List of definitions for interchange circuits between data terminal equipment (DTE) and data circuit-terminating equipment (DCE)

El británico, que ganó seis medallas de oro en los Juegos de 2004, 2008 y 2012, afirma que pese a su enfermedad "se siente afortunado" Leer

Advantech, provider of industrial computing platforms, has launched its new DLT-V73 Series, the next generation rugged vehicle mount terminal (VMT), aimed at industrial applications such as warehouse and port management, heavy-duty machine operations, and manufacturing.

Airport Synergy Group is hiring in Los Angeles CATerminal Manager positions available. Visit us to learn more about Airport Synergy Group and see our job postings on www.avjobs.com Please reference Avjobs when applying. Tell your friends about Airport Synergy Group.

[Updated with more photos] The new airport terminal is scheduled to officially open for operations tomorrow [Dec 9] and the photos below show a small advance look at the new facility. A spokesperson previously said, “The new, purpose-built Airport Terminal will officially open for operations on December 9, 2020. The 288,000 square foot facility will […]

Jesper V. Olsen
Jun 1, 2004; 3:608-614
Technology

Bacterial low-copy-number plasmids require partition (par) systems to ensure their stable inheritance by daughter cells. In general, these systems consist of three components: a centromeric DNA sequence, a centromere-binding protein and a nucleotide hydrolase that polymerizes and functions as a motor. Type III systems, however, segregate plasmids using three proteins: the FtsZ/tubulin-like GTPase TubZ, the centromere-binding protein TubR and the MerR-like transcriptional regulator TubY. Although the TubZ filament is sufficient to transport the TubR-centromere complex in vitro, TubY is still necessary for the stable maintenance of the plasmid. TubY contains an N-terminal DNA-binding helix-turn-helix motif and a C-terminal coiled-coil followed by a cluster of lysine residues. This study determined the crystal structure of the C-terminal domain of TubY from the Bacillus cereus pXO1-like plasmid and showed that it forms a tetrameric parallel four-helix bundle that differs from the typical MerR family proteins with a dimeric anti-parallel coiled-coil. Biochemical analyses revealed that the C-terminal tail with the conserved lysine cluster helps TubY to stably associate with the TubR-centromere complex as well as to nonspecifically bind DNA. Furthermore, this C-terminal tail forms an amphipathic helix in the presence of lipids but must oligomerize to localize the protein to the membrane in vivo. Taken together, these data suggest that TubY is a component of the nucleoprotein complex within the partitioning machinery, and that lipid membranes act as mediators of type III systems.

The HIV-1 protein Gag assembles at the plasma membrane and drives virion budding, assisted by the cellular endosomal complex required for transport (ESCRT) proteins. Two ESCRT proteins, TSG101 and ALIX, bind to the Gag C-terminal p6 peptide. TSG101 binding is important for efficient HIV-1 release, but how ESCRTs contribute to the budding process and how their activity is coordinated with Gag assembly is poorly understood. Yeast, allowing genetic manipulation that is not easily available in human cells, has been used to characterize the cellular ESCRT function. Previous work reported Gag budding from yeast spheroplasts, but Gag release was ESCRT-independent. We developed a yeast model for ESCRT-dependent Gag release. We combined yeast genetics and Gag mutational analysis with Gag-ESCRT binding studies and the characterization of Gag-plasma membrane binding and Gag release. With our system, we identified a previously unknown interaction between ESCRT proteins and the Gag N-terminal protein region. Mutations in the Gag-plasma membrane–binding matrix domain that reduced Gag-ESCRT binding increased Gag-plasma membrane binding and Gag release. ESCRT knockout mutants showed that the release enhancement was an ESCRT-dependent effect. Similarly, matrix mutation enhanced Gag release from human HEK293 cells. Release enhancement partly depended on ALIX binding to p6, although binding site mutation did not impair WT Gag release. Accordingly, the relative affinity for matrix compared with p6 in GST-pulldown experiments was higher for ALIX than for TSG101. We suggest that a transient matrix-ESCRT interaction is replaced when Gag binds to the plasma membrane. This step may activate ESCRT proteins and thereby coordinate ESCRT function with virion assembly.

Synaptic transmission leading to release of neurotransmitters in the nervous system is a fast and highly dynamic process. Previously, protein interaction and phosphorylation have been thought to be the main regulators of synaptic transmission. Here we show that sialylation of N-linked glycosylation is a novel potential modulator of neurotransmitter release mechanisms by investigating depolarization-dependent changes of formerly sialylated N-linked glycopeptides. We suggest that negatively charged sialic acids can be modulated, similarly to phosphorylation, by the action of sialyltransferases and sialidases thereby changing local structure and function of membrane glycoproteins. We characterized site-specific alteration in sialylation on N-linked glycoproteins in isolated rat nerve terminals after brief depolarization using quantitative sialiomics. We identified 1965 formerly sialylated N-linked glycosites in synaptic proteins and found that the abundances of 430 glycosites changed after 5 s depolarization. We observed changes on essential synaptic proteins such as synaptic vesicle proteins, ion channels and transporters, neurotransmitter receptors and cell adhesion molecules. This study is to our knowledge the first to describe ultra-fast site-specific modulation of the sialiome after brief stimulation of a biological system.

We developed a simple and rapid method to enrich protein N-terminal peptides, in which the protease TrypN is first employed to generate protein N-terminal peptides without Lys or Arg and internal peptides with two positive charges at their N-termini, and then the N-terminal peptides with or without N-acetylation are separated from the internal peptides by strong cation exchange chromatography according to a retention model based on the charge/orientation of peptides. This approach was applied to 20 μg of human HEK293T cell lysate proteins to profile the N-terminal proteome. On average, 1,550 acetylated and 200 unmodified protein N-terminal peptides were successfully identified in a single LC/MS/MS run with less than 3% contamination with internal peptides, even when we accepted only canonical protein N-termini registered in the Swiss-Prot database. Since this method involves only two steps, protein digestion and chromatographic separation, without the need for tedious chemical reactions, it should be useful for comprehensive profiling of protein N-termini, including proteoforms with neo-N-termini.

Is there a skill command for "Assign Layout Instance terminals", this form appears when i click on define device correspondence and Bind the devices.

Also,

Problem Statement : i have a schematic with a couple of transistor symbols and and i alos have a corresponding layout view with respective layout transistors but they all are inside a pCell(created by me) i.e layout transistor called inside a custom Pcell. Now i have multiple symbols in schematic view and a single instance(pCell) in layout view. 
Is there a way how i can bind these schematic symbols with layout symbols inside the pCell(custom)? Even if i have to use cph commands i'm fine with it. need help here.

The idea here is to establish XL connectivity between the schematic symbols and corresponding layout transistors(inside the pCell).

Thanks,

Shankar

IC 23.1-64b.ISR8.40

Hi all, I'm trying to run an stb analysis in a dspf extracted view via Probe terminal. The instance exist in the dspf and I already prepended the X that is placed in the dspf extraction.

Spectre complains with the following error:

Error found by spectre during STB analysis `stb'.
    ERROR (SPECTRE-16408): The probe parameter must be specified to perform stability analysis.

Analysis `stb' was terminated prematurely due to an error.

What is missing here?

Title: Doctors' Religious Beliefs Can Color Their Care of Terminally Ill
Category: Health News
Created: 8/26/2010 2:10:00 PM
Last Editorial Review: 8/27/2010 12:00:00 AM

Nik is worried assisted dying could lead to coercion - but Elise, who has cancer, wants the choice.

MIAL is the only Indian airport offering such a service, features nine trained dogs including a Golden Retriever, a Maltese, a rescued Husky, Shih Tzu, Lhasa Apso, and Labrador

While the issue with the of TOS has been addressedsorted out, the trailers are still facing delays in the scanning centre

Positioned strategically between Tuna Tekra and the entry point of Kandla, the proposed site could help vessels avoid an 11-kilometer trek through the creek, providing cost advantages

India, UK to legislate on allowing patients this choice

Chief Minister suggests changes in the design of the expansion works to reflect Andhra Pradesh’s culture and heritage

Prime Minister Narendra Modi will on Friday inaugurate the Terminal 2 of the Kempegowda International Airport in Bengaluru that has been built at a cost of around Rs 5,000 crore, according to officials.

The terminal, set to be the company’s largest mini-LNG facility to date, will provide shore power to cruise ships at the Nassau Cruise Port

State-of-the-art electron microscopy reveals the large-scale organization of the proteins that regulate neurotransmitter release

This spectacular image – which took the best part of a year to create – shows the fine structure of a nerve terminal at high resolution, revealing, for the very first time, an intricate network of fine filaments that controls the movements of synaptic vesicles.

The brain is soft and wet, with the consistency of a lump of jelly. Yet, it is the most complex and highly organized structure that we know of, containing hundreds of billions of neurons and glial cells, and something on the order of one quadrillion synaptic connections, all of which are arranged in a very specific manner.

Related: 3D model of a nerve terminal in atomic detail | Mo Costandi

Related: Blowing up the brain to reveal its finer details

The Q4FY17 PAT was Rs 150 crore (47% y-o-y), 9% below our estimate. PPOP was 2% below estimate driven by lower NII, offset partially by lower costs.

This registry code allows any terminal client access to a Terminal Server. It bypasses the Microsoft "Terminal Server License" and allows the client to create a session on the server without a CAL (Client Access License) or MS Open License. It works on WinNT, Win2000, Win2003 server and Win2008 server.

A huge fire has broken out near the Qantas Terminal at Sydney airport.

Olfactomedins are a family of modular proteins found in multicellular organisms that all contain five-bladed β-propeller olfactomedin (OLF) domains. In support of differential functions for the OLF propeller, the available crystal structures reveal that only some OLF domains harbor an internal calcium-binding site with ligands derived from a triad of residues. For the myocilin OLF domain (myoc-OLF), ablation of the ion-binding site (triad Asp, Asn, Asp) by altering the coordinating residues affects the stability and overall structure, in one case leading to misfolding and glaucoma. Bioinformatics analysis reveals a variety of triads with possible ion-binding characteristics lurking in OLF domains in invertebrate chordates such as Arthropoda (Asp–Glu–Ser), Nematoda (Asp–Asp–His) and Echinodermata (Asp–Glu–Lys). To test ion binding and to extend the observed connection between ion binding and distal structural rearrangements, consensus triads from these phyla were installed in the myoc-OLF. All three protein variants exhibit wild-type-like or better stability, but their calcium-binding properties differ, concomitant with new structural deviations from wild-type myoc-OLF. Taken together, the results indicate that calcium binding is not intrinsically destabilizing to myoc-OLF or required to observe a well ordered side helix, and that ion binding is a differential feature that may underlie the largely elusive biological function of OLF propellers.

The biological processes related to protein homeostasis in Mycobacterium tuberculosis, the etiologic agent of tuberculosis, have recently been established as critical pathways for therapeutic intervention. Proteins of particular interest are ClpC1 and the ClpC1–ClpP1–ClpP2 proteasome complex. The structure of the potent antituberculosis macrocyclic depsipeptide ecumicin complexed with the N-terminal domain of ClpC1 (ClpC1-NTD) is presented here. Crystals of the ClpC1-NTD–ecumicin complex were monoclinic (unit-cell parameters a = 80.0, b = 130.0, c = 112.0 Å, β = 90.07°; space group P21; 12 complexes per asymmetric unit) and diffracted to 2.5 Å resolution. The structure was solved by molecular replacement using the self-rotation function to resolve space-group ambiguities. The new structure of the ecumicin complex showed a unique 1:2 (target:ligand) stoichiometry exploiting the intramolecular dyad in the α-helical fold of the target N-terminal domain. The structure of the ecumicin complex unveiled extensive interactions in the uniquely extended N-terminus, a critical binding site for the known cyclopeptide complexes. This structure, in comparison with the previously reported rufomycin I complex, revealed unique features that could be relevant for understanding the mechanism of action of these potential antituberculosis drug leads. Comparison of the ecumicin complex and the ClpC1-NTD-L92S/L96P double-mutant structure with the available structures of rufomycin I and cyclomarin A complexes revealed a range of conformational changes available to this small N-terminal helical domain and the minor helical alterations involved in the antibiotic-resistance mechanism. The different modes of binding and structural alterations could be related to distinct modes of action.

...

A study of families in Singapore with terminally ill children found that parents tend to defer discussing their psychological pain with their spouses to protect them from emotional distress. The study, conducted by psychologists at NTU through interviews, revealed the parents’ preference to support each other in pragmatic and solution-oriented ways such as discussing treatment options, arranging care plans and sharing caregiving responsibilities....

150 years after outdoor markets fell out of favor with the public at the location, an outdoor farmers market is resurrected at the Philadelphia landmark.

New $48 million installation will provide land and air services to animals of all sizes traveling through JFK airport.

The ARK at JFK will boast luxurious accommodations for all species — but not people.

Product Information

Quality color display with LED backlighting. Complete HMI family. Comprehensive features.(NB Series)

Product Information

Many Variations in Connectors and Number of Poles(XW2R (General-purpose devices))