Heat shock factor 1 (HSF1) regulates cellular adaptation to challenges such as heat shock and oxidative and proteotoxic stresses. We have recently reported a previously unappreciated role for HSF1 in the regulation of energy metabolism in fat tissues; however, whether HSF1 is differentially expressed in adipose depots and how its levels are regulated in fat tissues remain unclear. Here, we show that HSF1 levels are higher in brown and subcutaneous fat tissues than in those in the visceral depot and that HSF1 is more abundant in differentiated, thermogenic adipocytes. Gene expression experiments indicated that HSF1 is transcriptionally regulated in fat by agents that modulate cAMP levels, by cold exposure, and by pharmacological stimulation of β-adrenergic signaling. An in silico promoter analysis helped identify a putative response element for activating transcription factor 3 (ATF3) at −258 to −250 base pairs from the HSF1 transcriptional start site, and electrophoretic mobility shift and ChIP assays confirmed ATF3 binding to this sequence. Furthermore, functional assays disclosed that ATF3 is necessary and sufficient for HSF1 regulation. Detailed gene expression analysis revealed that ATF3 is one of the most highly induced ATFs in thermogenic tissues of mice exposed to cold temperatures or treated with the β-adrenergic receptor agonist CL316,243 and that its expression is induced by modulators of cAMP levels in isolated adipocytes. To the best of our knowledge, our results show for the first time that HSF1 is transcriptionally controlled by ATF3 in response to classic stimuli that promote heat generation in thermogenic tissues.

Alumni inspired by the scientific response to the SARS outbreak are developing a platform to help researchers collaborate on global challenges.

(Institute for Operations Research and the Management Sciences) New research to be published in the INFORMS journal Management Science has found popular rideshare platforms exhibit racial and other biases that penalize under-represented minorities and others seeking to use their services.

Heat shock factor 1 (HSF1) regulates cellular adaptation to challenges such as heat shock and oxidative and proteotoxic stresses. We have recently reported a previously unappreciated role for HSF1 in the regulation of energy metabolism in fat tissues; however, whether HSF1 is differentially expressed in adipose depots and how its levels are regulated in fat tissues remain unclear. Here, we show that HSF1 levels are higher in brown and subcutaneous fat tissues than in those in the visceral depot and that HSF1 is more abundant in differentiated, thermogenic adipocytes. Gene expression experiments indicated that HSF1 is transcriptionally regulated in fat by agents that modulate cAMP levels, by cold exposure, and by pharmacological stimulation of β-adrenergic signaling. An in silico promoter analysis helped identify a putative response element for activating transcription factor 3 (ATF3) at −258 to −250 base pairs from the HSF1 transcriptional start site, and electrophoretic mobility shift and ChIP assays confirmed ATF3 binding to this sequence. Furthermore, functional assays disclosed that ATF3 is necessary and sufficient for HSF1 regulation. Detailed gene expression analysis revealed that ATF3 is one of the most highly induced ATFs in thermogenic tissues of mice exposed to cold temperatures or treated with the β-adrenergic receptor agonist CL316,243 and that its expression is induced by modulators of cAMP levels in isolated adipocytes. To the best of our knowledge, our results show for the first time that HSF1 is transcriptionally controlled by ATF3 in response to classic stimuli that promote heat generation in thermogenic tissues.

We finally get the IoT acronym the pod has been waiting for as we discuss Bitcoin and 17th century Dutch finance. Charlotte Jee informs Henry Burrell, David Price and Scott Carey.

Scott then leads us down the winding road of the so-called platform wars, in a week when Google blocked the Amazon Echo Show from streaming YouTube. Is this a bump in the road or will services be cut from rival hardware?

And finally, led by David, the pod discusses its favourite tech of the year and what you might consider buying for friends and family for Christmas.

See acast.com/privacy for privacy and opt-out information.

Bile acids (BAs) serve multiple biological functions, ranging from the absorption of lipids and fat-soluble vitamins to serving as signaling molecules through the direct activation of dedicated cellular receptors. Synthesized by both host and microbial pathways, BAs are increasingly understood as participating in the regulation of numerous pathways relevant to metabolic diseases, including lipid and glucose metabolism, energy expenditure, and inflammation. Quantitative analyses of BAs in biological matrices can be problematic due to their unusual and diverse physicochemical properties, making optimization of a method that shows good accuracy, precision, efficiency of extraction, and minimized matrix effects across structurally distinct human and murine BAs challenging. Herein we develop and clinically validate a stable-isotope-dilution LC/MS/MS method for the quantitative analysis of numerous primary and secondary BAs in both human and mouse biological matrices. We also utilize this tool to investigate gut microbiota participation in the generation of structurally specific BAs in both humans and mice. We examine circulating levels of specific BAs and in a clinical case-control study of age- and gender-matched type 2 diabetes mellitus (T2DM) versus nondiabetics. BAs whose circulating levels are associated with T2DM include numerous 12α-hydroxyl BAs (taurocholic acid, taurodeoxycholic acid, glycodeoxycholic acid, deoxycholic acid, and 3-ketodeoxycholic acid), while taurohyodeoxycholic acid was negatively associated with diabetes. The LC/MS/MS-based platform described should serve as a robust, high-throughput investigative tool for studying the potential involvement of structurally specific BAs and the gut microbiome on both physiological and disease processes.

2 July 2014 , Volume 90, Number 4

Raytheon was awarded a $325 million contract for repair of the Advanced Targeting Forward Looking Infrared System in the F/A-18 Super Hornet aircraft Tuesday, according to the Pentagon.

London (No. 4, Hanover Street) : Published ... for the proprietor by T.G. March ; [London] (Threadneedle Street) : Sold also by F. G. Moon ; [London] (Strand) : [Sold also by] Ackermann & Co., April 10, 1842 ([London?] : Printed by R. Lloyd)

Youyi Zhang, Jeffrey S. Morris, Shivali Narang Aerry, Arvind U. K. Rao, Veerabhadran Baladandayuthapani.

Source: The Annals of Applied Statistics, Volume 13, Number 3, 1957--1988.

Abstract:
Technological innovations have produced large multi-modal datasets that include imaging and multi-platform genomics data. Integrative analyses of such data have the potential to reveal important biological and clinical insights into complex diseases like cancer. In this paper, we present Bayesian approaches for integrative analysis of radiological imaging and multi-platform genomic data, where-in our goals are to simultaneously identify genomic and radiomic, that is, radiology-based imaging markers, along with the latent associations between these two modalities, and to detect the overall prognostic relevance of the combined markers. For this task, we propose Radio-iBAG: Radiomics-based Integrative Bayesian Analysis of Multiplatform Genomic Data , a multi-scale Bayesian hierarchical model that involves several innovative strategies: it incorporates integrative analysis of multi-platform genomic data sets to capture fundamental biological relationships; explores the associations between radiomic markers accompanying genomic information with clinical outcomes; and detects genomic and radiomic markers associated with clinical prognosis. We also introduce the use of sparse Principal Component Analysis (sPCA) to extract a sparse set of approximately orthogonal meta-features each containing information from a set of related individual radiomic features, reducing dimensionality and combining like features. Our methods are motivated by and applied to The Cancer Genome Atlas glioblastoma multiforme data set, where-in we integrate magnetic resonance imaging-based biomarkers along with genomic, epigenomic and transcriptomic data. Our model identifies important magnetic resonance imaging features and the associated genomic platforms that are related with patient survival times.

If you are looking for a well-designed Linux distro that is far from mainstream, loaded with performance features not found elsewhere, check out the 2020 upgrade of the MakuluLinux Core distro. It could change your perspective on what a daily computing driver should offer. Developer Jacque Montague Raymer recently released the 2020 edition of MakuluLinux Core OS.

The post 3DEXPERIENCE Platform Now Available for Students! appeared first on SOLIDWORKS Education Blog.

Miraculous healing from sudden paralysis gives an OM worker opportunity to preach the gospel in a community.

In 1999, national bitterness and divisions were set aside on board Doulos, which facilitated an historic reconciliation after conflict in the Pacific islands.

A look at the two major political parties' platforms since the first charter school law was passed shows how Democrats' positions on school choice have evolved, including increased calls for accountability.

Buy a universal purchase app for macOS or iOS and use it across all your devices on all Apple platforms including iPadOS, tvOS, and watchOS devices.

Social networks are calling themselves platforms rather than publishers to skirt around legal issues, and it has to stop.

With TikTok, Dailyhunt, ShareChat and even OTT players Zee5, Amazon Prime and Hoichoi letting users post, watch and share content in the language of their choice, few are spending considerable time on Facebook.

OTT platforms are partnering with travel agencies, airlines and cruises to tap on-the-go audiences

Online publishers often lack the knowledge and familiarity to leverage online tools to monetise content

As per a report by BARC India and Nielsen, there has been a 30% increase in the time spent on education apps on smartphones since the lockdown

The Silver Lake infusion values it at an equity value of Rs 4.90 lakh crore.

BMW 8 Series line-up will be positioned above the 6 Series Gran Coupe and will come in two variants - the 840i Gran Coupe and the M8 Coupe.

  Golden Hammer Software took a trip earlier this year to New York to attend the Intel Android CodeFest.  We updated Big Mountain Snowboarding, Trick Shot Bowling, and Scribble Worm to run natively...

  The H.265/HEVC standard is becoming more and more popular in the mobile market. Online video providers, mobile Internet users, and broadcast/TV operators are the beneficiaries of this new video revolu...

  Most Android applications, even those based only on scripting and managed languages (Java*, HTML5,...) eventually use middleware features that would benefit from optimization. This article willl di...

  Intel has a vested interest in helping developers provide Android applications that run well (or even best) on Intel architecture. While Intel is working at the community level - optimizing Dalvik Jav...

Cube Sessions offers performances and interviews with prominent underground artists, all staged from within a cube-shaped, neon-lit metal scaffold. Artists include soloists Zeid Hamdan and Lynn Adib as well as bands Adonis and Tanjaret Daghet

Will spearhead innovation in geospatial services

TIME, the test-prep institute, has started online courses. These include CLAT + IPM, BBA and CAT etc.

The rise in Jio’s ARPU (average revenue per user) during Q4FY20 was lacklustre, analysts said despite the fairly sharp 14-53% hike in tariffs in early December.

•Dassault Aviation will rely on six Dassault Systèmes industry solution experiences to integrate business processes, improve performance and reduce costs •Deployment marks next step in Dassault Aviation’s digital transformation plan through a platform approach, launched in 2018 •Dassault Systèmes’ 3DEXPERIENCE platform will power artificial intelligence-based application for intelligent enterprise services

•Lockheed Martin deploys the 3DEXPERIENCE platform as an engineering and manufacturing planning toolset •Multi-year collaboration aims to speed timelines and improve efficiencies of next generation products •Digital experience platform approach drives advances in complex, sophisticated aircraft innovation

Medidata Accelerates Launch of myMedidata Platform, Responding to COVID-19

AVideo Platform version 8.1 suffers from a cross site request forgery vulnerability.

IBM Bigfix Platform version 9.5.9.62 suffers from an arbitrary file upload vulnerability as root that can achieve remote code execution.

AVideo Platform version 8.1 suffers from an information disclosure vulnerability that allows for user enumeration.

Energy advisory and certification body DNV GL has launched a new digital platform for renewable energy power purchase agreements.

Join Austrade's webinar to gain insights on the Bangladesh E-commerce market, emerging trends, growth drivers, regulations, route to market and opportunities for partnering with Bangladesh online companies.