How did you feel? Well, considering I spent almost no time doing anything but working on the business, this is sorta a tough one to answer. Most of the time when I want to unplug I may disappear for an entire weekend to get away from the daily work habits that flow over into the […]

It's every cat's greatest dream to finally catch that red dot. And sometimes they do (meaning we get tired of holding down the laser and pointing). 

During Tuesday night’s Save Your Vote Special Broadcast Event, attorney Gerald Griggs, who serves as the President of the NAACP of Georgia, called for action to counter the increasing threats to voters of color from white supremacist groups. It’s a threat that the Palast Investigations team knows is... READ MORE

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If you're looking to save big on your next outdoor furniture splurge, now's the ultimate time. READ MORE...

The topic of domestic violence is an emotive one conjuring visions of child abuse by parents or carers, or marital violence, in general abuse by men of their wives or partners. According to published police statistics in Scotland for the years of 2012 – 13 male violence of women accounted for 80% of all domestic abuse, and in 2014 over 2,600 children in Scotland were identified as needing protection from abuse. This is particularly concerning since the NSPCC suggests that, for every child who has been identified, there are 8 other children who are at risk but who are ‘under the radar’. These statistics, highlighting the underlying nature of inter-family abuse relationships, i.e. the abuse of less powerful and more vulnerable family members by more powerful adults, undoubtedly account for the majority of the abuse situations within family homes. However this is, sadly, not the whole story. Understanding abuse within a family means recognising the impact of sibling aggression on every family member. It also needs to encompass the growing recognition of child to parent aggression and it is this latter aspect of inter-family relationships with which this article is primarily concerned.

One of the oldest conspiracy and 'black helicopter' frauds is now facing the hand of the law. Alex Jones is reaping what he sowed.

During a portion of his Info Wars program, Alex Jones lamented over how his show is being stripped and sold off after a just verdict against him for his lies about Sandy Hook victims and their families.

A verdict he described as a "Nazi show trial."

Sucks to be you, Alex. And it couldn't happen to a more deserving guy.

JONES: InfoWars, the equipment, InfoWars.com, InfoWarsStore.com, and a whole bunch of other stuff is at a federal bankruptcy auction from the fake judgments and the rigged trials where I was found guilty beforehand and they had literal show trials out of the Soviet Union or Nazi Germany.

Robin, I walked in here during the break after getting a glass of water, saw the auctioneers inside the building, going around surveying from the last time they were here to make sure all the stuff's here. Everything tagged, everything marked, this death.

Good riddance, you motherfrakker.

Rudy Giuliani is trying to avoid the same fate.

We need more of these creeps to face the same punishment.

read more

A one-pan Greek style rice with beef and eggplant in a tasty tomato sauce! Moussaka is a Greek dish typically containing layers of eggplant, a meat sauce, and a bechamel sauce and it takes a bit of time to make it. This dish is inspired by the flavours of moussaka as a quick and easy...

Read On →

The post Greek Style Beef and Eggplant Rice (aka Moussaka Rice) appeared first on Closet Cooking.

I’m at a briefing with a client, along with a board made up of the client’s in-house developers and designers. While discussing an idea with some of the other people in the room, the client stands up and starts hitting the desk with the newspaper.

Me: “Excuse me, but what are you doing?”

Client: “Hush, this works on my dogs at home. It should work on you too.”

He beats the table with the newspaper for a few seconds.

Client: “Now, sit, and stay.”

He continues to beat the table. 

Me: “I’m sorry, this is ridiculous. I’m terminating you as a client.”

As I walked towards the door, the client jumped up from their seat and blocked the door.

Client: “If you want a snack, I’ve got some under my desk, no need to leave the meeting!”

At this point, I shoved him aside, and walked out, followed by him. Whistling, and shouting “come!”

Medical Aid, a South African insurance comparison website, investigated the effects of environmental issues like poor water quality, pollution, sanitation and chemical exposure on the health of citizens in 103 countries around the world. The information is summarized in a report titled, "Climate Health: Where in the World is Climate Change Impacting Health the Most?"[...]

The clonal quaking aspen known as Pando is between 16,000 and 80,000 years old.

The post Famous Paintings Get “Hedgehogged” by a Russian Artist appeared first on English Russia.

The National Science Foundation has awarded a $52.66 million grant to fund a major infrastructure upgrade to the Simons Observatory in the Atacama Desert of Chile. Upgrades are expected to take about five years; the resulting facility will be known as the Advanced Simons Observatory (ASO).

The Oscar-nominated trio behind 'A Single Life' is back with a new dark comedy about climate change and individual responsibility.

RSS Ground is preparing a major update for its blog posters. And we would like to share some thoughts on blogging and its place in today’s life. Just recently “blogging” was extremely popular. Personal and business blogs are a part of our everyday’s life that we can hardly remember the times when it was otherwise. […]

The post Is Blogging Still Important For Life And Business? appeared first on RSSground.com.

We have finally completed the renovation of our tools interfaces. Improved and unified layouts across all of our content generators and posters. And today it’s time to introduce updated WordPress and Blogger posters. These brand new content sharing tools will make a process of automated blog posting work like a charm. WordPress Poster User Guide […]

The post Meet New Automated WordPress and Blogger Posters appeared first on RSSground.com.

We’ve recently tapped into the power of automation with our integrations with IFTTT, Zapier, and Pabbly Connect! Now, you can effortlessly manage your content across the web, extending your reach and impact through automated posting campaigns! Learn more about RSS Ground integration with major automation platforms But hold onto your hats, because we’re just getting […]

The post Personal feeds as new triggers in Zapier, IFTTT & Pabbly Connect appeared first on RSSground.com.

Lately, we’ve noticed an increased number of reports indicating issues with Blogger posting campaigns, where the error message “(403) We’re sorry, but one or more limits for the requested action have been exceeded” is displayed. To make it easier for you, in Blogger Poster we use our own Google Cloud app to make posts to […]

The post Blogger Poster custom apps for volume posting appeared first on RSSground.com.

“Me being 6 feet 7 inches, I’m not your normal height-weight type cyclist. But that motivates me to get better, to be better, to go against these guys.”

At an Arizona tribal school, it's a fierce campaign to pick the top school lunch, as students learn about making their voice count

From the horror of the Holocaust to the struggles that the cousins face in the modern day, the movie is about the overall pains of life, film star Jennifer Grey iterated.

By David Blackmon In the wake of the election of President Donald Trump to serve a second term in office, along with presumptive Republican majorities in both houses of Congress, many are now asking about what the future will hold for the oddly named Inflation Reduction Act. Trump made it repeatedly clear on the campaign […]

The post Biden’s Signature Climate ‘Boondoggle’ Might Be On Chopping Block After Trump Win appeared first on Liberty Unyielding.

BBC F1 correspondent Andrew Benson answers your questions following the Sao Paulo Grand Prix.

Tyrone Mings became the first Englishman to concede a penalty on his Champions League debut in bizarre fashion as Aston Villa lost to Brugge.

1. iPhone SE 4 expected to launch in March 2025, supply chain data suggests  Business Standard
2. iPhone SE4 is likely to launch soon and here are 5 big upgrades we are expecting  India Today
3. Apple iPhone SE 4 to launch next year, likely to become the cheapest iPhone to support Apple Intelligence...  Moneycontrol
4. iPhone SE 4 may spell trouble for iPhone 16 and premium Android phones, here’s why  Hindustan Times
5. iPhone SE 4 Camera Production to Begin Soon Ahead of Anticipated Debut in March 2025: Report  Gadgets 360

If Trump's proposed 60% tariff against China is enacted and the country responds aggressively, it could pressure some of America's largest companies.

Companies have continued to cut jobs in 2024 after a wave of layoffs last year. Meta, Goldman Sachs, Google, and Tesla are among those shedding staff.

There is a reason Joe Rogan became the world's largest podcaster. He doesn't take his audience -- or his guests -- for granted. He wants MEANINGFUL conversations.

The post Joe Rogan Officially Backs A Candidate… Could Be The Biggest Endorsement Yet appeared first on Clash Daily.

There's a reason Hitler holds a special place of disgrace in public memory. He combined the ambition and destruction of Attila The Hun with the personal malice of a serial killer.

The post The REAL Harm Done By Calling Everyone You Don’t Like ‘Hitler’ Is BIGGER Than Lefties Think appeared first on Clash Daily.

Even after her loss on Nov. 5, Vice President Kamala Harris’ election campaign is still hounding donors for money. Harris’ campaign has bombarded supporters with fundraising messages following her election […]

The post Kamala's Campaign Is Still Aggressively Shaking Down Supporters For Cash appeared first on The Western Journal.

Left-wing networks had plenty of bad news for their viewers as former President Donald Trump stormed to victory in the Nov. 5 election. Now, they’re getting plenty of bad news […]

The post Insult to Injury: MSNBC and CNN Suffer Staggering Ratings Plunge Following Trump Victory appeared first on The Western Journal.

Nonphotochemical quenching (NPQ) is a mechanism of regulating light harvesting that protects the photosynthetic apparatus from photodamage by dissipating excess absorbed excitation energy as heat. In higher plants, the major light-harvesting antenna complex (LHCII) of photosystem (PS) II is directly involved in NPQ. The aggregation of LHCII is proposed to be involved in quenching. However, the lack of success in isolating native LHCII aggregates has limited the direct interrogation of this process. The isolation of LHCII in its native state from thylakoid membranes has been problematic because of the use of detergent, which tends to dissociate loosely bound proteins, and the abundance of pigment–protein complexes (e.g. PSI and PSII) embedded in the photosynthetic membrane, which hinders the preparation of aggregated LHCII. Here, we used a novel purification method employing detergent and amphipols to entrap LHCII in its natural states. To enrich the photosynthetic membrane with the major LHCII, we used Arabidopsis thaliana plants lacking the PSII minor antenna complexes (NoM), treated with lincomycin to inhibit the synthesis of PSI and PSII core proteins. Using sucrose density gradients, we succeeded in isolating the trimeric and aggregated forms of LHCII antenna. Violaxanthin- and zeaxanthin-enriched complexes were investigated in dark-adapted, NPQ, and dark recovery states. Zeaxanthin-enriched antenna complexes showed the greatest amount of aggregated LHCII. Notably, the amount of aggregated LHCII decreased upon relaxation of NPQ. Employing this novel preparative method, we obtained a direct evidence for the role of in vivo LHCII aggregation in NPQ.

India Budget 2018: Who are the Biggest Winners and Losers? Expert comment sysadmin 2 February 2018

Any claims to boost farmers’ prospects will inevitably be seen as political, coming ahead of a series of key elections. But this budget recognises India’s most pressing concerns - and seeks to address them.

In 2016 India’s prime minister, Narendra Modi, pledged to double farmers’ incomes by 2022. While the pledge may have been aspirational, reliant on good weather as much as government policy, the 2018/19 budget demonstrates the depth of the government’s commitment to the agriculture sector.

There’s clearly a political edge to any moves to bolster agriculture: any solace to rural India will be described as political opportunism until most people in India are not engaged directly or indirectly in the agricultural sector.

And with a number of state elections coming up this year and a general election next – suggestions that it will be brought forward have increased in volume since the budget – a politically-astute budget is unsurprising. At the same time, there is a real sense of crisis in Indian agriculture which the budget is attempting to ameliorate.

Significant hike in infrastructure spending

The budget – which has Keynesian undertones – announces both direct and indirect support for rural India; it increases significantly the price at which the government purchases agricultural produce, and offers a range of further support through initiatives in areas such as irrigation. Furthermore, there is a significant hike in spending on infrastructure – roads, railways, ports and waterways – with a major allocation for rural infrastructure.

While government spending on infrastructure had been growing, the 2018/19 budget appears to accept that innovative funding models to fund rural projects are unlikely to work – instead, rural highways will have to be funded from overall taxation, as is generally the norm.

The other bold initiative focuses on healthcare. One hundred million poor families (so around half a billion Indians in total) will receive up to Rs500,000 for medical care under a health insurance programme. Given the correlation between ill health and poverty, this should have implications for the economy as well as healthcare itself. At the moment poverty effectively excludes many Indians from private healthcare.

Education too received a boost, with several proposals including teacher training. There have long been concerns over the quality of Indian education. In particular, even where schools exist physically, teachers themselves are absent.

As is often the case with India, the question will be in the implementation. While there are bound to be hiccups, the current Indian government does appear to realise the need for on-the-ground, visible change.

The recent state election in Gujarat demonstrated that while the ruling Bharatiya Janata Party (BJP) is clearly favourite to win another term, it is not invulnerable. If the changes do not reach the “last mile”, then they will accrue neither the economic nor the political benefits.

The downsides? Increased taxes will raise inflation, potentially exacerbated by sustained government borrowing; a 20% rise in customs duty will affect imports of mobile phones (though benefit domestic producers and encourage other foreign firms to set up manufacturing plants in India) and some of the revenue projections appear optimistic.

On balance though, this is a budget that recognises the most pressing challenges which India faces, and attempts to provide solutions to them.

This article was originally published in The Independent.

Philip L. Ross
Dec 1, 2004; 3:1154-1169
Research

Erythromycin-resistance methyltransferases are SAM dependent Rossmann fold methyltransferases that convert A2058 of 23S rRNA to m6 2A2058. This modification sterically blocks binding of several classes of antibiotics to 23S rRNA, resulting in a multidrug-resistant phenotype in bacteria expressing the enzyme. ErmC is an erythromycin resistance methyltransferase found in many Gram-positive pathogens, whereas ErmE is found in the soil bacterium that biosynthesizes erythromycin. Whether ErmC and ErmE, which possess only 24% sequence identity, use similar structural elements for rRNA substrate recognition and positioning is not known. To investigate this question, we used structural data from related proteins to guide site-saturation mutagenesis of key residues and characterized selected variants by antibiotic susceptibility testing, single turnover kinetics, and RNA affinity–binding assays. We demonstrate that residues in α4, α5, and the α5-α6 linker are essential for methyltransferase function, including an aromatic residue on α4 that likely forms stacking interactions with the substrate adenosine and basic residues in α5 and the α5-α6 linker that likely mediate conformational rearrangements in the protein and cognate rRNA upon interaction. The functional studies led us to a new structural model for the ErmC or ErmE-rRNA complex.

Frederic Heihoff
Proc. Amer. Math. Soc. 152 (), 5229-5247.
Abstract, references and article information

In 2020, when Anthony Parker, known to his fans as DJ Specs, decided to trade his textbooks for turntables, he didn't just pivot his career -- he pivoted his entire life. At just 25 years old, this Kingston-born DJ has already carved out a space...

Myelination plays an important role in cognitive development and in demyelinating diseases like multiple sclerosis (MS), where failure of remyelination promotes permanent neuro-axonal damage. Modification of cell surface receptors with branched N-glycans coordinates cell growth and differentiation by controlling glycoprotein clustering, signaling, and endocytosis. GlcNAc is a rate-limiting metabolite for N-glycan branching. Here we report that GlcNAc and N-glycan branching trigger oligodendrogenesis from precursor cells by inhibiting platelet-derived growth factor receptor-α cell endocytosis. Supplying oral GlcNAc to lactating mice drives primary myelination in newborn pups via secretion in breast milk, whereas genetically blocking N-glycan branching markedly inhibits primary myelination. In adult mice with toxin (cuprizone)-induced demyelination, oral GlcNAc prevents neuro-axonal damage by driving myelin repair. In MS patients, endogenous serum GlcNAc levels inversely correlated with imaging measures of demyelination and microstructural damage. Our data identify N-glycan branching and GlcNAc as critical regulators of primary myelination and myelin repair and suggest that oral GlcNAc may be neuroprotective in demyelinating diseases like MS.

VAR2CSA is the placental-malaria–specific member of the antigenically variant Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family. It is expressed on the surface of Plasmodium falciparum-infected host red blood cells and binds to specific chondroitin-4-sulfate chains of the placental proteoglycan receptor. The functional ∼310 kDa ectodomain of VAR2CSA is a multidomain protein that requires a minimum 12-mer chondroitin-4-sulfate molecule for specific, high affinity receptor binding. However, it is not known how the individual domains are organized and interact to create the receptor-binding surface, limiting efforts to exploit its potential as an effective vaccine or drug target. Using small angle X-ray scattering and single particle reconstruction from negative-stained electron micrographs of the ectodomain and multidomain constructs, we have determined the structural architecture of VAR2CSA. The relative locations of the domains creates two distinct pores that can each accommodate the 12-mer of chondroitin-4-sulfate, suggesting a model for receptor binding. This model has important implications for understanding cytoadherence of infected red blood cells and potentially provides a starting point for developing novel strategies to prevent and/or treat placental malaria.

The recent structural elucidation of ex vivo Drosophila Orb2 fibrils revealed a novel amyloid formed by interdigitated Gln and His residue side chains belonging to the prion-like domain. However, atomic-level details on the conformational transitions associated with memory consolidation remain unknown. Here, we have characterized the nascent conformation and dynamics of the prion-like domain (PLD) of Orb2A using a nonconventional liquid-state NMR spectroscopy strategy based on 13C detection to afford an essentially complete set of 13Cα, 13Cβ, 1Hα, and backbone 13CO and 15N assignments. At pH 4, where His residues are protonated, the PLD is disordered and flexible, except for a partially populated α-helix spanning residues 55–60, and binds RNA oligos, but not divalent cations. At pH 7, in contrast, His residues are predominantly neutral, and the Q/H segments adopt minor populations of helical structure, show decreased mobility and start to self-associate. At pH 7, the His residues do not bind RNA or Ca2+, but do bind Zn2+, which promotes further association. These findings represent a remarkable case of structural plasticity, based on which an updated model for Orb2A functional amyloidogenesis is suggested.

Yi Liu
Dec 1, 2020; 19:1968-1985
Research

Adult progenitor cell populations typically exist in a quiescent state within a controlled niche environment. However, various stresses or forms of damage can disrupt this state, which often leads to dysfunction and aging. We built a glucocorticoid (GC)-induced liver damage model of mice, found that GC stress induced liver damage, leading to consequences for progenitor cells expansion. However, the mechanisms by which niche factors cause progenitor cells proliferation are largely unknown. We demonstrate that, within the liver progenitor cells niche, Galectin-3 (Gal-3) is responsible for driving a subset of progenitor cells to break quiescence. We show that GC stress causes aging of the niche, which induces the up-regulation of Gal-3. The increased Gal-3 population increasingly interacts with the progenitor cell marker CD133, which triggers focal adhesion kinase (FAK)/AMP-activated kinase (AMPK) signaling. This results in the loss of quiescence and leads to the eventual stemness exhaustion of progenitor cells. Conversely, blocking Gal-3 with the inhibitor TD139 prevents the loss of stemness and improves liver function. These experiments identify a stress-dependent change in progenitor cell niche that directly influence liver progenitor cell quiescence and function.

Protein quality control is maintained by a number of integrated cellular pathways that monitor the folding and functionality of the cellular proteome. Defects in these pathways lead to the accumulation of misfolded or faulty proteins that may become insoluble and aggregate over time. Protein aggregates significantly contribute to the development of a number of human diseases such as amyotrophic lateral sclerosis, Huntington's disease, and Alzheimer's disease. In vitro, imaging-based, cellular studies have defined key biomolecular components that recognize and clear aggregates; however, no unifying method is available to quantify cellular aggregates, limiting our ability to reproducibly and accurately quantify these structures. Here we describe an ImageJ macro called AggreCount to identify and measure protein aggregates in cells. AggreCount is designed to be intuitive, easy to use, and customizable for different types of aggregates observed in cells. Minimal experience in coding is required to utilize the script. Based on a user-defined image, AggreCount will report a number of metrics: (i) total number of cellular aggregates, (ii) percentage of cells with aggregates, (iii) aggregates per cell, (iv) area of aggregates, and (v) localization of aggregates (cytosol, perinuclear, or nuclear). A data table of aggregate information on a per cell basis, as well as a summary table, is provided for further data analysis. We demonstrate the versatility of AggreCount by analyzing a number of different cellular aggregates including aggresomes, stress granules, and inclusion bodies caused by huntingtin polyglutamine expansion.