REVIEW – When you live in a somewhat secluded wooded area like I do, you want to be able to monitor your property when you’re home and away from home. I’ve had multiple security cameras around my house for years, but only in the past year have I “graduated” to PoE cameras, and I love […]

Candida albicans and Aspergillus fumigatus are dangerous fungal pathogens with high morbidity and mortality, particularly in immunocompromised patients. Innate immune-mediated programmed cell death (pyroptosis, apoptosis, necroptosis) is an integral part of host defense against pathogens. Inflammasomes, which are canonically formed upstream of pyroptosis, have been characterized as key mediators of fungal sensing and drivers of proinflammatory responses. However, the specific cell death pathways and key upstream sensors activated in the context of Candida and Aspergillus infections are unknown. Here, we report that C. albicans and A. fumigatus infection induced inflammatory programmed cell death in the form of pyroptosis, apoptosis, and necroptosis (PANoptosis). Further, we identified the innate immune sensor Z-DNA binding protein 1 (ZBP1) as the apical sensor of fungal infection responsible for activating the inflammasome/pyroptosis, apoptosis, and necroptosis. The Zα2 domain of ZBP1 was required to promote this inflammasome activation and PANoptosis. Overall, our results demonstrate that C. albicans and A. fumigatus induce PANoptosis and that ZBP1 plays a vital role in inflammasome activation and PANoptosis in response to fungal pathogens.

Visual Abstract

Weixian Deng
Dec 22, 2020; 0:RA120.002411v1-mcp.RA120.002411
Research

Deep proteome coverage in bottom-up proteomics requires peptide-level fractionation to simplify the complex peptide mixture before analysis by tandem mass spectrometry. By decreasing the number of co-eluting precursor peptide ions, fractionation effectively reduces the complexity of the sample leading to higher sample coverage and reduced bias towards high abundance precursors that are preferentially identified in data-dependent acquisition strategies. To achieve this goal, we report a bead-based off-line peptide fractionation method termed CIF or Carboxylate modified magnetic bead-based isopropanol gradient peptide fractionation. CIF is an extension of the SP3 (single-pot solid-phase-enhanced sample preparation) strategy and provides an effective but complementary approach to other commonly used fractionation methods including strong cation exchange (SCX) and reversed phase (RP)-based chromatography. We demonstrate that CIF is an effective offline separation strategy capable of increasing the depth of peptide analyte coverage both when used alone or as a second dimension of peptide fractionation in conjunction with high pH RP. These features make it ideally suited for a wide range of proteomic applications including the affinity purification of low abundance bait proteins.

Olympic athlete Jackie Joyner-Kersee delivered a motivational keynote address DOVER, Del. (June 6, 2024) – The Delaware Division of Public Health’s (DPH) Physical Activity, Nutrition, and Obesity Prevention (PANO) Program hosted its second annual Advancing Healthy Lifestyles Conference today. At Delaware State University’s (DSU) Martin Luther King Jr. Conference Center, approximately 300 participants came together […]

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Praised as the most beautiful countryside in China, the unique view of shaiqiu can only be found in Huangling village where baskets of colorful harvest bask in the sunshine.

Edward James Olmos

With African governments finding it more comfortable to deal with China, the recent FOCAC conference needs objective scrutiny; there are takeaways for India

Les dépenses de santé en France représentent un peu plus de 11 % du PIB, une des proportions les plus élevées des pays de l’OCDE, et devraient s’élever jusqu’à 13 % du PIB en 2030. Ces dépenses ont contribué à de bons résultats de santé, avec l’espérance de vie supérieure de deux ans à la moyenne de l’OCDE. Encore un adulte sur quatre fume tous les jours et la consommation d’alcool reste environ 30 % supérieure à la moyenne de l’OCDE.

The asymmetric unit of the title inorganic–organic salt, poly[di(μ2-2-hy­droxy­propano­ato)cadmium], [Cd(C3H5O3)2]n or [Cd(Hlac)2]n (H2lac = 2-hy­droxy­propanoic acid), comprises of a cadmium cation and two 2-hy­droxy­propano­ate anions. The cadmium cation exhibits a distorted penta­gonal–bipyramidal coordination environment defined by the hy­droxy and carbonyl O atoms of the 2-hy­droxy­propano­ate anions. The coordination mode leads to the formation of layers extending parallel to (010). O—H⋯O hydrogen bonding between the hy­droxy and carbonyl groups stabilizes the structure packing.

In the title mol­ecule, C13H16N4O3, the mean planes of the phenyl and triazole rings are nearly perpendicular to one another as a result of the intra­molecular C—H⋯O and C—H⋯π(ring) inter­actions. In the crystal, layers parallel to (101) are generated by O—H⋯N, N—H⋯O and N—H⋯N hydrogen bonds. The layers are connected by inversion-related pairs of C—H⋯O hydrogen bonds. The experimental mol­ecular structure is close to the gas-phase geometry-optimized structure calculated by DFT methods. Hirshfeld surface analysis indicates that the most important inter­action involving hydrogen in the title compound is the H⋯H contact. The contribution of the H⋯O, H⋯N, and H⋯H contacts are 13.6, 16.1, and 54.6%, respectively.

Aline A. Alves, Heloisa B. Gabriel, Maria J. R. Bezerra, Wanderley de Souza, Sue Vaughan, Narcisa L. Cunha-e-Silva, and Jack D. Sunter

Eukaryotic flagella are complex microtubule based organelles and in many organisms there are extra-axonemal structures present, including the outer dense fibres of mammalian sperm and the paraflagellar rod (PFR) of trypanosomes. Flagellum assembly is a complex process occurring across three main compartments, the cytoplasm, the transition fibre-transition zone, and the flagellum. It begins with translation of protein components, followed by their sorting and trafficking into the flagellum, transport to the assembly site and then incorporation. Flagella are formed from over 500 proteins; the principles governing axonemal component assembly are relatively clear. However, the coordination and sites of extra-axonemal structure assembly processes are less clear.

We have discovered two cytoplasmic proteins in T. brucei that are required for PFR formation, PFR assembly factors 1 and 2. Deletion of either PFR-AF1 or PFR-AF2 dramatically disrupted PFR formation and caused a reduction in the amount of major PFR proteins. The presence of cytoplasmic factors required for PFR formation aligns with the concept of processes occurring across multiple compartments to facilitate axoneme assembly and this is likely a common theme for extra-axonemal structure assembly.

Aline A. Alves
Apr 15, 2020; 0:jcs.242271v1-jcs.242271
Articles

"Vertical Churches" give viewers a head-turning look at some of the most beautiful architecture in the world.

As kayak fishing continues to grow, Pompano Beach is the perfect fishing hub. The warm water, large accessible beaches and year-round fishing opportunities make Pompano Beach the best place to kayak fish.

Saint Coleman's Men's Club event "Walk on Water Fishing Tournament" partners with Alsco to benefit the Special Olympics of Broward County

Instagram: www.instagram.com/aesrth/

Prizes: Amateur Awards 1st Place – $1500 cash Runner up – $500 cash Categories: VR/360 Awards Nature / Landscapes Built Environment / Architecture Image requirements: All [...]

The post 2020 Epson Pano Awards appeared first on Photocompete.

A novel substituted N-acetyl-L-cysteine (NAC) derivative and methods of using this compound for the treatment of diseases and/or conditions, including but not limited to diseases and/or conditions of, or involving, the Central Nervous System (CNS), including schizophrenia adrenoleukodystrophy, mitochondrial diseases (e.g. Leigh syndrome, Alpers' disease, and MELAS), Huntington's disease, trichotillomania, HIV-associated neurocognitive disorder, hypoxic-ischemic encephalopathy, drug craving, and drug addiction.

The present invention discloses a method of inhibiting an ethylene response in a plant, comprising step of applying to at least one portion of the plant an effective ethylene response-inhibiting amount of a H1-cyclopropene-1-propanoic acid salt (CPAS). A method of prolonging the life of a cut flower, comprising applying to the cut flower an effective life-prolonging amount of CPAS and a method for the production a CPAS, comprising steps of (i) preparing 4-bromo-4-pentenoic acid or derivatives thereof; (ii) producing 1-cyclopropene-1-propanoic acid; and (iii), converting this acid into its water soluble salt, especially its sodium salt are presented. Additionally, a new family of water soluble CPAS inhibitors for ethylene response in a plant is disclosed.

The invention relates to an X-ray device comprising an x-ray sensitive camera for creating tomograms, especially panoramic tomograms. Means for creating 3D shots of a partial volume of the mandibular arch are also provided, said 3D shots being created especially by a second image receiver for creating a 2D shot and means for taking a plurality of 2D shots from different directions and creating a 3D shot therefrom, preferably according to conebeam technology with the associated reconstruction algorithms. The x-ray sensitive camera comprises a first x-ray sensitive image receiver for creating a tomogram, and a second x-ray sensitive image receiver for creating plane shots.

In this episode of “This Song” Elizabeth McQueen sits down with the members of Moving Panoramas. Leslie Sisson, Karen Skloss and Rozie Castoe all talk about songs that changed them and contributed to the sound of Moving Panoramas. You can here the Moving Panorama’s My KUTX DJ set here.   Subscribe via iTunes or Stitcher to get the new […]

Cara Tillman, vocalist and instrumentalist from Moving Panoramas, describes the joy that Elton John's "Burn Down the Mission" brings her, followed by guitarist Rosie Castoe exploring her life long love of Peter Gabriel's "Steam." And lead singer and songwriter Leslie Sisson explains why "Some Song" by Elliot Smith made her feel less alone.

Mount Panorama has made the most experienced racing driver and their team shudder, but it hasn't stopped a group of TAFE students taking on the mountain.

(California Court of Appeal) - In an arbitration action, arising from a dispute between plaintiff electricity producer and defendant utility over which party bears the cost of complying with laws to reduce greenhouse gas emissions under the Global Warming Solutions Act of 2006, the trial court's vacating of the arbitration award to PG&E is reversed where the question of contact interpretation was ripe for arbitration and plaintiff failed to show sufficient cause for postponing the arbitration process.

IBM y Red Hat, líder mundial de software de nube de código abierto, anunciaron un acuerdo en el que IBM adquirirá todas las acciones comunes emitidas y en circulación de Red Hat por USD$190.00 por acción en efectivo, lo que representa un valor total para la empresa de aproximadamente USD$34 mil millones.

Perched atop a hill in Aosta Valley’s highest municipality in northwest Italy is the newly completed House in Chamois, a modern, prefabricated home by Torino-based design and build firm Leap Factory. As with all “Leap Houses,” the home’s entire design and construction process was managed by the Leap Factory team and was constructed with a modular system built of natural, recyclable materials to allow for maximum flexibility. All of the components provided by Leap Factory for the House in Chamois were also designed and produced in Italy. [...]

Experts on this Spanish language webinar examine the operation of today’s interior immigration enforcement system and how state and local governments, civil society, and consulates are responding.    

A Spanish language webinar examining the operation of today’s interior enforcement system and how state and local governments, civil society, and consulates are responding.    

London (24, Cornhill) : Published by F.G. Harding ; [London] (147, Strand) : & J. McCormick, October 1831 ([London] : Printed by Lahee)

Rick talks with the CEO of Panorama Education, an ed-tech company whose college- and career-readiness tools are currently used each year in 11,500 schools.

Tympanometry provides a measure of middle ear function. There has been no description of the relationship between measurements of middle ear function in the absence of gross anatomic defects and clinical outcome among children with acute nontraumatic coma.

This study reveals an unexpected association between abnormal middle ear function and death in childhood acute coma. These findings call for more investigations on the relationship between middle and inner ear anatomy and function and intracranial dynamics and clinical outcomes. (Read the full article)

Otorrhea is common in children with tympanostomy tubes: annually, 2 of 3 children develop 1 or more episodes. Antibiotic-glucocorticoid eardrops are the most effective treatment in both the short- and long-term.

Treatment with antibiotic-glucocorticoid eardrops costs less than oral antibiotics and initial observation in children with tympanostomy tubes who develop otorrhea. Non–health care costs constitute a substantial proportion of the total costs of this condition. (Read the full article)

Indian Railways Catering and Tourism Corporation’s premier luxury train, the Maharajas Express, is all set to run its first Indian Panorama journey of the 2012-13 season tomorrow. The train will depart from the Safdarjung Railway Station tomorrow at 2200 hours. All guests have been...

Curiosity's project scientist at NASA's Jet Propulsion Laboratory Ashwin Vasavada said that it is the first time that NASA has dedicated its operations to capture a 360-degree panorama.

ABSTRACT

Mitochondrial Ca²⁺ transport mediated by the uniporter complex (MCUC) plays a key role in the regulation of cell bioenergetics in both trypanosomes and mammals. Here we report that Trypanosoma brucei MCU (TbMCU) subunits interact with subunit c of the mitochondrial ATP synthase (ATPc), as determined by coimmunoprecipitation and split-ubiquitin membrane-based yeast two-hybrid (MYTH) assays. Mutagenesis analysis in combination with MYTH assays suggested that transmembrane helices (TMHs) are determinants of this specific interaction. In situ tagging, followed by immunoprecipitation and immunofluorescence microscopy, revealed that T. brucei ATPc (TbATPc) coimmunoprecipitates with TbMCUC subunits and colocalizes with them to the mitochondria. Blue native PAGE and immunodetection analyses indicated that the TbMCUC is present together with the ATP synthase in a large protein complex with a molecular weight of approximately 900 kDa. Ablation of the TbMCUC subunits by RNA interference (RNAi) significantly increased the AMP/ATP ratio, revealing the downregulation of ATP production in the cells. Interestingly, the direct physical MCU-ATPc interaction is conserved in Trypanosoma cruzi and human cells. Specific interaction between human MCU (HsMCU) and human ATPc (HsATPc) was confirmed in vitro by mutagenesis and MYTH assays and in vivo by coimmunoprecipitation. In summary, our study has identified that MCU complex physically interacts with mitochondrial ATP synthase, possibly forming an MCUC-ATP megacomplex that couples ADP and P_i transport with ATP synthesis, a process that is stimulated by Ca²⁺ in trypanosomes and human cells.

IMPORTANCE The mitochondrial calcium uniporter (MCU) is essential for the regulation of oxidative phosphorylation in mammalian cells, and we have shown that in Trypanosoma brucei, the etiologic agent of sleeping sickness, this channel is essential for its survival and infectivity. Here we reveal that that Trypanosoma brucei MCU subunits interact with subunit c of the mitochondrial ATP synthase (ATPc). Interestingly, the direct physical MCU-ATPc interaction is conserved in T. cruzi and human cells.

Midori Ishii and Bungo Akiyoshi

The kinetochore is a macromolecular protein complex that drives chromosome segregation in eukaryotes. Unlike most eukaryotes that have canonical kinetochore proteins, evolutionarily divergent kinetoplastids, such as Trypanosoma brucei, have unconventional kinetochore proteins. T. brucei also lacks a canonical spindle checkpoint system, and it therefore remains unknown how mitotic progression is regulated in this organism. Here, we characterized, in the procyclic form of T. brucei, two paralogous kinetochore proteins with a CLK-like kinase domain, KKT10 and KKT19, which localize at kinetochores in metaphase but disappear at the onset of anaphase. We found that these proteins are functionally redundant. Double knockdown of KKT10 and KKT19 led to a significant delay in the metaphase to anaphase transition. We also found that phosphorylation of two kinetochore proteins, KKT4 and KKT7, depended on KKT10 and KKT19 in vivo. Finally, we showed that the N-terminal part of KKT7 directly interacts with KKT10 and that kinetochore localization of KKT10 depends not only on KKT7 but also on the KKT8 complex. Our results reveal that kinetochore localization of KKT10 and KKT19 is tightly controlled to regulate the metaphase to anaphase transition in T. brucei.

This article has an associated First Person interview with the first author of the paper.