Renal Cell Carcinoma (RCC) is one of the most commonly diagnosed cancers worldwide with research efforts dramatically improving understanding of the biology of the disease. To investigate the role of the immune system in treatment-naïve clear cell Renal Cell Carcinoma (ccRCC), we interrogated the immune infiltrate in patient-matched ccRCC tumor samples, benign normal adjacent tissue (NAT) and peripheral blood mononuclear cells (PBMCs isolated from whole blood, focusing our attention on the myeloid cell infiltrate. Using flow cytometric, MS, and ExCYT analysis, we discovered unique myeloid populations in PBMCs across patient samples. Furthermore, normal adjacent tissues and ccRCC tissues contained numerous myeloid populations with a unique signature for both tissues. Enrichment of the immune cell (CD45⁺) fraction and subsequent gene expression analysis revealed a number of myeloid-related genes that were differentially expressed. These data provide evidence, for the first time, of an immunosuppressive and pro-tumorigenic role of myeloid cells in early, clinically localized ccRCC. The identification of a number of immune proteins for therapeutic targeting provides a rationale for investigation into the potential efficacy of earlier intervention with single-agent or combination immunotherapy for ccRCC.

Paramphistomosis, caused by the rumen fluke, Calicophoron daubneyi, is a parasitic infection of ruminant livestock which has seen a rapid rise in prevalence throughout Western Europe in recent years. Following ingestion of metacercariae (parasite cysts) by the mammalian host, newly-excysted juveniles (NEJs) emerge and invade the duodenal submucosa which causes significant pathology in heavy infections. The immature larvae then migrate upwards, along the gastrointestinal tract, and enter the rumen where they mature and begin to produce eggs. Despite their emergence, and sporadic outbreaks of acute disease, we know little about the molecular mechanisms used by C. daubneyi to establish infection, acquire nutrients and to avoid the host immune response. Here, transcriptome analysis of four intra-mammalian life-cycle stages, integrated with secretome analysis of the NEJ and adult parasites (responsible for acute and chronic disease respectively), revealed how the expression and secretion of selected families of virulence factors and immunomodulators are regulated in accordance with fluke development and migration. Our data show that whilst a family of cathepsins B with varying S2 sub-site residues (indicating distinct substrate specificities) are differentially secreted by NEJs and adult flukes, cathepsins L and F are secreted in low abundance by NEJs only. We found that C. daubneyi has an expanded family of aspartic peptidases, which is up-regulated in adult worms, although they are underrepresented in the secretome. The most abundant proteins in adult fluke secretions were helminth defence molecules (HDMs) that likely establish an immune environment permissive to fluke survival and/or neutralise pathogen-associated molecular patterns (PAMPs) such as bacterial lipopolysaccharide in the microbiome-rich rumen. The distinct collection of molecules secreted by C. daubneyi allowed the development of the first coproantigen-based ELISA for paramphistomosis which, importantly, did not recognise antigens from other helminths commonly found as co-infections with rumen fluke.

One of the most difficult problems with quantum computing relates to increasing the size of the quantum computer. Researchers globally are seeking to solve this “challenge of scale.” To bring […]

The post Recipe for Scaling: ARQUIN Framework for Simulating a Distributed Quantum Computing System appeared first on HPCwire.

Actor Leonardo DiCaprio turns 50 and actor Calista Flockhart turns 60, among the famous birthdays for Nov. 11.

A bill approved in both houses of the California legislature would allow certified teachers six paid weeks of maternity leave.

Along with winter vegetables, STEM is blooming in Imperial County. Dennis and Daniel Gibbs are growing young scientists by transplanting the scientific method to the second grade.

Public schools in this country are on the threshold of a new era if the results of a California poll are any indication.

Policy Analysis for California Education has been a premier ed policy organization for three decades. As Daisy Gonzales writes, it is in the forefront of shaping current reforms and interpreting them for audiences such as 'On California.'

A California organization is tasked with giving out $9.4 million in grants to local programs across the state to solve shortages.

Teachers unions are scapegoats for low student performance.

Next year's California election will be both a referendum on the massive changes in education finance and testing enacted during Jerry Brown governorship and a test of the political coalition that made those changes possible.

California joins a growing number of cities and states hoping to spotlight which students do and do not have access to high-quality arts education.

As the number of charter schools continues to grow, voters in California will be forced to examine their largess.

A read of California's news outlets makes clear that the state is not going to accept President-elect Donald Trump's policies without a fight, particularly those on immigration.

Michael Kirst will be stepping down from his job as president of the California School Board when Gov. Jerry Brown's tenure is over, Kirst announced this week. Kirst, who is an emeritus professor education and business administration at Stanford University, was first appointed to the state board bac

The blessings of season to you all! We will be back in the New Year. Christmas thoughts follow.

What's taking place in California is nothing less than a quiet revolution in education.

California just made school accountability much more complicated. And that's good.

Five California educators last week filed a lawsuit seeking to stop the state's top teachers' union from collecting dues through mandatory paycheck deductions.

This Quality Counts 2019 Highlights Report captures all the data you need to assess your state's performance on key educational outcomes.

Democrat Gavin Newsom, who takes office Jan. 7, plans to expand full-day kindergarten and child-care offerings in the state, according to media reports.

The bill would have given public school teachers at least six weeks of paid maternity leave.

This Quality Counts 2020 Highlights Report captures all the data you need to assess your state's performance on key educational outcomes.

U.S. Secretary of Education Betsy DeVos green-lighted California and Utah's plans to implement the Every Student Succeed Act. That means just one state is still waiting: Florida.

While thousands of students in wildfire-ravaged Northern California resumed classes last week, thousands of others in Alaska stayed home after a 7.0 magnitude earthquake struck Nov. 30.

NOAA designated the Chumash Heritage National Marine Sanctuary this month, following a decade of advocacy by supporters. The protected site will be finalized after a 45-day review period

The species may have been carried to the Sacramento–San Joaquin Delta in ballast water on ships

SolidWorks CAD Software Enables Engineering Students to Fit ‘CubeSat’ Components Into a Four-Inch Square Box

New design increases user leverage and mobility, and keeps hands clean

As schools apply more scrutiny to the methods and materials they use to teach early reading, educators and parents in some states have started to form new advocacy efforts—trying to pressure states and districts to adopt new approaches to teacher training and evaluating materials.

The U.S. Court of Appeals for the 9th Circuit turned aside a challenge to California's history and social science content standards and curriculum framework.

Delaware's three-person Type 6 engine crew is now fighting the 2,000-acre Dexter Fire in the Inyo National Forest of southern California. Reported on July 12, the lightning-caused blaze is actively burning in brush and timber with only 13 percent containment. Leading the crew is the Delaware Forest Service's southern regional forester and engine boss Erich Burkentine of Milton, along with engine boss trainee Nikki Testa of Newark, and firefighter Hunter Melson of Milford. The crew mobilized on July 13 and is scheduled for a 21-day assignment. Delaware's crew is facing high temperatures, low relative humidity, and gusty afternoon winds on the Dexter Fire as they are conducting initial attack, digging fire line, and felling trees.

The Delaware Forest Service mobilized a 19-person wildfire crew from Blackbird State Forest to northern California. The crew will travel cross-country to join other wildfire crews to battle blazes as the number of wildfires in the area continue to grow, sparked by hot, dry, and windy weather combined with an unstable atmosphere, creating lightning-caused fires.

The Delaware Forest Service mobilized a 3-person wildland fire engine crew from Blackbird State Forest on July 28th for a fire assignment to Wawona, California. Working with the Yosemite National Park’s Emergency Communication Center and various agencies from all over the country, the crew will be working on patrolling and isolating new fire starts due to human activity and lightning strikes.

WILMINGTON, Del. – As the first year of grantmaking comes to a close, Co-Chairs Attorney General Kathy Jennings and Lt. Governor Bethany Hall-Long shared the results of the third-party report from independent contractor Social Contract with recommendations to maximize the impact of the governing body overseeing Delaware’s opioid settlement funds.     The report, Enhancing Use […]

Allegro part of DPI does not support scaling above 150%

Hello everyone,

I hope you're all doing well. I’ve set up two testbenches (TB1 and TB2) for my Design Under Test (DUT) using Cadence IC6.1.8-64b.500.21 tools, as shown in the attached figure. The DUT has multiple views available: schematic, Calibre, Maestro, and Symbol, and each testbench uses the same DUT in different scenarios. Currently, I have to manually switch between these views, but I would like to streamline this process.

My goal is to use a single config view that allows me to switch between the schematic and the extracted (Calibre) views. Ideally, I would like to have a configuration file where making changes once would update both testbenches (TB1 and TB2) automatically. In other words, when I modify one config, both testbenches should reflect this update for a single simulation run.

I would really appreciate it if you could guide me on the following:

1. How to create a config view for my DUT that can be used to easily switch between the schematic and extracted views, impacting both TB1 and TB2.
2. Where to specify view priorities or other settings to control which view is used during simulation.
3. Best practices for using a config file in this scenario, so that it ensures consistency across multiple testbenches.

Please refer to the attached figure to get a better understanding of the setup I’m using, where both TB1 and TB2 include the same DUT with multiple available views.

Thank you so much for your time and assistance!

Do you know if it is possible to define localized back etching (LBE) in EMX? It should be associated to a layer which defines the holes done in the substrate. I've not found any reference for this in the .proc syntax. 

--> Answer found. This is not possible because EMX considers the same dielectric in all x-y plane

hello

is there a way to scale a footprint of a symbol?

scaling a footprint means moving all the pad locations of the symbol by +x% or -x%. 

regards

masa

Lucid Motors is pitching its Air electric sedan as a potential police cruiser. On Sunday the automaker posted photos of an Air decked out in police equipment on X (formerly Twitter). Lucid said the car had recently participated in California Highway Patrol testing, but did not provide any other details. We've reached out to Lucid with questions...

The Federal Energy Regulatory Commission has received two applications for preliminary permits for a pumped storage project at Lake Elsinore in California.

The California Public Utilities Commission (CPUC) approved plans to add around 25,500 MW of renewable energy resources and 15,000 MW of energy storage and demand response resources by 2032.  The decision also adopted a 35 million metric ton (MMT) electric sector greenhouse gas emission (GHG) planning target. That goal, also to be achieved by 2032, is tighter than an earlier 46 […]

La enseñanza bíblica en profundidad de John MacArthur lleva la verdad transformadora de la Palabra de Dios a millones de personas cada día.

La enseñanza bíblica en profundidad de John MacArthur lleva la verdad transformadora de la Palabra de Dios a millones de personas cada día.

La enseñanza bíblica en profundidad de John MacArthur lleva la verdad transformadora de la Palabra de Dios a millones de personas cada día.

11757b installation manual calibration user guide

Pressure on the San Andreas fault from a now-dried lake could have been sufficient to trigger past major earthquakes in California. The lake’s disappearance could explain why there have been no such quakes for nearly 300 years

In support of their AI ambitions, tech companies are rapidly expanding US data centres, and this growth is on track to significantly increase US gas demand by 2030