free Grain Free Clusters Trail Trek Blend By www.preparedfoods.com Published On :: Wed, 11 Mar 2020 00:00:00 -0400 Bakery On Main is expanding its diverse portfolio of gluten-free cereals and snacks with the addition of a certified Paleo and Keto Grain-Free Cluster Trail Trek Blend snack line. Full Article
free SmartyPants Vitamins New Sugar Free Gummy Multivitamin By www.preparedfoods.com Published On :: Wed, 10 May 2023 06:00:00 -0400 For its most innovative launch to-date, SmartyPants Vitamins announced new SmartyPants Sugar Free Multi & Omegas gummy multivitamins in Kids, Women’s, and Prenatal formats. SmartyPants’ new Sugar Free Multi & Omegas don’t use sugar alcoh Full Article
free Stellar Snacks Grain-Free Superfood Pretzels By www.preparedfoods.com Published On :: Tue, 03 Sep 2024 08:00:00 -0400 Stellar Pretzel Thins marks a launch for the vegan and peanut-free pretzel company as the brand becomes even more inclusive to the everyday consumer, especially those with gluten allergies and health-restrictions. Full Article
free How FDA Labeling Requirements Will Impact Gluten-Free Products in 2021 By www.preparedfoods.com Published On :: Thu, 11 Feb 2021 15:00:00 -0500 If 2020 was the year of supply chain uncertainty due to COVID-19, 2021 may well be the year that transparency in food labeling takes center stage, particularly for gluten-free products. Full Article
free Red Bull Sugarfree Watermelon, Strawberry Apricot By www.preparedfoods.com Published On :: Tue, 13 Aug 2024 08:00:00 -0400 The new varieties will join the original Red Bull Sugarfree and Red Bull Zero in this expansion of zero sugar options. Full Article
free Feel Good Foods Gluten-Free Chicken Soup Dumplings By www.preparedfoods.com Published On :: Thu, 07 Nov 2024 08:00:00 -0500 The dumplings are filled with a mix of tender chicken, savory broth, scallions, and ginger, delivering a comforting, bold flavor. Crafted with no artificial flavors, colors, or sweeteners, they provide a wholesome, allergen-friendly option. Full Article
free New Dust-Free Docking Station By www.foodengineeringmag.com Published On :: Tue, 30 Jan 2024 07:00:00 -0500 Gericke’s dust-free docking station is included as standard equipment on the bulk bag unloader. Full Article
free FDA Releases Results of Sampling of Retail Dairy-Free Dark Chocolate and Chocolate-Containing Products for Milk Allergen By www.foodengineeringmag.com Published On :: Mon, 07 Oct 2024 00:00:00 -0400 The sampling was conducted to better understand the extent to which these products potentially contain unintended milk at levels that may be hazardous to people with milk allergy. Full Article
free Holiday Inn: Milton Keynes East M1, Jct.14 - Free 5k Bonus Points By www.flyertalk.com Published On :: Sat, 09 Nov 2024 19:13:10 GMT There are a few nights at the Holiday Inn: Milton Keynes East M1, Jct.14 where the 5k IHG Bonus Points rate is either a few pounds less than the discount rate or the same (based on a lower room category being available). November 14, 17, 21, 28,... Full Article Hotel Deals
free Which airport has the cheapest duty free alcohol? By www.flyertalk.com Published On :: Wed, 23 Oct 2024 18:54:25 GMT For example, which airport has cheapest MARTELL CORDON BL.COG.40% 1L? Btw I will visit soon ZRH, CPH, FRA and SIN. Any suggestion is highly appreciated. Full Article Information Desk
free Taylor Adhesive's 501 Solvent-Free Epoxy Flooring Adhesive Ideal for Resilient By www.floortrendsmag.com Published On :: Mon, 12 Aug 2024 08:00:00 -0400 Taylor Adhesives 501 is a fast-setting, two-part, low-VOC epoxy system used to install vinyl and rubber flooring, including hard-to-stick recycled products. Full Article
free Linde to Showcase Cryogenic Freezing Capabilities at PACK EXPO By www.foodengineeringmag.com Published On :: Thu, 12 Sep 2024 11:19:00 -0400 Linde works with food processors to optimize their use of liquid nitrogen and/or liquid carbon dioxide in cryogenic freezing and chilling applications. Full Article
free Compact Freezer for Energy Efficiency By www.foodengineeringmag.com Published On :: Tue, 01 Oct 2024 10:43:28 -0400 The ADVANTEC Narrow brings all of the capacity and functionality benefits of the original ADVANTEC Impingement Freeze, but with innovations aimed at reducing its footprint and alleviating the pressures of high cryogenic gas prices on companies. Full Article
free ABB Updates Freelance Distributed Control System By www.foodengineeringmag.com Published On :: Wed, 02 Oct 2024 11:09:00 -0400 Freelance 2024 facilitates enhanced connectivity, faster data transfer, more precise control and monitoring of data together with an improved network performance. Full Article
free GEA Debuts RAY Plus Freeze Dryers By www.foodengineeringmag.com Published On :: Fri, 01 Nov 2024 09:49:00 -0400 The new RAY Plus series delivers benefits in terms of energy efficiency and hygiene, providing a more flexible and efficient solution for the food industry. Full Article
free Vacuum Thermodynamics in our Freeze-Dried Universe: A Christmas Study By www.foodengineeringmag.com Published On :: Mon, 18 Dec 2023 07:00:00 -0500 The lessons learned from the principles and subsystems in play can and should be applied broadly to your own industrial vacuum system, whether freeze-drying is your game or not. Full Article
free Cal-Maine Foods, Inc. Announces Expansion Plans for Additional Cage-Free Production Capabilities By www.foodengineeringmag.com Published On :: Tue, 08 Oct 2024 09:32:55 -0400 The projects will include the addition of five new cage-free layer houses across its locations in Florida, Georgia, Utah and Texas. Full Article
free Inclusion, social capital and space within an English secondary free school. By ezproxy.scu.edu.au Published On :: Sun, 01 Aug 2021 00:00:00 -0400 Children's Geographies; 08/01/2021(AN 152310089); ISSN: 14733285Academic Search Premier Full Article ENGLAND FREE schools SOCIAL space SOCIAL capital SECONDARY schools SOCIAL integration PUBLIC spaces ANALYTIC spaces
free The relationship between young children's transitions and power: 'Why are all the doors locked? I don't feel free ... I am not in charge of me anymore'. By ezproxy.scu.edu.au Published On :: Sat, 01 Oct 2022 00:00:00 -0400 Children's Geographies; 10/01/2022(AN 159948778); ISSN: 14733285Academic Search Premier Full Article INSTITUTIONAL care of children PARTICIPANT observation SOCIAL reality PRIMARY schools PUBLIC spaces
free Constructing and governing freedom – physical environments as a discursive practice in Swedish early childhood education. By ezproxy.scu.edu.au Published On :: Tue, 01 Oct 2024 00:00:00 -0400 Children's Geographies; 10/01/2024(AN 180134753); ISSN: 14733285Academic Search Premier Full Article EARLY childhood education PRESCHOOL teachers DISCURSIVE practices PRESCHOOLS LIBERTY
free Campus Free Speech: A Pocket Guide By ifp.nyu.edu Published On :: Fri, 01 Nov 2024 21:31:32 +0000 The post Campus Free Speech: A Pocket Guide was curated by information for practice. Full Article Monographs & Edited Collections
free Free Talk Group Motivational Interviewing (MI) for Teens By ifp.nyu.edu Published On :: Tue, 05 Nov 2024 01:18:33 +0000 The post Free Talk Group Motivational Interviewing (MI) for Teens was curated by information for practice. Full Article Guidelines Plus
free NICE-recommended digital therapies for depression and anxiety could free up thousands of NHS therapist hours By ifp.nyu.edu Published On :: Tue, 15 Oct 2024 19:24:41 +0000 The post NICE-recommended digital therapies for depression and anxiety could free up thousands of NHS therapist hours was curated by information for practice. Full Article Infographics
free Freedom and voting power By ifp.nyu.edu Published On :: Mon, 04 Nov 2024 15:24:18 +0000 Abstract This paper proposes a method of evaluating elections in terms of freedom of choice. It evaluates voting institutions in terms of their allocation of control. Formally, the paper develops the symmetric power order, a measure of voting power for multicandidate elections. The measure generalizes standard pivotality-based voting power measures for binary elections, such as […] The post Freedom and voting power was curated by information for practice. Full Article Journal Article Abstracts
free SOUTH EASTERN FREEWAY, CRAFERS WEST (Vehicle Leaking Fuel) By www.cfs.sa.gov.au Published On :: Wed, 13 Nov 2024 23:42:03 +1030 First Reported: Wednesday, 13 Nov 2024 23:28:00Status: GOINGRegion: 1 Full Article
free Good Morning, News: Most Beautiful Bat Found in Oregon, Young Thug Walks Free, and a Terrifying Shooting at Vancouver Mall By www.portlandmercury.com Published On :: Fri, 01 Nov 2024 08:36:00 -0700 by Suzette Smith The Mercury provides its readers with interesting and useful news & culture reporting every single day. If you appreciate that, consider making a small monthly contribution to support our editorial team. If you read something you like, something you don't like but are glad to know about, and/or something you can't find anywhere else consider a one-time tip. It all goes in the same pot and it all goes to the editorial team. Thanks for your support! Good Morning, Portland! When they told me a bat could be beautiful I didn't believe, but now I believe. IN LOCAL NEWS:• As October comes to a close, so too does the Bureau of Land Management’s annual Bat Beauty Contest, which asks where the "most stunning bat photographed on BLM public lands" can be found? It's Oregon, motherfuckers! For the third year running WE HAVE THE MOST BEAUTIFUL BAT. YES! Despite J. K. Rowling's tarnished-as-hell legacy, we can still celebrate this feisty male hoary bat, Hoary Potter. It's also worth noting that this is the second year a bat photographed by wildlife technician Emma Busk took top beauty honors. Busk wrote, "anyone who knows me knows that I’ve been wanting to photograph a hoary bat,"—a sentiment we can all echo. BLM's most beautiful bat is from Oregon, and I love him. pic.twitter.com/T8qQgsz5kT — Suzette Smith (@suzettesmith) November 1, 2024 • There's also terrible news, unfortunately. Yesterday evening, a shooting at Vancouver Mall, in Vancouver, WA, killed one person and injured two in the building's food court. A witness who spoke to KOIN described "a harrowing scene, as the shopping center was full of kids trick-or-treating." So far, police have said they do not have a suspect in custody and the person may still be armed. They told KOIN they were reviewing surveillance footage, but have not yet released a description of the shooter. • Oh my god, Clark County are you okay? KPTV reports that Clark County Elections Office is warning voters to beware of unofficial ballot boxes. Unofficial ballot boxes are not illegal in Washington! (This also appears to be true of Oregon!) Clark County Elections encouraged voters official ballot return locations. • The Oregonian is returning to broadsheet size, they announced yesterday in a post that did not explain what that is for readers who didn't go to graphic design school / work in at newspaper with some old dude named Phil. Somewhat surprisingly, Willamette Week's Nigel Jaquiss stepped in to clarify. Tabloid is hotdog style fold; broadsheet is burger. Both WW and the Mercury are hotdogs. The New York Times is burger fold. • NPR's Up First weirdly tried to do a report on Vice President Kamala Harris "highlighting Trump's own language and using it against him," as if that's somehow something of which to be critical? Gave me the vapors of the new Hasaan Hates Portland sketch from this week: • In two week's the city's beloved queer documentary festival QDoc will host the Oregon debut of The World According to Allee Willis, which explores the complicated life of a queer "song doctor" who co-wrote not only Earth, Wind and Fire's "September," but the Friends TV show theme. Check out Melissa Locker's review of the film.; she says it's not to be missed! • Did you take this week's local news quiz? View this post on Instagram A post shared by Portland Mercury (@portlandmercury) IN NATIONAL NEWS:• Atlanta rap star Young Thug, born Jeffery Williams, finally walked free last night, after pleading guilty to "six counts, including possession of drugs and firearms... leading a criminal street gang and conspiracy to violate the RICO act, the state’s racketeering law," the New York Times reports. As part of the plea, the judge sentenced Williams to a total sentence of 40 years, but commuted the five years of prison time to the two and a half years that Williams has already served. Williams will remain on probation for 15 years, and will "be required to stay away from metro Atlanta for the first 10 years of his probation." He is also required to take random drug tests, make anti-gang presentations for children's organizations four times a year, perform 100 hours of community service a year, and "refrain from promoting gangs or being around known gang members." The ongoing Young Slime Life (YSL) court proceedings—which caused NYT's Joe Coscarelli to ask "is YSL was a record label, street gang, or both?"—have stretched into what is now the longest trial in Georgia history. Despite three other plea deals this week, it appears the trial will continue with the two remaining co-defendants. • Mark Zuckerberg dressed as Fran Lebowitz for Halloween, and that's just what happened. Mark Zuckerberg dressed as John Wick for Halloween but I thought it was Fran Lebowitz pic.twitter.com/zdXaomOMPR — Meech (@MediumSizeMeech) November 1, 2024 • You're going to see some stuff today about "explosive new audio" recordings where Donald Trump speaks candidly to the reviled Jeffrey Epstein about his White House Staff and his disdain for the position itself. It's only really on the Daily Beast, the podcast of journalist Michael Wolff, and a few other sites. I'd rather wait til a super solid source gets it—seems like maybe they're holding off because they're checking it?—but the story is out there. Don't sound like anything particularly new, but people are saying "October surprise" because they want attention. • Also in weird news about guys we already thought were corrupt, Supreme Court Justice Samuel Alito is apparently pledged to some secretive religious traditionalist organization. That tracks. • Flashback to this masterpiece, which has been floating around the zeitgeist all week. Full Article Good Morning News!
free FREE TICKETS THURSDAY: Enter to Win Free Tix to See Reverend Horton Heat, Des Demonas, Mason Jennings, and MORE! By www.portlandmercury.com Published On :: Thu, 07 Nov 2024 11:24:00 -0800 by Wm. Steven Humphrey Who's ready to have some fun? Well, the Mercury is here to help with FREE TICKETS to see some of Portland's best concerts and events—our way of saying thanks to our great readers and spread the word about some fantastic upcoming performances! (Psst... if you want to say thanks to the Mercury, please consider making a small monthly contribution to keep us alive and kickin'!) And oh boy, do we have some fun events coming at ya this week! CHECK IT OUT! • Enter to WIN FREE TICKETS to see Reverend Horton Heat on February 27 at Crystal Ballroom!Seeing REVEREND HORTON HEAT live is a transformative experience. Flames come off the guitars. Heat singes your skin. There’s nothing like the primal tribal rock & roll transfiguration of a Reverend Horton Heat show. Get your tickets now, or enter to win here! Crystal Ballroom, 1332 W Burnside, Thurs Feb 27, 8 pm, $35-$50, all ages • Enter to WIN FREE TICKETS to see Des Demonas on November 17 at Polaris Hall! A favorite of Henry Rollins and Iggy Pop, Washington, DC’s DES DEMONAS blend post punk, funk, blues, psych rock, Afro beat, and more into their own unique sound — listen LIVE when they share new tunes from their highly-anticipated sophomore LP, APOCALYPTIC BOOM! BOOM! Get your tickets now or enter to win a free pair! Polaris Hall, 635 N Killingsworth Ct, Sun Nov 17, 8 pm, $15, 21+ • Enter to WIN FREE TICKETS to see Karina Rykman on November 15 at Mississippi Studios! She’s opened for Khruangbin, performed on America’s Got Talent, soundchecked with Phish at MSG, and much more — catch dynamic jam rock bassist Karina Rykman LIVE when she returns to Portland! Get your tickets now or enter to win a free pair! Mississippi Studios, 3939 N Mississippi, Fri Nov 15, 9 pm, $20, 21+ • Enter to WIN FREE TICKETS to see Mason Jennings on November 16 at Mississippi Studios! Hawai’i-born, Minneapolis-based folksinger Mason Jennings returns to Mississippi Studios with tunes from his latest record, Holy Dive, and beyond! Get your tickets now or enter to win a free pair! Mississippi Studios, 3939 N Mississippi, Sat Nov 16, 8 pm, $30, 21+ • Enter to WIN FREE TICKETS to see The Bygones on November 17 at Aladdin Theater! Luminous indie folk duo The Bygones share nostalgic songwriting from their debut, self-titled release! Get your tickets now or enter to win a free pair! Aladdin Theater, 3017 SE Milwaukie, Sun Nov 17, 8 pm, $25, all ages • Enter to WIN FREE TICKETS to see Sam Grisman Project on November 16 at Aladdin Theater! A longtime Dawg Trio member with his father David Grisman, Bay Area bass player Sam Grisman upholds the legacy of Dawg music with his new musical project. Don’t miss their return to Portland! Get your tickets now or enter to win a free pair! Aladdin Theater, 3017 SE Milwaukie, Sat Nov 16, 8 pm, $32.50, all ages GOOD LUCK! Winners will be notified on Monday. Check back next week for more FREE TIX from the Mercury! Full Article Music
free Fast free search for all of Shakespeare By feeds.playshakespeare.com Published On :: Thu, 28 Mar 2019 07:37:51 -0400 shearch.me is a free Progressive Web App that searches Shakespeare's plays, poems and sonnets including locations and stage directions. It's quick, works on desktop or mobile, and it uses the wonderful PlayShakespeare texts. Because it's a Progressive Web App, you can use it like a website (just go to shearch.me in a web browser) or you can add it ... Full Article General
free Cool high pressure Monday with freezing temperatures overnight By www.mprnews.org Published On :: Mon, 11 Nov 2024 11:52:00 +0000 Chilly high pressure has settled in behind the weekend system. High temperatures will be cooler Monday and Tuesday, but near normal. The next chance of rain develops Wednesday. Full Article
free ‘It’s the best week’: After being wrongly incarcerated, a Minnesota man is now free By www.mprnews.org Published On :: Wed, 13 Nov 2024 01:41:00 +0000 Edgar Barrientos-Quintana was wrongly convicted in 2009 and sentenced to life in prison without parole for the 2008 murder of Jesse Mickelson in Minneapolis. Full Article
free Christian attorney warns 'tearing down' religious freedom in US is 'spirit of the age' By www.christianpost.com Published On :: Mon, 04 Nov 2024 17:54:11 -0500 An attorney dedicated to upholding religious freedom stated that he is “greatly disturbed” to see what Michigan has become, warning of a “growing hostility” in the state toward the exercise of religion in the public square. Full Article
free Freedom without choice is no true freedom at all By www.christianpost.com Published On :: Fri, 14 Jul 2023 10:31:52 -0400 It's not often I am surprised by comments that are generated by my columns in The Christian Post. So imagine my surprise at being taken aback by some comments generated by my July 4th column. Full Article
free Did a momentous event occur in the predestination vs. free will debate? By www.christianpost.com Published On :: Fri, 21 Jul 2023 09:36:00 -0400 "God in Eternity and Time" has been well received by all segments of the Evangelical community, and its irenic spirit has been much appreciated, drawing accolades from across the Evangelical theological spectrum. Full Article
free Football fans attack Israelis in Amsterdam all night shouting 'Free Palestine!' By english.pravda.ru Published On :: Fri, 08 Nov 2024 15:54:00 +0300 In Amsterdam, people with Palestinian flags attacked Israelis after a football match between local club Ajax and Tel Aviv's Maccabi. Masked men waving Palestinian flags attacked Israelis after the Israeli soccer team lost to Ajax in the Europa League. The attackers were chasing and beating Tel Aviv Maccabi fans throughout the night shouting "Free Palestine!" Click here to see more raw videos from Amsterdam Full Article World
free PervasID’s TrackMaster replaces manual handheld with accurate handsfree real-time location tracking solution By www.retailtechnologyreview.com Published On :: PervasID, provider of passive Radio Frequency Identification (RFID) reader systems for automating inventory and asset tracking, has launched a new advanced version of TrackMaster, claimed to provide greatly enhanced return on investment performance. Full Article RFID Data Capture
free Dehumanising Child-Free Women in Film and TV Gives Misogyny a Stage By www.newswise.com Published On :: Mon, 11 Nov 2024 20:40:25 EST University of South Australia PhD student Belinda Lees has explored how screenwriters could create more nuanced and complex child-free women protagonists in biopics, after uncovering the barrage of often negative portrayals in existing media. Full Article
free S. Korea, US, Japan Launch Massive Freedom Edge Multidomain Exercise By world.kbs.co.kr Published On :: Wed, 13 Nov 2024 15:47:29 +0900 [Politics] : Anchor: South Korea, the United States and Japan are conducting their second trilateral multidomain exercise in international waters south of South Korea’s Jeju Island. The U.S. Navy’s USS George Washington aircraft carrier, South Korea’s ROKS Seoae Ryu Seong-ryong destroyer and Japan’s JS Haguro ...[more...] Full Article Politics
free S. Korea, US, Japan to Hold Second Trilateral Freedom Edge Exercise This Week By world.kbs.co.kr Published On :: Wed, 13 Nov 2024 10:01:47 +0900 [International] : South Korea, the United States and Japan are set to hold their trilateral multidomain Freedom Edge exercise this week. According to South Korea’s Joint Chiefs of Staff(JCS), this year’s second Freedom Edge exercise runs Wednesday to Friday in international waters south of Jeju Island. The three ...[more...] Full Article International
free 12 Freest Countries in the World, According to the HFI By people.howstuffworks.com Published On :: Tue, 12 Nov 2024 10:10:02 -0500 Everyone probably likes to imagine that they live in one of the freest countries in the world, but is "freedom" something that can even be quantified? Is there a definitive way to say that my country is more free than your country? Full Article
free Freeway vs. Highway: Can You Tell the Difference? By auto.howstuffworks.com Published On :: Wed, 03 Apr 2024 11:42:00 -0400 Highways and freeways can both be designed with multiple lanes, entrance and exit ramps and limited traffic control to reduce congestion and improve driver safety. However, some small highway vs. freeway distinctions place them in separate categories. Full Article
free 'Free Cholsoo Lee' Named Outstanding Historical Documentary at 45th Emmys By world.kbs.co.kr Published On :: Sat, 28 Sep 2024 15:59:20 +0900 [Culture] : A documentary about a 1970s campaign in the United States to release a Korean immigrant, who was wrongfully convicted of murder, from prison was honored at this year's Emmy Awards. According to the National Academy of Television Arts and Sciences(NATAS), the documentary, titled "Free Cholsoo ...[more...] Full Article Culture
free How to Unlock Your iPhone: A Step-by-step Guide to Freedom By electronics.howstuffworks.com Published On :: Tue, 23 Jul 2024 11:54:11 -0400 The ability to unlock your iPhone means that you can use it with different carriers. To unlock your iPhone, use these steps. Full Article
free Emotional Cancer Meets Free-Spirited Aquarius: Compatibility Breakdown By entertainment.howstuffworks.com Published On :: Fri, 08 Nov 2024 05:30:03 -0500 Discover the compatibility between Cancer and Aquarius in love, friendship, and relationships. Learn how their emotional and intellectual traits align or clash. Full Article
free Crystal structure predictions for molecules with soft degrees of freedom using intermonomer force fields derived from first principles By journals.iucr.org Published On :: Full Article text
free Polymorph sampling with coupling to extended variables: enhanced sampling of polymorph energy landscapes and free energy perturbation of polymorph ensembles By journals.iucr.org Published On :: 2024-10-15 A novel approach to computationally enhance the sampling of molecular crystal structures is proposed and tested. This method is based on the use of extended variables coupled to a Monte Carlo based crystal polymorph generator. Inspired by the established technique of quasi-random sampling of polymorphs using the rigid molecule constraint, this approach represents molecular clusters as extended variables within a thermal reservoir. Polymorph unit-cell variables are generated using pseudo-random sampling. Within this framework, a harmonic coupling between the extended variables and polymorph configurations is established. The extended variables remain fixed during the inner loop dedicated to polymorph sampling, enforcing a stepwise propagation of the extended variables to maintain system exploration. The final processing step results in a polymorph energy landscape, where the raw structures sampled to create the extended variable trajectory are re-optimized without the thermal coupling term. The foundational principles of this approach are described and its effectiveness using both a Metropolis Monte Carlo type algorithm and modifications that incorporate replica exchange is demonstrated. A comparison is provided with pseudo-random sampling of polymorphs for the molecule coumarin. The choice to test a design of this algorithm as relevant for enhanced sampling of crystal structures was due to the obvious relation between molecular structure variables and corresponding crystal polymorphs as representative of the inherent vapor to crystal transitions that exist in nature. Additionally, it is shown that the trajectories of extended variables can be harnessed to extract fluctuation properties that can lead to valuable insights. A novel thermodynamic variable is introduced: the free energy difference between ensembles of Z' = 1 and Z' = 2 crystal polymorphs. Full Article text
free X-ray crystal structure of a designed rigidified imaging scaffold in the ligand-free conformation By journals.iucr.org Published On :: 2024-05-20 Imaging scaffolds composed of designed protein cages fused to designed ankyrin repeat proteins (DARPins) have enabled the structure determination of small proteins by cryogenic electron microscopy (cryo-EM). One particularly well characterized scaffold type is a symmetric tetrahedral assembly composed of 24 subunits, 12 A and 12 B, which has three cargo-binding DARPins positioned on each vertex. Here, the X-ray crystal structure of a representative tetrahedral scaffold in the apo state is reported at 3.8 Å resolution. The X-ray crystal structure complements recent cryo-EM findings on a closely related scaffold, while also suggesting potential utility for crystallographic investigations. As observed in this crystal structure, one of the three DARPins, which serve as modular adaptors for binding diverse `cargo' proteins, present on each of the vertices is oriented towards a large solvent channel. The crystal lattice is unusually porous, suggesting that it may be possible to soak crystals of the scaffold with small (≤30 kDa) protein cargo ligands and subsequently determine cage–cargo structures via X-ray crystallography. The results suggest the possibility that cryo-EM scaffolds may be repurposed for structure determination by X-ray crystallography, thus extending the utility of electron-microscopy scaffold designs for alternative structural biology applications. Full Article text
free Real-time analysis of liquid-jet sample-delivery stability for an X-ray free-electron laser using machine vision By journals.iucr.org Published On :: This paper describes real-time statistical analysis of liquid jet images for SFX experiments at the European XFEL. This analysis forms one part of the automated jet re-alignment system for SFX experiments at the SPB/SFX instrument of European XFEL. Full Article text
free Correlation of refractive index based and THz streaking arrival time tools for a hard X-ray free-electron laser By journals.iucr.org Published On :: 2024-01-22 To fully exploit ultra-short X-ray pulse durations routinely available at X-ray free-electron lasers to follow out-of-equilibrium dynamics, inherent arrival time fluctuations of the X-ray pulse with an external perturbing laser pulse need to be measured. In this work, two methods of arrival time measurement were compared to measure the arrival time jitter of hard X-ray pulses. The methods were photoelectron streaking by a THz field and a transient refractive index change of a semiconductor. The methods were validated by shot-to-shot correction of a pump–probe transient reflectivity measurement. An ultimate shot-to-shot full width at half-maximum error between the devices of 19.2 ± 0.1 fs was measured. Full Article text
free Ultrashort large-bandwidth X-ray free-electron laser generation with a dielectric-lined waveguide By journals.iucr.org Published On :: 2024-02-09 Large-bandwidth pulses produced by cutting-edge X-ray free-electron lasers (FELs) are of great importance in research fields like material science and biology. In this paper, a new method to generate high-power ultrashort FEL pulses with tunable spectral bandwidth with spectral coherence using a dielectric-lined waveguide without interfering operation of linacs is proposed. By exploiting the passive and dephasingless wakefield at terahertz frequency excited by the beam, stable energy modulation can be achieved in the electron beam and large-bandwidth high-intensity soft X-ray radiation can be generated. Three-dimensional start-to-end simulations have been carried out and the results show that coherent radiation pulses with duration of a few femtoseconds and bandwidths ranging from 1.01% to 2.16% can be achieved by changing the undulator taper profile. Full Article text
free X-ray beam diagnostics at the MID instrument of the European X-ray Free-Electron Laser Facility By journals.iucr.org Published On :: 2024-04-08 The Materials Imaging and Dynamics (MID) instrument at the European X-ray Free-Electron Laser Facility (EuXFEL) is equipped with a multipurpose diagnostic end-station (DES) at the end of the instrument. The imager unit in DES is a key tool for aligning the beam to a standard trajectory and for adjusting optical elements such as focusing lenses or the split-and-delay line. Furthermore, the DES features a bent-diamond-crystal spectrometer to disperse the spectrum of the direct beam to a line detector. This enables pulse-resolved characterization of the EuXFEL spectrum to provide X-ray energy calibration, and the spectrometer is particularly useful in commissioning special modes of the accelerator. Together with diamond-based intensity monitors, the imager and spectrometer form the DES unit which also contains a heavy-duty beamstop at the end of the MID instrument. Here, we describe the setup in detail and provide exemplary beam diagnostic results. Full Article text