sam Development of SPACE-II for rapid sample exchange at SPring-8 macromolecular crystallography beamlines By scripts.iucr.org Published On :: 2020-01-31 Reducing the sample-exchange time is a crucial issue in maximizing the throughput of macromolecular crystallography (MX) beamlines because the diffraction data collection itself is completed within a minute in the era of pixel-array detectors. To this end, an upgraded sample changer, SPACE-II, has been developed on the basis of the previous model, SPACE (SPring-8 Precise Automatic Cryo-sample Exchanger), at the BL41XU beamline at SPring-8. SPACE-II achieves one sample-exchange step within 16 s, of which its action accounts for only 11 s, because of three features: (i) the implementation of twin arms that enable samples to be exchanged in one cycle of mount-arm action, (ii) the implementation of long-stroke mount arms that allow samples to be exchanged without withdrawal of the detector and (iii) the use of a fast-moving translation and rotation stage for the mount arms. By pre-holding the next sample prior to the sample-exchange sequence, the time was further decreased to 11 s in the case of automatic data collection, of which the action of SPACE-II accounted for 8 s. Moreover, the sample capacity was expanded from four to eight Uni-Pucks. The performance of SPACE-II has been demonstrated in over two years of operation at BL41XU; the average number of samples mounted on the diffractometer in one day was increased from 132 to 185, with an error rate of 0.089%, which counted incidents in which users could not continue with an experiment without recovery work by entering the experimental hutch. On the basis of these results, SPACE-II has been installed at three other MX beamlines at SPring-8 as of July 2019. The fast and highly reliable SPACE-II is now one of the most important pieces of infrastructure for the MX beamlines at SPring-8, providing users with the opportunity to fully make use of limited beamtime with brilliant X-rays. Full Article text
sam Sample deposition onto cryo-EM grids: from sprays to jets and back By scripts.iucr.org Published On :: 2020-03-25 Despite the great strides made in the field of single-particle cryogenic electron microscopy (cryo-EM) in microscope design, direct electron detectors and new processing suites, the area of sample preparation is still far from ideal. Traditionally, sample preparation involves blotting, which has been used to achieve high resolution, particularly for well behaved samples such as apoferritin. However, this approach is flawed since the blotting process can have adverse effects on some proteins and protein complexes, and the long blot time increases exposure to the damaging air–water interface. To overcome these problems, new blotless approaches have been designed for the direct deposition of the sample on the grid. Here, different methods of producing droplets for sample deposition are compared. Using gas dynamic virtual nozzles, small and high-velocity droplets were deposited on cryo-EM grids, which spread sufficiently for high-resolution cryo-EM imaging. For those wishing to pursue a similar approach, an overview is given of the current use of spray technology for cryo-EM grid preparation and areas for enhancement are pointed out. It is further shown how the broad aspects of sprayer design and operation conditions can be utilized to improve grid quality reproducibly. Full Article text
sam The TELL automatic sample changer for macromolecular crystallography By scripts.iucr.org Published On :: 2020-03-31 In this paper, the design and functionalities of the high-throughput TELL sample exchange system for macromolecular crystallography is presented. TELL was developed at the Paul Scherrer Institute with a focus on speed, storage capacity and reliability to serve the three macromolecular crystallography beamlines of the Swiss Light Source, as well as the SwissMX instrument at SwissFEL. Full Article text
sam High-viscosity sample-injection device for serial femtosecond crystallography at atmospheric pressure By scripts.iucr.org Published On :: 2019-10-17 A sample-injection device has been developed at SPring-8 Angstrom Compact Free-Electron Laser (SACLA) for serial femtosecond crystallography (SFX) at atmospheric pressure. Microcrystals embedded in a highly viscous carrier are stably delivered from a capillary nozzle with the aid of a coaxial gas flow and a suction device. The cartridge-type sample reservoir is easily replaceable and facilitates sample reloading or exchange. The reservoir is positioned in a cooling jacket with a temperature-regulated water flow, which is useful to prevent drastic changes in the sample temperature during data collection. This work demonstrates that the injector successfully worked in SFX of the human A2A adenosine receptor complexed with an antagonist, ZM241385, in lipidic cubic phase and for hen egg-white lysozyme microcrystals in a grease carrier. The injection device has also been applied to many kinds of proteins, not only for static structural analyses but also for dynamics studies using pump–probe techniques. Full Article text
sam Successful sample preparation for serial crystallography experiments By scripts.iucr.org Published On :: 2019-11-14 Serial crystallography, at both synchrotron and X-ray free-electron laser light sources, is becoming increasingly popular. However, the tools in the majority of crystallization laboratories are focused on producing large single crystals by vapour diffusion that fit the cryo-cooled paradigm of modern synchrotron crystallography. This paper presents several case studies and some ideas and strategies on how to perform the conversion from a single crystal grown by vapour diffusion to the many thousands of micro-crystals required for modern serial crystallography grown by batch crystallization. These case studies aim to show (i) how vapour diffusion conditions can be converted into batch by optimizing the length of time crystals take to appear; (ii) how an understanding of the crystallization phase diagram can act as a guide when designing batch crystallization protocols; and (iii) an accessible methodology when attempting to scale batch conditions to larger volumes. These methods are needed to minimize the sample preparation gap between standard rotation crystallography and dedicated serial laboratories, ultimately making serial crystallography more accessible to all crystallographers. Full Article text
sam Neutron Larmor diffraction on powder samples By scripts.iucr.org Published On :: 2020-02-01 A hitherto unrecognized resolution effect in neutron Larmor diffraction (LD) is reported, resulting from small-angle neutron scattering (SANS) in the sample. Small distortions of the neutron trajectories by SANS give rise to a blurring of the Bragg angles of the order of a few hundredths of a degree, leading to a degradation of the momentum resolution. This effect is negligible for single crystals but may be significant for polycrystalline or powder samples. A procedure is presented to correct the LD data for the parasitic SANS. The latter is accurately determined by the SESANS technique (spin–echo small-angle neutron scattering), which is readily available on Larmor diffractometers. The analysis technique is demonstrated on LD and SESANS data from α-Fe2O3 powder samples. The resulting d-spacing range agrees with experimental data from high-resolution synchrotron radiation powder diffraction on the same sample. Full Article text
sam AMi: a GUI-based, open-source system for imaging samples in multi-well plates By scripts.iucr.org Published On :: 2019-08-06 Described here are instructions for building and using an inexpensive automated microscope (AMi) that has been specifically designed for viewing and imaging the contents of multi-well plates. The X, Y, Z translation stage is controlled through dedicated software (AMiGUI) that is being made freely available. Movements are controlled by an Arduino-based board running grbl, and the graphical user interface and image acquisition are controlled via a Raspberry Pi microcomputer running Python. Images can be written to the Raspberry Pi or to a remote disk. Plates with multiple sample wells at each row/column position are supported, and a script file for automated z-stack depth-of-field enhancement is written along with the images. The graphical user interface and real-time imaging also make it easy to manually inspect and capture images of individual samples. Full Article text
sam New candidate for “coldest star” is same temperature as a hot cup of coffee By insider.si.edu Published On :: Thu, 24 Mar 2011 14:58:26 +0000 There is a new candidate for coldest known star: a brown dwarf with about the same temperature as a hot cup of coffee. That’s cool enough to begin crossing the blurry line between small cold stars and big hot planets. The post New candidate for “coldest star” is same temperature as a hot cup of coffee appeared first on Smithsonian Insider. Full Article Research News Science & Nature Space astronomy astrophysics Center for Astrophysics | Harvard & Smithsonian Smithsonian Astrophysical Observatory
sam Ability to raft with flotsam and use non-reef habitats helps tropical fish journey to new places, study finds By insider.si.edu Published On :: Tue, 20 Sep 2011 18:44:36 +0000 Depending on where the fish disperse from, the use of ‘stepping stones', flotsam or simply being an adult can help in the journey to find a new home. The post Ability to raft with flotsam and use non-reef habitats helps tropical fish journey to new places, study finds appeared first on Smithsonian Insider. Full Article Animals Marine Science Research News Science & Nature biodiversity conservation conservation biology endangered species fishes Tropical Research Institute
sam Fungal fidelity: some ants have been eating the same meal for 5 million years! By insider.si.edu Published On :: Wed, 30 May 2012 12:49:40 +0000 Scientists have discovered an incredible story of fungal fidelity among certain species of ants. The post Fungal fidelity: some ants have been eating the same meal for 5 million years! appeared first on Smithsonian Insider. Full Article Animals Research News Science & Nature ants Ecuador fungi insects National Museum of Natural History prehistoric South America
sam Research shows same growth rate for farming, non-farming societies By insider.si.edu Published On :: Tue, 22 Dec 2015 13:37:10 +0000 Prehistoric human populations of hunter-gatherers in a region of North America grew at the same rate as farming societies in Europe, according to a new […] The post Research shows same growth rate for farming, non-farming societies appeared first on Smithsonian Insider. Full Article Anthropology Dinosaurs & Fossils Research News Science & Nature Spotlight agriculture astronomy astrophysics Center for Astrophysics | Harvard & Smithsonian
sam How To Email To Multiple Addresses At The Same Time By www.bleepingcomputer.com Published On :: 2006-02-04T01:44:40-05:00 Full Article
sam Samuel P. Langley, Secretary of the Smithsonian, and his development of the ill-fated Aerodrome A By insider.si.edu Published On :: Thu, 21 Feb 2013 14:47:11 +0000 The post Samuel P. Langley, Secretary of the Smithsonian, and his development of the ill-fated Aerodrome A appeared first on Smithsonian Insider. Full Article Video National Air and Space Museum
sam Question About Samsung Muse By www.bleepingcomputer.com Published On :: 2015-06-13T05:00:18-05:00 Full Article
sam My Samsung YP- R0 player can't complete booting process! By www.bleepingcomputer.com Published On :: 2017-06-30T20:40:10-05:00 Full Article
sam A first: New website reveals origin of genetic samples and date collected By insider.si.edu Published On :: Fri, 04 Aug 2017 14:28:48 +0000 For the first time, a new public database will link genetic data with records of where and when the samples it was taken from were […] The post A first: New website reveals origin of genetic samples and date collected appeared first on Smithsonian Insider. Full Article Animals Marine Science Plants Research News Science & Nature National Museum of Natural History
sam Simulink - Incorrect Code Generation: In a model containing blocks from the SoC Blockset and asynchronous sample time, the sorted order might be incorrect By in.mathworks.com Published On :: Fri, 08 May 2020 14:04:15 +0000 Simulink might produce an incorrect sorted order for a model that meets all of the following conditions:The model contains blocks from the SoC BlocksetThe Signal logging option is selected in the model configuration setSignals using asynchronous sample time are configured for loggingAs a result, Simulink might produce incorrect results in Normal, Accelerator, and Rapid Accelerator simulation modes as well as in generated code.This bug exists in the following release(s): R2020a Interested in Upgrading? Full Article
sam Samsung Contacts vs Google Contacts By www.bleepingcomputer.com Published On :: 2020-01-08T12:21:02-05:00 Full Article
sam My old phone without sim card shows same ip address as my active phone By www.bleepingcomputer.com Published On :: 2020-03-20T23:40:30-05:00 Full Article
sam What's Windows 10/Android 5.1 on the same tablet? By www.bleepingcomputer.com Published On :: 2018-07-22T23:10:53-05:00 Full Article
sam Can my Samsung Tab S2 do editing with a keyboard from the SD card? By www.bleepingcomputer.com Published On :: 2018-10-14T04:56:24-05:00 Full Article
sam Samsung Tab S2 (SM-T813) By www.bleepingcomputer.com Published On :: 2019-07-12T08:26:42-05:00 Full Article
sam Samsung Galaxy Tab 10 A help By www.bleepingcomputer.com Published On :: 2019-10-07T23:01:30-05:00 Full Article
sam Detect Samsung Galaxy S5 phone on Win XP? By www.bleepingcomputer.com Published On :: 2020-04-26T15:14:07-05:00 Full Article
sam New Report Recommends Construction of Four New Polar Icebreakers of the Same Design as the Lowest-Cost Strategy for Protecting U.S. Interests in Arctic and Antarctic By feedproxy.google.com Published On :: Tue, 11 Jul 2017 05:00:00 GMT The U.S. lacks icebreaking capability in the Arctic and Antarctic and should build four polar icebreakers with heavy icebreaking capability to help minimize the life-cycle costs of icebreaker acquisition and operations, says a new congressionally mandated letter report from the National Academies of Sciences, Engineering, and Medicine. Full Article
sam To Increase Protection of Miners from Black Lung Disease, A Comprehensive Report on Underground Coal Mine Dust Exposure Says Monitoring and Sampling Should Go Beyond Regulatory Compliance By feedproxy.google.com Published On :: Thu, 28 Jun 2018 05:00:00 GMT Black lung disease cases in coal miners have been increasing since 2000 for uncertain reasons. Full Article
sam New Investments Are Needed to Sustain NASA’s Instrumentation and Facilities for Future Extraterrestrial Sample Analyses, Says New Report By feedproxy.google.com Published On :: Thu, 20 Dec 2018 06:00:00 GMT NASA’s investment in new instruments to analyze extraterrestrial samples is insufficient to provide for replacement of existing instruments, says a new report from the National Academies of Sciences, Engineering, and Medicine. Full Article
sam Samsung, SoFi partner to launch Samsung Pay debit card By feedproxy.google.com Published On :: Fri, 08 May 2020 15:18:00 +0200 Samsung has announced plans of launching a Samsung Pay debit card in the summer of 2020. Full Article
sam Samsung, SoFi partner to launch Samsung Pay debit card By feedproxy.google.com Published On :: Fri, 08 May 2020 15:18:00 +0200 Samsung has announced plans... Full Article
sam Samsung Galaxy S7 Edge Help By www.bleepingcomputer.com Published On :: 2018-06-01T02:00:38-05:00 Full Article
sam VMware, Citrix latch on to Samsung DeX to solve complexity around enterprise mobility and desktop experience By cio.economictimes.indiatimes.com Published On :: 2017-03-31T13:22:02+05:30 Both VMware and Citrix have partnered with Samsung around new Galaxy S8 smartphone through the integration of their enterprise mobility solutions with Samsung DeX platform Full Article
sam Seafloor trawling's ecological impacts revealed by simple sampling system By ec.europa.eu Published On :: Thu, 30 APR 2014 9:23:19 GMT Climate change will substantially increase the severity and length of droughts in Europe by the end of the century, according to new research. The study showed that some European countries could experience a reduction in river flow of up to 80% by the 2080s. Full Article
sam Plastic found in stomachs of over 1 in 6 large pelagic fish sampled in Mediterranean Sea By ec.europa.eu Published On :: Thu, 16 July 2015 9:23:19 GMT Plastic pollution in the ocean is a growing problem. This study, which is the first to investigate the presence of plastic debris in large pelagic fish in the central Mediterranean Sea, found that over 18% of fish had ingested plastics. Full Article
sam Covid-19 fear: Electronics brands Samsung, Apple let offline stores sell online By cio.economictimes.indiatimes.com Published On :: 2020-04-18T12:19:00+05:30 Samsung has created an ecommerce platform in partnership with Benow.in along with mobile phone retailers across the country that will allow them to sell and deliver smartphones. Offline stores are also being used to fulfil orders placed on Samsung’s e-store for television and appliances. Full Article
sam Half of employees watch adult content on the same devices they use for working from home: Kaspersky By cio.economictimes.indiatimes.com Published On :: 2020-05-06T17:22:48+05:30 As social distancing measures become common and workers adjust to their new professional environments, even from their own living rooms, there is potential for private and working life to blur into one. Full Article
sam Seafloor trawling's ecological impacts revealed by simple sampling system By ec.europa.eu Published On :: Thu, 30 APR 2014 9:23:19 GMT Trawling's impacts on marine ecosystems can be assessed using simple metrics which characterise easy-to-obtain samples of fish, new research suggests. The Portuguese study shows that groups of fish become dominated by fewer species as fishing intensity increases, while their total biomass declines. Moreover, in the most fished areas there were other noteworthy changes, such as substantial reductions in the proportion of sharks and rays. Full Article
sam SPONSORED: Never the same day twice working at Celtic Manor By www.watfordobserver.co.uk Published On :: Wed, 26 Apr 2017 11:37:16 +0100 Pull quote: If it wasn’t for the amazing training, I wouldn’t be where I am today Full Article
sam Same-sex marriage may improve public health By www.mnn.com Published On :: Wed, 27 Feb 2013 20:14:01 +0000 Cohabitating doesn't provide the same health benefits for gay couples that marriage does for straights. Full Article Fitness & Well-Being
sam Same-sex marriage: 6 landmark cases that changed U.S. families By www.mnn.com Published On :: Wed, 26 Jun 2013 16:19:56 +0000 The same-sex marriage rulings by the Supreme Court are but the most recent in a long history of cases that reshaped the family structure. Full Article Politics
sam Food pyramids change with time and place, but the goal is the same: Eat well By www.mnn.com Published On :: Wed, 23 Jan 2019 20:24:23 +0000 Colorful diagrams help us understand how much of which foods we should be eating. Full Article Healthy Eating
sam Creationists and conspiracy theorists share the same cognitive error, study finds By www.mnn.com Published On :: Tue, 21 Aug 2018 00:12:46 +0000 Do you believe that "everything happens for a reason"? You might be making this error, too. Full Article Fitness & Well-Being
sam Two wind turbines self-destruct in same week By www.mnn.com Published On :: Tue, 03 Nov 2009 21:45:26 +0000 Turbines in Denmark and Sweden throw off blades — a rare event, but one to consider as alternative energy use grows across globe. Full Article Energy
sam Fukushima radiation leak the same as 76 million bananas By www.mnn.com Published On :: Tue, 13 Aug 2013 15:49:02 +0000 An odd, fruity equation provides perspective on the amount of radioactive water leaking into the Pacific. Full Article Wilderness & Resources
sam Living things unlike anything else on Earth found in random dirt sample By www.mnn.com Published On :: Wed, 21 Nov 2018 23:01:57 +0000 A whole new kingdom will need to be created to classify Hemimastix kukwesjijk, the organisms found in a random dirt sample. Full Article Wilderness & Resources
sam Bin Laden and oil: Some things change, others stay the same By www.mnn.com Published On :: Mon, 02 May 2011 20:09:11 +0000 In the 10 years since Osama bin Laden became a household name, we have fought a war on terror, watched oil prices skyrocket and continued to get oil from the sa Full Article Politics
sam Uncle Sam says use sunscreen By www.mnn.com Published On :: Mon, 23 May 2011 21:59:43 +0000 If a bad burn isn't motivation enough to use care when you're out in the sun, perhaps some government intervention will help. Full Article Fitness & Well-Being
sam What Ebola can teach astronauts about safely handling Martian samples on Earth By www.space.com Published On :: Mon, 03 Nov 2014 15:43:06 +0000 Measures taken in the current Ebola outbreak may hold some clues for how to handle samples brought back to Earth from Mars. Full Article Space
sam Why we don't all see gift-giving the same way By www.mnn.com Published On :: Wed, 09 Dec 2015 20:16:12 +0000 How can men and women get on the same page about gifts? Full Article Arts & Culture
sam Andy Samberg moderates climate insanity By www.mnn.com Published On :: Thu, 15 Jul 2010 00:50:10 +0000 Lexus-sponsored 'debate' between climate denier Phelim McAleer and green girl Simran Sethi yields important lessons for an ailing climate movement... especially Full Article Research & Innovations
sam Sam Sifton's 'Thanksgiving: How to Cook it Well' is $1.99 for Kindle or Nook By www.mnn.com Published On :: Sun, 24 Nov 2013 17:56:33 +0000 Guide to pulling off a traditional Thanksgiving dinner with style from the former New York Times restaurant critic is a bargain. Full Article Recipes