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Hair we go again. Sorry, Paper Monitor couldn't resist.

Yes, it's another hair story, and yes, there's a picture of Jennifer Aniston.

This time, however, the Daily Mail reports that the Friends star has finally fallen out of favour. At least, her hairstyle has anyway.

It says a survey on the best onscreen hairstyles reveals her locks are no longer the most influential.

"Sorry, Jen... Anne's top of the crops," is its headline, revealing that Anne Hathaway's crowning glory has outshone the competition.

The elfin cut was first sported in the 2011 adaptation of David Nicholls's hit novel One Day. But it was her Oscar-winning turn in Les Miserables, as Fantine, which saw her cut it off for an extended period.

The actress was said to be "inconsolable" after the chop so it's quite a turnaround.

For those interested in which other celebrities made the cut, Miss Aniston's long curly style in Along Came Polly was in second place. And Audrey Hepburn's "up do" from 1963 film Charade in third.

Frank Reich has worked hard to find ways to teach the Colts virtually, including bringing in an NBA legend to inspire the team.

At Swoop we use Redis extensively for caching, message processing and analytics. The Redis documentation can be pithy at times and recently I found myself wanting to look in more depth at the Redis wire protocol. Getting everything set up … Continue reading →

Frank Reich has worked hard to find ways to teach the Colts virtually, including bringing in an NBA legend to inspire the team.

• Dockworkers in Belgium are wearing bracelets to enforce social distancing.
• The bracelets were already used to detect if someone fell into the water, but now they will sound an alarm if workers get to close to each other.
• Manufacturers say there is no privacy issue and the bracelets don't track workers' locations, despite concerns.
• Visit Business Insider's homepage for more stories.

Quarantine and social distancing are going high-tech as countries and companies embrace wearables. In Antwerp, Belgium, dockworkers are instructed to wear bracelets that enforce social distancing rules while they work.

Europe, where more than 100,000 people have died from COVID-19, is slowly starting to reopen in some places. Stay at home orders are expiring in many countries, while nonessential travel has stopped across the EU, and countries look towards the summer to anticipate what kind of travel might be possible. 

People are beginning to go back to work, which in some sectors means inevitable close contact, especially in many essential jobs. Social distancing bracelets in Belgium are one idea bing tested to see what the future of work might look like after coronavirus.

Here's how it works. 

SEE ALSO: People arriving in Hong Kong must wear tracking bracelets for 2 weeks or face jail time. Here's how they work.

The black, plastic bracelets are worn on the wrist like a watch.

They're made by Belgian company Rombit, which says that they are "a fully integrated personal safety and security device, specifically designed for highly industrial environments."

Source: Romware

Rombit already made bracelets useful in the port setting, which could be used to call for help if a worker fell into the water or another accident occurred.

Europe is slowly starting to go back to work, but fears of a second wave are making officials cautious.

Contact tracing is one solution being explored around the world, and the manufacturers of the bracelet believe it could also be used for contact tracing.

Source: The Associated Press

European health guidances say to wash hands, wear masks, and keep at least 1.5 meters, or about five feet, apart.

When two workers are less than five feet apart, the bracelets will sound warnings.

Rombit CEO John Baekelmans told Reuters that the bracelets won't allow companies to track employees' locations, because the devices are only connected to each other. He says there is no central server.

Source: Reuters

Workers in the control tower will be the first to test the bracelets early this month.

Then, the Port of Antwerp will likely expand the devices to tug boat workers.

Baekelmans told Reuters that Rombit already had hundreds of requests in 99 countries, and is hoping to ramp up production to 25,000 in a few weeks.

The former scandal figure is mining what was a traumatic experience for some dark humor.

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Wellness is a buzzy word lately. Over the past few years, we’ve seen a surge in all things health, wellness, and spirituality. Juice bars are popping up, boutique studios are becoming more accessible, and essential oils are chilling us out. Self-care is becoming more of the norm too, but amidst all this hype, it can be challenging to find the right routine that promotes good exercise, sleep, vitals, and mindset.  Read more...

Amelia C. Peterson
Nov 1, 2012; 11:1475-1488
Technological Innovation and Resources

Leigh Anderson
Apr 1, 2006; 5:573-588
Research

Actinobacillus pleuropneumoniae (App) is the etiological agent of acute porcine pneumonia and responsible for severe economic losses worldwide. The capsule polymer of App serotype 1 (App1) consists of [4)-GlcNAc-β(1,6)-Gal-α-1-(PO4-] repeating units that are O-acetylated at O-6 of the GlcNAc. It is a major virulence factor and was used in previous studies in the successful generation of an experimental glycoconjugate vaccine. However, the application of glycoconjugate vaccines in the animal health sector is limited, presumably because of the high costs associated with harvesting the polymer from pathogen culture. Consequently, here we exploited the capsule polymerase Cps1B of App1 as an in vitro synthesis tool and an alternative for capsule polymer provision. Cps1B consists of two catalytic domains, as well as a domain rich in tetratricopeptide repeats (TPRs). We compared the elongation mechanism of Cps1B with that of a ΔTPR truncation (Cps1B-ΔTPR). Interestingly, the product profiles displayed by Cps1B suggested processive elongation of the nascent polymer, whereas Cps1B-ΔTPR appeared to work in a more distributive manner. The dispersity of the synthesized products could be reduced by generating single-action transferases and immobilizing them on individual columns, separating the two catalytic activities. Furthermore, we identified the O-acetyltransferase Cps1D of App1 and used it to modify the polymers produced by Cps1B. Two-dimensional NMR analyses of the products revealed O-acetylation levels identical to those of polymer harvested from App1 culture supernatants. In conclusion, we have established a protocol for the pathogen-free in vitro synthesis of tailored, nature-identical App1 capsule polymers.

Kamen G. Ivanov and Pencho Petrushev
Trans. Amer. Math. Soc. 373 (2020), 3117-3176.
Abstract, references and article information

Actinobacillus pleuropneumoniae (App) is the etiological agent of acute porcine pneumonia and responsible for severe economic losses worldwide. The capsule polymer of App serotype 1 (App1) consists of [4)-GlcNAc-β(1,6)-Gal-α-1-(PO4-] repeating units that are O-acetylated at O-6 of the GlcNAc. It is a major virulence factor and was used in previous studies in the successful generation of an experimental glycoconjugate vaccine. However, the application of glycoconjugate vaccines in the animal health sector is limited, presumably because of the high costs associated with harvesting the polymer from pathogen culture. Consequently, here we exploited the capsule polymerase Cps1B of App1 as an in vitro synthesis tool and an alternative for capsule polymer provision. Cps1B consists of two catalytic domains, as well as a domain rich in tetratricopeptide repeats (TPRs). We compared the elongation mechanism of Cps1B with that of a ΔTPR truncation (Cps1B-ΔTPR). Interestingly, the product profiles displayed by Cps1B suggested processive elongation of the nascent polymer, whereas Cps1B-ΔTPR appeared to work in a more distributive manner. The dispersity of the synthesized products could be reduced by generating single-action transferases and immobilizing them on individual columns, separating the two catalytic activities. Furthermore, we identified the O-acetyltransferase Cps1D of App1 and used it to modify the polymers produced by Cps1B. Two-dimensional NMR analyses of the products revealed O-acetylation levels identical to those of polymer harvested from App1 culture supernatants. In conclusion, we have established a protocol for the pathogen-free in vitro synthesis of tailored, nature-identical App1 capsule polymers.

Problems might occur when using SAS Enterprise Guide with dual monitors. For example, it might appear there is a performance problem with the query builder or other task, or it might appear that code or a task is hung, or

Shwetha K. Shetty
Apr 1, 2020; 61:546-559
Methods

Protein tyrosine kinases (PTKs) play key roles in cellular signal transduction, cell cycle regulation, cell division, and cell differentiation. Dysregulation of PTK-activated pathways, often by receptor overexpression, gene amplification, or genetic mutation, is a causal factor underlying numerous cancers. In this study, we have developed a parallel reaction monitoring (PRM)-based assay for quantitative profiling of 83 PTKs. The assay detects 308 proteotypic peptides from 54 receptor tyrosine kinases and 29 nonreceptor tyrosine kinases in a single run. Quantitative comparisons were based on the labeled reference peptide method. We implemented the assay in four cell models: 1) a comparison of proliferating versus epidermal growth factor (EGF)-stimulated A431 cells, 2) a comparison of SW480Null (mutant APC) and SW480APC (APC restored) colon tumor cell lines, and 3) a comparison of 10 colorectal cancer cell lines with different genomic abnormalities, and 4) lung cancer cell lines with either susceptibility (11-18) or acquired resistance (11-18R) to the epidermal growth factor receptor tyrosine kinase inhibitor erlotinib. We observed distinct PTK expression changes that were induced by stimuli, genomic features or drug resistance, which were consistent with previous reports. However, most of the measured expression differences were novel observations. For example, acquired resistance to erlotinib in the 11-18 cell model was associated not only with previously reported upregulation of MET, but also with upregulation of FLK2 and downregulation of LYN and PTK7. Immunoblot analyses and shotgun proteomics data were highly consistent with PRM data. Multiplexed PRM assays provide a targeted, systems-level profiling approach to evaluate cancer-related proteotypes and adaptations. Data are available through Proteome eXchange Accession PXD002706.

Niemann-Pick disease type C (NPC) disease is a lipid-storage disorder that is caused by mutations in the genes encoding NPC proteins and results in lysosomal cholesterol accumulation. 2-Hydroxypropyl-β-cyclodextrin (CD) has been shown to reduce lysosomal cholesterol levels and enhance sterol homeostatic responses, but CD’s mechanism of action remains unknown. Recent work provides evidence that CD stimulates lysosomal exocytosis, raising the possibility that lysosomal cholesterol is released in exosomes. However, therapeutic concentrations of CD do not alter total cellular cholesterol, and cholesterol homeostatic responses at the ER are most consistent with increased ER membrane cholesterol. To address these disparate findings, here we used stable isotope labeling to track the movement of lipoprotein cholesterol cargo in response to CD in NPC1-deficient U2OS cells. Although released cholesterol was detectable, it was not associated with extracellular vesicles. Rather, we demonstrate that lysosomal cholesterol trafficks to the plasma membrane (PM), where it exchanges with lipoprotein-bound cholesterol in a CD-dependent manner. We found that in the absence of suitable extracellular cholesterol acceptors, cholesterol exchange is abrogated, cholesterol accumulates in the PM, and reesterification at the ER is increased. These results support a model in which CD promotes intracellular redistribution of lysosomal cholesterol, but not cholesterol exocytosis or efflux, during the restoration of cholesterol homeostatic responses.

The hydrolysis of triglycerides in triglyceride-rich lipoproteins by LPL is critical for the delivery of triglyceride-derived fatty acids to tissues, including heart, skeletal muscle, and adipose tissues. Physiologically active LPL is normally bound to the endothelial cell protein glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1 (GPIHBP1), which transports LPL across endothelial cells, anchors LPL to the vascular wall, and stabilizes LPL activity. Disruption of LPL-GPIHBP1 binding significantly alters triglyceride metabolism and lipid partitioning. In this study, we modified the NanoLuc® Binary Technology split-luciferase system to develop a novel assay that monitors the binding of LPL to GPIHBP1 on endothelial cells in real time. We validated the specificity and sensitivity of the assay using endothelial lipase and a mutant version of LPL and found that this assay reliably and specifically detected the interaction between LPL and GPIHBP1. We then interrogated various endogenous and exogenous inhibitors of LPL-mediated lipolysis for their ability to disrupt the binding of LPL to GPIHBP1. We found that angiopoietin-like (ANGPTL)4 and ANGPTL3-ANGPTL8 complexes disrupted the interactions of LPL and GPIHBP1, whereas the exogenous LPL blockers we tested (tyloxapol, poloxamer-407, and tetrahydrolipstatin) did not. We also found that chylomicrons could dissociate LPL from GPIHBP1 and found evidence that this dissociation was mediated in part by the fatty acids produced by lipolysis. These results demonstrate the ability of this assay to monitor LPL-GPIHBP1 binding and to probe how various agents influence this important complex.

Monique Lynch is a 29-year-old who believes her purpose is to improve the lives of those around her, especially the downtrodden and persons living with mental health issues. This angel-on-earth in the eyes of many is a Jill of several trades as...

Helen Macdonald and Carl Heneghan are back again talking about what's happened in the world of evidence this month. They start by talking about how difficult a task it is to find evidence that's definitely practice changing, what GPs can learn from Malawian children with nonsevere fast-breathing pneumonia, how radiation dosage varies...

For the next few months Talk Evidence is going to focus on the new corona virus pandemic. There is an enormous amount of uncertainty about the disease, what the symptoms are, fatality rate, treatment options, things we shouldn't be doing. We're going to try to get away from the headlines and talk about what we need to know - to hopefully give...

In this week’s episode, we discuss bystander guilt, convergence, brain hacks and “how you can sneeze on someone’s brain from anywhere in the world”. How can GPs cope with the myriad worries around treating patients during the current pandemic, both on the frontline and in general practice? How do we recognise and break unhelpful anxious behaviour...

Continuous glucose monitor (CGM) readings are delayed relative to blood glucose, and this delay is usually attributed to the latency of interstitial glucose levels. However, CGM-independent data suggest rapid equilibration of interstitial glucose. This study sought to determine the loci of CGM delays. Electrical current was measured directly from CGM electrodes to define sensor kinetics in the absence of smoothing algorithms. CGMs were implanted in mice, and sensor versus blood glucose responses were measured after an intravenous glucose challenge. Dispersion of a fluorescent glucose analog (2-NBDG) into the CGM microenvironment was observed in vivo using intravital microscopy. Tissue deposited on the sensor and nonimplanted subcutaneous adipose tissue was then collected for histological analysis. The time to half-maximum CGM response in vitro was 35 ± 2 s. In vivo, CGMs took 24 ± 7 min to reach maximum current versus 2 ± 1 min to maximum blood glucose (P = 0.0017). 2-NBDG took 21 ± 7 min to reach maximum fluorescence at the sensor versus 6 ± 6 min in adipose tissue (P = 0.0011). Collagen content was closely correlated with 2-NBDG latency (R = 0.96, P = 0.0004). Diffusion of glucose into the tissue deposited on a CGM is substantially delayed relative to interstitial fluid. A CGM that resists fibrous encapsulation would better approximate real-time deviations in blood glucose.

Amid aging populations and the growth of chronic diseases, the demand for skilled health-care professionals is on the rise in the United States, Canada, and Mexico. This report explores the policy implications, benefits, and challenges of harmonizing nursing qualifications in the region, suggesting that a more collaborative approach could result in greater supply and quality of nurses.

Joseph Tran
Oct 1, 2012; 30:173-178
Practical Pointers

Evan M. Benjamin
Jan 1, 2002; 20:
Practical Pointers

Tadej Battelino
Aug 1, 2019; 42:1593-1603
International Consensus Report

Dorothee Deiss
Dec 1, 2006; 29:2730-2732
BR Emerging Treatments and Technologies

Thomas Danne
Dec 1, 2017; 40:1631-1640
Continuous Glucose Monitoring and Risk of Hypoglycemia

William L Clarke
Sep 1, 1987; 10:622-628
Technical Article

French President Emanuel Macron led a series of events in Europe Friday that commemorate the 75th anniversary of Victory in Europe Day, with a small ceremony in Paris.

David C. Klonoff
Aug 1, 2018; 41:1681-1688
Emerging Technologies: Data Systems and Devices

Kirstine J. Bell
Jun 1, 2015; 38:1008-1015
Type 1 Diabetes at a Crossroads

Richard M. Bergenstal
Nov 1, 2018; 41:2275-2280
Perspectives in Care

Guillermo E. Umpierrez
Aug 1, 2018; 41:1579-1589
Diabetes Care Symposium

Thomas Danne
Dec 1, 2017; 40:1631-1640
Continuous Glucose Monitoring and Risk of Hypoglycemia

Tadej Battelino
Aug 1, 2019; 42:1593-1603
International Consensus Report

OBJECTIVE

To determine if temporal glucose profiles differed between 1) women who were randomized to real-time continuous glucose monitoring (RT-CGM) or self-monitored blood glucose (SMBG), 2) women who used insulin pumps or multiple daily insulin injections (MDIs), and 3) women whose infants were born large for gestational age (LGA) or not, by assessing CGM data obtained from the Continuous Glucose Monitoring in Women With Type 1 Diabetes in Pregnancy Trial (CONCEPTT).

Evan M. Benjamin
Jan 1, 2002; 20:
Practical Pointers

James Chamberlain
Jul 1, 2013; 31:106-109
Feature Articles

Human trafficking.

Paris : G. Steinheil, 1892.