing of the operon transcript into two monocistronic mRNAs. This cleavage event is required for and results in destabilization of the rimO transcript. Results from secondary structure modeling and analysis of RNase E cleavage of the rimO–crhR transcript in vitro suggested that CrhR plays a role in enhancing the rate of the processing in an auto-regulatory manner. Moreover, two putative small RNAs are generated from additional processing, degradation, or both of the rimO transcript. These results suggest a role for the bacterial RNA helicase CrhR in RNase E-dependent mRNA processing in Synechocystis and expand the known range of organisms possessing small RNAs derived from processing of mRNA transcripts.

Formate oxidation to carbon dioxide is a key reaction in one-carbon compound metabolism, and its reverse reaction represents the first step in carbon assimilation in the acetogenic and methanogenic branches of many anaerobic organisms. The molybdenum-containing dehydrogenase FdsABG is a soluble NAD+-dependent formate dehydrogenase and a member of the NADH dehydrogenase superfamily. Here, we present the first structure of the FdsBG subcomplex of the cytosolic FdsABG formate dehydrogenase from the hydrogen-oxidizing bacterium Cupriavidus necator H16 both with and without bound NADH. The structures revealed that the two iron-sulfur clusters, Fe4S4 in FdsB and Fe2S2 in FdsG, are closer to the FMN than they are in other NADH dehydrogenases. Rapid kinetic studies and EPR measurements of rapid freeze-quenched samples of the NADH reduction of FdsBG identified a neutral flavin semiquinone, FMNH•, not previously observed to participate in NADH-mediated reduction of the FdsABG holoenzyme. We found that this semiquinone forms through the transfer of one electron from the fully reduced FMNH−, initially formed via NADH-mediated reduction, to the Fe2S2 cluster. This Fe2S2 cluster is not part of the on-path chain of iron-sulfur clusters connecting the FMN of FdsB with the active-site molybdenum center of FdsA. According to the NADH-bound structure, the nicotinamide ring stacks onto the re-face of the FMN. However, NADH binding significantly reduced the electron density for the isoalloxazine ring of FMN and induced a conformational change in residues of the FMN-binding pocket that display peptide-bond flipping upon NAD+ binding in proper NADH dehydrogenases.

Invitation Only Research Event

Event participants

Formate oxidation to carbon dioxide is a key reaction in one-carbon compound metabolism, and its reverse reaction represents the first step in carbon assimilation in the acetogenic and methanogenic branches of many anaerobic organisms. The molybdenum-containing dehydrogenase FdsABG is a soluble NAD+-dependent formate dehydrogenase and a member of the NADH dehydrogenase superfamily. Here, we present the first structure of the FdsBG subcomplex of the cytosolic FdsABG formate dehydrogenase from the hydrogen-oxidizing bacterium Cupriavidus necator H16 both with and without bound NADH. The structures revealed that the two iron-sulfur clusters, Fe4S4 in FdsB and Fe2S2 in FdsG, are closer to the FMN than they are in other NADH dehydrogenases. Rapid kinetic studies and EPR measurements of rapid freeze-quenched samples of the NADH reduction of FdsBG identified a neutral flavin semiquinone, FMNH•, not previously observed to participate in NADH-mediated reduction of the FdsABG holoenzyme. We found that this semiquinone forms through the transfer of one electron from the fully reduced FMNH−, initially formed via NADH-mediated reduction, to the Fe2S2 cluster. This Fe2S2 cluster is not part of the on-path chain of iron-sulfur clusters connecting the FMN of FdsB with the active-site molybdenum center of FdsA. According to the NADH-bound structure, the nicotinamide ring stacks onto the re-face of the FMN. However, NADH binding significantly reduced the electron density for the isoalloxazine ring of FMN and induced a conformational change in residues of the FMN-binding pocket that display peptide-bond flipping upon NAD+ binding in proper NADH dehydrogenases.

Invitation Only Research Event

Lipid rafts are highly ordered regions of the plasma membrane that are enriched in cholesterol and sphingolipids and play important roles in many cells. In hematopoietic stem and progenitor cells (HSPCs), lipid rafts house receptors critical for normal hematopoiesis. Lipid rafts also can bind and sequester kinases that induce negative feedback pathways to limit proliferative cytokine receptor cycling back to the cell membrane. Modulation of lipid rafts occurs through an array of mechanisms, with optimal cholesterol efflux one of the major regulators. As such, cholesterol homeostasis also regulates hematopoiesis. Increased lipid raft content, which occurs in response to changes in cholesterol efflux in the membrane, can result in prolonged receptor occupancy in the cell membrane and enhanced signaling. In addition, certain diseases, like diabetes, may contribute to lipid raft formation and affect cholesterol retention in rafts. In this review, we explore the role of lipid raft-related mechanisms in hematopoiesis and CVD (specifically, atherosclerosis) and discuss how defective cholesterol efflux pathways in HSPCs contribute to expansion of lipid rafts, thereby promoting myelopoiesis and thrombopoiesis. We also discuss the utility of cholesterol acceptors in contributing to lipid raft regulation and disruption, and highlight the potential to manipulate these pathways for therapeutic gain in CVD as well as other disorders with aberrant hematopoiesis.

The arrangement of functionally-related genes in operons is a fundamental element of how genetic information is organized in prokaryotes. This organization ensures coordinated gene expression by co-transcription. Often, however, alternative genetic responses to specific stress conditions demand the discoordination of operon expression. During cold temperature stress, accumulation of the gene encoding the sole Asp–Glu–Ala–Asp (DEAD)-box RNA helicase in Synechocystis sp. PCC 6803, crhR (slr0083), increases 15-fold. Here, we show that crhR is expressed from a dicistronic operon with the methylthiotransferase rimO/miaB (slr0082) gene, followed by rapid processing of the operon transcript into two monocistronic mRNAs. This cleavage event is required for and results in destabilization of the rimO transcript. Results from secondary structure modeling and analysis of RNase E cleavage of the rimO–crhR transcript in vitro suggested that CrhR plays a role in enhancing the rate of the processing in an auto-regulatory manner. Moreover, two putative small RNAs are generated from additional processing, degradation, or both of the rimO transcript. These results suggest a role for the bacterial RNA helicase CrhR in RNase E-dependent mRNA processing in Synechocystis and expand the known range of organisms possessing small RNAs derived from processing of mRNA transcripts.

Publication Date: Apr 10 2020 New Fund Launches In addition to completing the Listing Application, new funds are required to complete a certification prior to receiving approval of an initial listing application. The certification can be found here. Listing Transfers In addition to completing the Listing Application, funds switching from another market to Nasdaq are required to complete a certification regarding compliance with SEC Rule 6c-11. The certification must be completed prior to...